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2013

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The Processing Of Amyloid Precursor Protein In The Central Nervous System Of Humans And Rhesus Macaques, Justyna Anna Dobrowolska Dec 2013

The Processing Of Amyloid Precursor Protein In The Central Nervous System Of Humans And Rhesus Macaques, Justyna Anna Dobrowolska

All Theses and Dissertations (ETDs)

Alzheimer's disease: AD) is the most common dementia, currently afflicting 30 million people worldwide, with prevalence steadily increasing. Over 99% of AD cases are classified as sporadic, in which the major risk factors are age: greater than 65 years) and the ApoE-ε4 allele: in a gene dose dependent manner). The minority of AD cases: less than 1%) are caused by autosomal dominant inheritance of a genetic mutation in one of three genes: Amyloid Precursor Protein: APP), Presenilin-1: PSEN1), or Presenilin-2: PSEN2). Mutation carriers will generally notice cognitive decline starting at a relatively young age, anytime between their 30s-50s, and eventually …

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Dna Barcoding Of Coastal Larval Fish Communities Of Dongshar Island, South China Sea Revealed By Mitochondrial Co I Sequences, I-Shiung Chen, Kwang-Tsao Shao, Shao-Liang Hsu, Gwo-Ching Gong, Yu-Chih Chen, Ta-Kang Liu Dec 2013

Dna Barcoding Of Coastal Larval Fish Communities Of Dongshar Island, South China Sea Revealed By Mitochondrial Co I Sequences, I-Shiung Chen, Kwang-Tsao Shao, Shao-Liang Hsu, Gwo-Ching Gong, Yu-Chih Chen, Ta-Kang Liu

Journal of Marine Science and Technology–Taiwan

The 70 different samples of larval fish morphotypes have been successfully amplified and sequenced in detail via PCR amplification for the partial sequences of mtDNA CO I gene. The overall genetic similarities ranged from 83% to 100%. Totally 26 different fish species can be detected and analyzed. There are 16 fish species can be identified into specific level. However, there are remaining 12 species can be expected just into suggested fish generic level. Apparently, two major groups of taxa would be the members of Gobiidae and Apogonidae which comprised 50% of total species diversity, and also comprised 67% of total …

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Differentiation Of Fungal Phytopathogens By Ft-Ir And Maldi-Tof Ms, Curtis Muldrow Atkinson Dec 2013

Differentiation Of Fungal Phytopathogens By Ft-Ir And Maldi-Tof Ms, Curtis Muldrow Atkinson

Theses and Dissertations

The use of matrix assisted laser desorption ionization time-oflight mass spectrometry, Fourier transform infrared spectroscopy, and other analytical means of identifying and differentiating microorganisms hold much promise. These analytical tools have been extensively assessed for their ability to differentiate bacteria and fungi. Most of this research has been coordinated in medically relevant microorganisms, but the technology can work just as well with agriculturally important microorganisms. In this thesis, these technologies were reviewed and then subsequently studied for their ability to differentiate Aspergillus species (that devastate corn and other crops yearly with aflatoxin contamination), as well as Macrophomina phaseolina and Thielaviopsis …

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Analysis Of Humeral And Femoral Cross-Sectional Properties At Morton Shell Mound (16ib3), Sarah Marie Zaleski Dec 2013

Analysis Of Humeral And Femoral Cross-Sectional Properties At Morton Shell Mound (16ib3), Sarah Marie Zaleski

Theses and Dissertations

Using the concept of bone functional adaptation, this study analyzes femoral and humeral cross-sectional properties of human skeletal materials from Morton Shell Mound on the Louisiana coast. This work helps fill a gap in such analyses in the southern U.S. and contributes to an understanding of the functional adaptation of the human skeleton. Properties were compared to those of other prehistoric Southeastern fisher-hunter-gatherers from Gold Mine, Plash Island, and several Georgia coast sites to assess mobility and activity patterns among inland and coastal groups. Less sexual dimorphism of femoral midshaft shape among coastal Morton and Plash, compared to inland Gold …

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How Does Student Understanding Of A Concept Change Throughout A Unit Of Instruction? Support Toward The Theory Of Learning Progressions, Brian Jay Dyer Dec 2013

How Does Student Understanding Of A Concept Change Throughout A Unit Of Instruction? Support Toward The Theory Of Learning Progressions, Brian Jay Dyer

Dissertations and Theses

This study documented the changes in understanding a class of eighth grade high school-level biology students experienced through a biology unit introducing genetics. Learning profiles for 55 students were created using concept maps and interviews as qualitative and quantitative instruments. The study provides additional support to the theory of learning progressions called for by experts in the field. The students' learning profiles were assessed to determine the alignment with a researcher-developed learning profile. The researcher-developed learning profile incorporated the learning progressions published in the Next Generation Science Standards, as well as current research in learning progressions for 5-10th grade students …

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Mathematical Model Of Dynamic Protein Interactions Regulating P53 Protein Stability For Tumor Suppression, Hua Wang, Guang Peng Dec 2013

Mathematical Model Of Dynamic Protein Interactions Regulating P53 Protein Stability For Tumor Suppression, Hua Wang, Guang Peng

Mathematical Sciences: Faculty Publications

In the field of cancer biology, numerous genes or proteins form extremely complex regulatory network, which determines cancer cell fate and cancer cell survival. p53 is a major tumor suppressor that is lost in more than 50% of human cancers. It has been well known that a variety of proteins regulate its protein stability, which is essential for its tumor suppressive function. It remains elusive how we could understand and target p53 stabilization process through network analysis. In this paper we discuss the use of random walk and stationary distribution to measure the compound effect of a network of genes …

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Towards Detection And Control Of Civilian Unmanned Aerial Vehicles, Matthew Peacock, Michael N. Johnstone Dec 2013

Towards Detection And Control Of Civilian Unmanned Aerial Vehicles, Matthew Peacock, Michael N. Johnstone

Australian Information Warfare and Security Conference

Considering the significant number of non‐military unmanned aerial vehicles (UAVs) that can be purchased to operate in unregulated air space and the range of such devices, the potential for security and privacy problems to arise is significant. This can lead to consequent harm for critical infrastructure in the event of these UAVs being used for criminal or terrorist purposes. Further, if these devices are not being detected, there is a privacy problem to be addressed as well. In this paper we test a specific UAV, the Parrot AR Drone version 2, and present a forensic analysis of tests used to …

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Bismuth Nanoparticles As Medical X-Ray Contrast Agents: Synthesis, Characterization And Applications, Anna Laura Brown Dec 2013

Bismuth Nanoparticles As Medical X-Ray Contrast Agents: Synthesis, Characterization And Applications, Anna Laura Brown

Dissertations and Theses

Bismuth based nanomaterials have recently attracted attention as heavy element X-ray contrast agents because of the high atomic number and predicted biological compatibility of bismuth. Nanoparticle X-ray contrast agents may enable a number of novel medical imaging applications, including blood pool and site-directed imaging. However these hypothetical applications are hindered by lack of suitable synthetic methods for production of imaging agents. This dissertation describes synthesis of a novel class of bismuth nanoparticles that are aqueously stabilized using poly and monosaccharides. These particles are synthesized using highly biologically compatible reagents and are oxidatively stable in water and in moderately basic buffered …

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Layered Long Term Co-Culture Of Hepatocytes And Endothelial Cells On A Transwell Membrane: Toward Engineering The Liver Sinusoid, Young Bok (Abraham) Kang, Siddhartha Rawat, Joseph Cirillo, Michael Bouchard, Hongseok (Moses) Noh Dec 2013

Layered Long Term Co-Culture Of Hepatocytes And Endothelial Cells On A Transwell Membrane: Toward Engineering The Liver Sinusoid, Young Bok (Abraham) Kang, Siddhartha Rawat, Joseph Cirillo, Michael Bouchard, Hongseok (Moses) Noh

Faculty Publications - Biomedical, Mechanical, and Civil Engineering

This paper presents a novel liver model that mimics the liver sinusoid where most liver activities occur. A key aspect of our current liver model is a layered co-culture of primary rat hepatocytes (PRHs) and primary rat liver sinusoidal endothelial cells (LSECs) or bovine aortic endothelial cells (BAECs) on a transwell membrane. When a layered co-culture was attempted with a thin matrigel layer placed between hepatocytes and endothelial cells to mimic the Space of Disse, the cells did not form completely separated monolayers. However, when hepatocytes and endothelial cells were cultured on the opposite sides of a transwell membrane, PRHs …

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Genetic Variability Of Spined Soldier Bugs (Hemiptera: Pentatomidae) Sampled From Distinct Field Sites And Laboratory Colonies In The United States, Fatima Mustafa, Muhammad Ullah, Kathleen M. Kneeland, Thomas A. Coudron, W. Wyatt Hoback, S. R. Skoda, Jaime Molina-Ochoa, John E. Foster Dec 2013

Genetic Variability Of Spined Soldier Bugs (Hemiptera: Pentatomidae) Sampled From Distinct Field Sites And Laboratory Colonies In The United States, Fatima Mustafa, Muhammad Ullah, Kathleen M. Kneeland, Thomas A. Coudron, W. Wyatt Hoback, S. R. Skoda, Jaime Molina-Ochoa, John E. Foster

Department of Entomology: Faculty Publications

The spined soldier bug, Podisus maculiventris (Say), is an important biological control agent of agricultural and forest pests; and it preys on eggs and larvae of lepidopteran and coleopteran species. Genetic variability among field samples collected from Michigan, Mississippi, Missouri, Florida and established laboratory colonies was examined using Amplified Fragment Length Polymorphism-Polymerase Chain Reaction (AFLP-PCR). Four AFLP primer pairs generated a total of 340 molecular markers for evaluation. Results from Analysis of Molecular Variance showed that the majority of the genetic variation occurred within populations (individuals from each sample site). Nei’s method indicated reduced genetic diversity in laboratory populations compared …

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The Log-Beta Generalized Half-Normal Regression Model, Rodrigo R. Pescim, Edwin M. M. Ortega, Gauss M. Cordeiro, Clarice G. B. Demtrio, Gholamhossein Hamedani Dec 2013

The Log-Beta Generalized Half-Normal Regression Model, Rodrigo R. Pescim, Edwin M. M. Ortega, Gauss M. Cordeiro, Clarice G. B. Demtrio, Gholamhossein Hamedani

Mathematics, Statistics and Computer Science Faculty Research and Publications

We introduce a log-linear regression model based on the beta generalized half-normal distribution (Pescim et al., 2010). We formulate and develop a log-linear model using a new distribution so-called the log-beta generalized half normal distribution. We derive expansions for the cumulative distribution and density functions which do not depend on complicated functions. We obtain formal expressions for the moments and moment generating function. We characterize the proposed distribution using a simple relationship between two truncated moments. An advantage of the new distribution is that it includes as special sub-models classical distributions reported in the lifetime literature. We also show that …

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Synthesis Of Resveratrol Ester Derivatives, Daniel Ressler Dec 2013

Synthesis Of Resveratrol Ester Derivatives, Daniel Ressler

Electronic Theses and Dissertations

The goal of this research project was to synthesize derivatives of transresveratrol. In order for resveratrol to be activated and used by the body it needs to bind to Human Serum Albumin (HSA), a protein in blood plasma. The derivatives were synthesized to improve the ability of resveratrol to enter cells as well as improve their ability to bind to HSA. The three derivatives that were synthesized have converted one of the hydroxyl groups on resveratrol to an ether with a methylene chain terminated by a carboxylic acid. By varying the lengths of the methylene chain we varied the water …

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The Design, Synthesis And Biological Evaluation Of Novel Taxoid Anticancer Agents And Their Tumor-Targeted Drug Conjugates, Joshua Seitz Dec 2013

The Design, Synthesis And Biological Evaluation Of Novel Taxoid Anticancer Agents And Their Tumor-Targeted Drug Conjugates, Joshua Seitz

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Cancer is the second leading cause of death in the United States and is responsible for one in eight deaths worldwide. Traditional chemotherapeutics target the processes involved in cell division and consequently produce dangerous and dose-limiting side effects. In an effort to identify the therapeutic windown of these drugs, tumor targeted drug conjugates (TTDCs) are currently under development. The Ojima lab has developed and extensive panel of next-generation taxoids that possess the requisite sub-nanomolar potency for incorporation into effective conjugates. A small library of 17 taxoids was synthesized and their cytotoxicity investigated. Selected taxoids were synthesized on the half-gram to …

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Why Have The Peninsular "Negritos" Remained Distinct?, Geoffrey Benjamin Nov 2013

Why Have The Peninsular "Negritos" Remained Distinct?, Geoffrey Benjamin

Human Biology

The primary focus of this article is on the so-called negritos of Peninsular Malaysia and southern Thailand, but attention is also paid to other parts of Southeast Asia. I present a survey of current views on the "negrito" phenotype—is it single or many? If the phenotype is many (as now seems likely), it must have resulted from parallel evolution in the several different regions where it has been claimed to exist. This would suggest (contrary to certain views that have been expressed on the basis of very partial genetic data) that the phenotype originated recently and by biologically well-authenticated processes …

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Design Of Engineered Biomaterial Architectures Through Natural Silk Proteins, Nicholas Kurland Nov 2013

Design Of Engineered Biomaterial Architectures Through Natural Silk Proteins, Nicholas Kurland

Theses and Dissertations

Silk proteins have provided a source of unique and versatile building blocks in the fabrication of biomedical devices for addressing a range of applications. Critical to advancing this field is the ability to establish an understanding of these proteins in their native and engineered states as well as in developing scalable processing strategies, which can fully exploit or enhance the stability, structure, and functionality of the two constituent proteins, silk fibroin and sericin. The research outlined in this dissertation focuses on the evolution in architecture and capability of silks, to effectively position a functionally-diverse, renewable class of silk materials within …

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Cell-Based Multi-Parametric Model Of Cleft Progression During Submandibular Salivary Gland Branching Morphogenesis, Shayoni Ray, Daniel Yuan, Nimit Dhulekar, Basak Oztan, Bu¨Lent Bu¨Lent Yener, Melinda Larsen Nov 2013

Cell-Based Multi-Parametric Model Of Cleft Progression During Submandibular Salivary Gland Branching Morphogenesis, Shayoni Ray, Daniel Yuan, Nimit Dhulekar, Basak Oztan, Bu¨Lent Bu¨Lent Yener, Melinda Larsen

Biological Sciences Faculty Scholarship

Cleft formation during submandibular salivary gland branching morphogenesis is the critical step initiating the growth and development of the complex adult organ. Previous experimental studies indicated requirements for several epithelial cellular processes, such as proliferation, migration, cell-cell adhesion, cell-extracellular matrix (matrix) adhesion, and cellular contraction in cleft formation; however, the relative contribution of each of these processes is not fully understood since it is not possible to experimentally manipulate each factor independently. We present here a comprehensive analysis of several cellular parameters regulating cleft progression during branching morphogenesis in the epithelial tissue of an early embryonic salivary gland at a …

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“The Flow Of Blood In Nature” Franz Marc’S Animal Theory, Morgan Rinehart Nov 2013

“The Flow Of Blood In Nature” Franz Marc’S Animal Theory, Morgan Rinehart

Theses and Dissertations

This thesis argues for a coherent theory of the animal in the written and visual works of the German Expressionist painter, Franz Marc. By contextualizing Marc’s animal theory within the history of animal studies, this thesis will analyze how Marc’s animal theory corresponds with several central concepts within this field. One of these concepts—a theory of animal death—is central to the artist’s greater theory of the animal and to the analysis this thesis provides. In examining Marc’s theory of animal death, the following work will propose that the artist’s theory of animal spirituality is his greatest legacy within the field …

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Bdnf In The Dentate Gyrus Is Required For Consolidation Of "Pattern-Separated" Memories., Pedro Bekinschtein, Brianne A Kent, Charlotte A Oomen, Gregory D Clemenson, Fred H Gage, Lisa M Saksida, Timothy J Bussey Nov 2013

Bdnf In The Dentate Gyrus Is Required For Consolidation Of "Pattern-Separated" Memories., Pedro Bekinschtein, Brianne A Kent, Charlotte A Oomen, Gregory D Clemenson, Fred H Gage, Lisa M Saksida, Timothy J Bussey

Brain and Mind Institute Researchers' Publications

Successful memory involves not only remembering information over time, but also keeping memories distinct and less confusable. The computational process for making representations for similar input patterns more distinct from each other has been referred to as "pattern separation." In this work, we developed a set of behavioral conditions that allowed us to manipulate the load for pattern separation at different stages of memory. Thus, we provide experimental evidence that a brain-derived neurotrophic factor (BDNF)-dependent pattern separation process occurs during the encoding/storage/consolidation, but not the retrieval stage of memory processing. We also found that a spontaneous increase in BDNF in …

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Evidence For Finely-Regulated Asynchronous Growth Of Toxoplasma Gondii Cysts Based On Data-Driven Model Selection, Adam M. Sullivan, Xiaopeng Zhao, Yasuhiro Suzuki, Eri Ochiai, Stephen Crutcher, Michael A. Gilchrist Nov 2013

Evidence For Finely-Regulated Asynchronous Growth Of Toxoplasma Gondii Cysts Based On Data-Driven Model Selection, Adam M. Sullivan, Xiaopeng Zhao, Yasuhiro Suzuki, Eri Ochiai, Stephen Crutcher, Michael A. Gilchrist

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Toxoplasma gondii establishes a chronic infection by forming cysts preferentially in the brain. This chronic infection is one of the most common parasitic infections in humans and can be reactivated to develop life-threatening toxoplasmic encephalitis in immunocompromised patients. Host-pathogen interactions during the chronic infection include growth of the cysts and their removal by both natural rupture and elimination by the immune system. Analyzing these interactions is important for understanding the pathogenesis of this common infection. We developed a differential equation framework of cyst growth and employed Akaike Information Criteria (AIC) to determine the growth and removal functions that best describe …

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Alginate Hydrogel As A Three-Dimensional Extracellular Matrix For In Vitro Models Of Development, Catherine Sargus-Patino Nov 2013

Alginate Hydrogel As A Three-Dimensional Extracellular Matrix For In Vitro Models Of Development, Catherine Sargus-Patino

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Tissue engineering, involving the use of three-dimensional (3-D) scaffolds for cell and tissue culture, is a promising technique for establishing in vitro culture models that mimic in vivo environments. In vitro models present ethical and cost advantages over in vivo models, and allow for controlled, mechanistic studies on factors that regulate normal and abnormal tissue development. Alginate, a linear polysaccharide derived from brown algae, has properties that make it a favorable material as a 3-D extracellular matrix for in vitro cell and tissue models. After demonstrating alginate’s tunable physical and chemical properties with respect to gel formation, stiffness, and cellular …

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Dna Barcoding Distinguishes Pest Species Of The Black Fly Genus Cnephia (Diptera: Simuliidae), I. M. Confitti, K. P. Pruess, A. Cywinska, T. O. Powers, D. C. Currie Nov 2013

Dna Barcoding Distinguishes Pest Species Of The Black Fly Genus Cnephia (Diptera: Simuliidae), I. M. Confitti, K. P. Pruess, A. Cywinska, T. O. Powers, D. C. Currie

Department of Entomology: Faculty Publications

Accurate species identification is essential for cost-effective pest control strategies. We tested the utility of COI barcodes for identifying members of the black fly genus Cnephia Enderlein (Diptera: Simuliidae). Our efforts focus on four Nearctic Cnephia species—Cnephia dacotensis (Dyar & Shannon), Cnephia eremities Shewell, Cnephia ornithophilia (Davies, Peterson & Wood), and Cnephia pecuarum (Riley)—the latter two being current or potential targets of biological control programs. We also analyzed one Palearctic species, Cnephia pallipes (Fries). Although Cnephia adults can be identified anatomically to species, control programs target the larval stage, which is difficult or impossible to distinguish morphologically. By using …

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Disruption Of Drosophila Melanogaster Lipid Metabolism Genes Causes Tissue Overgrowth Associated With Altered Developmental Signaling., Takeshi Sasamura, Kenji Matsuno, Mark E Fortini Nov 2013

Disruption Of Drosophila Melanogaster Lipid Metabolism Genes Causes Tissue Overgrowth Associated With Altered Developmental Signaling., Takeshi Sasamura, Kenji Matsuno, Mark E Fortini

Department of Biochemistry and Molecular Biology Faculty Papers

Developmental patterning requires the precise interplay of numerous intercellular signaling pathways to ensure that cells are properly specified during tissue formation and organogenesis. The spatiotemporal function of many developmental pathways is strongly influenced by the biosynthesis and intracellular trafficking of signaling components. Receptors and ligands must be trafficked to the cell surface where they interact, and their subsequent endocytic internalization and endosomal trafficking is critical for both signal propagation and its down-modulation. In a forward genetic screen for mutations that alter intracellular Notch receptor trafficking in Drosophila melanogaster, we recovered mutants that disrupt genes encoding serine palmitoyltransferase and acetyl-CoA carboxylase. …

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Annual Report: 2012, N/A N/A Oct 2013

Annual Report: 2012, N/A N/A

Annual Reports

I submit herewith the annual reports from the Agricultural and Forestry Experiment Station, School of Natural Resources and Agricultural Sciences, University of Alaska Fairbanks, for the period ending December 31, 2012. This is done in accordance with an act of Congress, approved March 2, 1887, entitled, “An act to establish agricultural experiment stations, in connection with the agricultural college established in the several states under the provisions of an act approved July 2, 1862, and under the acts supplementary thereto,” and also of the act of the Alaska Territorial Legislature, approved March 12, 1935, accepting the provisions of the act …

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Multi-Factorial Stability Study Of Metabolites, Ruijun Zheng, John A. Morgan Oct 2013

Multi-Factorial Stability Study Of Metabolites, Ruijun Zheng, John A. Morgan

The Summer Undergraduate Research Fellowship (SURF) Symposium

Previous research done on metabolites has found that high energy compounds like adenosine triphosphate(ATP), nicotinamide adenine dinucleotide phosphate reduced (NADPH) or nicotinamide adenine dinucleotide reduced (NADH) are unstable in cell extracts at relatively high temperature and acidic condition. The multifactorial stability study of metabolites enables us to verify the best condition of storage for these energy compounds and sugar phosphates, as well as to strengthen the understanding of metabolism in biological study. As the most solvents for high performance liquid chromatography (HPLC) are acidic and unfavorable for these energy compounds, it is necessary to perform a detailed study about …

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Synthesis And Characterization Of Novel Amino-Retinoid A4-Spermine, Jeffrey Swallow, Dr. Heidi Vollmer-Snarr Oct 2013

Synthesis And Characterization Of Novel Amino-Retinoid A4-Spermine, Jeffrey Swallow, Dr. Heidi Vollmer-Snarr

Journal of Undergraduate Research

Age Related Macular Degeneration (AMD) is the leading cause of vision loss in developed countries. The accumulation of lipofuscin in the eye causes death of the retinal pigment epithelium (RPE) leading to blindness (1,2). The lipofuscin chemical A2E has been shown to have cytotoxic effect in RPE, yet much of lipofuscin has remained uncharacterized (3). We have shown that other compounds exist in lipofuscin through preliminary mass spectroscopy (MS) and high-pressure liquid chromatography (HPLC) data collected from lipofuscin samples. Our lab is working to first characterize these lipofuscin compounds and then we will also study their oxidative products in order …

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Function And Distribution Of Apolipoprotein A1 In The Artery Wall Are Markedly Distinct From Those In Plasma, Joseph A. Didonato, Ying Huang, Kulwant S. Aulak, Orli Even-Or, Gary Gerstenecker, Valentin Gogonea, Yuping Wu, Paul L. Fox, W.H. Wilson Tang, Edward F. Plow, Jonathan D. Smith, Edward A. Fisher, Stanley L. Hazen Oct 2013

Function And Distribution Of Apolipoprotein A1 In The Artery Wall Are Markedly Distinct From Those In Plasma, Joseph A. Didonato, Ying Huang, Kulwant S. Aulak, Orli Even-Or, Gary Gerstenecker, Valentin Gogonea, Yuping Wu, Paul L. Fox, W.H. Wilson Tang, Edward F. Plow, Jonathan D. Smith, Edward A. Fisher, Stanley L. Hazen

Chemistry Faculty Publications

Background—Prior studies show that apolipoprotein A1 (apoA1) recovered from human atherosclerotic lesions is highly oxidized. Ex vivo oxidation of apoA1 or high-density lipoprotein (HDL) cross-links apoA1 and impairs lipid binding, cholesterol efflux, and lecithin-cholesterol acyltransferase activities of the lipoprotein. Remarkably, no studies to date directly quantify either the function or HDL particle distribution of apoA1 recovered from the human artery wall. Methods and Results—A monoclonal antibody (10G1.5) was developed that equally recognizes lipid-free and HDL-associated apoA1 in both native and oxidized forms. Examination of homogenates of atherosclerotic plaque–laden aorta showed >100-fold enrichment of apoA1 compared with normal aorta (P<0.001). Surprisingly, buoyant density fractionation revealed that only a minority (<3% of total) of apoA1 recovered from either lesions or normal aorta resides within an HDL-like particle (1.063≤d≤1.21). In contrast, the majority (>90%) …

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Function And Distribution Of Apolipoprotein A1 In The Artery Wall Are Markedly Distinct From Those In Plasma, Joseph A. Didonato, Ying Huang, Kulwant S. Aulak, Orli Even-Or, Gary Gerstenecker, Valentin Gogonea, Yuping Wu, Paul L. Fox, W.H. Wilson Tang, Edward F. Plow, Jonathan D. Smith, Edward A. Fisher, Stanley L. Hazen Oct 2013

Function And Distribution Of Apolipoprotein A1 In The Artery Wall Are Markedly Distinct From Those In Plasma, Joseph A. Didonato, Ying Huang, Kulwant S. Aulak, Orli Even-Or, Gary Gerstenecker, Valentin Gogonea, Yuping Wu, Paul L. Fox, W.H. Wilson Tang, Edward F. Plow, Jonathan D. Smith, Edward A. Fisher, Stanley L. Hazen

Mathematics and Statistics Faculty Publications

Background—Prior studies show that apolipoprotein A1 (apoA1) recovered from human atherosclerotic lesions is highly oxidized. Ex vivo oxidation of apoA1 or high-density lipoprotein (HDL) cross-links apoA1 and impairs lipid binding, cholesterol efflux, and lecithin-cholesterol acyltransferase activities of the lipoprotein. Remarkably, no studies to date directly quantify either the function or HDL particle distribution of apoA1 recovered from the human artery wall. Methods and Results—A monoclonal antibody (10G1.5) was developed that equally recognizes lipid-free and HDL-associated apoA1 in both native and oxidized forms. Examination of homogenates of atherosclerotic plaque–laden aorta showed >100-fold enrichment of apoA1 compared with normal aorta (P<0.001). Surprisingly, buoyant density fractionation revealed that only a minority (<3% of total) of apoA1 recovered from either lesions or normal aorta resides within an HDL-like particle (1.063≤d≤1.21). In contrast, the majority (>90%) …

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Current, October 07, 2013, University Of Missouri-St. Louis Oct 2013

Current, October 07, 2013, University Of Missouri-St. Louis

Current (2010s)

No abstract provided.

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Bucknell River Reporter, Bucknell University Environmental Center Oct 2013

Bucknell River Reporter, Bucknell University Environmental Center

Newsletters

Volume 1, issue 3 of the Bucknell River Reporter edited by Fred Swader. Articles include -- The River: no news = good news (?), An engineer’s perspective of our local streams and rivers, How Susquehanna river towns deal with flooding, A Fragmented System: Dams in the Susquehanna Watershed, Science, “safety,” and the State of the River, Exploring the river, Watching the river flow, Susquehanna River Initiative.

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Somos & Estamos, Otoño/Fall 2013, Western Michigan University Oct 2013

Somos & Estamos, Otoño/Fall 2013, Western Michigan University

Somos y Estamos

For Friends of the Department of Spanish at Western Michigan University. In both Spanish and English.

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