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Atpase Regulation In The Maltose Transporter, Alister D. Gould
Atpase Regulation In The Maltose Transporter, Alister D. Gould
Electronic Thesis and Dissertation Repository
This thesis investigates the mechanism of activity-coupling in the maltose transporter of Escherichia coli (MalFGK2); the way ATP hydrolysis is prevented in the absence of maltose, and then enabled to drive maltose transport. Like other ATP binding cassette importers, MalFGK2 requires substrate to be presented by a peripheral substrate-binding protein, in this case the maltose binding protein (MBP). MBP predominantly adopts an ‘open’ resting state, but undergoes a rotation of its two domains to a ‘closed’ state after maltose binding. In the closed state MBP is able to activate MalFGK2 to stimulate ATP hydrolysis and maltose …