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Development Of Methodology For Raman Microspectroscopic Analysis Of Oral Exfoliated Cells, Hugh Byrne, Fiona Lyng, Alison Malkin, Ola Ibrahim, Genecy Calado, Steven Flint, Isha Behl
Development Of Methodology For Raman Microspectroscopic Analysis Of Oral Exfoliated Cells, Hugh Byrne, Fiona Lyng, Alison Malkin, Ola Ibrahim, Genecy Calado, Steven Flint, Isha Behl
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Oral squamous cell carcinoma ranks as the 15th most common cancer worldwide. The present study was undertaken to standardise a protocol for the analysis of oral exfoliated cells using Raman microspectroscopy. For this purpose, samples were obtained from two different sites, based on prevalence of disease (ventral side of the tongue and buccal mucosa). Different oral rinsing agents were employed and it was concluded that non-alcoholic mouthwash adequately removes food debris. Samples were collected using various collection tools and compared. It was observed that endo-cervical brushes yielded cells from deeper layers of the epithelium. Furthermore, monolayer formation of cells was …