Digital Commons Network^™

Fungi On White-Nose Infected Bats (Myotis Spp.) In Eastern Canada Show No Decline In Diversity Associated With Pseudogymnoascus Destructans (Ascomycota: Pseudeurotiaceae), Karen J. Vanderwolf, David Malloch, Donald F. Mcalpine

International Journal of Speleology

The introduction of the fungal pathogen Pseudogymnoascus destructans (Pd) to North America has stimulated research on the poorly known mycology of caves. It is possible that the introduction of Pd reduces the diversity of fungi associated with bats hibernating in caves. To test this hypothesis we examined the fungal assemblages associated with hibernating bats (Myotis spp.) pre- and post- white-nose syndrome (WNS) infection in eastern Canada using culture-dependent methods. We found the mean number of fungal taxa isolated from bats/hibernaculum was not significantly different between pre-infection (29.6 ± 6.1SD) and post-infection with WNS (32.4 ± 4.3). Although …

Mcicsl Newsletter - July 2015, Shannon R. Trimboli Education Coordinator

MCICSL Newsletter

This issue includes the following:

Interns with The Nature Conservancy Participate in Research at Mammoth Cave

Renaissance in Cave Biology Research Underway

as well as Summer research projects, in the news and White-nose Syndrome workshop

Antibodies To Pseudogymnoascus Destructans Are Not Sufficient For Protection Against White-Nose Syndrome, Joseph S. Johnson, Deeann M. Reeder, Thomas M. Lilley

KIP Articles

White-nose syndrome (WNS) is a fungal disease caused by Pseudogymnoascus destructans (Pd) that affects bats during hibernation. Although millions of bats have died from WNS in North America, mass mortality has not been observed among European bats infected by the fungus, leading to the suggestion that bats in Europe are immune. We tested the hypothesis that an antibody-mediated immune response can provide protection against WNS by quantifying antibodies reactive to Pd in blood samples from seven species of free-ranging bats in North America and two free-ranging species in Europe. We also quantified antibodies in blood samples from little brown myotis …

Bats And Disease: Behavioral And Community Responses Of Southern Bat Populations During The White-Nose Syndrome Epizootic, Riley Fehr Bernard

Doctoral Dissertations

This dissertation investigates regional differences in the behavior and activity of bats in eastern North America during the white-nose syndrome epizootic, specifically in the understudied region of the Southeastern United States. An introductory section provides a brief review of the history of white-nose syndrome, an emerging infectious disease in bats, and its introduction into North America. Chapter one provides the first documented evidence of bat activity outside of hibernacula throughout winter. The research presented in chapter two attempts to explain the variation in load and prevalence of P. destructans among species, sites and between years. Finally, chapter three examines the …

The White-Nose Syndrome Transcriptome: Activation Of Anti-Fungal Host Responses In Wing Tissue Of Hibernating Little Brown Myotis, Kenneth A. Field, Joseph Johnson, Thomas M. Lilley

KIP Articles

White-nose syndrome (WNS) in North American bats is caused by an invasive cutaneous infection by the psychrophilic fungus Pseudogymnoascus destructans (Pd). We compared transcriptome-wide changes in gene expression using RNA-Seq on wing skin tissue from hibernating little brown myotis (Myotis lucifugus) with WNS to bats without Pd exposure. We found that WNS caused significant changes in gene expression in hibernating bats including pathways involved in inflammation, wound healing, and metabolism. Local acute inflammatory responses were initiated by fungal invasion. Gene expression was increased for inflammatory cytokines, including interleukins (IL) IL-1β, IL-6, IL-17C, IL-20, IL-23A, IL-24, and G-CSF and chemokines, such …

Trash To Treasure: Assessing Viability Of Wing Biopsies For Use In Bat Genetic Research, Mary Beth Manjerovic, Michelle L. Green, Andrew N. Miller

KIP Articles

The outbreak of white-nose syndrome in North American bats has resulted in massive data collection efforts to characterize the fungus, Pseudogymnoascus destructans. Wing biopsies routinely are collected from live bats, placed in agar media to culture the fungus, and ultimately discarded. We tested whether these discarded tissues represent a viable source of host bat DNA. We found no difference in DNA concentration and no reduction of DNA quality between samples that were extracted immediately compared to samples placed in agar for fungal culture. Although recovered quantities were low, concentrations increased using a cleanup kit. Our study suggests samples collected from …

Range-Wide Genetic Analysis Of Little Brown Bat (Myotis Lucifugus) Populations: Estimating The Risk Of Spread Of White-Nose Syndrome, Maarten J. Vonhof, Amy L. Russell, Cassandra M. Miller-Butterworth

KIP Articles

The little brown bat (Myotis lucifugus) is one of the most widespread bat species in North America and is experiencing severe population declines because of an emerging fungal disease, white-nose syndrome (WNS). To manage and conserve this species effectively it is important to understand patterns of gene flow and population connectivity to identify possible barriers to disease transmission. However, little is known about the population genetic structure of little brown bats, and to date, no studies have investigated population structure across their entire range. We examined mitochondrial DNA and nuclear microsatellites in 637 little brown bats (including all currently recognized …

Post-White-Nose Syndrome Trends In Virginia’S Cave Bats, 2008-2013, Karen E. Powers, Richard J. Reynolds, Wil Orndorff

KIP Articles

Since its 2009 detection in Virginia hibernacula, the fungal pathogen Pseudogymnoascus destructans causing White-nose Syndrome (WNS) has had a marked impact on cave bats locally. From 2008-2013, we documented numeric and physiologic changes in cave bats through fall swarm (FS), early hibernation (EH), and late hibernation (LH) capture and banding surveys at 18 hibernacula in western Virginia. We coupled active surveys with passive biennial winter counts in 2009, 2011, and 2013. We compared individual body mass index (BMI) across years for FS, EH, and LH hibernation to determine if WNS impacts on extant bats would be manifested by changes in …

Impacts Of Climate, Geography And White-Nose Syndrome Mortality On The Population Genetic Structure And Variation Of Myotis Lucifugus In The Eastern United States, Shannon Marie Dodge Morgan

Legacy Theses & Dissertations (2009 - 2024)

A myriad of biotic and abiotic factors contribute to the fluctuations in geographic range and size of animal populations. In the Nearctic, bat species have experienced drastic historic changes in available habitat due to Pleistocene glaciations, and are recently imperiled by the introduction of a novel fungal pathogen, Pseudogymnoascus destructans within the last decade. Myotis lucifugus in particular has lost 90-95% of the population in the eastern US due to the fungal disease White-Nose Syndrome (WNS). A population decline of this magnitude will likely result in a loss of allelic diversity as a result of a genetic bottleneck. My study …