Life Sciences Commons^™

Molecular Characterization Of Fecundity-Related Gene Regions In Some Of Türkiye’S Native Sheep Breeds, Ramazan Aymaz, Fulya Özdi̇l, Yalçın Yaman

Turkish Journal of Veterinary & Animal Sciences

In this study, the BMP15 and GDF9 gene regions were examined through DNA sequencing in some native sheep breeds of Türkiye (Kıvırcık, Karacabey Merino, Chios, Gökçeada, Çine Çaparı, Awassi, and Karakacan). In the BMP15 exon 2 region, two single nucleotide polymorphisms (SNPs) were identified: one synonymous SNP, P248P (proline/proline), and one nonsynonymous SNP, L251P (leucine/proline), resulting in a missense mutation. In the GDF9 exon 1 region, only a nonsynonymous SNP, R87H (histidine/ arginine), was found. In the GDF9 exon 2 region, four SNPs were identified: two nonsynonymous SNPs, E241K (glutamate/lysine) and V332I (valine/isoleucine), and two synonymous SNPs, L159L (leucine/leucine) and …

The Effect Of Saccharomyces Cerevisiae As A Probiotic On The Nutrient Degradability Of Some Commonly Feedstuffs Used In Turkey, Muslimo Abdullahi Sheikh Hassan, Mehmet Aki̇f Karsli

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to evaluate the effects of Saccharomyces cerevisiae as a probiotic on in situ ruminal DM, OM, CP and starch degradability values in some energy and protein sources commonly used in ruminant nutrition. In this study, the in situ degradation kinetics and fractions of dry matter, organic matter, crude protein, and starch of 6 different feedstuffs (barley, wheat, corn, sunflower seed meal, cottonseed meal and soybean meal) commonly used in animal nutrition were determined. Three ruminally cannulated Bafra sheep were used in the experiment. The experiment was designed as carryover experimental design with two periods. …

Anthelmintic Resistance In Sheep Across Ireland And The Uk: A Literature Review Of The In Vivo Versus In Vitro Methods, Megan Tumulty

SURE Journal: Science Undergraduate Research Experience Journal

Background: The animal welfare and production consequences of gastrointestinal nematode infections, namely Nematodirus battus, on sheep are compelling. The control of gastrointestinal infections has been heavily reliant on the administration of anthelmintics since their introduction into mainstream use in 1960. However, their frequent and often excessive use has resulted in anthelmintic resistance being reported extensively in several gastrointestinal nematode species. The prevalence of anthelmintic resistance as well as the increase in the number of cases of multiple-drug resistance in nematode populations now poses substantial hindrance on the viability of sheep production systems. However, despite the threat N. battus poses …

Anatomical And Histological Structures Of Eye And Lacrimal Gland In Norduz Andmorkaraman Sheep, Semi̇ne Dalga, Serap İlhan Aksu, Kadi̇r Aslan, Turgay Deprem, Reşi̇t Uğran

Turkish Journal of Veterinary & Animal Sciences

The current study was conducted to determine the morphological, morphometric, and histological characteristics of bulbus oculi and lacrimal gland in sheep. For this purpose, 28 pairs (n = 7 males and n = 7 females) of bulbus oculi from Morkaraman and (n = 7 males and n = 7 females) Norduz sheep were analyzed. The bulbus oculi in the skulls were dissected from the orbit and removed. The morphometric data such as distance between endpoints of the dissected bulbus oculi in temporal, nasal, and dorsal directions, the length between endpoints of the pupil in dorsal and ventral directions, and as …

Some Hematological Parameters And Enzyme Histochemistry Of Peripheral Bloodleukocytes In Kivircik Sheep, Mehmet Faruk Aydin, İhsan Kisadere

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to determine some hematological parameters (RBC, WBC, HCT, HGB, MCH, MCHC, MCV, and PLT), the presence and percentages of ANAE and AcP-ase positivities in leukocytes and the differential leukocyte count in Kivircik sheep. Blood samples were taken from the jugular vein of 40 (rams = 20; ewes= 20) healthy, 1-year-old sheep. MCH and MCHC values were found statistically higher in rams than ewes (p < 0.05). Mean percentages of ANAE-positive PBL were defined as 74.16 ± 11.94% in ewes and 70.33 ± 8.39% in rams. It was not determined any significant changes according to sex. On the other hand, the percentages of ACP were determined as 67.12 ± 6.54% in ewes and 66.71 ± 6.58% in rams. It was not found any significant differences according to sex. For ANAE enzyme activity, lymphocytes, monocytes, neutrophils, and eosinophilic granulocytes showed a positive reaction. For ACP enzyme activity, although lymphocytes presented positivity, monocytes, neutrophils, eosinophils were negative. The ratio of lymphocyte, monocyte, neutrophile, eosinophile, and basophile of Kivircik ewes and rams were found as 64.35 ± 5.74%, 60.64 ± 8.85%; 3.41 ± 1.27%, 3.82 ± 1.42%; 27.58 ± 5.46%, 30.94 ± 8.71%; 2.41 ± 0.93%, 2.88 ± 1.45%; 1.76 ± 0.75%, 1.29 ± 0.58%, respectively. The difference between mean values was not found significant according to sex (p > 0.05). In conclusion, our study showed that enzyme histochemical methods can be used for identifying peripheral blood cells and can provide facilitates for early diagnosis of immune system-related disorders …

Relationships Between Famacha Scores And Some Blood Values In Sheep And Goats, Özcan Şahi̇n, İbrahi̇m Ayteki̇n, Sai̇m Boztepe, İsmai̇l Keski̇n, Ali̇ Karabacak, Yasi̇n Altay, Mervan Bayraktar

Turkish Journal of Veterinary & Animal Sciences

In this study, Akkaraman sheep and Hair goats raised extensively on the same farm were scored with the FAMACHA card. Score 1 was not present in both sheep and goats, whereas scores 5 and 2 were not detected in sheep and goats, respectively. While the number of animals with scores of 2, 3, and 4 was 9, 46, and 55 in sheep, the number of animals with 3, 4, and 5 scores in goats was 6, 55, and 41, respectively. In the study, the differences in the amount of RBC, Hct, and Hb in goats according to these score groups …

Cranial Cruciate Ligament Desmotomies In Sheep Resulting In Peroneus Tertius Injury, Peter J. Welsh, Crystal G. Collier, Holly M. Clement, Michael N. Vakula, Jeffrey B. Mason

Animal, Dairy, and Veterinary Science Student Research

Surgical destabilization of the stifle joint via cranial cruciate ligament desmotomy (CCLD) is a routine procedure for the study of osteoarthritis (OA). Traditionally performed in rats, rabbits, cats, and dogs, CCLD in sheep provides an opportunity to study the pathology and treatment of joint instability in a species whose stifle better represents the equivalent human femorotibial joint. The surgical approaches for CCLD in sheep are variable and can result in inconsistent outcomes. Eight sheep underwent CCLD for use in a gene therapy study. We report this case in which six of the eight sheep were clinically diagnosed by pathognomonic signs …

A Research On Babesia And Theileria Species In Sheep And Goats Of Kırıkkale Provincethrough Molecular Methods, Sami̇ Gökpinar, Aycan Nuri̇ye Gazyağci, Meral Aydeni̇zöz, Ufuk Kaya

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to determine presence and prevalence of Babesia and Theileria species in the sheep and goats of stock farming in the province of Kırıkkale, which is in Central Anatolia Region of Turkey through microscopic examination and RLB method. Venous blood samples of 3 mL were collected from vena jugularis of 300 sheep and 100 goats into tubes containing EDTA. Blood smears were prepared from samples; and stained by Giemsa and examined for Theileria and Babesia under a light microscope. Genomic DNAs were extracted from blood samples with EDTA through suitable methods; a region with …

Ocular Ultrasonography And Echobiometry In Sheep, Hakim Athar, Jalal Udin Parrah, Nida Handoo, Abdul Qayoom Mir, Mehraj Udin Dar, Beenish Qureshi

Turkish Journal of Veterinary & Animal Sciences

Ocular sonography and echobiometry was performed in local nondescript sheep (N = 36) of different age group and in adult corriedale (N = 60), crossbred Fec B (N = 48), and Poll Dorset cross breed sheep (N = 48). Cornea, anterior, posterior lens capsule, and retinal rim appeared hyperechoic whereas aqueous, vitreous humor, and nucleus of lens were anechoic. Significant increase (P < 0.05) in all the echobiometric measurement of the ocular distance except central corneal thickness and retinal rim thickness was recorded in the older animals (2 year old) compared to values recorded in animals at one month of age in local nondescript sheep. Significant differences (P < 0.05) in few recorded ocular biometric measurements were also noticed among different sheep breeds. However, the difference in the various ocular biometric measurements between the right and left eyes was nonsignificant. The results of this study provide the base line information regarding the ocular echobiometry in sheep that are relevant to comparative ocular anatomy and can be useful for ultrasonographic evaluation of different ocular affections in sheep.

Use Of Diatomaceous Earth And Copper Oxide Wire Particles To Control Gastrointestinal Nematodes In Lambs, Olivia Jones

Animal Science Undergraduate Honors Theses

Abstract

Anthelmintic resistance (AR) urges alternatives to control gastrointestinal nematodes (GIN). Copper oxide wire particles (COWP) are more efficacious when used with other dewormers and little is known on efficacy of diatomaceous earth (DE) to control gastrointestinal parasites. The objective was to examine the effects of DE and COWP on GIN control. Katahdin lambs (n = 32; ~150 d of age; 25.0 ± 1.8 kg) were randomly assigned to receive: 1) DE fed at an estimated 2% dry matter intake (with the assumption of moderate consumption of bermudagrass forage and provided supplement), 2) 1g COWP, 3) both 2% DE and …

Embryonic Mortality In Sheep: A Review, Pavitra Chundekkad, Barbara Blaszczyk, Tomasz Stankiewicz

Turkish Journal of Veterinary & Animal Sciences

Despite extensive research on the reproductive health of domestic animals, embryonic mortality continues to be a problem that brings about significant losses for both commercial breeding and scientific research. This review paper discusses the genetic and environmental factors that affect embryo mortality rates in sheep along with the different techniques developed to overcome this issue. Additionally, one of the objectives of this study is to emphasize the importance of the use of time-lapse cinematography and color Doppler ultrasonography as quick and reliable methods for early detection of pregnancy and embryonic death.

Prevalence Of Gastrointestinal Parasitic Infections In Cattle And Sheep In Two Regions Ofromania, Catalin Bogdan Sirbu, Kalman Imre, Gheorghe Darabus, Tiana Suici, Bogdan Mates, Sorin Morariu

Turkish Journal of Veterinary & Animal Sciences

Amongst ruminant livestock diseases, the gastrointestinal (GIT) helminths and protozoan parasite infections result in a significant socio-economic concern worldwide. During the period between October 2017 and November 2018, a total of 788 fresh fecal specimens from cattle (n = 303) and sheep (n = 485) were screened for the presence of gastrointestinal helminths and protozoan parasites in two historical regions (Crisana and Banat) of western Romania. Laboratory processing of fecal samples with the simple salt flotation (Willis) and sedimentation techniques, followed by microscopic analysis revealed that 86.1% of cattle and 92.6% of sheep were found to be positive for at …

A Phylogenetic Analysis Of Mycoplasma Strains Circulating In Sheep Pneumonia In Thekars Region Of Turkey, Olcay Öztürkler, Sali̇h Otlu

Turkish Journal of Veterinary & Animal Sciences

This study aimed to investigate Mycoplasma species by cultural and molecular methods in 250 pneumonics and 30 healthy appearance sheep lungs brought from slaughterhouse and butcheries in the Kars region of Turkey. The phylogenetic positioning of the bacterial strains to be obtained in the present study was another remarkable outcome of the study. Mycoplasma isolation was achieved solely in 26 (10.4%) of 250 sheep lungs with pneumonia. Mycoplasma ovipneumoniae was found predominant species in 12 (46.15%) of samples and that was followed by Mycoplasma arginini in 4 (15.38%) of strains. Moreover, two samples were found to be co-infected with the …

Effects Of Activated Charcoal And Zeolite On Serum Lipopolysaccharides And Someinflammatory Biomarkers Levels In Experimentally Induced Subacute Ruminal Acidosis Inlambs, Ali Abbas Nikvand, Mohammad Nouri, Mohammad Razi Jalali, Mohammad Reza Teimory Azadbakht

Turkish Journal of Veterinary & Animal Sciences

Bacterial lipopolysaccharides (LPS) play a key role in the pathogenesis of subacute ruminal acidosis (SARA). Limited attempts have been made to inhibit LPS absorption during SARA. Therefore, this study aimed to evaluate the impacts of oral usage of activated charcoal and zeolite on serum LPS and acute phase proteins (APPs) levels in lambs with SARA. Fifteen lambs were randomly assigned to 3 groups, each containing 5 lambs: control, activated charcoal, and zeolite. In all groups, SARA was induced for 7 days through increasing the crushed corn ration to 52.8% and reducing the rumen pH to the range of 5.7-5.6. In …

The Prevalence Of Gastrointestinal Nematodes In Sheep (Ovis Aries) In The Central Andsouth-Eastern Regions Of Ukraine, Vitalii Melnychuk, Valentyna Yevstafieva, Tetiana Bakhur, Anatoliy Antipov, Diana Feshchenko

Turkish Journal of Veterinary & Animal Sciences

The article investigates into the species composition of agents causing gastrointestinal tract nematodes in sheep. It determines the forms of nematodes communities. For this purpose, 710 sheep were examined, 79.58% of which were infected. The fauna of nematodes was represented by 15 species of helminths from 12 genera: Bunostomum, Oesophagostomum, Nematodirus, Chabertia, Cooperia, Trichostrongylus, Ostertagia, Haemonchus, Trichuris, Aonchotheca, Strongyloides, and Skrjabinema. The indicators of infestation intensity in sheep ranged from 1 to 93 specimens per animal, and the abundance index ranged from 0.01 to 16.96 specimens per animal. The most common nematodes are H. contortus (prevalence, 61.97%), O. circumcincta (59.58%), …

Antimicrobial Resistance And Phenotypic And Molecular Detection Of Extended-Spectrum Ss-Lactamases Among Extraintestinal Escherichia Coli Isolated From Pneumonic And Septicemic Sheep And Goats In Rajasthan, India, Fateh Singh, Ganesh Gangaram Sonawane, Jyoti Kumar, Shivendra Kumar Dixit, Rajendra Kumar Meena, Bhupendra Nath Tripathi

Turkish Journal of Veterinary & Animal Sciences

The present study was planned to isolate Escherichia coli from pneumonic and septicemic sheep and goats and to determine antimicrobial resistance (AMR) patterns of the isolates and evaluate them for the presence of extended-spectrum ß-lactamase (ESBL) using the phenotypic and molecular methods. Pneumonic lung tissues and heart blood samples were collected by performing necropsy on sheep (n = 96) and goats (n = 08). The samples were processed and used for bacterial isolation. E. coli isolates (n = 58) including 53 from 34 sheep and 5 from 3 goats were recovered and identified by the cultural and biochemical characteristics and …

Management Of A Coxiella Burnetii-Infected Sheep Flock After An Outbreak Of Q Fever In Humans, Jana Avbersek, Mateja Pate, Andrej Skibin, Matjaz Ocepek, Brane Krt

Turkish Journal of Veterinary & Animal Sciences

Following an outbreak of Q fever in a group of students who contracted the infection during a training course on a sheep farm, a detailed investigation of the sheep flock involved was conducted. Of 478 flock animals, 60 Coxiella burnetii ELISA-positive and 60 ELISA-negative ewes were selected for the trial and divided into four groups. A month after the initial ELISA screening, all ewes in the flock (except the control group) were vaccinated. Sequentially collected blood samples were tested with ELISA and PCR; feces, milk, manure, bedding, and soil were tested with PCR. The immune response to the vaccination was …

Molecular Detection Of Virulent Mannheimia Haemolytica And Pasteurella Multocida In Lung Tissues Of Pneumonic Sheep From Semiarid Tropics, Rajasthan, India, Fateh Singh, Ganesh Gangaram Sonawane, Rajendra Kumar Meena

Turkish Journal of Veterinary & Animal Sciences

The present study was planned to detect the genetic elements of Mannheimia haemolytica and Pasteurella multocida in pneumonic sheep lungs. Pneumonia was diagnosed on the basis of gross pathological lesions. Lung tissues were collected at necropsy of sheep (n = 96) and subjected to isolation of total DNA. The M. haemolytica-specific PHSSA and Rpt2 genes, and the P. multocidaspecific KMT1 and the Omp87 genes were amplified using polymerase chain reaction (PCR). A housekeeping gene targeting the sheep cellular mitochondrial 12S ribosomal DNA was used as an internal control. PCR reactions were optimized using the positive and negative controls. Gene-specific PCR …

Evaluation Of Biochemical Parameters And Oxidative Stress In Sheep Naturally Infected With Dicrocoelium Dendriticum And Hydatid Cysts, Mi̇yase Çinar, Meral Aydeni̇zöz, Sami̇ Gökpinar, Güzi̇n Çamkerten

Turkish Journal of Veterinary & Animal Sciences

The purpose of the study was to investigate the lipid peroxidation and antioxidant status as well as biochemical profile in sheep naturally infected with Dicrocoelium dendriticum and hydatid cysts. Ten sheep naturally infected with D. dendriticum, 10 sheep naturally infected with hydatid cysts, and 10 healthy sheep were used. The blood and visceral organs of sheep brought to a slaughterhouse in the region of Kırıkkale were checked for D. dendriticum and hydatid cysts. Plasma total protein, albumin, total bilirubin, total cholesterol, Ca, Mg, inorganic P, Cu, Zn, vitamin A, vitamin C, vitamin E, ß-carotene, AST, ALT, GGT, ALP, erythrocyte MDA …

Comparison Of Pre- And Postpartum Serum Leptin, Ghrelin, And Lipid Levels In Sheep, Ethem Mutlu Temi̇zel, Hüseyi̇n Ci̇han, Pinar Levent, Ahmet Saril, Yeşi̇m Özarda, Zeki̇ Yilmaz

Turkish Journal of Veterinary & Animal Sciences

Serum leptin and ghrelin play important roles in energy metabolism and inducing appetite during pregnancy and lactation. We investigated the serum levels of leptin and ghrelin and their relationship with the duration of lactation and serum biochemical responses during the prepartum (last week before lambing) and postpartum periods in sheep. They were also evaluated between pregnant and nonpregnant (control ewes) ewes. For these purposes, pregnant (n = 15) and control Awassi ewes (n = 15) that were 2 years of age were used in this study. Blood samples were collected 1 week before the expected date of lambing (baseline) and …

Comparison Of Different Paternity Test Panels In Sheep, Onur Yilmaz, İbrahi̇m Cemal, Behi̇ç Coşkun, Yusuf Zi̇ya Oğrak, Nezi̇h Ata, Orhan Karaca

Turkish Journal of Veterinary & Animal Sciences

The aims of this study were to evaluate different paternity test panels with 17 microsatellite markers for their effectiveness in paternity analysis and to identify pedigree error rates in the Kangal Akkaraman breed of sheep. The animal material for the study consisted of 175 Kangal Akkaraman sheep, 35 rams, and their 140 offspring, raised on farms and bred using controlled mating. The panels were created according to probability of exclusion (PE) of 17 studied microsatellites. A total of 240 alleles were detected across 17 microsatellite loci. The overall mean value of the polymorphic information content (PIC) (0.78) indicated that the …

Molecular Identification Of Tick-Borne Pathogens In Tickhaemaphysalis Longicornis From Sheep In Henan, China, Yongshuai Peng, Meng Qi, Fuchun Jian, Jinhong Wang, Yali Lv, Jiujian Wei, Rongjun Wang, Longxian Zhang, Changshen Ning

Turkish Journal of Veterinary & Animal Sciences

Tick-borne diseases are one of the most important classes of disease in animal husbandry and cause severe economic losses. In this study, 660 adult female ticks were collected from sheep in nine localities in Henan Province, China. All were identified as Haemaphysalis longicornis, confirmed with light microscopy and PCR amplification. The pathogens identified in these ticks included bacteria of the genus Anaplasma (Anaplasma ovis, A. bovis, A. phagocytophilum) and piroplasmal protozoans (Theileria luwenshuni, Babesia motasi). Our results show the high prevalence of A. bovis (20.4%, 135/660) and T. luwenshuni (14.4%, 95/660) in the ticks from sheep, whereas A. ovis, A. …

Co-Existence Of Bovine Viral Diarrhea And Border Disease Viruses In A Sheep Flock Suffering From Abortus And Diarrhea, Peli̇n Tuncer Göktuna, Gi̇zem Alpay, Eda Baldan Öner, Kadi̇r Yeşi̇lbağ

Turkish Journal of Veterinary & Animal Sciences

Pestivirus infections have a huge economic impact on livestock production. In 2014, an aborted fetus from a sheep flock suffering from abortus and diarrhea was submitted for virological diagnosis. Due to the positive result of the sample for pestivirus and continuing clinical symptoms in the flock, all of the animals were sampled individually. Blood samples for serum and peripheral blood mononuclear cells were collected from 93 animals (5 rams, 26 lambs, and 62 sheep). During sampling 1 ocular and 4 rectal swab samples were obtained from lambs that had a clinical eye problem and diarrhea, respectively. Additionally 5 aborted fetuses …

The Effect Of Combining Vitamin E And C On The Viability Improvement Of Transfected Ovine Spermatogonial Stem Cells After Cryopreservation And Thawing, Hosein Shabani, Mohammad Zandi, Hamideh Ofogi, Mohammad Reza Sanjabi, Khosro Hoseini Pajooh

Turkish Journal of Veterinary & Animal Sciences

The present study aimed to identify whether vitamin E and C and their combination in basal freezing medium improved the viability of postthaw transfected spermatogonial stem cells (SSCs). For this purpose, SSCs were harvested by enzymatic digestion twice. After enrichment and culture on Sertoli cell feeder layer, SSCs were characterized by using alkaline phosphatase staining and expression of oct-4 and c-kit genes as specific stem cell markers. As to the transfection of SSCs, different concentrations of DNA (0.2, 0.4, and 0.8 μg) and turbofect (0.4 and 0.8 μL) in 100 μL of medium were studied. The results showed that cryopreservation …

Comparative Evaluations Of Tear Secretion In Healthy And Infectious Keratoconjunctivitis Romanov Sheep With Schirmer Tear Test And Phenol Red Thread Tear Test, Mustafa Bariş Akgül, Ni̇hat Şindak, Ali̇ Gülaydin, Doğukan Özen, Serpi̇l Kahya Demi̇rbi̇lek, Kadi̇r Sulu

Turkish Journal of Veterinary & Animal Sciences

The aim of this study is to determine the amount of tear secretion in sheep with infectious keratoconjunctivitis and healthy ones in the Siirt region by Schirmer tear test and phenol red thread tear test. A total of 47 Romanov sheep, consisting of sheep with infectious keratoconjunctivitis (n = 6) and healthy sheep (n = 41), constituted the study material. Phenol red thread tear test followed by Schirmer tear test was performed on both eyes of the animals, properly taken at a control visit on their feet. The Schirmer tear test and phenol red thread tear test values were measured …

The Effect Of Management Strategies On Haemonchus Contortus Infections Of Sheep And Goats On Intensively Grazed Pasture, Catherine Werker Pouliot

Honors College

The purpose of this study was to determine how the management strategies used by producers on sheep and goat farms in Maine, New Hampshire, and Vermont could affect the fecal egg counts of the parasite called Haemonchus contortus in their animals. Surveys about management practices and fecal samples from the juvenile and adult populations were collected during the months of June, July, and August in the summer of 2015. Next, the samples were analyzed and fecal egg counts were determined using the McMaster method and a fluorescence-based H. contortus speciation technique. The fecal egg counts and survey answers were compiled …

Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş

Turkish Journal of Veterinary & Animal Sciences

Phospholipase D (PLD) is a major virulence determinant of Corynebacterium pseudotuberculosis. Various studies have focused on the expression of recombinant PLD (rPLD) enzyme in different Escherichia coli strains, but generally a high yield of insoluble protein was reported. The aims of this study were to express soluble rPLD by different methods in E. coli. The rPLD and natural PLD (dPLD) enzymes were concentrated using an ultramembrane cassette system after the efficiencies of these concentrated enzymes were compared. The rPLD enzyme was expressed in One Shot®BL21(DE3) E. coli when induced by IPTG in TY medium. Soluble dPLD and rPLD enzyme hemolytic …

Seroprevalence Of Caseous Lymphadenitis In Kangal Akkaraman Sheep, Öznur Aslan, Kadi̇r Semi̇h Gümüşsoy, İlknur Karaca Bekdi̇k, Aytaç Akçay, Ömer Orkun Demi̇ral

Turkish Journal of Veterinary & Animal Sciences

In this study, the seroprevalence of Corynebacterium pseudotuberculosis, the causative agent of caseous lymphadenitis (CLA) infection, in Kangal Akkaraman sheep flocks was examined. For this purpose, blood samples were collected from 25 different sheep flocks (n = 379). According to the ELISA results, the numbers of CLA-positive and CLA-negative samples were 138 (36.4%) and 241 (63.6%), respectively. CLA positivity was observed in 35.7% of the females (n = 241), 37.7% of the males (n = 138), 40.2% of the sheep with clinical symptoms (n = 184), and 32.8% of the sheep with no clinical symptoms (n = 195) (P > 0.05). …

Cast/Mspi Gene Polymorphism And Its Impact On Growth Traits Of Soviet Merinoand Salsk Sheep Breeds In The South European Part Of Russia, Ivan Fedorovich Gorlov, Nadezhda Vasilevna Shirokova, Alexander Vasilevich Randelin, Valeriya Nikolaevna Voronkova, Natalya Ivanovna Mosolova, Elena Yurevna Zlobina, Yury Anatolevich Kolosov, Nekruz Farkhodovich Bakoev, Mariya Anatolevna Leonova, Sirozhdin Yusufovich Bakoev, Anatoly Yurevich Kolosov, Lubov Vladimirovna Getmantseva

Turkish Journal of Veterinary & Animal Sciences

The purpose of this work was to study MspI polymorphism in the CAST gene in Soviet Merino and Salsk sheep breeds in the South European part of Russia and to find its relation with growth traits. Soviet Merino sheep have been found to have the M and N alleles with a frequency of 0.88 and 0.12 and the MM, MN, and NN genotypes with a frequency of 0.82, 0.12, and 0.06, respectively. Salsk sheep have been established to have the M and N alleles with a frequency of 0.89 and 0.11 and two genotypes, MM and MN, with a frequency …

Comparison Of Pcr Tests For The Detection Of Mycoplasma Agalactiae In Sheep And Goats, Hüban Göçmen, Ruben Sebastian Rosales, Roger David Ayling, Mi̇hri̇ban Ülgen

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to compare PCR tests for the detection of Mycoplasma agalactiae in sheep and goats. Samples obtained from sheep and goats in Turkey were tested to determine the presence of M. agalactiae. Mycoplasma culture and two direct PCR methods based on the uvrC gene and polC gene were compared with PCR of the 16S rRNA gene followed by denaturing gradient gel electrophoresis (PCR/DGGE) on DNA extracted from clinical samples (following culture enrichment). A total of 234 samples were examined: 9.4% and 13.2% were positive for M. agalactiae based on the uvrC gene and polC gene …