Open Access. Powered by Scholars. Published by Universities.®
- Institution
-
- Selected Works (8)
- University of Nebraska - Lincoln (7)
- Old Dominion University (6)
- Aga Khan University (3)
- Iowa State University (3)
-
- Loyola University Chicago (3)
- Wayne State University (3)
- Dartmouth College (2)
- Illinois Wesleyan University (2)
- University of Montana (2)
- Cleveland State University (1)
- Marshall University (1)
- University of Massachusetts Amherst (1)
- University of the Pacific (1)
- Utah State University (1)
- Valparaiso University (1)
- Western Michigan University (1)
- William & Mary (1)
- Keyword
-
- Isoenzymes (2)
- Methotrexate (2)
- 5-biphosphate carboxylase (1)
- <i>Chlamydomonas reinhardtii</i> (1)
- AIDS (Disease) (1)
-
- Adrenal glands (1)
- Air pollutants (1)
- Animals (1)
- Balanced polymorphism (1)
- Base sequence (1)
- Biochemical (1)
- Blotting (1)
- Carrier proteins (1)
- Cell line (1)
- Cells (1)
- Chloramphenicol o-acetyltransferase (1)
- Chromatography (1)
- Chronic renal failure (1)
- Circular dichroism (1)
- Collaboration (1)
- Cyclic AMP (1)
- Cytotoxicity (1)
- DNA -- Analysis (1)
- Dna probes (1)
- Dolichol phosphates (1)
- Drug effects (1)
- Energy resources (1)
- Environmental assessment (1)
- Environmental legislation (1)
- Enzyme induction (1)
- Publication
-
- David P. Mascotti (5)
- Department of Biochemistry: Faculty Publications (5)
- Chemistry & Biochemistry Theses & Dissertations (4)
- Biochemistry and Molecular Biology Faculty Publications (3)
- David J. Oliver (3)
-
- Department of Biological & Biomedical Sciences (3)
- Bioelectrics Publications (2)
- Chemistry and Biochemistry Faculty Publications (2)
- Dartmouth Scholarship (2)
- David Bollivar (2)
- David Hage Publications (2)
- Dissertations (2)
- Scholarship (2)
- All Dugoni School of Dentistry Faculty Articles (1)
- All Graduate Theses and Dissertations, Spring 1920 to Summer 2023 (1)
- Biological Sciences Faculty Research (1)
- Biology Faculty Publications (1)
- Chemistry: Faculty Publications and Other Works (1)
- Dissertations, Theses, and Masters Projects (1)
- Gail Gasparich (1)
- Lila Gierasch (1)
- Masters Theses (1)
- Physics Faculty Publications (1)
- Publication Type
- File Type
Articles 1 - 30 of 47
Full-Text Articles in Life Sciences
Purification And Ligand Binding Of A Soluble Class I Mhc Molecule Consisting Of The First Three Domains Of H-2kd Fused To B2-Microglobulin Expressed In The Baculovirus/Insect Cell System, Francois Godeau, Immanuel F. Luescher, David M. Ojcius, Cecile Saucier, Estelle Mottez, Lucien Cabanie, Philippe Kourilsky
Purification And Ligand Binding Of A Soluble Class I Mhc Molecule Consisting Of The First Three Domains Of H-2kd Fused To B2-Microglobulin Expressed In The Baculovirus/Insect Cell System, Francois Godeau, Immanuel F. Luescher, David M. Ojcius, Cecile Saucier, Estelle Mottez, Lucien Cabanie, Philippe Kourilsky
All Dugoni School of Dentistry Faculty Articles
A recombinant baculovirus encoding a single-chain murine major histocompatibility complex class I molecule in which the first three domains of H-2Kd are fused to beta 2-microglobulin (beta 2-m) via a 15-amino acid linker has been isolated and used to infect lepidopteran cells. A soluble, 391-amino acid single-chain H-2Kd (SC-Kd) molecule of 48 kDa was synthesized and glycosylated in insect cells and could be purified in the absence of detergents by affinity chromatography using the anti-H-2Kd monoclonal antibody SF1.1.1.1. We tested the ability of SC-Kd to bind antigenic peptides using a direct binding assay based on photoaffinity labeling. The photoreactive derivative …
Isolation And Characterization Of The Tricarboxylate Transporter From Pea Mitochondria, Cecilia A. Mcintosh, David J. Oliver
Isolation And Characterization Of The Tricarboxylate Transporter From Pea Mitochondria, Cecilia A. Mcintosh, David J. Oliver
David J. Oliver
The tricarboxylate transporter was solubilized from pea (Pisum sativum) mitochondria with Triton X-114, partially purified over a hydroxylapatite column, and reconstituted in phospholipid vesicles. The proteoliposomes exchanged external [14C]citrate for internal citrate or malate but not for preloaded D,L-isocitrate. Similarly, although external malate, succinate, and citrate competed with [14C]citrate in the exchange reaction, D,L-isocitrate and phosphoenolpyruvate did not. This tricarboxylate transporter differed from the equivalent activity from animal tissues in that it did not transport isocitrate and phosphoenolpyruvate. In addition, tricarboxylate transport in isolated plant mitochondria, as well as that measured with the partially purified and reconstituted transporter, was less …
Identification Of The Nd Δ And Σ States And The 1,3Φ←←X 3Σ− G Transition Of O2 By Resonant Multiphoton Ionization, Robert J. Yokelson, R. J. Lipert, W. A. Chupka
Identification Of The Nd Δ And Σ States And The 1,3Φ←←X 3Σ− G Transition Of O2 By Resonant Multiphoton Ionization, Robert J. Yokelson, R. J. Lipert, W. A. Chupka
Chemistry and Biochemistry Faculty Publications
Spectra of the 3dRydberg state region of O2 have been obtained by two‐photon resonant ionization of the ground electronic state. By varying the rotational distribution and radiation polarization, all observed bands were identified and attributed to excitation of Σ, Δ, and Φ states. Earlier assignments were corrected. The Δ and Φ assignments are complete while the Σ assignments are so far incomplete.
Identification Of The Ns And Nd Rydberg States Of O2 For N=3–5, Robert J. Yokelson, R. J. Lipert, W. A. Chupka
Identification Of The Ns And Nd Rydberg States Of O2 For N=3–5, Robert J. Yokelson, R. J. Lipert, W. A. Chupka
Chemistry and Biochemistry Faculty Publications
The 4s‐3d and 5s‐4dRydberg complexes of diatomic oxygen have been studied by (2+1) resonance‐enhanced multiphoton ionization of the X 3∑ g − ground state of O2. We have located and identified at least two vibrational levels of each of the following states: Three of four expected 4sσ Π states; all four expected 5sσ Π states; 18 of 22 expected 3d states (with only the states of the 3dσ orbital remaining unobserved); and 5 of the 10 predicted 4dπ states. State assignments …
Circular Dichroism And Molecular Modeling Yield A Structure For The Complex Of Human Immunodeficiency Virus Type 1 Trans-Activation Response Rna And The Binding Region Of Tat, The Trans-Acting Transcriptional Activator, Erwann P. Loret, Philippe T. Georgel, W. Curtis Johnson Jr., Pui Shing Ho
Circular Dichroism And Molecular Modeling Yield A Structure For The Complex Of Human Immunodeficiency Virus Type 1 Trans-Activation Response Rna And The Binding Region Of Tat, The Trans-Acting Transcriptional Activator, Erwann P. Loret, Philippe T. Georgel, W. Curtis Johnson Jr., Pui Shing Ho
Biological Sciences Faculty Research
Transcription in the human immunodeficiency virus type 1 (HIV-1) retrovirus is regulated by binding the viral Tat protein (trans-acting transcriptional activator) to the trans-activation response (TAR) RNA sequence. Here, vacuum UV circular dichroism (VUV-CD) is used to study the structure of TAR and its complex with two peptide fragments that are important for Tat binding to TAR. The VUV-CD spectrum of TAR is typical of A-form RNA and is minimally perturbed when bound to either the short or the long Tat peptide. The CD spectra ofthe complexes indicate an extended structure in the argnine-rich region of Tat from amino acid …
Structural Characterization Of Fucoidan And Its Role In The Hemizona Assay, Manish S. Patankar
Structural Characterization Of Fucoidan And Its Role In The Hemizona Assay, Manish S. Patankar
Chemistry & Biochemistry Theses & Dissertations
Fucoidan is a polysaccharide commonly found in brown algae. It is a sulfated polymer of the monosaccharide L-fucose. Fucoidan is thought to function in water retention by algae. Commercially available fucoidan is extracted from a brown algae called Eurus vesiculosus.
This polysaccharide has immense potential as a biologically active agent: (i) it exhibits anticoagulant action, (ii) it has also been reported to block retroviral replication and (iii) it is capable of blocking sperm-egg binding in sea urchin, mouse and rabbit. Recently, fucoidan has also been shown to block the interaction of human sperm and egg in the hemizona assay …
Thermodynamics Of Single-Standard Rna-Binding To Oligolysines Containing Tryptophan, David Mascotti, Timothy Lohman
Thermodynamics Of Single-Standard Rna-Binding To Oligolysines Containing Tryptophan, David Mascotti, Timothy Lohman
David P. Mascotti
These studies provide important comparative information for the interpretation of the thermodynamics of protein-ss nucleic acid interaction.
The Structure Of A Complex Of Bovine Ɑ-Thrombin And Recombinant Hirudin At 2.8-Å Resolution, Jacqueline Vitali, Philip D. Martin, Michael G. Malkowski, William D. Robertson, Jerome B. Lazar, Richard C. Winant, Paul H. Johnson, Brian Fp Edwards
The Structure Of A Complex Of Bovine Ɑ-Thrombin And Recombinant Hirudin At 2.8-Å Resolution, Jacqueline Vitali, Philip D. Martin, Michael G. Malkowski, William D. Robertson, Jerome B. Lazar, Richard C. Winant, Paul H. Johnson, Brian Fp Edwards
Biochemistry and Molecular Biology Faculty Publications
Crystals of the complex of bovine alpha-thrombin with recombinant hirudin variant 1 have space group C222(1) with cell constants a = 59.11, b = 102.62, and c = 143.26 A. The orientation and position of the thrombin component was determined by molecular replacement and the hirudin molecule was fit in 2 magnitude of Fo - magnitude of Fc electron density maps. The structure was refined by restrained least squares and simulated annealing to R = 0.161 at 2.8-A resolution. The binding of hirudin to thrombin is generally similar to that observed in the crystals of human thrombin-hirudin. Several differences in …
The Structure Of A Complex Of Bovine &-Thrombin And Recombinant Hirudin At 2.8-A Resolution, Jacqueline Vitali, Philip D. Martin, Michael G. Malkowski, William D. Robertson, Jerome B. Lazar, Richard C. Winant, Paul H. Johnson, Brian F.P. Edwards
The Structure Of A Complex Of Bovine &-Thrombin And Recombinant Hirudin At 2.8-A Resolution, Jacqueline Vitali, Philip D. Martin, Michael G. Malkowski, William D. Robertson, Jerome B. Lazar, Richard C. Winant, Paul H. Johnson, Brian F.P. Edwards
Physics Faculty Publications
Crystals of the complex of bovine alpha-thrombin with recombinant hirudin variant 1 have space group C222(1) with cell constants a = 59.11, b = 102.62, and c = 143.26 A. The orientation and position of the thrombin component was determined by molecular replacement and the hirudin molecule was fit in 2 magnitude of Fo - magnitude of Fc electron density maps. The structure was refined by restrained least squares and simulated annealing to R = 0.161 at 2.8-A resolution. The binding of hirudin to thrombin is generally similar to that observed in the crystals of human thrombin-hirudin. Several differences in …
Nad+-Linked Isocitrate Dehydrogenase: Isolation, Purification, And Characterization Of The Protein From Pea Mitochondria, Cecilia A. Mcintosh, David J. Oliver
Nad+-Linked Isocitrate Dehydrogenase: Isolation, Purification, And Characterization Of The Protein From Pea Mitochondria, Cecilia A. Mcintosh, David J. Oliver
David J. Oliver
The NAD+-dependent isocitrate dehydrogenase from etiolated pea (Pisum sativum L.) mitochondria was purified more than 200-fold by dye-ligand binding on Matrix Gel Blue A and gel filtration on Superose 6. The enzyme was stabilized during purification by the inclusion of 20% glycerol. In crude matrix extracts, the enzyme activity eluted from Superose 6 with apparent molecular masses of 1400 ± 200, 690 ± 90, and 300 ± 50 kD. During subsequent purification steps the larger molecular mass species disappeared and an additional peak at 94 ± 16 kD was evident. The monomer for the enzyme was tentatively identified at 47 …
The Cpce And Cpcf Genes Of Synechococcus Sp. Pcc 7002. Construction And Phenotypic Characterization Of Interposon Mutants, J Zhou, G E. Gasparich, V L. Stirewalt, R De Lorimier, D A. Bryant
The Cpce And Cpcf Genes Of Synechococcus Sp. Pcc 7002. Construction And Phenotypic Characterization Of Interposon Mutants, J Zhou, G E. Gasparich, V L. Stirewalt, R De Lorimier, D A. Bryant
Gail Gasparich
Appearance Of A Methotrexate Binding Plasma Protein During High Dose Methotrexate Therapy, M P. Iqbal, I Malik, N Mehboobali
Appearance Of A Methotrexate Binding Plasma Protein During High Dose Methotrexate Therapy, M P. Iqbal, I Malik, N Mehboobali
Department of Biological & Biomedical Sciences
No abstract provided.
Isolation And Characterization Of The Triiodothyronine Regulatory Region Of The Fatty Acid Synthase Gene In Avian Species, Delfina Rivera Valencia
Isolation And Characterization Of The Triiodothyronine Regulatory Region Of The Fatty Acid Synthase Gene In Avian Species, Delfina Rivera Valencia
Masters Theses
Fatty acids are utilized for energy storage in the form of triglycerides or neutral fats. One way to regulate fatty acid biosynthesis is to regulate the activity of fatty acid synthase (FAS), the enzyme that catalyzes the reaction. Previous studies have shown that regulation of FAS activity and synthesis is regulated by the hormone triiodothyronine, T3, at the level of transcription. Thus, isolation and characterization of the promoter region o f the FAS gene will enable the identification of the thyroid hormone response elements (TREs). This study could shed light on the mechanisms on how this gene can …
Saccharomyces Cerevisiae Sec59 Cells Are Deficient In Dolichol Kinase Activity, Loree Heller, Peter Orlean, W. Lee Adair Jr.
Saccharomyces Cerevisiae Sec59 Cells Are Deficient In Dolichol Kinase Activity, Loree Heller, Peter Orlean, W. Lee Adair Jr.
Bioelectrics Publications
The temperature-sensitive Saccharomyces cerevisiae mutant sec59 accumulates inactive and incompletely glycosylated protein precursors in its endoplasmic reticulum at the restrictive temperature. O-mannosylation and glycosyl phosphatidylinositol membrane anchoring of protein are also abolished, consistent with a deficiency in dolichyl phosphate mannose. Membranes prepared from sec59 cells that had been shifted to the restrictive temperature, however, made normal amounts of dolichyl phosphate mannose when exogenous dolichyl phosphate was supplied, but dolichyl phosphate mannose synthesis was severely depressed in the absence of exogenous dolichyl phosphate. Quantitative measurements of dolichyl phosphate in sec59 cells showed that the levels were decreased to 48% of wild …
Kinetic Characterization Of A Recombinant C-Terminal Mutant Of Reverse Transcriptase From The Human Immunodeficiency Virus, Thomas S. Heard
Kinetic Characterization Of A Recombinant C-Terminal Mutant Of Reverse Transcriptase From The Human Immunodeficiency Virus, Thomas S. Heard
Chemistry & Biochemistry Theses & Dissertations
The human immunodeficiency virus (HIV) reverse transcriptase (RT) (EC 2.7.7.49) is the central replication enzyme for HIV. In general, the kinetic mechanism for this and all other polymerases involves the ordered binding of two substrates: a primer-template (PT) followed by a deoxyribonucleoside triphosphate (dNTP). Previous investigations prompted this research when it was discovered that the substrate dNTP, in absence of PT, could protect a recombinant c-terminal mutant HIV-1 RT from inhibition by pyridoxal-5'-monophosphate (PLP), an active-site dNTP inhibitor. In contrast, the non-mutant recombinant HIV-1 RT required both substrates for protection from PLP inhibition. This investigation sought to determine if this …
Biochemical Investigation Of Gout And Its Familial Incidences, Chin-Ling Hsu
Biochemical Investigation Of Gout And Its Familial Incidences, Chin-Ling Hsu
Chemistry & Biochemistry Theses & Dissertations
Gout is a chronic metabolic disorder caused by deposition of uric acid on the joint. It is categorized into two types: primary and secondary. Primary gout is uric acid overproduction, caused by excessive synthesis of the purine precursors. Secondary gout occurs also as the result of overproduction or decreased renal excretion of uric acid resulting from other disease processes or medication.
The two purine bases, hypoxanthine and xanthine, liberated from ribosides by the action of nucleoside phosphorylase, are degraded to uric acid as the final excretory product in the purine degradation pathway. Hypoxanthine and xanthine are the physiological substrates of …
Differential Regulation Of Collagenase Gene Expression By Retinoic Acid Receptors--Alpha, Beta And Gamma, Luying Pan, Stephen H. Chamberlain, David T. Auble, Constance E. Brinckerhoff
Differential Regulation Of Collagenase Gene Expression By Retinoic Acid Receptors--Alpha, Beta And Gamma, Luying Pan, Stephen H. Chamberlain, David T. Auble, Constance E. Brinckerhoff
Dartmouth Scholarship
The mechanisms involved in retinoic acid (RA)-mediated regulation of the collagenase gene in a rabbit synovial fibroblast cell line (HIG82) were investigated. When HIG82 cells are cotransfected with expression vectors containing cDNAs for retinoic acid receptor (RAR) α1, β2, or γ1 and collagenase promoter-driven CAT reporter constructs, only RAR-γ1 represses basal CAT expression upon RA treatment, while RAR-α1, β2, and γ1 all suppress phorbol-induced CAT expression. Thus, transcriptional regulation of collagenase by RA is mediated by RARs in an RAR-type specific manner. Using mutatlonal and deletional analysis, we find that interaction between elements within 182 bp collagenase promoter plays an …
Evidence For Helical Structures In Poly(1-Olefin Sulfones) By Transmission Electron Microscopy, George C. Ruben, W H. Stockmayer
Evidence For Helical Structures In Poly(1-Olefin Sulfones) By Transmission Electron Microscopy, George C. Ruben, W H. Stockmayer
Dartmouth Scholarship
Transmission electron microscope images were obtained of fractions of poly(1-tetradecene sulfone) and poly(cyclohexene sulfone) cast from very dilute solutions (0.007%, wt/vol) and rapidly freeze-dried on a mica surface. The samples were then vertically platinum-carbon (Pt-C) replicated with 9 +/- 0.3-A Pt-C and held together with 128 A of electron-transparent evaporated carbon. The Pt-C coating enlarges the molecular chain diameters by approximately 5 A, so that a single polysulfone chain has an apparent diameter of 9-12 A in the transmission electron microscope. Poly(1-tetradecene sulfone) forms short helical regions that show irregular helical turns of pitch 7-18 A, two to eight turns …
Mobilization Of Iron Enhances The Iron-Dependent Biochemical Reactivity Of Asbestos In Vitro And Contributes To The Cytotoxicity Of Asbestos In Cultured Cells, Loren Glen Lund
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Asbestos related research began approximately 60 years ago, yet, the mechanism(s) by which asbestos exerts its biological effects is not well understood. The hypothesis upon which this dissertation is based is that mobilization of iron from asbestos enhances the iron-dependent biochemical reactivity of asbestos in vitro and contributes to asbestos-dependent cytotoxicity. The specific aims for this hypothesis were, 1) to determine whether iron was responsible for the biochemical reactivity of asbestos in vitro and asbestos-induced cytotoxicity in cultured cells, and 2) to determine whether mobilization of iron from asbestos enhanced the reactions catalyzed by asbestos in vitro and contributes to …
The Structure Of Residues 7-16 Of The Aɑ-Chain Of Human Fibrinogen Bound To Bovine Thrombin At 2.3 Å Resolution, Philip D. Martin, William Robertson, Dusan Turk, Robert Huber, Wolfram Bode, Brian Fp Edwards
The Structure Of Residues 7-16 Of The Aɑ-Chain Of Human Fibrinogen Bound To Bovine Thrombin At 2.3 Å Resolution, Philip D. Martin, William Robertson, Dusan Turk, Robert Huber, Wolfram Bode, Brian Fp Edwards
Biochemistry and Molecular Biology Faculty Publications
The tetradecapeptide Ac-D-F-L-A-E-G-G-G-V-R-G-P-R-V-OMe, which mimics residues 7f-20f of the A alpha-chain of human fibrinogen, has been co-crystallized with bovine thrombin from ammonium sulfate solutions in space group P2(1) with unit cell dimensions of a = 83.0 A, b = 89.4 A, c = 99.3 A, and beta = 106.6 degrees. Three crystallographically independent complexes were located in the asymmetric unit by molecular replacement using the native bovine thrombin structure as a model. The standard crystallographic R-factor is 0.167 at 2.3-A resolution. Excellent electron density could be traced for the decapeptide, beginning with Asp-7f and ending with Arg-16f in the active …
H-Protein Of The Glycine Decarboxylase Multienzyme Complex: Complementary Dna Encoding The Protein From Arabidopsis Thaliana, David J. Oliver
H-Protein Of The Glycine Decarboxylase Multienzyme Complex: Complementary Dna Encoding The Protein From Arabidopsis Thaliana, David J. Oliver
David J. Oliver
The glycine decarboxylase multienzyme complex is composed of four component enzymes, the 100-kD P-protein (a pyridoxal 5-phosphate-dependent amino acid decarboxylase), the 14-kD H-protein (a lipoamide-containing carrier protein), the 45-kD T-protein (a tetrahydrofolate transferase), and the 59-kD L-protein (lipoamide dehydrogenase) (5). The Iipoamide cofactor of the H-protein carries reaction intermediates between the active sites of the other three component proteins. These proteins form a large molecular weight complex (4) within the matrix ofleaf mitochondria where, together with the enzyme serine hydroxymethyltransferase, it catalyzes the photorespiratory conversion of two molecules of glycine to one each of serine, NH3, and CO2 with the …
Methotrexate As An Alternative To Surgery In Ectopic Pregnancy: A New Role For An Old Drug, M P. Iqbal
Methotrexate As An Alternative To Surgery In Ectopic Pregnancy: A New Role For An Old Drug, M P. Iqbal
Department of Biological & Biomedical Sciences
Abstract is not provided by the author/publisher
Biochemical Analysis Of Human Seminal Plasma, Jamshed Ayub
Biochemical Analysis Of Human Seminal Plasma, Jamshed Ayub
Chemistry & Biochemistry Theses & Dissertations
Presently there is no single biochemical criterion for differentiating between a potentially fertile male and an infertile male. However, for evaluating male fertility the biochemical analysis of seminal plasma is a very convenient and quantitative technique if it is combined with careful clinical examination.
In seminal plasma analysis, fructose and proteins are chemical indicators of seminal vesicle activity, citric acid and acid phosphatase indicate prostate function while glycerolphosphorylcholine indicates epididymis activity. In this research these five indicators are studied and an attempt is made to establish their normal ranges.
This study shows that the data obtained from the seminal plasma …
Estimation And Separation Of N-Acetyl-Beta-D-Glucosaminidase Isoenzymes In Urine, M H. Javed, A N. Hussain, M A. Waqar
Estimation And Separation Of N-Acetyl-Beta-D-Glucosaminidase Isoenzymes In Urine, M H. Javed, A N. Hussain, M A. Waqar
Department of Biological & Biomedical Sciences
N-acetyl-beta-D-glucosaminidase is a lysosomal enzyme made up of two isoenzymes (A and B). It has been used extensively as a marker for kidney damage. However, its estimation in urine has not been standardized. We have established a method for the estimation and separation of NAG isoenzymes by ion-exchange chromatography. In 19 experiments done so far, this method has given reproducible results. The significance of this method is that with a single experiment, one can estimate total as well as individual isoenzyme activity. Furthermore, urine constituents do not appear to interfere in this assay.
Interaction Of Single And Double Stranded Nucleic Acids With Charged Oligopeptides Containing Tryptophan, David Mascotti, T. Lohman
Interaction Of Single And Double Stranded Nucleic Acids With Charged Oligopeptides Containing Tryptophan, David Mascotti, T. Lohman
David P. Mascotti
No abstract provided.
Structural Changes That Accompany The Reduced Catalytic Efficiency Of Two Semisynthetic Ribonuclease Analogs, V. Srini J. De Mel, Philip D. Martin, Marilynn S. Doscher, Brian Fp Edwards
Structural Changes That Accompany The Reduced Catalytic Efficiency Of Two Semisynthetic Ribonuclease Analogs, V. Srini J. De Mel, Philip D. Martin, Marilynn S. Doscher, Brian Fp Edwards
Biochemistry and Molecular Biology Faculty Publications
The structures of two catalytically defective semi-synthetic RNases obtained by replacing aspartic acid 121 with asparagine or alanine have been determined and refined at a resolution of 2.0 A (R = 0.186 and 0.172, respectively). When these structures are compared with the refined 1.8-A structure (R = 0.204) of the fully active aspartic acid-containing enzyme (Martin, P.D., Doscher, M.S., and Edwards, B. F. P. (1987) J. Biol. Chem. 262, 15930-15938), numerous and widespread changes, much greater in number and magnitude than the small structural variations noted previously between the semisynthetic complex and RNase A, are found to have occurred. These …
Lysosomal Enzyme Secretion In Tumor Cells And The Purification And Partial Characterization Of A 67 Kda Cathepsin B-Like Enzyme Secreted By B16 Melanoma Cells, Charles Achkar
Dissertations
No abstract provided.
The Effects Of Ethanol On Cultured Serotonergic Neurons By Denise Kay Lokhorst., Denise Kay Lokhorst
The Effects Of Ethanol On Cultured Serotonergic Neurons By Denise Kay Lokhorst., Denise Kay Lokhorst
Dissertations
No abstract provided.
Improved Recovery Of A Radlolabeled Peptide With An Albumin-Treated Reversed-Phase Hplc Column, David S. Hage, Robert L. Taylor, Pai C. Kao
Improved Recovery Of A Radlolabeled Peptide With An Albumin-Treated Reversed-Phase Hplc Column, David S. Hage, Robert L. Taylor, Pai C. Kao
David Hage Publications
Reversed-phase high-performance liquid chromatography (RP-HPLC) is an important tool in the purification of radiolabeled peptides and proteins for immunoassay. However, for some proteins and peptides it is difficult to achieve reproducible behavior in RP-HPLC because of the low recovery of these compounds. Factors that can be varied to improve recovery include the strength or pH of the mobile phase, the chain length and spacing of groups on the reversed-phase support, and the flow rate or steepness of the elution gradient (1-5). ... In summary, we obtained better recovery and more reproducible chromatographic behavior for labeled 1-34 PTHrP with an albumin-pretreated …
Association Of Tetrapyrrole Intermediates In The Bacteriochlorophyll A Biosynthetic Pathway With The Major Outer-Membrane Porin Protein Of Rhodobacter Capsulatus, David Bollivar
Scholarship
No abstract provided.