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Articles 1 - 9 of 9
Full-Text Articles in Life Sciences
Establishing And Validating Methods To Assess The Influence Of Exercise On Circulating Exosome Profiles In Sedentary Older Adults, Heather Quiriarte
Establishing And Validating Methods To Assess The Influence Of Exercise On Circulating Exosome Profiles In Sedentary Older Adults, Heather Quiriarte
LSU Master's Theses
Aging is associated with progressing cellular structural and functional changes, and sedentary behavior can exacerbate these hallmarks of aging. Routine exercise is known to have beneficial effects on physiological systems, but the exact molecular mechanisms behind the health benefits are unclear. Emerging evidence suggests extracellular vesicles called exosomes are involved in the intercommunication between cells and organs following exercise by delivering their cargo such as proteins and miRNAs. miRNA profiles in older adults following acute and chronic exercise need further exploration. Specifically, the effects of regular low-intensity physical activity (LPA) breaks in sedentary time on proteins and miRNA packaged in …
The Distinctive Regulatory Mechanisms Of Bacterial Acetyl-Coa Carboxylase, Alexandra Leigh Evans
The Distinctive Regulatory Mechanisms Of Bacterial Acetyl-Coa Carboxylase, Alexandra Leigh Evans
LSU Doctoral Dissertations
Metabolic Regulation is a complex system used to control cellular metabolism in response to conditions in the cell’s environment. For most enzymes, the cell can rely upon a minimal amount of regulation; however, critical enzymes, such as acetyl-CoA carboxylase, must be regulated at multiple levels. Acetyl-CoA carboxylase catalyzes the first committed step in fatty acid synthesis. In bacteria, acetyl-CoA carboxylase forms a complex of three subunits–biotin carboxylase, biotin carboxyl carrier protein, and carboxyltransferase–which catalyze the carboxylation of acetyl-CoA to form malonyl-CoA via two half-reactions. In the first half-reaction, biotin covalently linked to biotin carboxyl carrier protein is carboxylated by biotin …
Functional And Structural Analyses Of A Bacterial Antifreeze Protein, Chen Wang
Functional And Structural Analyses Of A Bacterial Antifreeze Protein, Chen Wang
LSU Doctoral Dissertations
Antifreeze proteins (AFPs) are a class of ice binding proteins (IBPs) that are expressed by different cold-adapted organisms to increase their freezing tolerance. AFPs have two major properties: thermal hysteresis and ice recrystallization inhibition. Here we report the functional and structural analyses of a bacterial AFP, IBPv. IBPv was originally secreted by a bacterium recovered from a deep glacial ice core drilled at Vostok Station, Antarctica. Our study showed that the recombinant protein rIBPv exhibited a thermal hysteresis of 2°C at concentrations higher than 50 µM, effectively inhibited ice recrystallization, and enhanced bacterial viability during freeze-thaw cycling. Circular dichroism scans …
Fibroblast Growth Factor 21 Is A Novel Protein Sensor In Pregnancy, Elizabeth Frost Sutton
Fibroblast Growth Factor 21 Is A Novel Protein Sensor In Pregnancy, Elizabeth Frost Sutton
LSU Doctoral Dissertations
The twenty-first century has experienced a shift in cause of death worldwide from communicable diseases to noncommunicable diseases. Interestingly, many of these implicated chronic diseases, such as cancer, diabetes, and cardiovascular disease, have been shown to be programmed in the womb. As first posited by the Barker Hypothesis, adverse exposures in utero can increase an individual’s risk for chronic disease later in life. Therefore, pregnancy is an opportune time for intervention to improve the health of future generations. Studies of exposures known to negatively impact infant health, e.g. states of overnutrition (obesity, diabetes, excess gestational weight gain) and undernutrition (starvation, …
Discovering Potential Protein, Carbohydrate, And Lipid Based Food Ingredients In A Co-Culture Of Microalgae, Chelsea Tyus
Discovering Potential Protein, Carbohydrate, And Lipid Based Food Ingredients In A Co-Culture Of Microalgae, Chelsea Tyus
LSU Doctoral Dissertations
Louisiana Native Co-Culture of Microalgae (Chlorella Vulgaris L. and Cyanobacteria Leptolyngbya sp.) (CCA) was studied. CCA is a viable coculture for further investigation as a food component. This research characterized the proteins, carbohydrates, and lipids present in CCA. Algae cultivation parameters were controlled and analyzed. Treatment (Trt) 1 was CCA grown in cultures exposed to average scalar irradiance (ASI) of 1041 ± 269.18 μmol m-2 s-1 PAR and trt 2 was CCA grown in cultures exposed to ASI of 430 ± 96.03 μmol m-2 s-1 PAR. The trt (irradiance exposure) had the desired response on CCA species ratio Trt …
Reduced Protein For Late-Lactation Dairy Cows, Andre De Barros Duarte Pereira
Reduced Protein For Late-Lactation Dairy Cows, Andre De Barros Duarte Pereira
LSU Master's Theses
Excess protein in dairy cattle diets unnecessarily increases the cost of production and may contribute to environmental pollution. The objective of this research was to evaluate the effect of feeding dairy cows with two levels of dietary protein on animal performance and manure characteristics. Two experiments were carried out with 24 lactating dairy cows each. Experiment 1 was corn silage based and had a control TMR (HP1) estimated to contain 16.5% CP with SBM and treatment TMR (LP1; 13.5% CP) using DDGS and rumen protected Lys and Met. Experiment 2 was ryegrass haylage based and had a control TMR (HP2) …
Effect Of Milk Replacer Composition On Growth And Rumen Development Of Neonatal Holstein Calves, Anthony Joseph Bridges
Effect Of Milk Replacer Composition On Growth And Rumen Development Of Neonatal Holstein Calves, Anthony Joseph Bridges
LSU Master's Theses
A study was conducted to determine effects of differing fat and protein levels in commercial milk replacers on the growth performance and rumen development of neonatal Holstein calves. Fifty-three calves (23 male; 30 female) were assigned to one of three dietary treatments which included 3 milk replacers (20/20; 25/15; 28/20) fed once per day at 10% of birth weight through weaning. Calves were offered a 20% CP calf starter and water free choice beginning on day 5. All calves were weaned on day 42. Body weights were measured at birth and weekly through weaning. Additionally, hip height, wither height, and …
Development, Evaluation And Characterization Of Protein-Isoflavone Enriched Soymilk, Janette Ethel-Pessi Saidu
Development, Evaluation And Characterization Of Protein-Isoflavone Enriched Soymilk, Janette Ethel-Pessi Saidu
LSU Doctoral Dissertations
Adequate provision of soy isoflavone and protein in soybean products is essential in meeting the soy health claim and the promotion of a healthy product. Commercial soymilks contain inadequate amounts of protein and isoflavone to meet the FDA recommended 25g and 40-50mg/day level, respectively, in 1-2 servings. This research study set out to investigate the possibility of producing a protein-isoflavone enriched (PIE) soymilk by incorporating soy germ in soymilk to provide the recommended FDA level in 1-2 servings. Three PIE soymilks containing 25 (SG25), 30 (SG30), and 35% (SG35) germ were prepared and compared to 100% whole soybean milk (WSB-control) …
Evaluation Of Lutein And Protein In Ozone Treated Corn, Yu Wang
Evaluation Of Lutein And Protein In Ozone Treated Corn, Yu Wang
LSU Master's Theses
The present study was conducted to determine the influence of an ozonation process on lutein and protein in clean and contaminated corns. This study aimed (1) to determine the levels of antimutagenic compounds to aflatoxin in corn before and after ozonation; (2) to verify the antimutagenic potential of these compounds against aflatoxin using the Ames test; and (3) to evaluate proteins in the corn before and after ozonation. HPLC was used to separate and identify the lutein in corn. Nitrogen analysis and SDS-PAGE were used to analyze protein content and molecular weights in corn. Clean ozone-treated corn had a total …