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Bacterial Diversity Within The Human Subgingival Crevice, Ian Kroes, Paul W. Lepp, David A. Relman

U.S. Department of Veterans Affairs Staff Publications

Molecular, sequence-based environmental surveys of microorganisms have revealed a large degree of previously uncharacterized diversity. However, nearly all studies of the human endogenous bacterial flora have relied on cultivation and biochemical characterization of the resident organisms. We used molecular methods to characterize the breadth of bacterial diversity within the human subgingival crevice by comparing 264 small subunit rDNA sequences from 21 clone libraries created with products amplified directly from subgingival plaque, with sequences obtained from bacteria that were cultivated from the same specimen, as well as with sequences available in public databases. The majority (52.5%) of the directly amplified 16S …

Chemical Toxins (Field Manual Of Wildlife Diseases), Milton Friend

Other Publications in Zoonotics and Wildlife Disease

Many kinds of potentially harmful chemicals are found in environments used by wildlife. Some chemicals, such as pesticides and polychlorinated biphenyls (PCBs), are synthetic compounds that may become environmental contaminants through their use and application. Other materials, such as selenium and salt, are natural components of some environments, but contaminants of others. Natural and synthetic materials may cause direct poisoning and death, but they also may have adverse effects on wildlife that impair certain biological systems, such as the reproductive and immune systems. This section provides information about some of the environmental contaminants and natural chemicals that commonly cause avian …

Genetic And Structural Analyses Of Cytoplasmic Filaments Of Wild-Type Treponema Phagedenis And A Flagellar Filament-Deficient Mutant, Jacques Izard, William A. Samsonoff, Mary Beth Kinoshita, Ronald J. Limberger

Department of Food Science and Technology: Faculty Publications

Unique cytoplasmic filaments are found in the treponeme genus of spirochete bacteria. Their function is unknown, but their location underneath the periplasmic flagellar filaments (PFF) suggests a role in motility and/or cell structure. To better understand these unique structures, the gene coding for the cytoplasmic filaments, cfpA, was identified in various treponemal species. Treponema phagedenis cfpA was 2,037 nucleotides long, and the encoded polypeptide showed 78 to 100% amino acid sequence identity with the partial sequence of CfpA from T. denticola, T. vincentii, and T. pallidum subsp. pertenue. Wild-type T. phagedenis and a PFF-deficient isolate were analyzed by electron microscopy …

Deliberate Introductions Of Species: Research Needs, John J. Ewel, Dennis J. O'Dowd, Joy Bergelson, Curtis C. Daehler, Carla M. D'Antonio, Luis Diego Gómez, Doria R. Gordon, Richard J. Hobbs, Alan Holt, Keith R. Hopper, Colin E. Hughes, Marcy Lahart, Roger R. B. Leakey, William G. Lee, Lloyd L. Loope, David H. Lorence, Svata M. Louda, Ariel E. Lugo, Peter B. Mcevoy, David M. Richardson, Peter M. Vitousek

Svata M. Louda Publications

Research questions about introductions

Several research questions need to be answered to help ensure that proposed introductions are done wisely and safely.

Guarding against risks without sacrificing benefits:
How can the potential benefits and costs of introductions best be evaluated in economic, environmental, and social terms?
Should all introductions be regulated?
How different must organisms or recipient ecosystems be from those assessed previously to warrant independent assessment?
When is it appropriate to assess and regulate taxa other than species?
What are appropriate ecological and political boundaries for regulation?

Alternatives to introductions:
How and when can indigenous organisms be domesticated so …

The Probe, Issue 202 – July 1999

The Probe: Newsletter of the National Animal Damage Control Association

Bird Strike USA Meeting a Success -- Eugene LeBoeuf, Bird Strike Scientist, HQ Air Force Safety Center, KirtlandAFB, New Mexico, and Past President, NADCA
Position Available: Wildlife Biologist, JFK Airport
Hawk Starts Grass Fire
Juicy Fruit Gum Remedy Story Persists
Cambodian Artifacts Threatened by Bats
Predator Killings Planned: State Game Managers Target Cougars, Coyotes
Video review: "Cleaning Skulls for Pleasure or Profit" by Ken Carver, Maplewood, MN. VHS. Approx. 120 minutes. $19.95 postpaid.
House Backs Funding of Program That Targets Predators
Armadillos Considered Delicacies By Some; Leprosy Threat by Others

Insertional Inactivation Of Treponema Denticola Tap1 Results In A Nonmotile Mutant With Elongated Flagellar Hooks, Ronald J. Limberger, Linda L. Slivienski, Jacques Izard, William A. Samsonoff

Department of Food Science and Technology: Faculty Publications

The treponemal fla operon is comprised of numerous motility-related genes; however, the initial gene of this operon, tap1, has no known function. A recently developed system to generate specific mutants in Treponema denticola was utilized to determine if Tap1 was essential for motility. T. denticola tap1 and flanking DNA were identified, cloned, and sequenced, and a suicide plasmid that contained tap1 interrupted with an erythromycin resistance cassette (ermF and ermAM) was constructed. Because of potential polar effects from this cassette, a second plasmid that contained tap1 interrupted with a modified erythromycin resistance cassette that lacked the putative ermF transcription terminator …

Child Physical Abuse, David J. Hansen, Georganna Sedlar, Jody E. Warner-Rogers

Department of Psychology: Faculty Publications

Widespread attention to the problem of child physical abuse has increased dramatically in recent decades. Extensive research evidence has described child physical abuse as a complex, multidimensional phenomenon that is best assessed by procedures using multiple modalities (e.g., interview, self-report, direct observation) that address multiple content areas. Comprehensive assessment is essential for identifying risk and occurrence of abuse, guiding the focus or direction of treatment, as well as monitoring treatment efficacy and outcome, all of which may be disseminated to interested parties as appropriate (e.g., CPS, judicial system, school, other treatment providers). Increasingly specific and relevant procedures have become available …

Locomotion And Conditioned Place Preference Produced By Acute Intravenous Amphetamine: Role Of Dopamine Receptors And Individual Differences In Amphetamine Self-Administration, M. T. Bardo, J. M. Valone, Rick A. Bevins

Department of Psychology: Faculty Publications

Although previous studies have shown that dopamine (DA) antagonists block amphetamine reward, these studies have utilized animal models that involve repeated exposures to amphetamine. The present investigation examined the effect of DA antagonists on single-trial conditioned place preference (CPP) produced by acute intravenous (IV) amphetamine in rats. In the first experiment, rats were prepared with a jugular catheter and then received an acute IV injection of amphetamine (0.1–3 mg/kg) paired with one compartment of a CPP apparatus. Relative to sham controls (no IV catheter), amphetamine produced a dose-dependent increase in locomotor activity and CPP. Two further experiments demonstrated that both …

Cynopterus Sphinx, Jay F. Storz, Thomas H. Kunz

Jay F. Storz Publications

Order Chiroptera, Family Pteropodidae, Subfamily Pteropodinae, Tribe Cynopterini, Subtribe Cynopterina, Genus Cynopterus. Five species are recognized: C. brachyotis, C. horsfieldi, C. nusatenggara, C. sphinx, and C. titthaecheileus (Koopman, 1993). A key to the species is given in Lekagul and McNeely (1977).

Who’S Buried In Custer’S Grave?, P. Willey, Douglas D. Scott

Department of Anthropology: Faculty Publications

On 10 October 1877, the year after the Battle of the Little Bighorn, General George A. Custer’s coffin was transported from a temporary grave in Poughkeepsie, NY, by steamer and cortege to permanent interment in the U.S. Military Academy’s Post Cemetery. The ceremony included the appropriate military and funerary rituals. There were, nevertheless, reasons to believe that Custer’s skeleton may not have been in the coffin—thus, he may have missed his own funeral. Custer’s remains, or part of them, may have been overlooked during the exhumation and left on the battlefield, only to be recovered around 1940. These bones, as …

Monitoring Of Transmission Of Tuberculosis Between Wild Boars And Cattle: Genotypical Analysis Of Strains By Molecular Epidemiology Techniques, Andrea Serraino, Giulia Marchetti, Valeria Sanguinetti, Maria Cristina Rossi, Renato Giulio Zanoni, Lidia Catozzi, Alessandra Bandera, Walter Dini, Walter Mignone, Fabio Franzetti, Andrea Gori

Michigan Bovine Tuberculosis Bibliography and Database

An epidemiological survey for the monitoring of bovine tuberculosis transmission was carried out in western Liguria, a region in northern Italy. Fifteen Mycobacterium bovis strains were isolated from 63 wild boar samples (62 from mandibular lymph nodes and 1 from a liver specimen). Sixteen mediastinal lymph nodes of 16 head of cattle were collected, and 15 Mycobacterium bovis strains were subsequently cultured. All M. bovisstrains isolated from cattle and wild boars were genotyped by spoligotyping and by restriction fragment length polymorphism (RFLP) analysis with the IS6110 and IS1081 probes. All M. bovis strains showed the typical spoligotype characterized by …

Canadian Cooperative Wildlife Health Centre, Volume 6-2, Winter 1999

Canadian Cooperative Wildlife Health Centre: Newsletters and Publications

New Staff Member - Quebec Regional Centre
International Cooperation in Wildlife Forensics
Road Salt and Small Birds (request for specimens)
West Nile Virus and other Zoonotic Arboviruses in North America
Canada-wide epidemic of winter tick in moose
Mortality among great black-backed and herring gulls in the Maritime provinces
Intraspecific killing in common loons
Poxvirus and multiple tumors in a grey squirrel
Circovirus infection in a rock dove
Fatal Sphaeridiotrema sp infection in lesser scaup - part II
Botulism type E in fish-eating birds, Lake Erie and Lake Huron
Gull mortality - Kitchener
Pneumonia and Septicemia in Dall's sheep, Mackenzie Mountains, …

Absence Of Kaposi's Sarcoma-Associated Herpesvirus Dna In Bacillary Angiomatosis-Peliosis Lesions, David A. Relman, David N. Fredricks, Kristine E. Yoder, Ginat Mirowski, Timothy Berger, Jane E. Koehler

U.S. Department of Veterans Affairs Staff Publications

Bartonella henselae and B. quintana induce an unusual vascular proliferative tissue response known as bacillary angiomatosis (BA) and bacillary peliosis (BP) in some human hosts. The mechanisms of Bartonella-associated vascular proliferation remain unclear. Although host factors probably play a role, microbial coinfection has not been ruled out. Because of the vascular proliferative characteristics noted in both Kaposi's sarcoma (KS) and BA and occasional colocalization of KS and BA, the possibility was explored that KS-associated herpes virus (KSHV) might be associated with BA lesions. Tissues with BA and positive and negative control tissues were tested for the presence of KSHV …

Nf99-406 Health Care Costs And Financially Troubled Times, Mary Ellen Rider

Agricultural Research Division: News and Annual Reports

This NebFact discusses issues brought up regarding health care in a challenging financial climate.

1999-00 Unopa Executive Board Minutes

UNOPA Minutes

No abstract provided.

Ec99-828 Retailing Patterns & Trends Across Nebraska, Bruce B. Johnson, Brandon G.Y. Raddatz

University of Nebraska-Lincoln Extension: Historical Materials

This report presents the retailing patterns across Nebraska over time. It assesses historical trends and current conditions. It hopes to present community and business leaders accurate and detailed information to:

a. understand the general trends underway,

b. perform relevant comparative analysis with other communities, and

c. identify strategies which may contribute to retail vitality in their areas.

Phylogenetic Systematics Of The Borophaginae (Carnivora: Canidae), Xiaoming Wang, Richard H. Tedford, Beryl E. Taylor

University of Nebraska State Museum: Mammalogy Papers

The subfamily Borophaginae (Canidae, Carnivora, Mammalia) was erected by G. G. Simpson in 1945 to include seven genera of large, bonecrushing ‘‘dogs’’ in the late Tertiary of the northern continents. As a monophyletic group of canids, the Borophaginae is now known to be much more diverse than was originally envisioned but is confined within the middle to late Tertiary of North America. Fossil records of the borophagines are well represented and members of this prolific clade are often the most common predators in the late Tertiary deposits. Largely due to the Childs Frick Collection at the American Museum of Natural …

Tertiary Bats (Mammalia: Chiroptera) From Northern Nebraska, Nicholas J. Czaplewski, Bruce E. Bailey, Richard G. Corner

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Scarce remains of bats are reported from five localities in northern Nebraska in which other kinds of vertebrates are much more common. ?Oligomyotis or ?Myotis, possibly of an undescribed species, is represented by fragments of jaws and humeri from an early Arikareean (late Oligocene) locality in Dawes County. Several toothless jaw fragments from the late Hemingfordian (middle Miocene) Companion Quarry in Sioux County represent an indeterminate microchiropteran. An indeterminate species of Myotis was encountered in the middle Clarendonian (late Miocene) Ashfall site in Antelope County. A hairy-tailed bat, Lasiurus sp. indet., occurred in late Clarendonian (late Miocene) …

Transactions Of The Nebraska Academy Of Sciences Table Of Contents: Volume 25 (1999)

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Editorial Contents

Nebraska Academy of Sciences Officers, Policy Committee ................. ii

Editorial Board ................. iv

Membership Objectives and Friends of the Academy ................. v

Nebraska Association of Teachers of Science ................. vi

Preparation of manuscripts ................. inside back cover

Papers

Using omissive faults to obtain local convergence in partially connected networks (M. H. Azadmanesh and A. W. Krings) ................. 1

New floristic records for Nebraska-5 (Steven B. Rolfsmeier, Robert F. Steinauer, and David M. Sutherland) ................. 15

A key to dicotyledonous rosettes of eastern Nebraska (Phillip D. Moore) ................. 23

The brine shrimp Artemia franciscana in closed microalgal-based microcosms (biospheres) …