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Full-Text Articles in Life Sciences

Identification Of Uncommon Antibiotic-Producing Illinois Soil Isolates, Lesly Muniz, Dr. Lori Scott Jan 2020

Identification Of Uncommon Antibiotic-Producing Illinois Soil Isolates, Lesly Muniz, Dr. Lori Scott

Identifying and Characterizing Novel Antibiotic Producing Microbes From the Soil

This project is a collaboration with the Tiny Earth Project Initiative (TEPI), which is a global network of educators and students focused on student sourcing antibiotic discovery from the soil. We researched tester strains B. subtilis and E. coli from the soil isolates obtained. We further verified if the isolates were common antibiotic bacteria. Unfortunately, this project heavily relied on biochemical tests, colony morphology, and Gram stains to reject or fail to reject our hypothesis. Our goal was to discover new antibiotic-producing bacteria that could be beneficial in combating ESKAPE strains. A proper PCR and DNA extraction would be required …


Isolated Antibiotic Producing Bacteria In Local Soil Samples Determined To Be Bacillus, Cassidy Potter, Dr. Lori Scott Jan 2020

Isolated Antibiotic Producing Bacteria In Local Soil Samples Determined To Be Bacillus, Cassidy Potter, Dr. Lori Scott

Identifying and Characterizing Novel Antibiotic Producing Microbes From the Soil

Nosocomial pathogens are multi-drug resistant to antibiotics that fight bacterial infections posing danger to the public health, the most dangerous of them being the ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.). This project is a collabortaion with the TIny Earth Project Initiative (TEPI), which is a global network of educators and students focused on studentsourcing antibiotic discovery from the soil. TEPI allows student-led research on local soil samples from Bettendorf, IA to discover potential novel antibiotic producing bacteria that could potentially treat ESKAPE pathogens and reduce public health risk. Two soil isolates …


Isolating Antibiotic-Producing Bacteria From Soil, Michelle Santiago, Dr. Lori Scott Jan 2020

Isolating Antibiotic-Producing Bacteria From Soil, Michelle Santiago, Dr. Lori Scott

Identifying and Characterizing Novel Antibiotic Producing Microbes From the Soil

As the antibiotic crisis becomes stronger, we are in need of finding more novel antibiotics. This project is a collaboration with the Tiny Earth Project Initiative (TEPI), which is a global network of educators and students focused on student sourcing antibiotic discovery from soil. The soil isolates were determined to be part of the Bacillus and Pseudomonas genus.


Putative Antibiotic Producer: A Pseudomonas Genus With Antibiotic Properties, John Tworek, Dr. Lori Scott Jan 2020

Putative Antibiotic Producer: A Pseudomonas Genus With Antibiotic Properties, John Tworek, Dr. Lori Scott

Identifying and Characterizing Novel Antibiotic Producing Microbes From the Soil

There is a series antibiotic crisis in the world with catastrophic consequences if action is not take. Many diseases caused by bacteria are becoming untreatable because of the amount of pathogens resistant to the effect of antibiotics. The most prolific bacteria are the ESKAPE bacteria. They are nonsocomial pathogens that exhibit multi-drug resistance and virulence. My project will be working alongside the Tiny Earth Project (TEPI) to educate the public about the antibiotic crisis as well as obtaining a soil sample to possible discover new antibiotics. The DNA sequencing data retrieved from soil isolates against the two ESKAPE tester strains …


Screening For Antibiotic-Producers In Soil From A Garden, Long Tran, Dr. Lori Scott Jan 2020

Screening For Antibiotic-Producers In Soil From A Garden, Long Tran, Dr. Lori Scott

Identifying and Characterizing Novel Antibiotic Producing Microbes From the Soil

Multidrug-resistant pathogens are the leading cause of nosocomial infection, which killed more than 30,000 people in the United States every year. Among these, ESKAPE strains bugs, which comprise six highly drug-resistant bacteria, pose the greatest challenge to the healthcare system. In order to fight the antibiotic-resistant crises, novel antibiotic-producers must be discovered. This project is a collaboration with the Tiny Earth Project Initiative (TEPI), which is a global network of educators and students focused on student sourcing antibiotic discovery from the soil. Pseudomonas was revealed to produce a zone of inhibition against Bacillus subtilis on LB media. The next step …


Unknown Soil Isolates From Urban Park As Antibiotic Producers, Sydney Parra, Dr. Lori Scott Jan 2020

Unknown Soil Isolates From Urban Park As Antibiotic Producers, Sydney Parra, Dr. Lori Scott

Identifying and Characterizing Novel Antibiotic Producing Microbes From the Soil

Antibiotics play an important role in combating bacterial infections. Recently, antibiotic resistance has increased which has pushed scientist to find new and effective antibiotic drugs. Students conducted research on potential antibiotic producers from soil samples in the Quad Cities region. The purpose of the study was to find novel antibiotic producers from bacteria in soil and test soil isolates against two strains of bacteria, B. subtilis and E. coli. Two unknown soil isolate strains were found to be potential antibiotic producers against B. subtilis. Future studies are needed to conduct gene sequencing on these two …


Pseudomonas And Bacillus As Potential Sources Of Novel Antibiotics, Cory Jayne, Dr. Lori Scott Jan 2020

Pseudomonas And Bacillus As Potential Sources Of Novel Antibiotics, Cory Jayne, Dr. Lori Scott

Identifying and Characterizing Novel Antibiotic Producing Microbes From the Soil

With an increase in antibiotic resistance within the medical field the need for novel antibiotics is evident. Through collaboration with the Tiny Earth Project Initiative, we hope to find novel antibiotics through bacteria found in soil. We were able to successfully isolate two microbes that showed broad spectrum antibiotics against both Staphylococcus epidermis and Bacillus subtilis. Through further exploration and collaboration with the Tiny Earth Project Initiative, we might be able to discover novel antibiotics.


Antibiotic Producing Microbes Found In Soil, Rediat Ephrem, Dr. Lori Scott Jan 2020

Antibiotic Producing Microbes Found In Soil, Rediat Ephrem, Dr. Lori Scott

Identifying and Characterizing Novel Antibiotic Producing Microbes From the Soil

The antibiotic crisis is a growing concern as more and more diseases are becoming untreatable due to antibiotic resistance. Of all antibiotic resistant bacteria, the ESKAPE strains are the most dangerous because of their association with higher mortality rates and multidrug resistance. In this study, ESKAPE-like strains will be studied as they are similar to the ESKAPE strains and provide comparable data in a safe manner. Soil samples are evaluated to test the presence of any microbes that may be antibiotic producers. This project is in collaboration with the Tiny Earth Project Initiative (TEPI), which is a global network of …


Pseudomonas And Bacillus Soil Isolates Produce Antibiotics, Chelsea Brandt, Dr. Lori Scott Jan 2020

Pseudomonas And Bacillus Soil Isolates Produce Antibiotics, Chelsea Brandt, Dr. Lori Scott

Identifying and Characterizing Novel Antibiotic Producing Microbes From the Soil

The recent emergence of antibiotic resistance bacterial strains presents a significant challenge and threat to human healthcare. While new methods of treatment such as bacteriophage therapy and combinations of existing antibiotics are being researched, the human population is in dire need of new antibiotics to replace those that are ineffective. This research addresses this need by identifying antibiotic producing bacteria in a soil sample from Davenport, IA. This project is a collaboration with the Tiny Earth Project Initiative (TEPI), which is a global network of educators and students focused on studentsourcing antibiotic discovery from soil. Microbiology lab techniques and 16S …


Identification Of Antibiotic Producing Soil Bacteria Against Bacillus Subtilis, Morgan Brockhouse, Dr. Lori Scott Jan 2020

Identification Of Antibiotic Producing Soil Bacteria Against Bacillus Subtilis, Morgan Brockhouse, Dr. Lori Scott

Identifying and Characterizing Novel Antibiotic Producing Microbes From the Soil

This project is a collaboration with the Tiny Earth Project Initiative (TEPI), which is a global network of educators and students focused on student-sourcing antibiotic discovery from soil. Individual strains of soil bacteria were isolated and produced antibiotic against Bacillus subtilis. Two of these samples were sequenced using the 16S rRNA gene to reveal they are very closely related to the genus Pseudomonas.


Impacts From The Use Of Antibiotics In Livestock: Methods Of Transmission Of Antibiotic Resistance From Livestock To Humans, Kristin M. Walden May 2016

Impacts From The Use Of Antibiotics In Livestock: Methods Of Transmission Of Antibiotic Resistance From Livestock To Humans, Kristin M. Walden

Biology: Student Scholarship & Creative Works

Antibiotic use in livestock production has been around since the 1950s. Antibiotic feed is used in livestock and other meat producing animals for three reasons: illness prevention, illness treatment, and growth promotion. Unfortunately, since the time that antibiotics were first invented, antibiotic resistant bacteria have become a threat to public health. There are many studies showing methods of transmission of antibiotic resistance from livestock to humans. Antibiotic resistance can spread from livestock to soil, water, insects, and food, which ultimately comes into contact with humans. A proposed study to measure antibiotic resistance when eliminating antibiotic feed will provide a hypothesis …