Digital Commons Network^™

Brain On Drugs: The Rewarding And Punishing Effects Of Addiction And Relapse, Abby Ferrell

Undergraduate Honors Theses

Addiction is a multidimensional disorder that is heavily influenced by behavior, biology, and social interaction. These factors work together to make addiction persist and make relapse easier. Three major models of addiction are explored--behaviorism, biology, and social learning theory, which all contribute to the understanding of addiction, and their respective treatments can be individual, and when integrated. Behavior models highlight how conditioning and reinforcement contribute to addiction, while biological studies focus on dopamine changes in the brain that alter other areas of brain chemistry. Social learning theories add to the environmental cue associations that behaviorism mentions briefly. The implications that …

Structural Biology Of Mcr-1-Mediated Resistance To Polymyxin Antibiotics, Isabel Cristina Materon, Timothy Palzkill

Faculty, Staff and Students Publications

Polymyxins, a last resort antibiotic, target the outer membrane of pathogens and are used to address the increasing prevalence of multidrug-resistant Gram-negative bacteria. The plasmid-encoded enzyme MCR-1 confers polymyxin resistance to bacteria by modifying the outer membrane. Transferable resistance to polymyxins is a major concern; therefore, MCR-1 is an important drug target. In this review, we discuss recent structural and mechanistic aspects of MCR-1 function, its variants and homologs, and how they are relevant to polymyxin resistance. Specifically, we discuss work on polymyxin-mediated disruption of the outer and inner membranes, computational studies on the catalytic mechanism of MCR-1, mutagenesis and …

Targeting Energy Intake And Circadian Biology To Engage Mechanisms Of Aging In Older Adults With Obesity: Calorie Restriction And Time-Restricted Eating, Satchidananda Panda, Geraldine Maier, Dennis T Villareal

Faculty, Staff and Students Publications

With the rise in obesity across age groups, it has been a hindrance to engaging in physical activity and mobility in older adults. Daily calorie restriction (CR) up to 25% has been the cornerstone of obesity management even though the safety in older adults remains incompletely understood. Although some adults can follow CR with clinically significant weight loss and improved health metrics, CR faces 2 obstacles-many fail to adopt CR and even among those who can adopt it short term, long-term compliance can be difficult. Furthermore, there is a continuing debate about the net benefits of CR-induced weight loss in …

Exploring How Advanced-Level Biology Teachers Integrate Ict In Teaching And Learning,A Case Of One Public Secondary School In Songwe Region, Tanzania, Rose John Shiyo

Master of Education

Information and Communications Technology (ICT) has pedagogical advantages that can help teachers design, teach, and assess biology lessons. The aim of this study was to explore how teachers integrate ICT during the teaching of Biology at A-level classes in Songwe region, Southern Tanzania. The research site of this study is one public advanced-level secondary school in the Mbozi district. The purposive sampling technique was used to get the sample comprising two, A-level biology teachers, one ICT technician, one laboratory technician, and six students from A-level classes who were persuing a science subject combination known as CBG (Chemistry Biology Geography). This …

Gene Vectors With Fluorescence Tracking Capabilities, Sophia Despina Angelopoulos

Browse all Theses and Dissertations

This project focuses on the optimization of benzothiazole-based chromophores to utilize them as fluorescent tags functionalized onto stimuli-responsive, or “smart,” polymers as non-viral gene delivery vectors for gene therapy applications. Blue-fluorescent emissive chromophores will allow tracking capabilities for the transfection pathway of the vector to be monitored as it delivers the DNA payload within intercellular space. The most appropriate chromophore must be covalently bound to a free amine within the vector, which is accomplished through NAS chemistry from fluorophenyl-benzothiazoles (F-BTZ-CBz) derivatives to amidated hyperbranched poly(ethyleneimine)(HPEI-IBAm0.61). HPEI is considered to be a synthetic polycation, which promotes tunable solubility through characteristics of …

The Impact Of Using An Online Learning Management System On Student Biology Achievement In A Hispanic Rural High School, Brent J. Burkott

Theses and Dissertations

The purpose for this study was to determine the effect of using online Learning Management Systems with a Blended Learning pedagogy on state assessment achievement of high school biology students with interactions analyzed based on teaching method (Face-to-Face/Blended Learning), course type (Honors/Regular) biological sex (Male/Female), risk factor (At-Risk/Not At-Risk), and socioeconomic status (High/Low). The research questions were: (1) What is the difference in achievement scores between students taught in a Blended Learning environment with a Learning Management System verses a traditional Face-to-Face learning environment, and (2) What differences in achievement exist on the Texas assessment based on teaching method, course …

Tracking Single Cell Lineages In Saccharomyces Cerevisiae, Guste Urbonaite

Yale Graduate School of Arts and Sciences Dissertations

The ability to measure the number of gene-specific mRNA molecules in individual mammalian cells has transformed the transcriptomics field. Among the key technologies enabling single-cell mRNA sequencing has been Droplet Sequencing (Drop-Seq). While this method works efficiently for mammalian cells, its direct application to yeast cells has been problematic due to cell-type specific differences such as size, doublet formation rate, and cell wall. Here we introduce yeast DropSeq, a single-cell RNA sequencing method for the study of transcriptomics in yeast. I modified and optimized the original Drop-Seq method to address the issues that emerged from smaller cell sizes and the …

04. Chemistry, Southwestern Oklahoma State University

Oklahoma Research Day Abstracts

No abstract provided.

The Bioethical Significance Of “The Origin Of Man’S Ethical Behavior” (October 1941, Unpublished) By Ernest Everett Just And Hedwig Anna Schnetzler Just, Theodore Walker Jr.

Journal of the South Carolina Academy of Science

Abstract –

E. E. Just (1883-1941) is an acknowledged “pioneer” in cell biology, and he is perhaps the pioneer in study of egg cell fertilization. Here we discover that Just also made pioneering contributions to general biology and evolutionary bioethics.

Within Just’s published contributions to observational cell biology, there are substantial fragments of his theory of ethical behavior, a theory with roots in cell biology. In addition to such previously available fragments, Just’s fully developed theory is now available. This recently discovered unpublished book-length manuscript argues for the biological origins of ethical behavior (evolving from cells to humans, within a …

9th Annual Postdoctoral Science Symposium, University Of Texas Md Anderson Cancer Center Postdoctoral Association

Annual Postdoctoral Science Symposium Abstracts

The mission of the Annual Postdoctoral Science Symposium (APSS) is to provide a platform for talented postdoctoral fellows throughout the Texas Medical Center to present their work to a wider audience. The MD Anderson Postdoctoral Association convened its inaugural Annual Postdoctoral Science Symposium (APSS) on August 4, 2011.

The APSS provides a professional venue for postdoctoral scientists to develop, clarify, and refine their research as a result of formal reviews and critiques of faculty and other postdoctoral scientists. Additionally, attendees discuss current research on a broad range of subjects while promoting academic interactions and enrichment and developing new collaborations.

The Impact Of Undergraduate Research Experiences On The Development Of Biology Students’ Domain Knowledge, Domain Interest, And Career Aspirations, Amy Haddock Keagy

UNF Graduate Theses and Dissertations

Federal and state agencies in the United States have pressured institutions in higher education to increase the number of graduates in STEM disciplines and supply an educated workforce for the increasing shortages in the STEM economy. Undergraduate research experience is one potential mechanism for supporting retention and student success within STEM disciplines. Most evaluations of the impact of undergraduate research to this point have been qualitative research studies. The purpose of this study was to use a quantitative model to examine domain knowledge, domain interest, and career aspirations in undergraduate biology majors and how participation in research experiences may impact …

Biosocial Criminology Versus The Constitution, Karen E. Balter

Regis University Student Publications (comprehensive collection)

The continually emerging field of biosocial criminology provides a basis for productively merging biology with sociological reasonings for criminal behavior. Mainstream research in criminology focuses on environmental factors as the sole reason individuals exhibit antisocial behavior patterns and may ultimately commit crimes. Criminological research has travelled in this direction for decades. The current climate within this community subscribes heavily to the notion that biology has very little to do with why people behave the way they do, and if it did, government control would be the norm. The nature of biocriminology opens a door through which constitutional issues may enter. …

Pore Water Salinity Drives Brackish Vegetation Change In Salt Marsh Tidal Restoration, Shri Andrea L. Verrill Ms

All Student Scholarship

Salt marsh vegetation plays an important role in building and maintaining elevation on the marsh surface. For marshes to persist as sea-level-rise (SLR) occurs, the marsh accretion process must increase elevation at a rate greater than or equal to the rate of SLR. If salt marshes do not maintain elevation above sea level, salt marshes will drown, and associated benefits to humans, fish, and wildlife, and their contribution to the health of estuarine and marine ecosystems will be lost. Salt tolerant plant species are adapted to frequent and prolonged periods of inundation with seawater and typically maintain elevation where they …

Studies On Development In Euphilomedes Ostracods: Embryology, Nervous System Development, And The Genetics Of Sexually Dimorphic Eye Development, Kristina Koyama

University of the Pacific Theses and Dissertations

Model organism studies have been fundamental in understanding evolutionary and developmental biology. However, non-model organisms present opportunities to study unique characteristics and as comparisons to model organisms, leading us toward broader and more relevant perspectives on diversity. The Euphilomedes genus of ostracods is an example of a non-model group with potential for evolutionary and developmental studies.

Ostracoda is an ancient, basally branching lineage of Crustaceans with a complete and prodigious fossil record. Despite the group’s promise for evolutionary studies, much remains unknown about the basic biology of this clade. There are a limited number of embryogenesis studies in Ostracoda; here, …

Evaluating College Biology Laboratory Accommodations For Students With Blindness And Visual Impairments, Barbara Rae Heard

All Theses And Dissertations

Studies show that active participation in science laboratory activities promotes student learning. However, students with blindness and visual impairments (BVI) often confront obstacles to active participation in the required activities of the college biology laboratory. Legislation requires institutions of higher education to provide accommodations for students with disabilities, yet the institutions must also maintain the academic integrity of their courses and programs. While college biology instructors provide specific accommodations, such as tactile models and audible devices, to enable active participation by students with BVI, they do so without research-supported guidelines for best practices. This mixed methods study sought to evaluate …

The Confluence Of Philosophy And Biology: An Excavation Of Philosophical Issues In Molecular And Developmental Biology, Patrick Johnson Mendie, Emmanuel Bassey Eyo (Ph.D)

Journal of Health Ethics

Philosophical evaluations have played an influential role in the growth and development of molecular and developmental biology to ensure that every individual is born healthy, born wanted and has the privilege to fulfil his or her potentials for a life free from disease and disability. This is why it becomes necessary for biologists to carefully understand human genes, evolution, cells and general human anatomy to fulfil this project. During this process, they are faced with challenges where they also lack the foundation on how to solve them. This challenge gave birth to a philosophical excavation of molecular and developmental biology. …

3: "The Current Extinction: Defaunation & Ecosystem Disruption", Sarah O'Leary-Driscoll, Crystal Randall

Ecosystem Disruption & Climate Change

Information taken from: Dirzo, R. et al (2014). Defaunation in the Anthropocene. Science, 345(401).

Scientists estimate, conservatively, that there are 5 to 9 million different animal species on the planet. But that number is continually changing, and unfortunately, dropping, as we are likely losing 11,000- 58,000 species annually, and evidence suggests that on average, there has been a decline of about 28% in terms of numbers of individuals within a species over the last four decades. Both of these statistics are vitally important. The critical nature of the first is perhaps more obvious, as total loss of a species is …

Integration Of Biosensors Based On Microfluidic: A Review, A.T. Yousefi, Samira Bagheri, Nahrizul Adib Kadri

Research Publications (2011 to 2015)

Purpose - Biotechnology is closely associated to microfluidics. During the last decade, designs of microfluidic devices such as geometries and scales have been modified and improved according to the applications for better performance. Numerous sensor technologies existing in the industry has potential use for clinical applications. Fabrication techniques of microfluidics initially rooted from the electromechanical systems (EMS) technology. Design/methodology/approach - In this review, we emphasized on the most available manufacture approaches to fabricate microchannels, their applications and the properties which make them unique components in biological studies. Findings - Major fundamental and technological advances demonstrate the enhancing of capabilities and …

Developing A Gene Editing System To Study Haplodiploidy In The Jewel Wasp, Nasonia Vitripennis, Emily A. Muller

Scripps Senior Theses

Hymenopteran insects, which include all ants, bees and wasps, reproduce through a poorly understood form of reproduction known as haplodiploidy. A promising experimental system for understanding this developmental process is the jewel wasp, Nasonia vitripennis. A critical aspect of using Nasonia as a model is establishing an effective means for editing specific genes of interest so that their functions can be studied through genetic means. For my thesis research, I performed a pilot study of the gene editing method known as CRISPR in Nasonia. I targeted the single heterochromatin protein 1 (HP1) gene present in the Nasonia genome …

Chronic Corticosterone Exposure Persistently Elevates The Expression Of Memory-Related Genes In The Lateral Amygdala And Enhances The Consolidation Of A Pavlovian Fear Memory, Melissa S. Monsey, Lara M. Boyle, Melinda L. Zhang, Caroline P. Nguyen, Hope G. Kronman, Kristie T. Ota, Ronald S. Duman, Jane R. Taylor, Glenn E. Schafe

Publications and Research

Chronic exposure to stress has been widely implicated in the development of anxiety disorders, yet relatively little is known about the long-term effects of chronic stress on amygdala-dependent memory formation. Here, we examined the effects of a history of chronic exposure to the stress-associated adrenal steroid corticosterone (CORT) on the consolidation of a fear memory and the expression of memory-related immediate early genes (IEGs) in the lateral nucleus of the amygdala (LA). Rats received chronic exposure to CORT (50 μg/ml) in their drinking water for 2 weeks and were then titrated off the CORT for an additional 6 days followed …

Interview With Jane West - Oh 274, Martha Jane Cato West

Winthrop University Oral History Program

In her July 15, 2013 interview with Martha Manning, Jane Cato West (Class of 1968) details her time at Winthrop as a Biology major in the 1960s. Briefly, West discusses student life and Winthrop traditions, but she provides insight into her life student teaching at Florence High School. Additionally, West includes her experience in required courses like English and Math, as well as experiments conducted in her Biology courses. West concludes the interview with her overall perceptions of Winthrop.

Viability Of Butternut (Juglans Cinerea L) In The Northeastern United States: An Assessment Of The Genetic Diversity, Health, And Hybridization And Recruitment Of Butternut In The Northeast, Andre A W Boraks

Master's Theses and Capstones

Butternut (Juglans cinerea) trees are being extirpated from their natural range by means of an epidemic caused by a fungal pathogen. Widespread mortality is reminiscent of past epidemics on American chestnut (Castanea dentata) and American elm (Ulmus americana ). Understanding the course of contemporary decline of this tree species will provide greater insight on processes of potential extinction and the results of management to prevent it.

This thesis applies an interdisciplinary approach to characterize butternut of the northeastern Unites States. While there is evidence of weak genetic structuring, butternut appears to have maintained sufficient dispersal to prevent isolation and inbreeding …

Characterization Of The Stem Cell Niche In Drosophila Testes, Vittorio Mena Jr.

Seton Hall University Dissertations and Theses (ETDs)

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Genotyping On Custom Arrays Using A Parallel Data Pipeline, Brian Albere

Master's Theses and Capstones

In the past, genotyping (determining a set of alleles in an organism) has been an extremely challenging process. The time, monetary, and technology demands of the task have limited genotype data to a small variety of scientific model organisms with the capacity to conduct genetic crosses. New sequencing technology from companies such as NimbleGen, however, can generate custom organism-specific microarrays at relatively low cost. The combination of these arrays and the knowledge of species' genome-wide SNPs allow genotype experiments, such as generation maps, QTL studies, and natural population variation studies, to be conducted on virtually any organism. Although the NimbleGen …

Distribution Of Mysis Diluviana In Nearshore Lake Ontario, Sean J. Maxwell

Master's Theses and Capstones

Opossum shrimp Mysis diluviana are an important trophic link in Lake Ontario, and in all of the lakes where they occur. Their temporal and spatial distributions are not well documented in the nearshore area (<30 m bottom depth), where alewives and other fish increasingly depend on them as a food source. This study describes their distributions over three years (2009 to 2011) from April through November using data collected from Tucker trawls and multi-frequency hydroacoustics; both deployed over incremental depths. Mysis was found to occur in the nearshore area in 69.6% of the trawl surveys. Maximum densities occurred in April and early May and were located in the deepest (30-m) contour. The density and frequency of occurrence of Mysis increased exponentially from shore. Hydroacoustic methods were effective in quantifying of Mysis density in 42% of the echograms when they were present in simultaneously obtained samples.

Karl E. Peace Papers, Zach S. Henderson Library Special Collections

Finding Aids

This collection consists of the personal and research papers of Karl E. Peace, Professor of Biostatistics at Georgia Southern University and namesake of the Karl E. Peace Center for Biostatistics and Survey Research. Materials span 1941 to 2018 and include, correspondence, teaching materials, published articles, and manuscripts. A small portion of 3 photographs and artists renderings are also included. This collection is still undergoing processing.

Find this collection in the University Libraries' catalog.

Changes In Perceived Self-Efficacy And Attitude Toward Science And Teaching Science In Elementary School, Betsy Ann Sullivan

Dissertations

This study was developed in an effort to ascertain if a proposed biological laboratory curriculum as developed and modeled by the instructor would affect the attitudes and perceived self-efficacy towards science, science teaching and ability to learn science of pre-service elementary teachers. Self-regulated learning (SRL) strategies were incorporated as the variation. Attitudinal topics investigated were the perceived ability to learn science and to teach science.

Students in one biology for non-science majors' biology laboratory class at the University of Southern Mississippi participated in this case study. The group participated in the modified laboratory section which utilized SRL activities, including reflections …

Measurements Of Resistance And Reactance In Fish With The Use Of Bioelectrical Impedance Analysis: Sources Of Error, M. Cox, Ron Heintz, Kyle Hartman

Faculty & Staff Scholarship

New technologies can be riddled with unforeseen sources of error, jeopardizing the validity and application of their advancement. Bioelectrical impedance analysis (BIA) is a new technology in fisheries research that is capable of estimating proximate composition, condition, and energy content in fish quickly, cheaply, and (after calibration) without the need to sacrifice fish. Before BIA can be widely accepted in fisheries science, it is necessary to identify sources of error and determine a means to minimize potential errors with this analysis. We conducted controlled laboratory experiments to identify sources of errors within BIA measurements. We concluded that electrode needle location, …

Gonadotropin Releasing Hormone In Agnathans, Scott Ira Kavanaugh

Doctoral Dissertations

Hagfish and lampreys are the only two representatives of Agnathans among extant vertebrates. The regulatory hypothalamic neurohormone, gonadotropin-releasing hormone (GnRH), regulates reproduction in all vertebrates through the hypothalamic-pituitary-gonadal axis. Most vertebrates have at least two forms of GnRH in the brain. In hagfish the primary amino acid structure of GnRH has not been identified as yet, however, indirect methods have shown an immunoreactive GnRH or a GnRH-like peptide in the brain of hagfish. In addition concentrations of brain immunoreactive-GnRH have been correlated with reproductive stages in the Atlantic hagfish ( Myxine glutinosa). Therefore the objective of the first study was …

Microbial Diversity And Geochemical Energy Sources Of Tutum Bay, Ambitle Island, Papua New Guinea, An Arsenic-Rich, Shallow-Sea Hydrothermal System, Nancy Akerman

All Theses and Dissertations (ETDs)

I investigate the hydrothermal system located in Tutum Bay, Ambitle Island, Papua New Guinea, a shallow-sea system ~5 - 10 meters below sea level that is arsenic-rich. Hydrothermal vents in the bay expel fluids with arsenite: AsIII) concentrations as high as 950 μg/L. To determine the role that Tutum Bay microorganisms might play in mediating As-redox reactions, three approaches were used: analyzing the geochemical environment for energy sources, characterizing the archaeal community composition of the sediments, and conducting culture-dependent As-cycling experiments. The second chapter of this dissertation discusses an energetic study of potential chemolithotrophic metabolic reactions, including As-redox reactions. Results …