Digital Commons Network^™

The Lineage-Specific Evolution Of Aquaporin Gene Clusters Facilitated Tetrapod Terrestrial Adaptation, Roderick Nigel Finn, François Chauvigné, Jón Baldur Hlidberg, Christopher P. Cutler, Joan Cerdà

Biology: Faculty Publications

A major physiological barrier for aquatic organisms adapting to terrestrial life is dessication in the aerial environment. This barrier was nevertheless overcome by the Devonian ancestors of extant Tetrapoda, but the origin of specific molecular mechanisms that solved this water problem remains largely unknown. Here we show that an ancient aquaporin gene cluster evolved specifically in the sarcopterygian lineage, and subsequently diverged into paralogous forms of AQP2, -5, or -6 to mediate water conservation in extant Tetrapoda. To determine the origin of these apomorphic genomic traits, we combined aquaporin sequencing from jawless and jawed vertebrates with broad taxon assembly of …

Molecular Characterization Of Hemoglobin Protein In Larvae Of 4th Instar Chironomidae For Evaluating Environmental Quality, Jun Taek Oh

Seton Hall University Dissertations and Theses (ETDs)

Studying environmental quality is a challenging task. It is a complicated exercise since the environment is constantly influenced by numerous variables such as climate change, anthropogenic activities, and unexpected natural disasters. Traditionally, exposure to chemical pollutants depended on chemical and physical analysis of environmental media. Unfortunately, this approach has not taken into consideration bioavailability of the chemical(s) of interest to exposed organisms and/or modification of the chemical (bioactivation/detoxification) by the organism. Benthic macroinvertebrates (BMIs) have been chosen as bioindicators for numerous environmental biomonitoring programs geared towards the assessment of aquatic ecosystems. Biomonitoring requires a more subtle measure – a biomarker …

Torch (November 2014), Brandon Baldwin, Civil Rights Team Project

Torch: The Civil Rights Team Project Newsletter

No abstract provided.

Mainecare Health Homes Enrollment In The First Year Of Implementation, Kimberley S. Fox Mpa, Carolyn E. Gray Mph, Katherine Rosingana

Population Health & Health Policy

The MaineCare Health Homes Initiative is designed to improve care coordination for MaineCare members with complex chronic medical conditions. The program is being implemented in two stages: Health Homes (HH), which began in January 2013, focuses on members with chronic health conditions. Behavioral Health Homes (BHH), which began in April 2014, is designed to meet the needs of people with Severe Mental Illness (SMI) or Serious Emotional Disturbances (SED).

The HH first stage builds off the State’s existing multi-payer Patient Centered Medical Home (PCMH) Pilot project and Medicare Advanced Primary Care Practice (MAPCP) Demonstration by providing addon payments to qualifying …

Application Of A Bioenergetics Framework For Assessing Sub-Lethal Effects Of Pollutants In The Freshwater Mussel Elliptio Complanata, Christopher G. Goodchild

All Theses And Dissertations

Although biomarkers are frequently used to assess sublethal effects of contaminants, a lack of mechanistic linkages to higher-level effects limits the predictive power of biomarkers. Bioenergetics has been proposed as a framework for linking cellular effects to whole-animal effects. We investigated sublethal effects of exposure to wastewater treatment facility effluent in freshwater mussels in situ, thereby capturing ecologically relevant exposure conditions. Our study focused on the energetic biomarker AMP-activated protein kinase (AMPK), while also considering more traditional biomarkers like heat shock proteins (HSP70), and antioxidant enzymes (i.e., superoxide dismutase (SOD), glutathione-S-transferase (GST)). We examined biomarkers at mRNA and protein levels. …

Family Affairs Newsletter 2014-07-15, Zack Paakkonen

Family Affairs newsletter (2004-2016)

FAMILY AFFAIRS was a free, twice-a-month, social activities newsletter for the GLBTQI (gay/lesbian/bisexual/trans/queer/intersex) community, sent out around the 1st and 15th of each month. It covered the State of Maine only. The list was begun and maintained for many years by Jean Vermette in Bangor, and later operated by Zack Paakkonen of Portland. Over the years it evolved from a social activities newsletter into a business directory, classified ad service, and community bulletin board.

Alumni At Large

Colby Magazine

Catching Up: Alumni Profiles
More Than Bears: Linda Wrigley '89 M.D.
Anything for Tampa: Kevin Plummer ’89
Pedaling Her Crops: Hannah Converse ’09

Updates

Newsmakers

Obituaries

Material Potential | Recontextualizing Material Libraries, Diana Wagner

Masters Theses

Material libraries are growing resources that offer an opportunity for artists and designers to discover a variety of materials. These collections are composed in a traditional taxonomy, pairing materials based on composition. Samples are typically commercially made and are displayed independent of context, means of production, cultural significance, and application.

Through making we learn a material’s potential: the transformation it can make, the form it can take, and the new composites we can create. This generative experience lives in the studio and workshop. But how can we introduce this language of process, critical thinking, and making to activate and challenge …

Shellfish Closure Trends Near Mt. Desert Island, Maine, Marianne Ferguson

CLAS: Colby Liberal Arts Symposium

Shellfish are a popular commercial fishery harvest in Maine. However, they are susceptible to biotoxins transmitted by toxic phytoplankton, which can lead to paralytic or amnesic shellfish poisoning of the humans that consume them. The Maine Department of Marine Resources has a phytoplankton monitoring program in place to detect such biotoxins and to close areas of shellfish harvesting if need be. Since 2001, they have archived the data from every closure. One of Maines shellfish species, the Blue Mussel, is sensitive to environmental changes and is known as a bio-monitor of pollution. Therefore, a method will be developed to analyze …

Mainecare Stage A Health Homes Year 1 Report: Implementation Findings And Baseline Analysis, Kimberley S. Fox Mpa, Carolyn E. Gray Mph, Katherine Rosingana, Deborah A. Thayer Mba

Population Health & Health Policy

In January 2013, Maine established Health Homes under federal authority pursuant to Section 2703 of the Affordable Care Act to improve care coordination for MaineCare members with chronic conditions. Stage A of the Health Homes initiative focuses on members with complex medical chronic conditions. Stage B, planned for early 2014, will focus on persons with severe and persistent mental health conditions and children with serious emotional disturbances. The Stage A demonstration builds off the State’s existing Maine multi-payer Patient Centered Medical Home (PCMH) Pilot project and Maine’s Medicare Advanced Primary Care Practice (MAPCP) Demonstration by providing add-on payments to primary …

Hassett Coauthors Paper Assessing Global Warming Impact On Tiny Atlantic Crustaceans, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Mechanistic Target Of Rapamycin (Mtor) Signaling Genes In Decapod Crustaceans: Cloning And Tissue Expression Of Mtor, Akt, Rheb, And S6 Kinase In The Green Crab, Carcinus Maenas, And Blackback Land Crab, Gecarcinus Lateralis, Ali M. Abuhagr, Kyle S. Maclea, Ernest S. Chang, Donald L. Mykles

Life Sciences Faculty Scholarship

Mechanistic target of rapamycin (mTOR) controls global translation of mRNA into protein by phosphorylating p70 S6 kinase (S6K) and eIF4E-binding protein-1. Akt and Rheb, a GTP-binding protein, regulate mTOR protein kinase activity. Molting in crustaceans is regulated by ecdysteroids synthesized by a pair of molting glands, or Y-organs (YOs), located in the cephalothorax. During premolt, the YOs hypertrophy and increase production of ecdysteroids. Rapamycin (1 μM) inhibited ecdysteroid secretion in Carcinus maenas and Gecarcinus lateralis YOs in vitro, indicating that ecdysteroidogenesis requires mTOR-dependent protein synthesis. The effects of molting on the expression of four key mTOR signaling genes (mTOR, Akt, …

A Unique Covalent Bond In Basement Membrane Is A Primordial Innovation For Tissue Evolution, Aaron L. Fidler, Roberto M. Vanacore, Sergei V. Chetyrkin, Vadim K. Pedchenko, Gautam Bhave, Viravuth P. Yin, Cody L. Stothers, Kristie Lindsey Rose, W. Hayes Mcdonald, Travis A. Clark, Dorin-Bogdan Borza, Robert E. Steele, Michael T. Ivy, The Aspirnauts, Julie K. Hudson, Billy G. Hudson

Biology Faculty Research

We recently discovered that the collagen IV scaffolds of bovine, mouse, and Drosophila melanogaster tissues are stabilized by sulfilimine chemical bonds (S = N) (20⇓–22). The bond is a covalent cross-link between methionine-93 (Met93) and lysine-211 or hydroxylysine-211 (Lys211/Hyl211) residues that stabilizes the noncollagenous (NC1) interface of adjoining triple helical protomers (Fig. 1A). Peroxidasin, an animal heme peroxidase embedded in basement membrane, forms these sulfilimine cross-links by producing hypohalous acids as intermediates for Met93 oxidization (21, 22). Here, we explored whether the sulfilimine cross-link of collagen IV is a fundamental requirement in the genesis and evolution of tissues in Eumetazoa. …

Facilitating Lewin's Change Model With Collaborative Evaluation In Promoting Evidence Based Practices Of Health Professionals, Julianne Manchester, Deanna L. Gray-Miceli, Judith A. Metcalf, Charlotte A. Paolini, Anne H. Napier, Constance L. Coogle, Myra G. Owens

Virginia Center on Aging Publications

Evidence based practices (EBPs) in clinical settings interact with and adapt to host organizational characteristics. The contextual factors themselves, surrounding health professions’ practices, also adapt as practices become sustained. The authors assert the need for better planning models toward these contextual factors, the influence of which undergird a well-documented science to practice gap in literature on EBPs. The mechanism for EBP planners to anticipate contextual effects as programs Unfreeze their host settings, create Movement, and become Refrozen (Lewin, 1951) is present in Lewin's 3-step change model. Planning for contextual change appears equally important as planning for the actual practice outcomes …

Mutations In The Gene That Encodes The F-Actin Binding Protein Anillin Cause Fsgs, R. A. Gbadegesin, G. Hall, A. Adeyemo, N. Hanke, I. Tossidou, J. Burchette, G. H. Wu, A. Homstad, P. C. Singhal, M. P. Winn, +17 Additional Authors

Journal Articles

FSGS is characterized by segmental scarring of the glomerulus and is a leading cause of kidney failure. Identification of genes causing FSGS has improved our understanding of disease mechanisms and points to defects in the glomerular epithelial cell, the podocyte, as a major factor in disease pathogenesis. Using a combination of genome-wide linkage studies and whole-exonne sequencing in a kindred with familial FSGS, we identified a missense mutation R431C in anillin (ANLN), an F-actin binding cell cycle gene, as a cause of FSGS. We screened 250 additional families with FSGS and found another variant, G618C, that segregates with disease in …

Early Lessons Learned In Implementing Mainecare Health Homes, Kimberley S. Fox Mpa, Carolyn E. Gray Mph, Katherine Rosingana

Population Health & Health Policy

The MaineCare Health Homes Initiative is designed to improve care coordination for MaineCare members with complex chronic medical conditions. The initiative, which began in January 2013, builds off the State’s existing multi-payer Patient Centered Medical Home (PCMH) Pilot project and Medicare Advanced Primary Care Practice (MAPCP) Demonstration by providing add-on payments to primary care practices to provide “whole person” integrated care coordination for MaineCare members with specific chronic conditions. For the highest need patients, practices work with Community Care Teams (CCT) that provide short-term care management and social support services. The Muskie School of Public Service is evaluating the implementation …

Colby College Catalogue 2014 - 2015, Colby College

Colby Catalogues

Colby College Catalogue: September 2014 - August 2015

Natural Selection Canalizes Expression Variation Of Environmentally Induced Plasticity-Enabling Genes, Joseph R. Shaw, Thomas H. Hampton, Benjamin L. King, Andrew Whitehead, Fernando Galvez, Robert H. Gross, Nathan Keith, Emily Notch, Dawoon Jung, Stephen P. Glaholt, Celia Y. Chen, John K. Colbourne, Bruce A. Stanton

Faculty Publications

© 2014 The Author. Many organisms survive fluctuating and extreme environmental conditions by manifesting multiple distinct phenotypes during adulthood by means of developmental processes that enable phenotypic plasticity. We report on the discovery of putative plasticity-enabling genes that are involved in transforming the gill of the euryhaline teleost fish, Fundulus heteroclitus, from its freshwater to its seawater gill-type, a process that alters both morphology and function. Gene expression that normally enables osmotic plasticity is inhibited by arsenic. Gene sets defined by antagonistic interactions between arsenic and salinity show reduced transcriptional variation among individual fish, suggesting unusually accurate and precise regulatory …

Lichens Of Six Vernal Pools In Acadia National Park, Me, Usa, J. Barton, B. Ciccotelli, J. E. Gall, F. C. Olday, B. Connery, T. B. Harris, A. M. Fryday, Nishanta Rajakaruna

Faculty Publications, Biological Sciences

Whereas lichen-habitat relations have been well-documented globally, literature on lichens of vernal pools is scant. We surveyed six vernal pools at Acadia National Park on Mount Desert Island, Maine, USA for their lichen diversity. Sixty-seven species were identified, including seven species that are new reports for Acadia National Park: Fuscidea arboricola, Hypogymnia incurvoides, Lepraria finkii, Phaeographis inusta, Ropalospora viridis, Usnea flammea, and Violella fucata. Five species are considered uncommon or only locally common in New England: Everniastrum catawbiense, Hypogymnia krogiae, Pseudevernia cladonia, Usnea flammea, and Usnea merrillii. This work represents the first survey of lichens from vernal pools in Acadia …

Cellular Coping Mechanisms To Hypoxia In The Longhorn Sculpin (Myoxocephalus Octodecemspinosus) Brain, Kelly Heino

College of Graduate Studies: Theses & Dissertations

The loss of neurological function due to hypoxia remains to be a challenge in many species due to the lack of knowledge and understanding of cellular responses. To investigate cellular responses and survival strategies of the brain during hypoxia and post-hypoxia recovery in a moderate hypoxia-tolerant species, with the hopes of identifying possible therapeutic remedies, thirty Longhorn sculpin, Myoxocephalus octodecimspinosus, were challenged with acute hypoxia (40% O2 saturation for 1 h) and twelve additional sculpin were used as controls (~90% O₂ saturation). Fish were sampled at each of the following time points: control/normoxic conditions (T=0 h); hypoxic conditions …