Engineering Commons^™

Acceleration Of The Maturation Of Tissue Engineered Grafts By Dynamic Force Stimulation Or Gene Therapy, Shila Taylor

All Theses

Over 16 million people suffer from osteoarthritis (OA). As the body ages OA and other cartilage diseases create an imbalance of anabolic and catabolic cell activities in cartilage and or over time the cartilage is damaged and wears away which creates lesion that resulting in pain. Most clinical surgeries are either temporary or have not gone through trials to test their success. For a long term solution researchers have been looking at replacing cartilage with a tissue engineered graft. One of the major problems to date has been is the strength of the scaffold.
Natural cartilage needs loading for its …

Optical Force Based Cancer Cell Identification, Justin Roman

All Theses

We present the basis of a novel, non-invasive technique for cell diagnostics which utilizes the optical force generated by a weakly focused laser beam to distinguish cells based on their size, structure, composition, and membrane properties. Cell populations of different types, biological states or with different treatments can be studied. This research focuses on two particular instances where other methods of cell sorting, such as those that require fluorescent markers, are not ideal. What's more, this research emphasizes the ability to sort morphologically similar cells that are identical to the naked eye, but phenotypically different on the molecular level. The …

Development Of An In Vitro Test System For Breast Cancer Research, Chih-Chao Yang

All Dissertations

In the United States, breast cancer is the third most common cause of cancer death (after lung cancer and colon cancer). In 2007, breast cancer is expected to cause 40,910 deaths (7% of cancer deaths; almost 2% of all deaths) in the U.S. The long term goal of this project is to develop an in vitro test system that can be used to develop breast cancer vaccine or screen breast cancer chemotherapy. This dissertation was driven by four objectives and it can be thought as a toolbox that provides practical experimental design and lab work for the development of an …

Adipocyte Response To Injectable Beads Engineered For Breast Tissue Reconstruction, Katherine Neser

All Theses

It is estimated that over 150,000 patients will be diagnosed with invasive breast cancer in 2007. Patients must undergo a mastectomy or a lumpectomy to remove the cancerous tissue, but only a mastectomy allows breast reconstructive surgery. Due to the limitations of current reconstruction options, new alternatives are being explored. Injectable materials have been suggested for breast tissue reconstruction because of their versatility. Cells cultured on injectable beads form cell carriers that may be mixed with a hydrogel, resulting in a construct that may be injected through a syringe to restore normal tissue mass. This solution offers breast cancer patients …

Mems Capacitive Strain Sensing Elements For Integrated Total Knee Arthroplasty Prosthesis Monitoring, Boyd Mccutchen Evans Iii

Doctoral Dissertations

Measuring the in vivo load state of Total Knee Arthroplasty (TKA) components is required to understand the structural environment and wear characteristics of the devices. The ability to acquire this information gives tremendous insight into the mechanics of the joint replacement prosthesis. Data corresponding to normal loads, in-plane loads, shear loads, load center, contact area, and the rate of loading is needed to fully understand the kinematics and kinetics of the orthopedic implant. In this research, a novel sensing system has been developed which is capable of fully characterizing three-dimensional strain and stress at a single location.

Capacitance-based sensors were …

Theoretical Modeling And Experimental Validation Of In Vivo Mechanics For Subjects Having Variable Cervical Spine Conditions, Fei Liu

Doctoral Dissertations

The objective of this study was to use the state-of-art 3D-to-2D registration technologies including fluoroscopic, CT and MRI methods to analyze 2D and 3D in vivo kinematics of the whole cervical spine under variable conditions; and use inverse dynamic model based on Kane’s dynamics to predict their 2D and 3D in vivo interactive contact and muscular forces. Totally, forty patients (ten having normal cervical spines, ten having degenerative cervical spines, ten having anterior cervical decompression and fusion (ACDF), and ten having cervical artificial disc replacement (CADR)) were enrolled into 2D study and three patients (one having normal cervical spines, one …

Characterization Of A Biomimetic Calcium-Deficient Hydroxyapatite-Bacterial Cellulose Composite, Stacy Hutchens

Doctoral Dissertations

Bone is the second most implanted tissue next to blood causing approximately 2.2 million people to receive bone grafts each year. Developing safe synthetic bone grafts allows quick and safe restoration of bone function while avoiding the surgical risks associated with bone autografting (self donation), and risks of disease transmission and immunogenic response associated with allografts (bone donated from other humans) and xenografts (grafts derived from animal tissue). This dissertation entails the study and development of a novel potential synthetic bone graft consisting of a composite of calcium-deficient hydroxyapatite (CdHAP) biomimetically deposited in a bacterial cellulose (BC) hydrogel.

To determine …

Applied Fourier Transform Near-Infrared Techniques For Biomass Compositional Analysis, Lu Liu

Masters Theses

A new method for rapid chemical analysis of lignocellulosic biomass was developed using Fourier transform near-infrared (FT-NIR) spectroscopic techniques. The new method is less time-consuming and expensive than traditional wet chemistry. A mathematical model correlated FT-NIR spectra with concentrations determined by wet chemistry. Chemical compositions of corn stover and switchgrass were evaluated in terms of glucose, xylose, galactose, arabinose, mannose, lignin, and ash. Model development evaluated multivariate regressions, spectral transform algorithms, and spectral pretreatments and selected partial least squares regression, log(1/R), and extended multiplicative signal correction, respectively. Chemical composition results indicated greater variability in corn stover than switchgrass, especially among …

Image And Sieve Analysis Of Biomass Particle Sizes And Separation After Size Reduction, Yuechuan Yang

Masters Theses

Improved physical separation of biomass concentrates higher-value components, returns unused plant components to the soil, and provides a more efficient platform for downstream industrial users. Sieving and Image Analysis (IA) were studied to evaluate biomass particle sizes, particle size distribution, and potential separation of biomass materials. Switchgrass, wheat straw, and corn stover were used in the study. Particles prepared by a knife mill equipped with various screen sizes were subject to sieving test and IA. Results showed that all three biomass materials would be analyzed using the fast, easy and accurate IA. Biomass particle physical properties, such as length, width, …

Glycosaminoglycan Stabilization In Bovine Pericardium, Lauren Browne

All Theses

Glutaraldehyde crosslinked bovine pericardium has been used for fabrication of bioprosthetic heart valves as well as cardiac patches for soft tissue repair. However, calcification and limited mechanical stability result in shortened life for the prostheses. Previous research has shown that glutaraldehyde crosslinking does not stabilize glycosaminoglycans (GAGs) and that GAGs are lost from porcine bioprosthetic heart valves[1]. Bovine pericardial tissue is composed of an amorphous network of collagen and elastin fibers, proteoglycans, and GAGs. The GAGs of bovine pericardium include dermatan sulfate, chondroitin sulfate, and hyaluronan [2].
It is hypothesized that Glut does not stabilize GAGs in pericardium and loss …

Development Of The Telemetrical Intraoperative Soft Tissue Tension Monitoring System In Total Knee Replacement With Mems And Asic Technologies, Gary To

Masters Theses

The alignment of the femoral and tibial components of the Total Knee Arthoplasty (TKA) is one of the most important factors to implant survivorship. Hence, numerous ligament balancing techniques and devices have been developed in order to accurately balance the knee intra-operatively. Spacer block, tensioner and tram adapter are instruments that allow surgeons to qualitatively balance the flexion and extension gaps during TKA. However, even with these instruments, the surgical procedure still relies on the skill and experience of the surgeon. The objective of this thesis is to develop a computerized surgical instrument that can acquire intra-operative data telemetrically for …

Gene Synthesis, Cloning, Expression, Purification And Biophysical Characterization Of The C2 Domain Of Human Tensin, Kiran Sukumar Gajula

Doctoral Dissertations

Tensin is a large "docking" protein found in the adhesive junctions of animal cells and recruited early in the development of cell-substrate contacts. There it binds to the cytoplasmic domain of integrin β1 and caps the barbed ends of filamentous actin. This forms a rational basis for its implication in a direct role in the mechanics of membrane-cytoskeleton interactions. Tensin provides a physical link between the actin cytoskeleton, integrins, and other proteins at the cell-substrate contacts. Its overall biochemical properties are a function of its domain composition and architecture, i.e., the domains that are present and their relative positions in …

Rediseño, Construcción Y Optimización Del Sistema De Control Para Pie De Prótesis Transfemoral Semiactiva, Yesid Rozo Pinzon

Ingeniería en Automatización

No abstract provided.

Novel Model Of Cerebrospinal Fluid Dynamics Based On Hemodynamically Driven Cyclic Brain Compliance Variation, Bruno A. Mantilla

Dissertations

This study provides a novel explanation for the CerebrQ-Spinal Fluid (CSF) flow pattern observed in phase contrast cine-MRJ studies. CSF dynamics has been traditionally explained as a bulk flow from the site of production to the site of absorption. Studies done with phase contrast cine-MRI show a more complex CSF movement, that is not explainable by the bulk flow paradigm. This study describes a mechanism explaining how the energy delivered by the heart in each cycle is responsible not only for the blood flow, but also for the CSF circulation. This mechanism is based on a cyclic variation of brain …

Theoretical Framework For Predicting Joint Reaction And Ground Reaction Forces In A Dynamic Pendulum Tree Model Of Human Motion, Peyman Rajai

Theses

Lagrangian dynamics and the method of superfluous coordinates are applied to find ground and joint reaction forces on the human body modeled as a general branched 2-D pendulum tree system with arbitrary segments and arbitrarily distributed point masses. A theoretical framework is established for predicting these constraint forces during human motion and consequently their effects on dynamics, dynamic stability, energy efficiency and the potential of these forces to produce joint injury and/or pain. Applications to human walking are initiated. During idealized phases where there is only single point contact of the stance leg with the ground such as just after …

Glycosaminoglycan Stabilization Reduces Tissue Buckling In Bioprosthetic Heart Valves, Sagar Shah

All Theses

Currently, bioprosthetic heart valves are crosslinked with glutaraldehyde to prevent tissue degradation and to reduce tissue antigenicity. Glutaraldehyde forms stable crosslinks with collagen via a Schiff base reaction of the aldehyde with an amine group of the hydroxylysine/lysine in collagen. However, within a decade of implantation, 20-30% of these bioprostheses will become dysfunctional and over 50% will fail due to degeneration within 12-15 years post-operatively.
Gylcosaminoglycans, a major constituent of valvular tissue, play an important role in maintaining a hydrated environment necessary for absorbing compressive loads, modulating shear stresses, and resisting tissue buckling. One of the disadvantages of glutaraldehyde crosslinking …

An In Vitro Evaluation Of Dbm As A Tissue Engineered Scaffold, Stephanie Arnold

All Theses

Over 500,000 bone graft procedures are performed each year in the United States. Bone grafting involves a surgical procedure to replace missing bone. Problems can arise with donor and defect sites during and after surgery, sometimes resulting in poor clinical results. The development and optimization of bone graft substitutes via a tissue engineering approach could markedly improve bone graft surgical outcome. Demineralized bone matrix (DBM), a bone graft material, is currently used in a clinical setting but has variable success rates.
The primary objective of the research presented in this thesis was to assess the cellular activity of D1 mouse …

Differentiation Modulation Of Adult Stem Cells In An Adipose System, Aditya Chaubey

All Dissertations

The need for soft tissue reconstruction or augmentation has increased continuously over the years. This need is compounded by patients suffering from post-traumatic repair and congenital soft-tissue deformities. All the current options available to treat the soft tissue deformities have inherent difficulties associated with them. Hence, more research is needed to come up with a better solution to this problem which is only going to increase in magnitude. Tissue engineering is a relatively new technique which has the potential to deliver a cell-based device which can overcome the problems associated with traditional therapies. However, before it becomes clinically viable we …

Activation Of Systemic Adhesion Molecules And C Reactive Protein In Response To Biomaterial Particle Induced Cytokine Production, Robin Graham

All Dissertations

Chronic inflammation has been identified as a major contributor to many diseases including, atherosclerosis, osteoporosis, dementia, Alzheimer's disease and type 2 diabetes, to name a few. Many of the same markers of inflammation found in the diseases listed above, including IL-1, IL-6, TNF-α, NF-B and adhesion molecules, have also been found in wear debris induced osteolysis. While the local response to biomaterial wear debris has been characterized quite extensively, very little is known about the systemic effect of biomaterial wear debris on the inflammatory system. The objectives of this study were to evaluate the in vitro and in vivo inflammatory …

In Situ Photopolymerized Hydrogels For Enhancing Protein Delivery, Chien-Chi Lin

All Dissertations

In recent years, there has been immense interest in the utilization of photopolymerized hydrogels as carriers for controlled protein delivery and cell scaffolds for tissue engineering applications. Although poly(ethylene glycol) (PEG)-based hydrogels formed from mild photopolymerization methods have been suggested as biocompatible matrices that allow for safely encapsulating biomolecules including proteins, peptides, DNA, and cells, the adverse effects of photopolymerization reactions on the encapsulated proteins have largely been overlooked. In addition, conventional hydrophilic hydrogels fail to effectively control protein delivery rates due to their high permeability. These two problems are critical since the delivery of protein therapeutics from hydrogel matrices …

Non-Boltzamann Sampling For The Accurate Calculation Of Peptide-Surface Adsorption Free Energy, Feng Wang

All Dissertations

%Abstract
The objectives of this project are 1) the setup and refinement of peptide-SAM surface model systems with explicit water using CHARMM and 2) the development of up-to-date simulation protocols for the accurate calculation of adsorption free energy by incorporating the recent development of non-Boltzmann sampling methods in molecular dynamics and applying it for the calculation of adsorption free energy of short peptides onto well-characterized self-assembled monolayers, which is important for understanding protein/surface interactions.
A software package called the Simulation Template Engine for Peptides at Surfaces (STEPS) was developed for the fulfillment of the first objective. It facilitates the automatic …

Design And Evaluation Of A Non-Intrusive Corn Population Sensor, Haizhou Li

Doctoral Dissertations

Specific objectives of this study were to develop, prototype, and test a corn population sensor. Both intrusive mechanical and non-intrusive capacitive techniques have been used to develop the stalk population sensors in previous research. However, neither could generate consistent performance. The mechanical method required high maintenance and resulted in significant underestimations of stalk counts. The performance of capacitive systems was limited by inadequate sensing distance, especially at low stalk moisture levels.

In this research, the sensitivity of the capacitive sensor was optimized for corn stalks. This system utilized a single-sided capacitive sensor, Wien bridge oscillator, phase-locked loop, and an operational …

Improving Pediatric Cardiology Consultation Methods By Introducing Digital Interactive 3-D Heart Models: A Proof Of Concept Study, Adam Verigan

USF Tampa Graduate Theses and Dissertations

The purpose of a pediatric cardiology consultation is to inform, or educate, the patient and family of all aspects surrounding a certain congenital heart defect. Consultation education methods and materials may include verbal descriptions, two-dimensional (2-D) heart diagrams, and take-home pamphlets. Because the human heart is a complex three-dimensional (3-D) object, the problem lies within the clarity to which these methods are performed by the doctors and understood by the patients and families. Therefore, during a consultation the cardiologist must a) possess the ability to describe a defect visually as well as verbally and b) ensure that the patient and …

Development And Analysis Of An Internet Browsing Utilizing The Overscan Technique For Persons With Physical Disabilities, Patrick Paul Clerkin

Doctoral Dissertations

Persons with a severe disability often use scanning as an indirect selection technique for operating augmentative and alternative communication aids and computer access. For information that can be organized in advance, including lists of communication elements such as words and phrases, users often employ rate enhancing scanning methods like the row-column scanning technique. However, row-column scanning requires selection elements to be grouped into defined rows and columns, and therefore does not work well with Internet browsing due to the non-grouped layout of HTML pages.

This work attempts to develop an improved scanning technique for Internet browsing by designing interfaces to …

Computer Simulation And Human Experiment On The Laplacian Electrocardiogram (Ecg), Ting Chen

Doctoral Dissertations

The electrocardiogram (ECG) provides useful global temporal assessment of cardiac activity, but has limited spatial capabilities. Laplacian electrocardiogram (LECG) and body surface Laplacian mapping (BSLM), improvements over ECG provides high spatiotemporal distributed information about cardiac electrical activation.

This project was divided into two parts: computer simulation and human experiment. In the computer simulation, a comparison of the performance of the tripolar and bipolar as well as spline LECG and BSLMs for localizing and imaging the cardiac electrical activation has been investigated. A simple planar surface model and a simplified eccentric heart-torso sphere-cylinder homogeneous volume conductor model were developed. Multiple dipoles …

Central And Peripheral Autonomic Influences : Analysis Of Cardio-Pulmonary Dynamics Using Novel Wavelet Statistical Methods, Anne Marie Petrock

Dissertations

The development and implementation of novel signal processing techniques, particularly with regard to applications in the clinical environment, is critical to bringing computer-aided diagnoses of disease to reality. One of the most confounding factors in the field of cardiac autonomic response (CAR) research is the influence of the coupling of respiratory oscillations with cardiac oscillations.

This research had three objectives. The first was the assessment of central autonomic influence over heart rate oscillations when the pulmonary system is damaged. The second was to assess the link between peripheral and central autonomic control schema by evaluating the heart rate variability (HRV) …

In Vitro Feasibility Testing Of Floating Light-Activated Minroelectrical Stimulators, Ammar Riad Abdo

Theses

One of the major challenges of neural stimulation is the mechanical stress and resulting trauma induced on the implanted electrodes by the constant movement of the interconnects. A potential way of eliminating interconnects is to use floating micro-stimulators that can be activated through optical means. As a method of energy transfer to the micro-stimulator, we propose to use a laser beam at near infrared (NIR) wavelengths.

There are two main objectives in this project to test the feasibility of the main approach; investigate the charge injection capacity of titanium nitride (TiN) and iridium oxide (IrOx) as potential contact materials, and …

Automatic Registration And Segmentation Of Abdominal Images And Detection Of Pancreatic Cancer, Girish Kumar Maniprasad

Theses

Localized and detailed analyses of 3D abdominal images obtained through different imaging modalities help greatly in determining the progression of a disease or for postoperative treatment / evaluation. However, such analyses become difficult and sometimes unfeasible due to the effects of patient motion and breathing. This is particularly evident during analysis of the pancreas for cancer, due to its proximity to other intra-abdominal organs. Within subject registration thus becomes imperative for pathological analysis of pancreatic cancer. An intensity-based, global image registration algorithm was developed in the present work, for registration of pancreatic abdominal images. The registration algorithm was automatic and …

Adaptable Videogame Platform For Interactive Upper Extremity Rehabilitation, Sally M. Jensen

Theses

The primary objective of this work is to design a recreational rehabilitation videogame platform for customizing motivating games that interactively encourage purposeful upper extremity gross motor movements. Virtual reality (VR) technology is a popular application for rehabilitation therapies but there is a constant need for more accessible and affordable systems. We have developed a recreational VR game platform can be used as an independent therapy supplement without laboratory equipment and is inexpensive, motivating, and adaptable. The behaviors and interactive features can be easily modified and customized based on players' limitations or progress.

A real-time method of capturing hand movements using …

Vibrotactile Sensory Substitution For Post-Stroke Rehabilitation, Carlos X. Rosado

Theses

The aftermath of a stroke leaves people with side effects such as speech and hearing problems, and loss of sensation in one side of their body. Sensory feedback in the hand is used to assess if the individual is using appropriate grip force to hold and use objects; lack of sensory feedback can lead to dropped or damaged objects, and possible hand injury. Existing force biofeedback devices are overly complex and difficult to use in the home. The goal of this project is to provide increased environmental awareness of hand grip force for individuals with reduced hand sensation. Although hand …