Life Sciences Commons^™

Thesis Document 07172017 Revised.Pdf, Jesse Gray

Jesse Gray

Structure And Activity Of Metallo-Peptides, Christian C. Tang

USF Tampa Graduate Theses and Dissertations

Metal ions are ubiquitously found in all living systems and play vital roles in supporting life forms by performing an array of biological activities. Such biological activities include binding and transforming organic molecules, and also acting as active centers and cofactors for catalysis of various acid-base and redox reactions in biological system. The main focus in bioinorganic chemistry is to elucidate the structural and functional roles of metals in biological systems. Among all transition metal ions, Cu²⁺ and Fe³⁺ are especially versatile and important due to their abilities to go through redox efficiently.

This dissertation can be divided …

Heavy Metals In Seaweeds From A Polluted Estuary In Coastal Maine, Ian D. Medeiros, Arthur C. Mathieson, Nishanta Rajakaruna

Biological Sciences

Seaweeds (macroalgae) accumulate heavy metals from seawater and can therefore serve as biological monitors of marine pollution. In this paper, we present data on the tissue chemistry of seaweeds from the area of Callahan Mine, an intertidal copper and zinc mine in Brooksville, ME, USA, abandoned in the 1970s and now designated as a Superfund site. The mine is located on the Goose Pond estuary, which drains into the ocean via a tidal waterfall at Goose Falls. Seaweed tissue samples from this estuary had the same relative concentrations of metals as had been reported previously in water samples from this …

The Double-Edges Role Of Copper In The Fate Of Amyloid Beta In The Presence Of Anti-Oxidants, Hui Ye, Jing Yang, Xueli Zhang, Yiying Zhu, Emily Lenzowski, Yanli Tian, Can Zhang, Markus Hardt, Chunhua Qiao, Rudolph E. Tanzi, Anna Moore, Chongzhao Ran

Biology: Faculty Publications and Other Works

The biological fate of amyloid beta (Aβ) species is a fundamental question in Alzheimer’s disease (AD) pathogenesis. The competition between clearance and aggregation of Aβs is critical for the onset of AD. Copper has been widely considered to be an inducer of harmful crosslinking of Aβs, and an important triggering factor for the onset of AD. In this report, however, we present data to show that copper can also be an inducer of Aβ degradation in the presence of a large excess of well-known intrinsic (such as dopamine) or extrinsic (such as vitamin C) anti-oxidants. The degraded fragments were identified …

Evaluating The Long Term Effects Of Continuous Broiler Litter Applications On Residual Soil Fertility And Yields Of Corn And Soybean Crops, And A Side Experiment Studying The Residual Copper Fertilization Effects On These Crops, Jesse Lee Gray Mr.

Murray State Theses and Dissertations

Abstract

The increasing cost of commercial fertilizers and the availability of animal waste that has high nutrient content have led to producers utilizing animal waste such as poultry litter in their fertility programs. This increased use has raised concerns as to what are the optimum rates to apply to meet crop needs and how will long term use at high rates of litter effect soil test levels of nutrients such as phosphorus and potassium. The objective of this study was to address these concerns. This study has been ongoing since 1997 with treatments of zero to seven tons of litter …

Bioaccumulation And Oxidative Stress Impact Of Pb, Ni, Cu, And Cr Heavy Metals In Two Bryophyte Species, Pleurochaete Squarrosa And Timmiella Barbuloides, Serap Aydoğan, Bengi̇ Erdağ, Lale Aktaş

Turkish Journal of Botany

This study was conducted to evaluate the bioaccumulation and oxidative stress impact of lead (Pb), nickel (Ni), copper (Cu), and chromium (Cr) in bryophyte species Pleurochaete squarrosa and Timmiella barbuloides exposed to Pb, Ni, Cu, and Cr at 1 mM concentration for 48 h. Bioaccumulation ratios in the non-Fenton metals Ni and Pb were found to be higher than in Fenton metals Cu and Cr for both moss species. Except for Cu, high levels of heavy metal accumulation caused a slight increase in dry weights of both species. Total chlorophyll and carotenoid contents of both species showed variations under Ni, …

The Effect Of Various Inducers And Their Combinations With Copper On Laccaseproduction Of Trametes Versicolor Pellets In A Repeated-Batch Process, Emre Bi̇rhanli, Özfer Yeşi̇lada

Turkish Journal of Biology

The aim of this study was to increase laccase production in Trametes versicolor ATCC 200801 pellets by using various inducers and their combinations under repeated-batch conditions. Because copper is an effective inducer for laccase production, the effect of Cu on laccase production in this strain was tested first. The optimal Cu concentration for the highest laccase production was 0.5 mM. Following this preliminary study, the effect of other inducers [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), syringaldazine, guaiacol, and 2,5-xylidine] on laccase production was determined. Copper was determined to be the most efficient inducer among the inducers used. Therefore, the synergistic effect of each …

Quantification Of Copper Content With Laser Induced Breakdown Spectroscopy As A Potential Indicator Of Offal Adulteration In Beef, Maria Piedad Casado-Gavalda, Yash Dixit, David Geulen, Raquel Cama-Moncunill, Xavier Cama-Moncunill, Maria Markiewicz-Keszycka, Patrick J. Cullen, Carl Sullivan

Articles

Laser induced breakdown spectroscopy (LIBS) is an emerging technique in the field of food analysis which provides various advantages such as minimal sample preparation, chemical free, rapid detection, provision of spatial information and portability. In this study, LIBS was employed for quantitative analysis of copper content in minced beef samples spiked with beef liver over three independent batches. Copper content was determined with graphite furnace atomic absorption spectroscopy (GFAAS) in order to obtain reference values for modelling. Partial least square regression (PLSR) was performed to build a calibration and validation model. A calibration model with a high Rcv2 of 0.85 …

On The Origin Of Superoxide Dismutase: An Evolutionary Perspective Of Superoxide-Mediated Redox Signaling., Adam J. Case

Journal Articles: Cellular & Integrative Physiology

The field of free radical biology originated with the discovery of superoxide dismutase (SOD) in 1969. Over the last 5 decades, a plethora of research has been performed in species ranging from bacteria to mammals that has elucidated the molecular reaction, subcellular location, and specific isoforms of SOD. However, while humans have only begun to study this class of enzymes over the past 50 years, it has been estimated that these enzymes have existed for billions of years, and may be some of the original enzymes found in primitive life. As life evolved over this expanse of time, these enzymes …

Isolation And Spectroscopic Characterization Of Zn(Ii), Cu(Ii), And Pd(Ii) Complexes Of 1,3,4-Thiadiazole-Derived Ligand, Dariusz Karcz, Arkadiusz Matwijczuk, Bozena Boron, Bernadette Creaven, Leszek Fiedor, Andrzej Niewiadomy, Mariusz Gagos

Articles

A series of complexes incorporating Zn(II), Cu(II), and Pd(II) ions, and 4-(5-heptyl-1,3,4-thiadiazol-2-yl)benzene-1,3-diol (L1) as model ligand, was synthesized in order to examine the nature of potential interactions between biologically active ligands, 1,3,4-thiadiazoles and metal ions with proven biological relevance. The structures of the compounds isolated were characterized using a number of spectroscopic methods including IR, Uv–vis, AAS, steady state and time-resolved fluorescence (TRF). The results obtained suggest that the L1-Zn(II) and L1-Pd(II) complexes consist of one molecule of L1 and one acetate ion acting as ligands, while the L1-Cu(II) complex adapts a 2:1 (L1: metal) stoichiometry. The coordination of L1 …

Assessment Of Sampling Technique From Feeders On Copper, Zinc, Calcium, And Phosphorus Analysis, A. M. Jones, J. C. Woodworth, C. Vahl, S. S. Dritz, M. D. Tokach, R. D. Goodband, J. M. Derouchey

Kansas Agricultural Experiment Station Research Reports

While numerous research articles have been published on how to collect a representative sample, and analytical or laboratory-to-laboratory variation, we are unaware of any studies to examine exactly how many samples to collect from feeders, or whether they should be pooled or not to minimize analytical variation. Therefore, this study was designed to evaluate different sampling procedures and the number of samples to collect, and achieve an accurate assessment of nutrient fortification in swine diets.

Diet samples were collected from a study evaluating the effects of increasing Cu on growth performance of finishing pigs. Treatments were arranged in a split-plot …

Effects Of Increasing Copper From Tri-Basic Copper Chloride Or A Copper-Chelate On Growth Performance Of Nursery Pigs, C. Carpenter, J. C. Woodworth, J. M. Derouchey, M. D. Tokach, R. D. Goodband, S. S. Dritz, J. L. Usry

Kansas Agricultural Experiment Station Research Reports

A total of 1,452 pigs [Group 1; 350 barrows (DNA 200 × 400; initially 13.2 lb)] and [Group 2; 1,102 barrows and gilts (PIC 1050 × 280; initially 13.3 lb)] were used to determine the effects of added Cu source and level on nursery pig performance. There were 5 pigs per pen and 10 replications per treatment in group 1, and 24 to 27 pigs per pen and 6 replications per treatment in group 2. Pens of pigs were allotted by BW to 1 of 7 dietary treatments arranged as a 2 × 3 factorial plus a control diet, with …

Effects Of A Combination Of Essential Oils (Victus Liv), Increased Zinc Oxide And Copper Sulfate, Or Their Combination In Nursery Diets On Pig Performance, A. Langemeier, J. Morton, S. Scotten, M. T. Thayer, J. L. Nelssen

Kansas Agricultural Experiment Station Research Reports

A total of 280 weaned pigs (Line 600 × 241; DNA, Columbus, NE; initially 11.4 lb) were used in a 35-d study to compare the effects of feeding growth promoting alternatives (added trace minerals, copper and zinc, or essential oils (Victus LIV; DSM Nutritional Products, Parsippany, NJ), alone or in combination, on nursery pig performance in comparison to Carbadox, (Mecadox, Phibro Animal Health, Teaneck, NJ). Pigs were allotted to 1 of 7 dietary treatments in pens of 5 at weaning in a randomized complete block design with 8 replications per treatment. Dietary treatments were arranged with a negative control diet …

Evaluation Of Elarom Ses With Or Without Tri-Basic Copper Chloride On Nursery Pig Growth Performance, H. Williams, J. C. Woodworth, J. M. Derouchey, S. S. Dritz, M. D. Tokach, R. D. Goodband

Kansas Agricultural Experiment Station Research Reports

A total of 360 weanling pigs (DNA 200 × 400; initially 11.5 lb BW) were used in a 42-d study evaluating the effects of feeding Elarom SES in combination with increasing tri-basic copper chloride on nursery pig performance and fecal consistency. Elarom SES (Trouw Nutrition USA, Highland, IL) is a specialty feed ingredient containing a blend of active ingredients designed to enhance growth performance and gut health. Tri-basic copper chloride (TBCC, Intellibond C, Micronutrients USA, LLC., Indianapolis, IN) is a form of copper (Cu) that has the potential for improved bioavailability and enhanced growth performance. Pigs were weaned at approximately …