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Articles 1 - 13 of 13
Full-Text Articles in Life Sciences
Novel Microbiological Medium Developed For The Isolation Of Bacteria Associated With Estuarine Anemones, Parker K. Lund, Catalina Cuellar-Gempeler
Novel Microbiological Medium Developed For The Isolation Of Bacteria Associated With Estuarine Anemones, Parker K. Lund, Catalina Cuellar-Gempeler
IdeaFest: Interdisciplinary Journal of Creative Works and Research from Cal Poly Humboldt
Out of the nearly one trillion species of microbiota estimated to inhabit Earth only ten thousand have been cultured in the laboratory. Culturing continues to play a vital role in determining the physiology and ecologic function of individual bacteria in microbial communities and for microbes associated with host organisms one of the major challenges is developing microbiological media that mimics the bacteria’s natural environment enough to promote growth. Here, we target bacteria associated with the estuary anemones Diadumene lineata and Metridium senile by developing a novel medium that uses anemone tissue as the sole source of nutrients. We further measured …
Exploring Representation In Microbiology Introductory Courses Can Encourage A More Inclusive And Inspiring Environment For Students And Instructors, Jill A. Mikucki, Elizabeth Fozo
Exploring Representation In Microbiology Introductory Courses Can Encourage A More Inclusive And Inspiring Environment For Students And Instructors, Jill A. Mikucki, Elizabeth Fozo
Feminist Pedagogy
Microbiology has a relatively brief history where significant discoveries are often linked with major events in human history - from disease outbreak to industrialization to climate change. The founders of key microbiological principles span across continents, genders, ethnicities, and socioeconomic status. However, the portrait described in many introductory textbooks center around a lone, typically white male scientist. Such narratives not only are misleading regarding the development of key principles in microbiology but can also reinforce inappropriate stereotypes as to whom belongs in microbiology. In our introductory microbiology course, we designed group work for Zoom break-out rooms to help engage students …
Contrast Of Smokeless Tobacco And It's Microbiological Footprint In Users And Non-Users, Monayjia Brown, Katie Robbins, Elyse Mckinney, Sydney Snell
Contrast Of Smokeless Tobacco And It's Microbiological Footprint In Users And Non-Users, Monayjia Brown, Katie Robbins, Elyse Mckinney, Sydney Snell
Humboldt Journal of Microbiology
Smokeless tobacco products such as vapes have gained significant popularity over the last 20 years (7), and it is clear that the effects of these products are primarily unknown. This study examines how the microbial profiles found in smokeless tobacco products, such as vapes, can be used in forensic science. By linking these profiles to their owners, investigators can identify suspects in cases where traditional evidence is lacking. This information could provide valuable assistance in tracking victims and matching perpetrators or victims to crime scenes. Forensic science involves any evidence found at a crime scene that is examined and analyzed …
Food Safety Practices Among Food Carts In -North Lebanon, Hanin Hassan, Nada El Darra, Alissar Al Khatib
Food Safety Practices Among Food Carts In -North Lebanon, Hanin Hassan, Nada El Darra, Alissar Al Khatib
BAU Journal - Health and Wellbeing
Street food carts serve different types of popular and traditional foods; it is a common economic sector worldwide. In North Lebanon, food carts are mobile or centered in specific places serving common and well known RTE meals. In addition, the increasing incidence of foodborne illnesses associated with street foods sheds light on the importance of inspecting the practices of street vendors. Therefore, the aim of this study is to assess the food safety in 30 food carts using an observational checklist and to perform microbiological analysis for the detection of foodborne pathogens namely; yeast, mold, Listeria monocytogenes, Salmonella, Enterohemorrhagic E.coli, …
Survival Of Enterococcus FaeciumIn Turkey Litter Under Different Temperature And Moisture Combinations, Steven G. Mcbride, Benjamin Holland, Pradeep Vasudevan, Joanna B. Mott
Survival Of Enterococcus FaeciumIn Turkey Litter Under Different Temperature And Moisture Combinations, Steven G. Mcbride, Benjamin Holland, Pradeep Vasudevan, Joanna B. Mott
Virginia Journal of Science
Untreated poultry litter introduces a substantial load of fecal pathogens to the environment, impacting agriculture, public health and ecosystem function. There is substantial evidence that temperature and moisture are the primary drivers of fecal bacteria survival across ecosystems. However, both temperature and moisture effects have been shown to be modulated by the matrix in which the fecal bacteria are living. This context dependence highlights the importance of understanding fecal bacteria survival in a variety of matrices in order to implement effective waste management plans. In this study, we determined the survival patterns of Enterococcus faecium in post-use turkey litter under …
A Cultured Learning Environment: Implementing A Problem- And Service-Based Microbiology Capstone Course To Assess Process- And Skill-Based Learning Objectives, Rachel M. Watson, John D. Willford, Mariel A. Pfeifer
A Cultured Learning Environment: Implementing A Problem- And Service-Based Microbiology Capstone Course To Assess Process- And Skill-Based Learning Objectives, Rachel M. Watson, John D. Willford, Mariel A. Pfeifer
Interdisciplinary Journal of Problem-Based Learning
In this study, a problem-based capstone course was designed to assess the University of Wyoming Microbiology Program’s skill-based and process-based student learning objectives. Students partnered with a local farm, a community garden, and a free downtown clinic in order to conceptualize, propose, perform, and present studies addressing problems experienced by these partners. Instructor assessments enabled understanding of student competencies, and according to external subject matter experts students demonstrated mastery of all learning objectives on the final research presentation. Community partners were completely satisfied with the students’ solutions, professionalism, and communication. Instructional diagnosis and student course evaluations showed satisfaction, engagement, and …
Genetic Diversity Of Mycobacteriophages And The Unique Abilities Of Cluster K, Shea A. Morris
Genetic Diversity Of Mycobacteriophages And The Unique Abilities Of Cluster K, Shea A. Morris
The Corinthian
Mycobacteriophage are a diverse set of viruses that infect a broad host range of mycobacterium. Genome analysis of this phage group reveals its diversity and provides evidence that they have experienced substantial horizontal gene transfer Collectively they have a large range of host bacteria they can infect, although each viral type may have a limited host range. However, this range is easily expanded and constantly changing in nature by mutation within and between virus types. These phages can lytically destroy their host mycobacteria, and it is this viral ability that have many mycobacteriophage researchers excited about the potential that phage …
Nisin Resistance Of Bacillus Cerus: Preparation Of Nisin, Peter Weber
Nisin Resistance Of Bacillus Cerus: Preparation Of Nisin, Peter Weber
Journal of Undergraduate Research at Minnesota State University, Mankato
Nisin is a peptide that is made by the bacterium Lactococcus lactis. Nisin is a small molecule that kills gram positive bacteria by binding to their membrane and by disrupting the proton motive force. When food is processed it is heated to kill bacteria, but some bacteria still survive. Adding nisin to the food provides a second barrier for the growth of the bacteria. Purified nisin has become quite expensive in the current marketplace. Therefore this study is directed at producing nisin and purifying it. L. lactis was grown in five different media (BHI, BHI + 1% glucose, BHI + …
The Innate Immune Response In Eisenia Fetida To Microbial Challenges, Aaron Schindler
The Innate Immune Response In Eisenia Fetida To Microbial Challenges, Aaron Schindler
Journal of Undergraduate Research at Minnesota State University, Mankato
The common earthworm, Eisenia fetida, exhibits a rudimentary immune system. The earthworm needs cellular and chemical responses against a constant microbial exposure from its natural environment. Some cellular and chemical responses are found in the coelomic fluid and have been shown to demonstrate anti-microbial characteristics. This project uses microscopy and modified staining techniques to differentiate and categorize the cellular components found in the coelomic fluid. Following a microbial challenge by Klebsiella pneumoniae, an inflammatory response was initiated. Six groups of earthworms were injected with 0.05 ml of 1.0 x 106 cfu /ml K. pneumoniae on day one and tested over …
Mycological Study For A Management Plan Of A Neotropical Show Cave (Brazil), Erika Linzi Silva Taylor, Maria Aparecida De Resende Stoianoff, Rodrigo Lopes Ferreira
Mycological Study For A Management Plan Of A Neotropical Show Cave (Brazil), Erika Linzi Silva Taylor, Maria Aparecida De Resende Stoianoff, Rodrigo Lopes Ferreira
International Journal of Speleology
Caves are stable environments with characteristics favoring the development of microorganisms. The allocthonous input of organic matter and microbes into the warm Neotropical caves may favor the development of filamentous fungi, including pathogenic species. Histoplasma capsulatum is a pathogenic species commonly found in caves and associated with bat and bird guano. Many Brazilian caves have been historically visited due to scenic and religious tourism. The objective of this study was to perform a microbiology study for a management plan of a show cave in Brazil, focusing on the presence and distribution of pathogenic and opportunistic fungi in the cave. Statistics …
Defining Anuran Malformations In The Context Of A Developmental Problem, Carol U. Meteyer, Rebecca A. Cole, Kathryn A. Converse, Douglas E. Docherty, Mark Wolcott, Judy C. Helgen, Richard Levey, Laura Eaton-Poole, James G. Burkhart
Defining Anuran Malformations In The Context Of A Developmental Problem, Carol U. Meteyer, Rebecca A. Cole, Kathryn A. Converse, Douglas E. Docherty, Mark Wolcott, Judy C. Helgen, Richard Levey, Laura Eaton-Poole, James G. Burkhart
Journal of the Iowa Academy of Science: JIAS
This paper summarizes terminology and general concepts involved in animal development for the purpose of providing background for the study and understanding of frog malformations. The results of our radiographic investigation of rear limb malformations in Rana pipiens provide evidence that frog malformations are the product of early developmental errors. Although bacteria, parasites and viruses were identified in these metamorphosed frogs, the relevant window to look for the teratogenic affect of these agents is in the early tadpole stage during limb development. As a result, our microbiological findings must be regarded as inconclusive relative to determining their contribution to malformations …
Microbiological Immunocytochemistry: A Review Of Current Trends And Applications, Julian E. Beesley
Microbiological Immunocytochemistry: A Review Of Current Trends And Applications, Julian E. Beesley
Scanning Microscopy
It has been considered worthwhile to update previous reviews of microbiological immunocytochemistry in order to identify areas of current importance in this continually expanding area of research. Publications in virology, bacteriology and protozoology indicate a continued interest in immunocytochemistry. Deployment of colloidal gold techniques is almost universal in these applications. The post-embedding technique was the most widely applied technique although a few studies employed the immunonegative stain, pre-embedding and immunoreplica techniques, thereby reflecting the use of colloidal gold in all other areas of the biological sciences.
Bioapplication Of Colloidal Gold In Microbiological Immunocytochemistry, Julian E. Beesley
Bioapplication Of Colloidal Gold In Microbiological Immunocytochemistry, Julian E. Beesley
Scanning Microscopy
Microbiological organisms are an ubiquitous group of animals encompassing bacteria, viruses, protozoa, algae and fungi. They are adapted for survival in many diverse habitats and exert a profound effect on man and his environment. Colloidal gold electron immunocytochemistry is a useful technique for studying these organisms and may be applied in several ways. The post-embedding technique is used to detect internal antigens, whilst the pre-embedding technique is employed for the detection of external antigens. In contrast the immuno-negative stain technique is applied to detect antigens on structures such as viruses or bacterial pili which may be dried down onto an …