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Full-Text Articles in Life Sciences
Detection In Soil Of A Deletion In An Engineered Dna Sequence By Using Dna Probes, Janet K. Jansson, William E. Holben, James M. Tiedje
Detection In Soil Of A Deletion In An Engineered Dna Sequence By Using Dna Probes, Janet K. Jansson, William E. Holben, James M. Tiedje
Biological Sciences Faculty Publications
Two Pseudomonas strains were engineered to contain the nptII gene and plasmid vector sequences in their chromosomes. After incubation of these strains in nonsterile soil, total bacterial DNA was isolated and analyzed by Southern blot hybridization with the nptII gene and the plasmid vector as probes. In addition to the expected bands of hybridization, a new band corresponding to the loss of vector sequences from the chromosome while retaining the nptII gene was observed for one of the strains. The more stressful conditions encountered in soil appeared to increase the frequency of loss of the vector sequences …
Identification And Characterization Of Peptidoglycan-Associated Proteins In Neisseria-Gonorrhoeae, Stuart A. Hill, Ralph C. Judd
Identification And Characterization Of Peptidoglycan-Associated Proteins In Neisseria-Gonorrhoeae, Stuart A. Hill, Ralph C. Judd
Biological Sciences Faculty Publications
The principal proteins associated with Neisseria gonorrhoeae peptidoglycan (PG), as identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, are the following: two proteins at approximately 90 kilodaltons (kDa), single major species at both 60 and 44 kDa, a 34- to 36-kDa protein, and three proteins between 28 and 32 kDa. A protein analogous to Escherichia coli Braun lipoprotein was not detected with gonococcal cell wall preparations. The identity of the PG-associated proteins was confirmed immunologically with antibody generated against purified cell walls. Two types of protein species, dithiothreitol extractable (the majority) and alkylation dependent (primarily the 34- to 36-kDa protein), appeared …
The Structure Of Tumor Necrosis Factor-Α At 2.6 Å Resolution. Implications For Receptor Binding, M. J. Eck, S. R. Sprang
The Structure Of Tumor Necrosis Factor-Α At 2.6 Å Resolution. Implications For Receptor Binding, M. J. Eck, S. R. Sprang
Biological Sciences Faculty Publications
The three-dimensional structure of tumor necrosis factor (TNF-α), a protein hormone secreted by macrophages, has been determined at 2.6 Å resolution by x-ray crystallography. Phases were determined by multiple isomorphous replacement using data collected from five heavy atom derivatives. The multiple isomorphous replacement phases were further improved by real space symmetry averaging, exploiting the non-crystallographic 3-fold symmetry of the TNF-α trimer. An atomic model corresponding to the known amino acid sequence of TNF-α was readily built into the electron density map calculated with these improved phases. The 17,350-dalton monomer forms an elongated, antiparallel β-pleated sheet sandwich with a 'jelly-roll' topology. …
Mapping Regional Forest Evapotranspiration And Photosynthesis By Coupling Satellite Data With Ecosystem Simulation, Steven W. Running, Ramakrishna R. Nemani, David L. Peterson, Larry E. Band, Donald F. Potts, Lars E. Pierce, Michael A. Spanner
Mapping Regional Forest Evapotranspiration And Photosynthesis By Coupling Satellite Data With Ecosystem Simulation, Steven W. Running, Ramakrishna R. Nemani, David L. Peterson, Larry E. Band, Donald F. Potts, Lars E. Pierce, Michael A. Spanner
Forest Management Faculty Publications
Mapping Regional Forest Evapotranspiration and Photosynthesis by Coupling Satellite Data With Ecosystem Simulation
Export And Intercellular Transfer Of Dna Via Membrane Blebs Of Neisseria-Gonorrhoeae, David W. Dorward, Claude F. Garon, Ralph C. Judd
Export And Intercellular Transfer Of Dna Via Membrane Blebs Of Neisseria-Gonorrhoeae, David W. Dorward, Claude F. Garon, Ralph C. Judd
Biological Sciences Faculty Publications
Naturally elaborated membrane bleb material is frequently observed in cultures of Neisseria gonorrhoeae. This material was purified and analyzed for protein, lipopolysaccharide, and nucleic acid content. The electrophoretic protein profiles of two bleb-rich fractions, called BI and BII, were distinct, with only BII containing lipopolysaccharide and outer membrane proteins I and III. Both fractions contained RNA, circular DNA, and linear DNA. Exogenous pancreatic DNase I appeared to hydrolyze all bleb-associated DNA in fraction BI and the linear DNA in fraction BII. The circular DNA molecules associated with fraction BII resisted digestion. Electron microscopy of the bleb fractions verified their DNA …
Protein I: Structure, Function, And Genetics, Ralph C. Judd
Protein I: Structure, Function, And Genetics, Ralph C. Judd
Biological Sciences Faculty Publications
No abstract provided.
Identification Of A Variant Form Of The Human Estrogen Receptor With An Amino Acid Replacement, T. Garcia, M. Sanchez, J. L. Cox, P. A. Shaw, J. B. A. Ross, S. Lehrer, B. Schachter
Identification Of A Variant Form Of The Human Estrogen Receptor With An Amino Acid Replacement, T. Garcia, M. Sanchez, J. L. Cox, P. A. Shaw, J. B. A. Ross, S. Lehrer, B. Schachter
Chemistry and Biochemistry Faculty Publications
Estrogen receptor (ER) is a ligand-dependent transcriptional regulator. ER protein has several functionally distinct regions, including a DNA-binding domain in its midportion, a ligand binding domain in the carboxy terminal region, and at least one domain, in the amino terminal region, that influences the magnitude of transcriptional enhancement (1,2). We now report the sequence of a frequently occurring ER genetic variant that carries two point mutations in the region of the gene encoding the amino terminal domain of the protein.