Digital Commons Network^™

Antibiotic Use By Members Of The American Association Of Endodontics: A National Survey For 2009- A Follow Up From The Report In 1999, Pye Kyu

Theses and Dissertations

The purpose of this study was to determine the changes in prescribing habits of active members of American Association of Endodontics (AAE) with regards to antibiotics in comparison to the findings reported by Yingling et al. in 1999. The invitations to take the online survey were sent via email to 2593 active members. A response rate of 37.75% was obtained. It was determined to be adequate for analysis and for comparison to the results obtained by Yingling et al. Comparisons between the percentages shown in this survey and the previous survey were tested using a z-test. An ANOVA model was …

Retinoic Acid Induced 1 Gene Analysis In Humans And Zebrafish, Bijal Vyas

Theses and Dissertations

Smith-Magenis syndrome (SMS) is a complex mental retardation syndrome caused by deletion of 17p11.2 region or mutation of the RAI1 gene (retinoic acid induced 1). Individuals with SMS typically exhibit speech and motor delays, mental retardation, characteristic craniofacial and skeletal anomalies, and a distinct neurobehavioral phenotype that includes sleep disturbances, stereotypes, and maladaptive and self-injurious behaviors. RAI1 is thought to be a transcription factor modulating the expression of genes involved in a variety of cellular functions. Previous studies have shown the RAI1 gene being induced by retinoic acid (RA), a derivative of vitamin A. RA plays a significant role in …

Heritability Of Autoantibody Levels In A Twin Population, Amal Rastogi

Theses and Dissertations

AIM: This study aims to determine what portion of specific autoantibody phenotypes are genetically determined by using a twin model. METHODS: This study specifically examines Anti-Ro(SSA), Anti-La (SSB), Anti-Sn/RNP, Anti-Sm, Anti-Jo-1, Anti-Scl-70, Anti-Tg & Anti-TPO, Anti-dsDNA, Anti-PS, and Anti-cardiolipin antibodies for their heritability. This study examined 104 same-sex adult twins (66 monozygous, 38 dizygous) for the above mentioned autoantibody values. The serum autoantibody values in each subject were quantified using automated ELISA. Descriptive statistics including, distributions, quantiles, and moments were calculated by zygosity for continuous antibody values, subject ages, gender, race and smoking status. Categorical antibody levels were used to …

Prevalence Of White Spot Lesions During Orthodontic Treatment, Julian Dixon

Theses and Dissertations

The reported prevalence of decalcification in orthodontic patients varies from 2 to 96% mainly due to the lack of a standard examination technique. The aims of this study were: 1) to determine the prevalence of white spot lesions around brackets using visual examination and the DIAGNOdent; 2) to determine which teeth were the most susceptible to decalcification; and 3) to test the accuracy of the DIAGNOdent by comparing to the visual examination. The presence of white spot lesions was determined in two groups of patients who were 6 and 12 months into orthodontic treatment, respectively. The control group consisted of …

Frequency Of Tlr-2, 4, 9 And Cd14 Polymorphisms In Aggressive Periodontitis Population In African-Americans, Melanie Chou

Theses and Dissertations

Aim: The aim of this study is to determine the frequency of single nucleotide polymorphisms (SNPs) in various pattern recognition receptor (PRR) genes, including Toll like receptors (TLR) -2, -4, -9, and CD14 in chronic (CP), localized (LAP) and generalized aggressive (GAP) periodontitis and periodontally healthy (NP) patients in an African American population. Methods: A total of 205 subjects were involved in the study. The LAP group consists of 25 subjects, the GAP group 50 subjects, the CP group 73 subjects and the NP group 57subjects. Genotyping was performed in TLR2 (G2408A), TLR4 (A896G),TLR9 (T1486C) and CD14 (C260T) genes by …

The Effect Of Single Nucleotide Polymorphisms (Snps) In Toll-Like Receptors -2, -4, -9, And Cd14 Genes In An African-American Population With Chronic Periodontitis, Willard Maughan

Theses and Dissertations

AIM: to determine if a relationship exists between TLR-2, TLR-4, TLR-9, or CD14 polymorphisms and risk for developing chronic periodontal disease in an African-American population. This is the first study conducted to determine role of SNPs in TLR genes and CD14 gene in a periodontally-diseased African-American population. Additionally, this is the first study to assess the role of TLR-9 polymorphism in periodontitis patients. METHODS: A total of 130 subjects were involved in the study. The chronic periodontitis (CP) group contained 73 subjects, and the healthy control (NP) group 57subjects. Genotyping was performed in TLR2 (G2408A), TLR4 (A896G),TLR9 (T1486C) and CD14 …

Perceived Responsibility For The Development Of White Spot Lesions During Orthodontic Treatment, Blake Maxfield

Theses and Dissertations

White spot lesions (WSLs) or decalcifications remain a common complication in orthodontic patients with poor oral hygiene. The purpose of this study was to compare attitudes regarding the development of WSLs among patients, parents, orthodontists and general dentists and improve prevention and treatment protocols through better communication. A survey was developed to evaluate and compare the current opinions of orthodontic patients (n=315), parents (n=279), orthodontists (n=305) and general dentists (n=191) regarding the significance, prevention and treatment of WSLs. All four groups indicated that WSLs did detract from the overall appearance of straight teeth. All four groups indicated that patients were …

Leadership And Dental Aesthetics Of Adults As Perceived By Laypersons, E. Britton Montgomery

Theses and Dissertations

The purpose of this study was to determine if dental aesthetics is perceived by adults to affect an individual’s ability to emerge as a leader. An electronic survey was constructed using facial frontal smiling photographs of 10 patients. Statements were formulated to evaluate four leadership characteristics: intelligence, self-confidence, friendliness, and trustworthiness. Evaluators indicated their level of agreement with each statement for each subject using a 100mm Visual Analog Scale (VAS). Data was obtained from 214 evaluators. Picture ratings were analyzed using repeated-measures mixed-model analyses. Evaluator factors and picture factors were considered when testing for crooked vs. straight teeth differences. There …

Determinants Of Hepatitis B Vaccination Among Adults In The United States: Nhanes 1999-2006, Conschetta Wright

Theses and Dissertations

Purpose: The primary objective of this study was to estimate the prevalence of vaccination and HBV infection status of adults and to evaluate the trend in self reported vaccination and seroprevalence for Hepatitis B for this population. Additionally, this study sought to assess the association between vaccination rates, seroprevalence (HBsAg, anti-HBc, and anti-HBs), demographic (age, gender, location of birth, race/ethnicity), and socioeconomic (annual household income, education level, insurance coverage and access to care, marital status) characteristics. Methods: Eight years, 1999-2006, of the National Health and Nutrition Examination Survey (NHANES) data were used. NHANES participants aged 20-59 years who contributed data …

Delayed And Forgone Care For Children With Special Health Care Needs In Virginia: A Cross-Sectional Study Using 2005-2006 Ns-Cshcn, Nevena Skoro

Theses and Dissertations

Objectives The objectives of our study were to: 1) summarize illness characteristics and functional ability difficulties among Children With Special Health Care Needs (CSHCN) in Virginia, 2) examine the distribution of socio-demographic, health insurance, and health-related factors among Virginia’s CSHCN, 3) quantify the influence of these socio-demographic, health insurance, and health-related factors on delayed and forgone care of CSHNC in Virginia, and 4) analyze reasons for delayed and forgone care among Virginia’s CSHCN. Methods Data from the 2005-2006 National Survey of Children with Special Health Care Needs (NS-CSHCN) were used for this study. Three categories of predictor variables were studied: …

Functional Health Literacy And The Use Of Dental Services In Young Children, Melissa Fries

Theses and Dissertations

Purpose: The purpose of this study is to examine parental Functional Health Literacy and their child’s subsequent utilization of dental services. Methods: This was a prospective cohort study of children (n=1175) enrolled in the Child Health Investment Partnership of Virginia (CHIP). Descriptive statistics and separate multivariate logistic regressions were used to determine the relationship between functional health literacy measures; 1) Health Care Literacy (HCL), 2) Personal Health Literacy (PHL), and 3) LSP 22 scale, with utilization as measured as number of dental visit/s. Results: Descriptive analysis of the cohort reveals: 45% black, 40% white, 10% Hispanic, 5% other, 41% of …

The Evaluation Of Pain Experienced By Children Undergoing Simple Extraction Using 2% Lidocaine Versus Oraqix Topical Anesthetic Gel, John Haffner

Theses and Dissertations

Purpose: The purpose of this study is to examine the pain response experienced by children undergoing simple tooth extractions using 2% Lidocaine injection versus Oraqix topical anesthetic gel. Methods: This study is being conducted at VCU pediatric dentistry clinic. The sample size will consist of 15 children ages 7-12 undergoing a simple extraction procedure. Each participant is randomly assigned to one of two groups, the lidocaine injection group or the Oraqix topical group. The pain level will be measured at four key events during the procedure. The first event will evaluate the pain at baseline. The second event will measure …

Obesity, Adiposity, And Satiety In Mouse Models Of Smith-Magenis Syndrome And Dup(17)(P11.2) Syndrome, Brooke Burns

Theses and Dissertations

Smith-Magenis syndrome (SMS) is a complex disorder caused by haploinsufficiency of RAI1 and characterized by sleep disturbances, behavioral abnormalities, mental retardation, and obesity in teens and adults. Rai1+/- mice are obese after 20 weeks. Dup(17)(p11.2) syndrome is a complex disorder associated with overexpression of RAI1. A transgenic mouse model of dup(17)(p11.2) syndrome overexpresses Rai1 and results in a mouse that is growth delayed. In order to characterize the obese phenotypes of mouse models of SMS and the role of RAI1 in obesity, daily food intake and serum levels of insulin, glucose, PPY, and leptin were measured; adiposity was studied by …

Comparing Cyclic Fatigue Of The New Gt® Series X(Tm) Files To The Endo Sequence™ Rotary Instruments, Nathan Wayment

Theses and Dissertations

The purpose of this study was to examine the number of rotations to failure of two different rotary file systems, EndoSquence Brassler USA (Savannah, GA) and GT(TM) series X(TM). Files sizes 20,30,40 with 0.04, 0.06 tapers of GT series X and Endo Sequence files size and taper used were 20, 30, 40 with 0.04 and 0.06 taper. Like tip and tapers were compared between systems. All files tested were 25mm in length. Files were allocated into 12 groups of 10 files each, and mounted to a universal testing machine. Each file was rotated at 300 rpms until fractured occurred. The …

Comparative Analysis Of Torsional Strength Between Profile® Gt® And Gt® Series X(Tm) Nickel Titanium Rotary Instruments, Jake Reynolds

Theses and Dissertations

The purpose of this study was to compare the resistance to fracture by the twisting of two nickel titanium (NiTi) rotary file systems. The Profile® GT® (GT) and the GT® Series X(TM) (GTX) files were tested to analyze the maximum torsional strength and the maximum angular deflection at the time of separation of the files. For each system, ten new files were tested at each of the following eight taper/size designations for a total of 160 files: 20/0.04, 20/0.06, 30/0.04, 30/0.06, 30/0.08, 40/0.04, 40/0.06, and 40/0.08. The American National Standards Institute / American Dental Association Specification No. 28 was implemented …

Comparing Cyclic Fatigue Of The New Gt® Series Xtm Files To The Original Profile® Gt® Rotary Instruments, Steven Osmond

Theses and Dissertations

The purpose of this study was to test the number of rotations to fracture of the M-Wire GT® Series X(TM) rotary files compared to the original ProFile® GT® rotary files in a simulated curved canal. Eighty GT® Series X(TM) files of 25mm length were divided into eight groups of ten, one for each of the new GT® Series X(TM) files. Eighty original ProFile® GT® files of 25mm length were divided into eight groups of the same tip and taper sizes consistent with the GT® Series X(TM) file groups. Files were rotated at 300 rpm. While the angle may have slightly …

Genetic Variations Of Cyp2b6 Enzyme And The Response To Meperidine In Oral Sedation, Sally Hua

Theses and Dissertations

Purpose: The purpose of this study was to determine the relationship of CYP2B6 genotype to the clinical response to meperidine in pediatric dental patients. Methods: Twenty-five patients, ASA I/ II, 45–92 months old, received an oral sedative regimen containing meperidine for dental treatment. The North Carolina Behavior Rating Scale (NCBRS) and Overall Effectiveness of Sedation Scale (OESS) were used to assess their behavior and sedation outcome. Saliva DNA samples were genotyped by PCR-RFLP. Results: We found the following genotype distributions: homozygous wild-type 1*1 (n = 8, 32%), heterozygous 1*6 (n = 13, 52%), and homozygous variant 6*6 (n = 4, …

Preventive Dental Services For Infants And Subsequent Utilization Of Dental Services, Elizabeth Berry

Theses and Dissertations

The purpose of this study was to examine the use of dental services for young children following a preventive oral health intervention in a pediatric medical clinic. Over a 3 year period (2005-2008), children 0-36 months of age, enrolled in Medicaid, were provided preventive oral health services in a medical setting. Descriptive statistics and multivariate logistic regression were used to determine the effect receiving the preventive oral health services in a medical setting with the outcomes of dental utilization. 15% were determined to have dental caries at the intervention and 42% found to have a dental visit post-intervention. Children determined …

The Effects Of Motivational Interviewing In Pregnancy On Infant Oral Health Knowledge And Behavior, Patricia Arteaga

Theses and Dissertations

Purpose: The purpose of this study is to examine infant oral health knowledge and readiness of expectant mothers to incorporate preventive oral health behaviors for their infants. Methods: The study used a prospective cohort design to examine the effect of prenatal education and motivational counseling with expectant mothers about infant oral health care readiness. Participants were all enrolled in a prenatal care program called Centering Pregnancy offered by VCU’s Department of Obstetrics and Gynecology. They all completed a pre-survey questionnaire, followed by a motivational counseling intervention, and then completed a post-survey questionnaire. Results: A sample of 30 pregnant women completed …

Bonding Orthodontic Brackets To Stainless Steel Crowns, Daniel Stewart

Theses and Dissertations

The purpose of this study was to compare shear bond strengths of brackets bonded to stainless steel crowns using various orthodontic adhesives and surface conditioning techniques. One hundred and twenty mandibular first molar stainless steel crowns were randomly divided into groups: (1): Aluminablasting + Metal Primer + Assure; (2): Aluminablasting + Silane Coupling Agent + Transbond; (3): Diamond Bur Abrasion + Metal Primer + Assure; (4): Diamond Bur Abrasion + Silane Coupling Agent + Transbond; (5: control): Acid Etching + Metal Primer + Assure; (6: control): Acid Etching + Silane Coupling Agent + Transbond. Bond strength was tested using a …

Dental Anatomy Carving Computer-Assisted Instruction Program: An Assessment Of Student Performance And Perceptions, Elizabeth T. Nance, Sharon K. Lanning, John C. Gunsolley

Periodontics Publications

The purpose of this study was to compare the performance of students exposed to two different instructional modalities for dental anatomy wax carving: CAI (computer-assisted instruction) using DVD technology, or traditional laboratory instruction. Students’ self-assessment scores were also compared to faculty scores, and students’ perceptions of their teaching modality were analyzed. Seventy-three first-year dental students (response rate 81 percent) participated in this randomized single blind trial, in which faculty graders were blinded to student group assignment. There were no statistical differences, as determined by the Wilcoxon non-parametric test and a t-test, between the faculty grades on the wax carving from …

Sleep And Sleep Disorders In Later Life, Robert D. Vorona

Case Studies from Age in Action

Educational Objectives

1. Describe basic changes in sleep that occur as we age.

2. Review several important sleep disorders common in later life

3. Discuss treatment modalities for these sleep disorders and potential benefits of therapy.

Apoptotic Engulfment Pathway And Schizophrenia, Xiangning Chen, Cuie Sun, Qi Chen, F. Anthony O'Neill, Dermot Walsh, Ayman H. Fanous, Kodavali V. Chowdari, Vishwajit L. Nimgaonkar, Adrian Scott, Sibylle G. Schwab, Dieter B. Wildenauer, Ronglin Che, Wei Tang, Yongyong Shi, Lin He, Xiong-Jian Luo, Bing Su, Todd L. Edwards, Zhongming Zhao, Kenneth S. Kendler

Human and Molecular Genetics Publications

Background

Apoptosis has been speculated to be involved in schizophrenia. In a previously study, we reported the association of the MEGF10 gene with the disease. In this study, we followed the apoptotic engulfment pathway involving the MEGF10, GULP1, ABCA1 and ABCA7 genes and tested their association with the disease.

Methodology/Principal Findings

Ten, eleven and five SNPs were genotyped in the GULP1, ABCA1 and ABCA7 genes respectively for the ISHDSF and ICCSS samples. In all 3 genes, we observed nominally significant associations. Rs2004888 at GULP1 was significant in both ISHDSF and ICCSS samples (p = 0.0083 and …

The Servant Leader: A Higher Calling For Dental Professionals, Fred Certosimo

General Practice Publications

The dental profession is guided by normative principles that provide guidance to our leaders and practicing dentists in addressing the needs of patients and the profession, yet there is room for incorporating new ideas that help dental professionals meet their professional obligations. The purpose of this essay is to discuss the concept of “servant leadership,” especially in contrast with “self-serving leaders,” and to suggest that servant leadership is consistent with the high ethical and professional ideals of the dental profession. The servant leader is the antithesis of the self-serving leader, who incessantly seeks more power and acquisition of material possessions. …

Diagnosis Of Infections Caused By Pathogenic Free-Living Amoebae, Bruno Da Rocha-Azevedo, Herbert B. Tanowitz, Francine Marciano-Cabral

Microbiology and Immunology Publications

Naegleria fowleri, Acanthamoeba spp., Balamuthia mandrillaris, and Sappinia sp. are pathogenic free-living amoebae. N. fowleri causes Primary Amoebic Meningoencephalitis, a rapidly fatal disease of the central nervous system, whileAcanthamoeba spp. and B. mandrillaris cause chronic granulomatous encephalitis.Acanthamoeba spp. also can cause cutaneous lesions and Amoebic Keratitis, a sight-threatening infection of the cornea that is associated with contact lens use or corneal trauma. Sappinia pedata has been identified as the cause of a nonlethal case of amoebic encephalitis. In view of the potential health consequences due to infection with these amoebae, rapid diagnosis is critical for early treatment. Microscopic examination and …

Virginia Dental Journal (Vol. 86, No. 1, 2009)

Virginia Dental Journal

No abstract provided.

Virginia Dental Journal (Vol. 86, No. 2, 2009)

Virginia Dental Journal

No abstract provided.

Virginia Dental Journal (Vol. 86, No. 3, 2009)

Virginia Dental Journal

No abstract provided.

Virginia Dental Journal (Vol. 86, No. 4, 2009)

Virginia Dental Journal

No abstract provided.

Identification And Characterization Of Prevotella Intermedia 17 Toll-Like Receptor 2 Ligands, Jamie Feng-Hsia Yuan

Theses and Dissertations

Periodontal disease is one of the most prevalent human diseases affecting millions of individuals. Bacteria are the trigger of the host response that then results in initiation and progression of periodontal disease. The interactions between the host and the bacteria, although very complex and intricate, are not well understood. Species of gram-negative anaerobic bacteria implicated in the development of periodontal disease include Porphyromonas gingivalis and Prevotella intermedia. The goal of our study was to identify P. intermedia surface proteins interacting with TLR2 receptor. Using Cy5-labeled surface proteins we identified multiple proteins using our capture assay. The interaction of three …