Biochemical and Biomolecular Engineering Commons^™

Addition Of Fermentation Experiment To Unit Operations Laboratory, Jeremy T. Rone

Electronic Theses and Dissertations

With the growth of the distillery and brewery industries and the potential jobs created for chemical engineers, it is imperative to equip the chemical engineering graduates from the University of Louisville with knowledge of bioreaction kinetics and experience in performing calculations to solve for concentration profiles and reaction rates. To accomplish this, it is recommended to add a fermentation experiment to the Unit Operations Laboratory II course.

Four groups of students from the Spring 2020 Unit Operations Laboratory II course performed the fermentation experiment. They were able to successfully complete all lab requirements as well as analyze the results and …

Materials For Diabetes Therapeutics, Kaitlin M. Bratlie, Roger L. York, Michael A. Invernale, Robert Langer, Daniel G. Anderson

Kaitlin M. Bratlie

This review is focused on the materials and methods used to fabricate closedloop systems for type 1 diabetes therapy. Herein, we give a brief overview of current methods used for patient care and discuss two types of possible treatments and the materials used for these therapies-(i) artificial pancreases, comprised of insulin producing cells embedded in a polymeric biomaterial, and (ii) totally synthetic pancreases formulated by integrating continuous glucose monitors with controlled insulin release through degradable polymers and glucose-responsive polymer systems. Both the artificial and the completely synthetic pancreas have two major design requirements: the device must be both biocompatible and …

Glucose Biosensor Using Electrospun Mn2o3-Ag Nanofibers, Shan Huang

Master's Theses

The highly porous Mn₂O₃-Ag nanofibers were fabricated by a facile two-step procedure (electrospinning and calcination) and then employed as the immobilization matrix for glucose oxidase (GOD) to construct an amperometric glucose biosensor. A notable enhancement of direct electron transfer between GOD and the electrode is observed at the Mn₂O₃-Ag-GOD modified electrode with a fast electron transfer rate constant. The biosensor also shows fast response to glucose, high sensitivity (40.60 μA×mM^-1×cm^-2), low detection limit (1.73 µM at S/N=3), low K_m,app value and excellent selectivity. These results indicate that …

Effects Of Carbon Nanotube-Tethered Nanosphere Density On Amperometric Biosensing: Simulation And Experiment, Jonathan C. Claussen, James B. Hengenius, Monique M. Wickner, Timothy S. Fisher, David M. Umulis, D. Marshall Porterfield

Jonathan C. Claussen

Nascent nanofabrication approaches are being applied to reduce electrode feature dimensions from the microscale to the nanoscale, creating biosensors that are capable of working more efficiently at the biomolecular level. The development of nanoscale biosensors has been driven largely by experimental empiricism to date. Consequently, the precise positioning of nanoscale electrode elements is typically neglected, and its impact on biosensor performance is subsequently overlooked. Herein, we present a bottom-up nanoelectrode array fabrication approach that utilizes low-density and horizontally oriented single-walled carbon nanotubes (SWCNTs) as a template for the growth and precise positioning of Pt nanospheres. We further develop a computational …

Rational Pathway Engineering Of Type I Fatty Acid Synthase Allows The Biosynthesis Of Triacetic Acid Lactone From D-Glucose In Vivo, Wenjuan Zha, Zengyi Shao, John W. Frost, Huimin Zhao

Zengyi Shao

Metabolic pathway engineering is a powerful tool to synthesize structurally diverse and complex chemicals via genetic manipulation of multistep catalytic systems involved in cell metabolism. Here, we report the rational design of a fatty acid biosynthetic pathway, Brevibacterium ammoniagenes fatty acid synthase B (FAS-B), that allows the microbial synthesis of triacetic acid lactone (TAL) from an inexpensive feedstock, D-glucose. TAL can be chemically converted to phloroglucinol, which is a core structure for the synthesis of various high value bioactive compounds and energetic compounds such as 1,3,5-triamino-2,4,6-trinitrobenzene (TATB). Synthesis of phloroglucinol from d-glucose using this combined biological and chemical synthesis may …