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Cellular Coping Mechanisms To Hypoxia In The Longhorn Sculpin (Myoxocephalus Octodecemspinosus) Brain, Kelly Heino

College of Graduate Studies: Theses & Dissertations

The loss of neurological function due to hypoxia remains to be a challenge in many species due to the lack of knowledge and understanding of cellular responses. To investigate cellular responses and survival strategies of the brain during hypoxia and post-hypoxia recovery in a moderate hypoxia-tolerant species, with the hopes of identifying possible therapeutic remedies, thirty Longhorn sculpin, Myoxocephalus octodecimspinosus, were challenged with acute hypoxia (40% O2 saturation for 1 h) and twelve additional sculpin were used as controls (~90% O₂ saturation). Fish were sampled at each of the following time points: control/normoxic conditions (T=0 h); hypoxic conditions …

Molecular And Immunohistochemical Identification Of A Sodium Hydrogen Exchanger-2c (Nhe2c) Paralog In The Gills Of Marine Longhorn Sculpin (Myoxocephalus Octodecemspinosus), Demi Brett Rabeneck

College of Graduate Studies: Theses & Dissertations

Sodium hydrogen exchanger proteins (NHEs) are members of the cation proton antiporter superfamily (CPA) and are thought to function in fish for maintaining physiological ion concentrations and acid-base balances by excreting excess H+ ions from the body in exchange for Na+ ions. There are many more types of these proteins in teleost fish than in mammals due to putative genome duplication. This study describes a new form of NHE2 in the gills of marine longhorn sculpin, Myoxocephalus octodecemspinosus, designated NHE2c. Sequencing revealed that the NHE2c nucleotide-coding region transcribes a peptide 795 amino acids in length with an estimated molecular weight …

Rh Glycoprotein As An Ammonia Transport Molecule, Matthew B. Phillips

College of Graduate Studies: Theses & Dissertations

Fish use their gills to excrete ammonia in order to eliminate nitrogenous waste. We hypothesize that this mechanism is accomplished by one or more transport proteins in the Rh glycoprotein (RhxG) family. Longhorn sculpin (Myoxocephalus octodecemspinosus) cDNA was amplified using polymerase chain reaction (PCR) and then the PCR products were visualized on an agarose gel. The cDNA from the gel bands was then sequenced and the gene sequence fragments were assembled and completed by rapid amplification of the cDNA ends (RACE). By this process we have obtained large portions of the gene sequences of the four known paralogues located in …

Aquaporin 3 Water Channel Protein Gene Expression In Squalis Acanthias Dogfish Shark, Debra Lee Murray

College of Graduate Studies: Theses & Dissertations

Aquaporins are a family of membrane proteins that provide channels for the rapid movement of water molecules and some small solutes across the cellular membrane. Since the discovery of the first water channel protein, hundreds of homologous proteins have been found in all forms of life and much has been learned about their complex role in body water homeostasis. This is the first study to use molecular and immunological techniques to demonstrate that an orthologue of mammalian AQP3 is present in the epithelial cells of Squalus acanthias dogfish shark kidney, gills, rectal gland, esophagus, stomach, eye and brain. The full-length …

Characterization Of Na+/H+Exchanger-3 (Nhe3) In The Gills Of Longhorn Sculpin (Myoxocephalus Octodecemspinosus), Curtis Eugene Lanier

College of Graduate Studies: Theses & Dissertations

Acid-base regulation is a vital mechanism in homeostasis. Fish must maintain optimal physiological acid-base parameters during changes in an aquatic habitat. Environmental factors such as changes in salinity affect fish ability to maintain optimal blood plasma ion concentrations. Ion regulation influences the fish ability to maintain optimal acid-base balance. The Na+/H+ Exchanger-3 (NHE-3) has been shown to be the primary membrane ion transporter responsible for acid-base balance in the mammalian kidney. The objective of this study is to characterize NHE-3 in the gills of longhorn sculpin (Myoxocephalus octodecimspinosus).

Cloning And Sequencing Of Sodium-Hydrogen Exchanger In The Gills Of The Long-Horned Sculpin (Myoxocephalus Octodecimspinosus), Danielle Lee Gunning

College of Graduate Studies: Theses & Dissertations (1964–2006)

No abstract provided.

Acid-Base Regulation In Three Marine Teleosts: The Oyster Toadfish (Opsanus Tau), The Winter Flounder (Pseudopleuronectes Americanus), And The Long-Horned Sculpin (Myoxocephalus Octodecimspinosus), Dana Compton Mccullough

College of Graduate Studies: Theses & Dissertations (1964–2006)

In this study, three species of marine fish were exposed to a variety of dilute salinities in order to determine what effects exposure to diluted seawater may have on acid-base and ion balance. The oyster toadfish (Opsanus tau) and the winter flounder (Pseudopleuronectes americanus) are considered to be able to live in a broad range of salinities, euryhaline (Evans, 1979). The long-homed sculpin, Myoxocephalus octodecimspinosus, is considered to be a stenohaline fish, not able to withstand drastic changes in ambient salinity (Claibome and Evans, 1988).

Initially, toadfish exposed to 20 mM diluted seawater took up …