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A Tissue Culture Perfusion Chamber With A Substratum Of Reconstituted Collagen, Thomas M. Harris

MCV/Q, Medical College of Virginia Quarterly

A tissue culture chamber is described that introduces the concept of a fixed platform within a "perfusion type" chamber. The platform is made of stainless steel mesh covered with a membrane of reconstituted collagen. The collagen membrane serves as the supporting substratum for the cultured tissue and is of such an optical quality as to permit phase-contrast microscopic observation of the tissue. The perfusion chamber is a Sykes-Moore tissue culture chamber modified to twice its normal depth to receive the screen. Cell growth in the experimental chamber is superior to growth in the control chamber both in speed of proliferation …