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Full-Text Articles in Biochemical and Biomolecular Engineering
Real-Time Monitoring Of Cell Death Progress Using Capacitance Spectroscopy, Suyang Wu
Real-Time Monitoring Of Cell Death Progress Using Capacitance Spectroscopy, Suyang Wu
Electronic Theses and Dissertations
Biologics, including the monoclonal antibody (mAb), has experienced rapid development in the last decade. However, the price of biologics is often prohibitively high because of the low process efficiency. Delaying the inevitable cell death improves the productivity of upstream bioprocessing, whose success relies on monitoring the cell death onset that indicates the timing for preventive actions.
This study proposes to develop a real-time monitoring model that quantifies the dying cell percentage in lab-scale bioreactors using capacitance spectroscopy. The capacitance spectroscopy contains cell death-related information due to various physical properties changes during the cell death process, e.g., cytoplasmic conductivity change. The …
Engineering Modularity Of Ester Biosynthesis Across Biological Scales, Hyeongmin Seo
Engineering Modularity Of Ester Biosynthesis Across Biological Scales, Hyeongmin Seo
Doctoral Dissertations
Metabolic engineering and synthetic biology enable controlled manipulation of whole-cell biocatalysts to produce valuable chemicals from renewable feedstocks in a rapid and efficient manner, helping reduce our reliance on the conventional petroleum-based chemical synthesis. However, strain engineering process is costly and time-consuming that developing economically competitive bioprocess at industrial scale is still challenging. To accelerate the strain engineering process, modular cell engineering has been proposed as an innovative approach that harnesses modularity of metabolism for designing microbial cell factories. It is important to understand biological modularity and to develop design principles for effective implementation of modular cell engineering. In this …