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Full-Text Articles in Life Sciences
Migration Frustrations Of Mir-146a Regulation, Emma Baccus, Victoria Gahman, Hannah Phillips, Shannon Rappaport, Alyssa Reiter, Kaleb M. Pauley
Migration Frustrations Of Mir-146a Regulation, Emma Baccus, Victoria Gahman, Hannah Phillips, Shannon Rappaport, Alyssa Reiter, Kaleb M. Pauley
The Research and Scholarship Symposium (2013-2019)
The autoimmune disease, Sjögren’s Syndrome (SS), causes the degradation of salivary and lacrimal glands due to an influx of immune cells. In previous studies, a significant increase in miR-146a was observed in the peripheral blood mononuclear cells of SS patients. Since immune cell infiltration is critical in SS pathogenesis, the following research examines the effect of miR-146a on cell migration. We hypothesize that transfecting THP-1 human monocytes with synthetic miR-146a will downregulate migration of the monocytes based on other studies stating that miR-146a downregulates migration in vivo. In order to execute our experiment, we transfected THP-1 cells with synthetic miR-146a …
The Regulatory Effect Of Semaphorin 7a On Proliferation And Migration In Human Umbilical Vein Endothelial Cells, Steven R. Fleming
The Regulatory Effect Of Semaphorin 7a On Proliferation And Migration In Human Umbilical Vein Endothelial Cells, Steven R. Fleming
Seaver College Research And Scholarly Achievement Symposium
Semaphorin 7A (SEMA 7A), a factor originally identified as regulating axon growth, has recently been implicated as a pro-angiogenic factor. The molecular mechanisms for this ability to stimulate angiogenesis have not been identified. This study examines if SEMA 7A can have a direct effect on vascular endothelial cells or whether it indirectly induces angiogenesis through stimulation and recruitment of macrophages as has been suggested. Using human umbilical vein endothelial cells (HUVECs), the ability of SEMA 7A to affect proliferation and migration was examined. HUVECs were exposed to SEMA 7A directly or to conditioned media collected from macrophages exposed to SEMA …