Life Sciences Commons^™

Gene Signature Of Stromal Cells Which Support Dendritic Cell Development., Geneviève Despars, Pravin Periasamy, Jonathan Tan, Janice Abbey, Terence O'Neill, Helen O'Neill

Jonathan Tan

Spleen stromal cells are critical determinants of dendritic cell (DC) development in spleen. The spleen stromal line, namely STX3, supports DC differentiation in vitro from overlaid bone marrow cells while the lymph node stromal line, namely 2RL22, does not. Here we have characterised the hematopoietic support capacity of each stroma, and analyzed lineage origin of the stromal cell lines by gene profiling using microarrays. Stromal co-culture experiments were performed using bone marrow cells as a source of hematopoietic progenitors. A characteristic immature myeloid-like CD11c+CD11b+CD86+MHC-II−/loB220−CD8α− DC is produced after 14 days in STX3 cocultures, while 2RL22 cocultures produce only monocyte/macrophage-like cells. …

North Atlantic Vibrio Vulnificus Surveillance From Postharvest Oysters At A Us Shellfish Processing Facility, Kenneth Lavalley, Joseph Dealteris, Michael Rice, Marta Gomez-Chiarri

Marta Gomez-Chiarri

Postharvest surveillance for Vibrio vulnificus by a commercial processing facility was conducted from May 2001 to September 2003. Harvest areas included the Delaware Bay, Long Island Sound and Prince Edward Island. Occurrence followed a seasonal distribution. Low densities were observed in June, increased through August, and became rare by September. Given the ubiquitous nature of marine Vibrios, it was not surprising to find V. vulnificus everywhere, which was investigated. Observations confirmed the importance of strict time/temperature and product handling practices by shellfish harvesters, dealers and processors. Mishandling led to a potentially dangerous and uncharacteristically high V. vulnificus observation.

A Simple Spectrophotometric Streptavidin-Biotin Binding Assay Utilizing Biotin-4-Fluorescein., Mark Waner, David Mascotti

Mark J. Waner

A new assay for biotin binding capacity of Streptavidin (SA) is presented in this work. The assay is based on the large decrease in the extinction coefficient at 493 nm that accompanies binding of biotin-4-fluorescein (B4F) to SA. This decrease is attributed to formation of a charge transfer complex between the B4F-donor and one or more SA residues. We show that one may observe the stoichiometric binding via monitoring the absorbance at 493 nm using either SA or B4F as the titrant. The sensitivity of the assay is at the lower end of similar fluorimetric and photometric assays. Though the …

Proteome Of Geobacter Sulfurreducens Through Genome-Wide Expression Data And Sequence Analysis, Derek Lovley, Yan-Huai Ding, Kim Hixson, Muktak Aklujkar, Mary Lipton, Richard Smith, Tϋnde Mester

Muktak Aklujkar

No abstract provided.

Laser-Induced Photon-Branched Chain Reaction In A Chemically-Active Gas-Dispersed Medium, Thomas F. George, Renat R. Letfullin, Galen C. Duree

Thomas George

Insig2 Gene Polymorphism Is Associated With Increased Subcutaneous Fat In Women And Poor Response To Resistance Training In Men, Funda Orkunoglu-Suer, Heather Gordish-Dressman, Priscilla Clarkson, Paul Thompson, Theodore Angelopoulos, Paul Godron, Niall Moyna, Linda Pescatello, Paul Visich, Robert Zoeller, Brennan Harmon, Richard Seip, Eric Hoffman, Joseph Devaney

Priscilla M. Clarkson

Background A common SNP upstream of the INSIG2 gene, rs7566605 (g.-10,1025G>C, Chr2:118,552,255, NT_022135.15), was reported to be associated with obesity (Body Mass Index, [BMI]) in a genome-wide association scan using the Framingham Heart Study but has not been reproduced in other cohorts. As BMI is a relatively insensitive measure of adiposity that is subject to many confounding variables, we sought to determine the relationship between the INSIG2 SNP and subcutaneous fat volumes measured by MRI in a young adult population. Methods We genotyped the INSIG2 SNP rs7566605 in college-aged population enrolled in a controlled resistance-training program, (the Functional Polymorphism …

Gokilaht®-S 5ec Testing On Culex Quinquefasciatus Say Larvae For An Early Detection In Esterase And Monooxygenase Resistance System, Soam Prakash, Gavendra Singh

Soam Prakash

Giant Sequoia Insect, Disease, And Ecosystem Interactions, Douglas D. Piirto

Douglas D. Piirto

Individual trees of giant sequoia (Sequoia gigantea [Lindl.] Decne.) have demonstrated a capacity to attain both a long life and very large size. It is not uncommon to find old-growth giant sequoia trees in their native range that are 1,500 years old and over 15 feet in diameter at breast height. The ability of individual giant sequoia trees to survive over such long periods of time has often been attributed to the species high resistance to disease, insect, and fire damage. Such a statement, however, is a gross oversimplification, given broader ecosystem and temporal interactions. For example, why isn't there …

Efficacy Of Herbicide Application Methods Used To Control Tanoak (Lithocarpus Densiflorus) In An Uneven-Aged Coast Redwood Management Context, Douglas D. Piirto, Brenda Smith, Eric K. Huff, Scott T. Robinson

Douglas D. Piirto

Three methods of tanoak (Lithocarpus densiflorus [Hook. & Arn.] Rehd.) control involving the application of the amine or ester form of triclopyr were evaluated in this coast redwood uneven-aged forest management study of herbicides. A cut-stump application with the amine form of triclopyr (Garlon 3A), frill cut with the amine form of triclopyr, basal-bark (outer surface) with the ester form of triclopyr (Garlon 4), and an untreated control were replicated three times. The tanoak control results in Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco) and/ or coast redwood (Sequoia sempervirens [D. Don] Endl.) predominated stands obtained in earlier studies …

Cost-Effective Fire Management For Southern California's Chaparral Wilderness: An Analytical Procedure, Chris A. Childers, Douglas D. Piirto

Douglas D. Piirto

Fire management has always meant fire suppression to the managers of the chaparral covered southern California National Forests. Today, Forest Service fire management programs must be cost effective, while wilderness fire management objectives are aimed at recreating natural fire regimes. A cost-effectiveness analysis has been developed to compare fire management options for meeting these objectives in California's chaparral wilderness. This paper describes the analytical procedure using examples from a study currently being conducted for the Los Padres National Forest, and discusses some preliminary results.

Sourwood: An Apiforestation Story, Tammy Horn

Tammy Horn

No abstract provided.

Co-Encapsulation Of Gallium With Gentamicin In Liposomes Enhances Antimicrobial Activity Of Gentamicin Against Pseudomonas Aeruginosa, Majed Halwani, B. Yebio, Zacharias E. Suntres, M. Alipour, Ali Azghani, A. Omri

Ali Azghani

Distribution Patterns Of Sciurus Niger (Eastern Fox Squirrel) Leaf Nests Within Woodlots Across A Suburban/Urban Landscape, Carmen M. Salsbury

Carmen M. Salsbury

To determine habitat characteristics that influence Sciurus niger (Eastern Fox Squirrel) abundance and distribution within a suburban/urban landscape in the midwestern United States, I documented the density and placement of fox squirrel leaf nests in 20 woodlots in the Indianapolis metropolitan area. Marion County, IN. The woodlots varied in size (0.94 to 19.5 ha), approximate age, shape, and degree of isolation from other woodlots and suitable squirrel habitat in the surrounding area. Only 8.0% of nests were located in a tree with another nest, and nests were randomly distributed in all but one woodlot, where they were uniformly dispersed. Nest …

Objectively Measured Physical Activity In Urban Alternative High School Students, John R. Sirard, Martha Y. Kubik, Jayne A. Fulkerson, Chrisa Arcan

John Sirard

Introduction—Alternative high school students are an underserved population of youth at greater risk for poor health behaviors and outcomes. Little is known about their physical activity patterns. Purpose—The purpose of this study was to describe 1) physical activity levels of students attending alternative high schools (AHS) in St. Paul/Minneapolis, MN and 2) compliance with wearing a physical activity accelerometer. Methods—Sixty-five students (59% male, 65% <18 years>old, 51% African-American, 17% Caucasian, 32% mixed and other) wore an accelerometer during all waking hours for seven days as part of the baseline assessment for a school-based physical activity and dietary behavior intervention. Accelerometer data …

In Vivo Lumbo-Sacral Forces And Moments During Constant Speed Running At Different Stride Lengths, Joseph Seay, W. Scott Selbie, Joseph Hamill

Joseph Hamill

The aim of this study was to introduce a Newton–Euler inverse dynamics model that included reaction force and moment estimation at the lumbo-sacral (L5-S1) and thoraco-lumbar (T12-L1) joints. Data were collected while participants ran over ground at 3.8 m s71 at three different stride lengths: preferred stride length, 20% greater than preferred, and 20% less than preferred. Inputs to the model were ground reaction forces, bilateral lower extremity and pelvis kinematics and inertial parameters, kinematics of the lumbar spine and thorax and inertial parameters of the lumbar segment. Repeated measures ANOVA were performed on the lower extremity sagittal kinematics and …

Melamine Contamination Of Infant Formula In China: The Causes, Food Safety Issues And Public Health Implications, Emmanuel Ohene Afoakwa

Professor Emmanuel Ohene Afoakwa

No abstract provided.

Efficacy Of Bacillus Sphaericus Against Larvae Of Malaria And Filarial Vectors: An Analysis Of Early Resistance Detection, Soam Prakash, Gavendra Singh

Soam Prakash

The Internet Archive: How You Can Look Into The Web’S Past And Plan Your Future, Joe Zumalt

Joseph R. Zumalt

How to find historical pages on the World Wide Web with the Internet Archive.

Impact Of An External Energy On Staphylococcus Epidermis [Atcc –13518] In Relation To Antibiotic Susceptibility And Biochemical Reactions – An Experimental Study, Mahendra Kumar Trivedi, Shrikant Patil

Mahendra Kumar Trivedi

Web-Based Arabidopsis Functional And Structural Genomics Resources, Yan Lu, Robert Last

Yan Lu

No abstract provided.

Reflections On Recycling, Pollution And History Or, How To Beat The High Cost Of Living, Richard B. Philp

Richard B. Philp

Lessons learned during the Great Depression and World War II may have to be relearned. The three Rs (reduce, reuse and recycle) were essential then to individual and national survival. A fourth R, repair, was practised as well. These lessons, if relearned, will stand us in good stead when dealing with the current economic climate as well as with problems of pollution and global warming.

In Vitro Clonal Propagation Of Bael (Aegle Marmelos Corr.) Cv. Cishb1 Through Enhanced Axillary Branching, Rajesh Pati

Rajesh Pati

Rapid clonal micropropagation protocol of Aegle marmelos (L.) Corr. cv. CISH-B1 was achieved by nodal stem segment of mature bearing tree. Three centimeter long shoots having one axillary bud excised from 10-15th nodal region of shoots during September gave quick in vitro bud burst (5.33 days) when cultured on MS medium supplemented with BAP, 8.84 μM + IAA 5.7 μM. The maximum number of proliferated shoots (9.0/explant) were obtained on same medium supplemented with BAP 8.84 μM + IAA 5.7 μM. The micro shoots were rooted (100 %) on ½ strength MS medium supplemented with IBA 49.0 + IAA 5.7 …

Effects Of Oral Consumption Of The Green Tea Polyphenol Egcg In A Murine Model For Human Sjogren's Syndrome, An Autoimmune Disease., K. Gillespie, I. Kodani, D. Dickinson, K. Ogbureke, A. Camba, M. Wu, S. Looney, Tin-Chun Chu, H. Qin, F. Bisch, M. Sharawy, G. Schuster, S. Hsu

Tin-Chun Chu, Ph.D.

No abstract provided.

Demographic Parameters Of Yellowfin Croaker, Umbrina Roncador (Perciformes: Sciaenidae), From The Southern California Bight, Daniel Pondella, John Froeschke, Lynne Wetmore, Eric Miller, Charles Valle, Lea Medeiros

Daniel Pondella

The yellowfin croaker, Umbrina roncador Jordan & Gilbert, 1882, is a common nearshore and surf-zone species in the southern California bight. Age was determined for individuals (n = 1,209) using annual increments in otoliths, and size at age was modeled using the von Bertalanffy growth curve (L∞ = 307.754 mm, k = 0.278 yr−1, t0 = −0.995 yr; maximum age = 15 yr). Females (L∞= 313.173 mm, k = 0.307 yr−1, t0 = −0.771 yr) grew significantly faster and larger than males (L∞= 298.886, k = 0.269 yr−1, t0 = −1.072 yr). Age and growth modeling based upon otolith length …

Mexican Jay (Aphelocoma Ultramarina), John Mccormack, L. Brown

John E. McCormack

No abstract provided.

Stress-Induced Attenuation Of Acoustic Startle In Low-Saccharin-Consuming Rats., Clinton Chapman, Nancy Dess, Mitzi Gonzales, Cameryn Garrett

Clinton D Chapman

Exposure to stress can lead to either increased stress vulnerability or enhanced resiliency. Laboratory rats are a key tool in the exploration of basic biobehavioral processes underlying individual differences in the effect of stress on subsequent stressors' impact. The Occidental low (LoS) and high (HiS) saccharin-consuming rats, which differ in emotional reactivity, are useful in this effort. In the present study, footshock affected acoustic startle amplitude 4 h later among LoS but not HiS rats. Surprisingly, shock attenuated startle rather than sensitizing it, a finding not previously reported for male rats exposed to shock. Attenuation was blocked by administering the …

Quorum-Sensing Signals In The Microbial Community Of The Cabbage White Butterfly Larval Midgut, Bradley R. Borlee, Grant D. Geske, Courtney Jaime Robinson, Helen E. Blackwell, Jo Handelsman

Courtney Robinson

Modelling Tempering Behaviour Of Dark Chocolates From Varying Particle Size Distribution And Fat Content Using Response Surface Methodology, Emmanuel Ohene Afoakwa, Alistair Paterson, Mark Fowler, Joselio Vieira

Professor Emmanuel Ohene Afoakwa

Central Composite Rotatable Design (CCRD) for K=2 was used to study the combined effects of multi-stage heat exchangers for Stages 1 (14–30 °C) and 2 (12–28 °C) coolant temperatures at constant Stage 3 coolant and holding temperatures during tempering of dark chocolates using laboratory-scale mini-temperer. Quantitative data on chocolate temper index (slope) were obtained for products with varying particle size distribution (PSD) (D90 of 18, 25, 35 and 50 μm) and fat (30% and 35%) content. Regression models generated using stepwise regression analyses were used to plot response surface curves, to study the tempering behaviour of products. The results showed …

A Transcendental To Changing Metal Powder Characteristics, Mahendra Kumar Trivedi, Rama Mohan Tallapragada

Mahendra Kumar Trivedi

• https://www.trivedieffect.com/science/a-transcendental-approach-to-changing-metal-powder-characteristics
• https://www.sciencedirect.com/science/article/pii/S0026065708701450

A Darwinist View Of The Living Constitution, Scott Dodson

Scott Dodson

The metaphor of a “living" Constitution imports terms from biology into law and, in the process, relies on biology for its meaning. A proper understanding of biology is therefore central to understanding living constitutionalism. Yet despite its rampant use by both opponents and proponents of living constitutionalism, and despite the current fervent debate over whether biology can be useful to the law, no one has evaluated the metaphor from a biological perspective.

This Essay begins that inquiry in an interdisciplinary study of law, science, and philology. The Essay first evaluates the metaphor as it is currently used and concludes that …