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Diversity Among Rhizobia Effective With Robinia Pseudoacacia L, Janet Mccray-Batzli, William R. Graves, Peter Van Berkum
Diversity Among Rhizobia Effective With Robinia Pseudoacacia L, Janet Mccray-Batzli, William R. Graves, Peter Van Berkum
William R Graves
The diversity of rhizobia that form symbioses with roots of black locust (Robinia pseudoacacia L.), an economically important leguminous tree species, was examined by inoculating seedling root zones with samples of soil collected from the United States, Canada, and China. Bacteria were isolated from nodules, subcultured, and verified to be rhizobia. The 186 isolates varied significantly in their resistance to antibiotics and NaCl, their growth on different carbohydrates, and their effect on the pH of culture media. Most isolates showed intermediate antibiotic resistance, the capacity to use numerous carbohydrates, and a neutral to acid pH response. Isolates had greater similarity …
Stratification Not Required For Tree-Of-Heaven Seed Germination, William R. Graves
Stratification Not Required For Tree-Of-Heaven Seed Germination, William R. Graves
William R Graves
Tree-of-heaven (Ailanthus altissima (Mill.) Swingle) seed germination and shoot dry weight were determined after stratifying samaras at 5 and 25 °C for up to 28 days. Seeds of samaras stratified at 5 °C for 0, 4, and 12 days showed 70, 77, and 96% germination, respectively, 7 days after sowing. Fourteen days after sowing, 90% of nonstratified seeds had germinated, but shoot weight of nonstratified seedlings was 16% less than that of seedlings from fruit stratified at 5 °C for 12 days. Germination 7 days after sowing was greater after stratification at 5 °C than at 25 °C, but the …