Life Sciences Commons^™

Serological Prevalence Of Crimean–Congo Hemorrhagic Fever Virus Infection In Small Ruminants And Cattle In The Gambia, Jerusha Matthews, Arss Secka, David Scott Mcvey, Kimberly A. Dodd, Bonto Faburay

School of Veterinary and Biomedical Sciences: Faculty Publications

Crimean–Congo hemorrhagic fever virus (CCHFV) is a widely distributed tickborne zoonotic agent that infects a variety of host species. There is a lack of information on the true geographic distribution of the prevalence and risk of CCHFV in West Africa. A countrywide cross-sectional study involving 1413 extensively managed indigenous small ruminants and cattle at livestock sales markets and in village herds, respectively, was carried out in The Gambia. In sheep, an overall anti-CCHFV antibody prevalence of 18.9% (95% CI: 15.5–22.8%), goats 9.0% (95% CI: 6.7–11.7%), and cattle 59.9% (95% CI: 54.9–64.7%) was detected. Significant variation (p < 0.05) in the prevalence of anti-CCHFV antibodies at sites in the five administrative regions (sheep: 4.8–25.9%; goats: 1.8–17.1%) and three agroecological zones (sheep: 8.9–32.9%; goats: 4.1–18.0%) was also observed. Comparatively, higher anti-CCHFV antibody prevalence was detected in cattle (33.3–84.0%) compared to small ruminants (1.8–8.1%). This study represents the first countrywide investigation of the seroprevalence of CCHFV in The Gambia, and the results suggest potential circulation and endemicity of the virus in the country. These data provide critical information vital to the development of informed policies for the surveillance, diagnosis, and control of CCFHV infection in The Gambia and the region.

Identifying Barriers To Data Use On U.S. Beef Cow-Calf Operations And Developing Solutions To Improve Cow-Calf Record-Keeping, William Isaac Jumper

Theses and Dissertations

Cattle health and production records (CHPR) are data collected by cattle producers and veterinarians in the form of measurements, observations, counts of events over time, and physiologic attributes that describe individual and group-level health and production. These data are useful to both veterinarians and cattle producers for making evidence-based decisions on cow-calf operations. Currently, there are no uniform, industry-wide methods of capturing and recording CHPR in the U.S. cow-calf industry. Although many cow-calf producers in the U.S. are thought to collect some form of CHPR, it is believed that relatively few are doing so in an electronic manner that facilitates …

Pharmacokinetics Of Single Feeding Of Cannabidiol In Cattle: A Pilot Study, Haley Cornette

Honors College Theses

Cannabidiol (CBD) is a substance that has been used in complementary medicine for many years. However, modern medicine has little knowledge of how this substance is utilized and metabolized in ruminant animals. Regulations on quality assurance and use in animals are lacking, and CBD supplementation in livestock is not approved. If CBD supplements can be shown to be safe and effective, detection in the animal will be important for determining regulation of use. A withdrawal period can then be established to allow time for deterioration of product to safe levels before livestock products enter human markets. This study sought to …

Association Of IsvSa3 With Multidrug Resistance In Salmonella Enterica Isolates From Cattle (Bos Taurus), Gentry L. Lewis, Robert J. Fenton, Etsuko Moriyama, John Dustin Loy, Rodney A. Moxley

School of Veterinary and Biomedical Sciences: Faculty Publications

Salmonella enterica is, globally, an important cause of human illness with beef being a significant attributable source. In the human patient, systemic Salmonella infection requires antibiotic therapy, and when strains are multidrug resistant (MDR), no effective treatment may be available. MDR in bacteria is often associated with the presence of mobile genetic elements (MGE) that mediate horizontal spread of antimicrobial resistance (AMR) genes. In this study, we sought to determine the potential relationship of MDR in bovine Salmonella isolates with MGE. The present study involved 111 bovine Salmonella isolates obtained collectively from specimens derived from healthy cattle or their environments …

Presumptive Hepatogenous Photosensitization In Beef Cattle Winter Grazing A Cover Crop Containing Brassica Spp. Turnips, Matthew M. Hille, Scott A. Fritz, Breanna C. Brown

School of Veterinary and Biomedical Sciences: Faculty Publications

Over a period of ~2 wk, 5 adult beef cows developed severe necrotizing skin lesions on the face and neck shortly after being moved to a field with a cover crop of Brassica spp. turnips. We describe here the clinical signs, hematology, and serum chemistry results, as well as gross and histopathologic findings, from this outbreak. We made a presumptive diagnosis of Brassica-associated liver disease (BALD) based on the similarities in the history and diagnostic findings observed with previously reported cases of BALD from elsewhere in the world. BALD has not been reported previously in cattle in North America, …

A Comparative Study Of Oocyte Between Native Cattle And Buffalo Species In Bangladesh: A Qualitative And Quantitative Approach, Zannatul Naim, Md Saiful Islam, Lam Yea Asad, Md Enayet Kabir

Turkish Journal of Veterinary & Animal Sciences

In vitro embryo production (IVP) can help alleviating reproductive difficulties in cattle and buffalo. This study compared qualitative and quantitative features of native cattle and buffalo ovaries and oocytes to understand them comprehensively. In addition, it was conducted to pick the ideal ovaries for oocyte collection and to assess either maturation affects oocyte diameter or not. The ovaries were collected from the local slaughterhouses of Dhaka city, Bangladesh. Next, the quantitative parameters of ovaries were measured, the follicular fluid was aspired, the oocytes were classified, and the diameters of oocytes were determined in both pre and postmaturation. Here, the results …

A Single-Cell Atlas Of Bovine Skeletal Muscle Reveals Mechanisms Regulating Intramuscular Adipogenesis And Fibrogenesis, Leshan Wang, Peidong Gao, Chaoyag Li, Qianglin Liu, Zeyang Yao, Yuxia Li, Xujia Zhang, Jiangwen Sun, Constantine Simintiras, Matthew Welborn, Kenneth Mcmillin, Stephanie Oprescu, Shihuan Kuang, Xing Fu

Computer Science Faculty Publications

Background: Intramuscular fat (IMF) and intramuscular connective tissue (IMC) are often seen in human myopathies and are central to beef quality. The mechanisms regulating their accumulation remain poorly understood. Here, we explored the possibility of using beef cattle as a novel model for mechanistic studies of intramuscular adipogenesis and fibrogenesis.

Methods: Skeletal muscle single-cell RNAseq was performed on three cattle breeds, including Wagyu (high IMF), Brahman (abundant IMC but scarce IMF), and Wagyu/Brahman cross. Sophisticated bioinformatics analyses, including clustering analysis, gene set enrichment analyses, gene regulatory network construction, RNA velocity, pseudotime analysis, and cell-cell communication analysis, were performed to elucidate …

Effect Of Lipid-Enriched Diet Associated With Bovine Recombinant Somatotropin On Superovulation Response And Embryo Recovery In Heifers, Richelle Matos Oliveira, Anselmo Domingos Ferreira Santos, Francisco Palma Rennó, Camilla Mendonça Silva

Turkish Journal of Veterinary & Animal Sciences

The current study aimed to evaluate the effects of soybean oil supplementation associated with the administration of bovine recombinant somatotropin (rBST) on the population of ovarian follicles, standardization of both the superovulation response and embryo recovery in heifers. We used 40 Holstein heifers submitted to a 2 × 2 factorial, forming the experimental groups: DC-SL (control diet plus saline solution application); OL-SL (diet with soybean oil plus saline application); DC-rBST (control diet plus application of 320 mg rBST) and OL-rBST (diet with soybean oil plus the application of 320 mg rBST). The animals received two applications of 500 mg of …

Assessment Of Diversity Of Antimicrobial Resistance Phenotypes And Genotypes Of Mannheimia Haemolytica Isolates From Bovine Nasopharyngeal Swabs, Hannah F. Carter, Robert W. Wills, Matthew A. Scott, Alexis C. Thompson, Randall S. Singer, John Dustin Loy, Brandi B. Karisch, William B. Epperson, Amelia R. Woolums

School of Veterinary and Biomedical Sciences: Faculty Publications

The threat of bovine respiratory disease (BRD) for cattle operations is exacerbated by increasing prevalence of antimicrobial resistance (AMR) in Mannheimia haemolytica, a leading cause of BRD. Characterization of AMR in M. haemolytica by culture and susceptibility testing is complicated by uncertainty regarding the number of colonies that must be selected to accurately characterize AMR phenotypes (antibiograms) and genotypes in a culture. The study objective was to assess phenotypic and genotypic diversity of M. haemolytica isolates on nasopharyngeal swabs (NPS) from 28 cattle at risk for BRD or with BRD. NPS were swabbed onto five consecutive blood agar plates; …

Spatial Risk Modeling Of Cattle Depredation By Black Vultures In The Midwestern United States, Brandon M. Quinby, Bryan M. Kluever, Grant N. Burcham, Lee Humberg, Landon R. Jones, Marian L. Wahl, Patrick A. Zollner

USDA Wildlife Services: Staff Publications

ock operations through depredation of stock are a cause of human‐wildlife conflict. Management of such conflict requires identifying environmental and non‐environmental factors specific to a wildlife species' biology and ecology that influence the potential for livestock depredation to occur. Identification of such factors can improve understanding of the conditions placing livestock at risk. Black vultures (Coragyps atratus) have expanded their historical range northward into the midwestern United States. Concomitantly, an increase in concern among agricultural producers regarding potential black vulture attacks on livestock has occurred. We estimated area with greater or lesser potential for depredation of domestic cattle by black …

Computed Tomography And Osteological Examination Of The Nuchal Perforations In Byzantine Cattle Skulls, Vedat Onar, Aleksander Chroszcz, Dominik Poradowski, Nazan Gezer İnce, Mehmet Ali̇ Polat, Rahmi̇ Asal

Turkish Journal of Veterinary & Animal Sciences

This study presents computed tomography (CT) and osteological observations of nuchal perforations in the Middle-Late Byzantine cattle skulls, unearthed from Yenikapı Metro and Marmaray excavations (ancient Theodosius harbour), İstanbul, Türkiye. A total of 17 skulls with perforations of various size, ranging from elliptical to triangular, located in the nuchal (posterior) region of protuberantia intercornualis were examined. Rounded edges and smooth inner walls of these perforations indicated that they were formed when the animals were alive (ante mortem). The CT helped to understand the details of the caudal and dorsal part of head as well as the relationships between these perforations …

Genetic Variation At The Olr1, Anxa9, Myf5, Ltf, Igf1, Lgb, Csn3, Pit1, Mbl1,Cacna2d1, And Abcg2 Loci In Turkish Grey Steppe, Anatolian Black, And Eastanatolian Red Cattle, Özden Çobanoğlu, Sena Ardiçli

Turkish Journal of Veterinary & Animal Sciences

Native breeds are excellent sources of genetic variation. Anatolian native breeds are relatives of the first cattle domesticated and are ancestors of many European breeds. Therefore, this study aimed to evaluate the genetic variation of OLR1, ANXA9, MYF5, LTF, IGF1, LGB, CSN3, PIT1, MBL1, CACNA2D1, and ABCG2 markers in Turkish Grey Steppe, Anatolian Black, and East Anatolian Red cattle. The analysis included 367 cattle and the genotyping was performed by the PCR-RFLP. Population genetics indices including heterozygosity, the number of effective alleles, the polymorphism information content, fixation index, and the level of possible variability realization, and moreover, the genetic diversity …

Exploring How Maternal Phosphorus Status Affects Calf Growth And Performance, Elizabeth Lafferty

Animal Science Undergraduate Honors Theses

This study will focus on how maternal phosphorus status of beef heifers affects the growth and performance of their calves. Heifers have been offered free-choice mineral with either 0 or 4% supplemental phosphorus from 30 days after weaning until calving. A study by H. Hilfiker, a University of Arkansas honors student, investigated the effects of these treatments from 30 days after weaning until 60 days after the breeding season when heifers were confirmed to be bred or open. For this developing heifer project 64 crossbred Angus heifers were assigned randomly into 8 groups (8 heifers/ group) before assigning each group …

Genetic Variation In The Bovine Myogenic Determination Factor 1 (G.782g>Apolymorphism) And Its Influence On Carcass Traits In Turkish Grey Steppe Cattle, Sena Ardiçli, Özden Çobanoğlu

Turkish Journal of Veterinary & Animal Sciences

MyoD1 gene is a member of the myogenic differentiation gene family, which plays a crucial role in growth and development. There is a lack of information about the MyoD1variants and their phenotypic influences in Turkish Grey Steppe cattle. Therefore the present study aimed to determine the genetic variability of MyoD1 g.782G>A polymorphism and to evaluate its effects on carcass characteristics in Turkish Grey Steppe cattle. Analyses were conducted on a total of 142 Turkish Grey Steppe bulls. The PCR-RFLP technique was used for genotyping of the g.782G>A polymorphism in exon 1 of the MyoD1 gene. The population genetic …

Novel Heat Index Models For Subtropical Region Based On Daily Milk Production Incrossbred Holstein Cows, Neeraj Kashyap, Atish Kumar Chakravarty, Bharti Deshmukh

Turkish Journal of Veterinary & Animal Sciences

The quantification of the effect of heat stress imposed due to higher rate of heat gain on animal production is a prerequisite while estimating effects of heat stress. Temperature humidity index (THI) models developed empirically using meteorological parameters have been utilized as an indicator of heat stress. This study attempted to develop a heat index model with respect to productivity of the crossbred Holstein cows. More than 1.23 million daily milk yield records of 1860 crossbred cattle spread over a duration of 30 years were utilized. Statistically significant (p < 0.0001) partial regression coefficients were estimated as 0.0748 ± 0.0009 for dry bulb temperature (T$_{db}$) and -0.2228 ± 0.0011 for wet bulb temperature in model 1, -0.0182 ± 0.0005 T$_{db}$ and -0.1205 ± 0.0006 for vapour pressure in model 2, -0.0150 ± 0.0006 for Tdb and -0.1151 ± 0.0006 for dew point temperature in model 3 while as -0.1283 ± 0.0004 for T$_{db}$ and -0.0394 ± 0.0002 for relative humidity in model 4. The ratio of the partial regression coefficients of meteorological parameters (ß2/ß1) was -2.9791 in model 1, 6.8739 in model 2, 7.6731 in model 3 and 0.3073 in model 4. The developed THI models are based on the effect of meteorological parameters on daily productivity of the dairy animals, so the weightages to meteorological parameters were contrastingly different than the reported THI models. These models may successfully be used to evaluate the impact of heat stress on crossbred Holstein cows with respect to milk productivity and many other traits.

2021 Nebraska Beef Cattle Report

Nebraska Beef Cattle Reports

Cow-Calf Nutrition and Management: Metabolic Profile Associated with Pre-Breeding Puberty Status in Range Beef Heifers 5 • Milk Production Impacts on Cow Reproductive and Calf Growth Performance 8 • Genetic Selection Tools: Using Pooling to Capture Commercial Data for Inclusion in Genetic Evaluations 11 • Categorization of Birth Weight Phenotypes for Inclusion in Genetic Evaluations Using a Deep Neural Network 14 • Genetic Parameter Estimates for Age at Slaughter and Days to Finish in a Multibreed Population 16

Growing Calf and Yearling Management: Effects of Monensin and Protein Type on Performance of Yearling Steers Grazing Smooth Bromegrass Pastures 18 • …

In Vitro Gas Production And Fatty Acids Biohydrogenation Of Diets Containing Different Unsaturated Fatty Acids Sources Plus Crude Glycerin, Arturo Samuel Gomez Insuasti, Yury Tatiana Granja-Salcedo, Julian Andres Castillo Vargas, Juliana Duarte Messana, Ana Paula De Oliveira Sader, Telma Teresinha Berchielli

Turkish Journal of Veterinary & Animal Sciences

An in vitro trial was conducted to evaluate the effects of different unsaturated fatty acids (UFA) sources plus crude glycerin (CGL) on gas production and rumen biohydrogenation (RBH). Incubated diet corresponded to diets containing corn silage (30%) and concentrate (70%) composed of corn, urea, mineral salts, CGL, and different UFA sources as follows: no additional fat (NAF), rumen protected fat (RPF), soybean oil, linoleic acid (LA) or alpha-linolenic acid (LN). Methane concentrations in LA and LN were lower compared to NAF (p < 0.001), but when CH4 was expressed as a proportion of total gas, NAF and RPF showed the greatest values among evaluated UFA sources (p = 0.001). 18:1 t11 concentration showed a faster increase from 0 to 1 h and a slower decrease from 1 to 36 h of incubation, irrespective of UFA source. A higher C18:1 t11 production rate was observed from 1 h to 5 h of incubation, where LA and LN have higher values compared to NAF and RPF diets (p < 0.001). Diets with a high content of LA may be efficient as a nutritional approach to reduce methane production and RBH, resulting in positive effects on vaccenic acid concentrations.

The Surveillance Of Gastrointestinal Parasitic Nematodes Of Northwest Arkansas Dairy Cattle Using Traditional And Genetic Parasitological Identification Procedures, Eva M. Wray

Graduate Theses and Dissertations

Traditional and genetic parasitological identification procedures were compared using naturaland artificial nematode parasite infections in Holstein steer calves. The traditional parasitological procedures measured fecal egg counts, coprocultures with subsequent larval collection and adult nematodes collected at necropsy. The genetic identification procedures measured ITS-2 sequences extracted from different stages of nematode development: raw feces, concentrated nematode eggs, third stage larvae and adults. The primary nematodes observed were Cooperia oncophora, Cooperia punctata and Ostertagia ostertagi. The traditional techniques were not significantly different from one another, while the genetic sequencing showed variation amongst the different procedures. The raw feces sequences showed the most …

Associations Among Beef Cattle Genotypes, Neospora Caninum Infection, And Reproductive Performance, Ryan James Page

Graduate Theses and Dissertations

Reproductive performance is crucial for sustained financial success in the beef cattle industry. This dissertation includes a population study that quantified the incidence of Neospora caninum infections in the central region of the United States and tested its relationship with reproductive performance in beef cattle. Trial one of that study concluded that 6.9% of open, replacement heifers (n = 1306) tested seropositive. The second trial in that project found that 9.6% of the breeding age females (n = 500) tested were seropositive for Neospora caninum; and that state in which the cattle lived and age impacted (P < 0.05) infection rate. Breed composition, number of farm dogs on the ranch, and use of total mixed rations were not associated (P > 0.1) with seropositive …

Expression Profiles Of Lincrna And Mrna Related To Milk Yield And Milk Composition Traits In The Milk-Derived Exosomes Of Holstein And Doğu Anadolu Kırmızısı Cows, Selçuk Özdemi̇r, Ömer Eltas, Muhammed Hüdai̇ Çulha

Turkish Journal of Veterinary & Animal Sciences

This study aimed to demonstrate the expression profiles of lincRNAs and mRNAs affecting milk yield and composition traits in the milk-derived exosomes of Holstein and Doğu Anadolu Kırmızısı (DAK) cows. For this purpose, the locations of these specific lincRNAs and mRNAs were confirmed in quantitative trait loci. Then RT-PCR analysis was performed to identify the expression profiles of the lincRNAs and mRNAs. Lastly, correlation analysis was carried out between milk yield data from Holstein and DAK cows and expression levels of the lincRNAs and mRNAs. The findings showed that while lincRNAs and mRNAs associated with milk yield traits were upregulated …

Investigation Of Biotype, Genotype And Virulence Associated Genes Inpasteurella Multocida Capsular Type A Strains From The Respiratory Tract Of Cattle Andtheir Relationship With Disease Cases, Özgül Gülaydin, Kemal Gürtürk

Turkish Journal of Veterinary & Animal Sciences

Pasteurella (P.) multocida, which causes respiratory disease in cattle, can also be found commensally in the upper respiratory tract. Therefore, it is epidemiologically important to determine the possible difference between pathogenic and commensal strains for the diagnosis of the disease. In this study, phenotypic and genotypic characteristics and virulence associated genes of P. multocida capsular type A strains isolated from the respiratory tract of sick and healthy cattle were investigated. Also, the relationship between the disease status of animals and the characteristics of the isolates were evaluated. Total of 51 P. multocida capsular type A strains were biotyped and genotyped …

Prevalence Of Gastrointestinal Parasitic Infections In Cattle And Sheep In Two Regions Ofromania, Catalin Bogdan Sirbu, Kalman Imre, Gheorghe Darabus, Tiana Suici, Bogdan Mates, Sorin Morariu

Turkish Journal of Veterinary & Animal Sciences

Amongst ruminant livestock diseases, the gastrointestinal (GIT) helminths and protozoan parasite infections result in a significant socio-economic concern worldwide. During the period between October 2017 and November 2018, a total of 788 fresh fecal specimens from cattle (n = 303) and sheep (n = 485) were screened for the presence of gastrointestinal helminths and protozoan parasites in two historical regions (Crisana and Banat) of western Romania. Laboratory processing of fecal samples with the simple salt flotation (Willis) and sedimentation techniques, followed by microscopic analysis revealed that 86.1% of cattle and 92.6% of sheep were found to be positive for at …

Evaluation Of Epas1 Variants For Association With Bovine Congestive Heart Failure [Version 1; Peer Review: 2 Approved], Michael P. Heaton, Adam S. Bassett, Katherine J. Whitman, Greta M. Krafsur, Sang M. Lee, Jaden M. Carlson, Halden J. Clark, Helen R. Smith, Madeline C. Pelster, Veronica Basnayake, Dale M. Grotelueschen, B. Vander Ley

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: Bovine congestive heart failure (BCHF) has become increasingly prevalent in feedlot cattle in the Western Great Plains of North America. BCHF is an untreatable complex condition involving pulmonary hypertension that culminates in right ventricular failure and death. A protein variant of hypoxia-inducible factor 2 alpha (HIF2α, encoded by the endothelial PAS domain-containing protein 1 gene, EPAS1) was previously reported to be associated with pulmonary hypertension at altitudes exceeding 2,000 m. Our aim was to evaluate EPAS1 haplotypes for association with BCHF in feedlot cattle raised at moderate altitudes (1,200 m).

Methods: Paired samples of clinical cases and unaffected controls …

Extracellular Vesicles At The Fetal-Maternal Interface In Cattle, Heloisa M. Rutigliano

Funded Research Records

No abstract provided.

Optimized Oral Supplementation Of Vitamins Improves Feed Intake And Rumen Microbial Protein Synthesis In Deoni Cows, Ashwin Kandathil, Srinivas Bandla

Turkish Journal of Veterinary & Animal Sciences

The present study was conducted to optimize oral doses of fat- and water-soluble vitamins in cattle using in vitro gas production model and to ascertain their effects on dry matter intake (DMI), nutrient digestibility, and rumen microbial protein (RMBP) synthesis. Doses optimized using the in vitro model were 3.66, 6.78, 16.3, 41.1, 3.87, 0.323, 4.12, and 0.055 mg/kg DMI, respectively, for thiamine, riboflavin, niacin, pantothenic acid, pyridoxine, biotin, folic acid, and B12 and 500 IU/kg DMI for vitamin K. Thirty-two adult, nonproducing Deoni cows (375 ± 13.4 kg body weight, BW) were randomly divided into four equal groups (n = …

Snake Envenomation In Two Cattle: Clinical/Laboratory Aspects And Treatment Using Equine-Derived Antivenin Of Viperidae, Nuri̇ Altuğ, Cafer Tayer İşler

Turkish Journal of Veterinary & Animal Sciences

In this case report, specific antivenin treatment for Viperidae snake envenomation in two cattle was defined for the first time. The diagnosis of snake envenomation was made according to the history and clinical findings. Clinical examination revealed systemic (tachycardia, dyspnea, cyanosis, hypersalivation, ruminal hypotony) and local (swelling of the leg and head) findings depending on the location of snake bites. Laboratory analysis determined granulocytic leukocytosis, monocytosis, decreased iron binding capacity, and increased CK and LDH enzyme activities. In the specific treatment of cases, equine-derived polyvalent antivenin containing antitoxic immunoglobulins against Viperidae species was administered. Additionally, 5% dextrose, antibiotics, dexamethasone, antihistamines, …

Electrocardiogram And Serum Cardiac Biomarkers Changes In Fmd In Cattle, Ali Abbas Nikvand, Seyedeh Missagh Jalali, Mohammad Nouri, Alireza Ghadrdan Mashhadi, Soroush Hassanpour Amirabadi

Turkish Journal of Veterinary & Animal Sciences

This study was designed to evaluate serum and electrocardiographic (ECG) changes in cattle with foot-and-mouth disease (FMD). Thirty-two cows with clinical signs of FMD and 13 healthy cows were randomly enrolled. After blood sampling, ECG was performed on 23 of the 32 ill cows. Serum circulatory troponin I (cTnI), CPK, LDH, and CK-MB activities were determined in the patient and control groups. Arrhythmias including ventricular tachycardia (VT), sinus tachycardia (ST), atrial fibrillation (AF), and premature ventricular complex (PVC) were seen in 4, 4, 2, and 1 of the patients, respectively. The patients had higher cTnI levels than the control group …

Phenotypic And Genotypic Investigation Of The Heavy Metal Resistance In Escherichia Coli Isolates Recovered From Cattle Stool Samples, Ni̇sa Si̇pahi̇, Emre Karakaya, Serkan İki̇z

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to examine resistance against cadmium (Cd$^{+2}$), copper (Cu$^{+2}$), lead (Pb$^{+2}$), mercury (Hg$^{+2}$), and manganese (Mn$^{+2}$) in Escherichia coli isolates recovered from cattle stool using phenotypic (agar dilution) and genotypic [polymerase chain reaction (PCR)] methods. In addition, the isolates were genotyped via enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR). Escherichia coli was isolated and identified from the 100 stool samples that were examined in the study. It was determined phenotypically that all isolates were sensitive to mercury; 97% of them showed resistance to cadmium; 69% showed resistance to copper; 24% showed resistance to lead; …

Phylogenetic Relationships Of Native Turkish Cattle Breeds Using Microsatellite Markers, Yusuf Özşensoy, Ercan Kurar, Müge Doğan, Zafer Bulut, Mehmet Ni̇zamlioğlu, Ayşe Işik, Aysun Çamlidağ, Vahdetti̇n Altunok

Turkish Journal of Veterinary & Animal Sciences

A total of 20 microsatellite DNA markers were used for genetic characterization and determination of phylogenetic relationships of native cattle breeds of Turkey, including the Anatolian Grey (AG), Anatolian Black (AB), South Anatolian Red (SAR), East Anatolian Red (EAR), Southern Anatolian Yellow (SAY), and Zavot (ZAV). DNA samples were isolated from 271 blood samples using an organic method. Amplified polymerase chain reaction products were separated by capillary electrophoresis and genotypes were determined for 20 microsatellites. A total of 269 different alleles were determined. The lowest (7.80) and highest (10.80) mean allele numbers were observed for the ZAV and SAY populations, …

Serodiagnostic Potential Of Brucella Outer Membrane And Periplasmic Proteins, Aitbay Bulashev, Orken Akibekov, Zhanbolat Suranshiyev, Bakytkali Ingirbay, Saule Eskendirova

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the serological diagnostic potential of the Brucella recombinant outer membrane (rOMP25, rOMP31) and periplasmic proteins (rBP26, rSOD) in a comparative way using an indirect enzyme-linked immunosorbent assay (i-ELISA). Rabbit and/or mouse antibodies to Brucella whole cell and/or soluble protein preparations recognized all recombinant proteins used, which confirms the expression of target antigens in E. coli in active form. The recombinant proteins showed different antigenicity to antibodies of cattle kept on a brucellosis-affected (endemic) farm and/or a new focus of infection. Thus, the presence of anti-Brucella antibodies was confirmed by i-ELISA/rSOD in 79% …