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Molecular And Functional Characterization Of Bacterial Communities In Organic Farm And Pasture Soils, Sherie L. Edenborn
Molecular And Functional Characterization Of Bacterial Communities In Organic Farm And Pasture Soils, Sherie L. Edenborn
Graduate Theses, Dissertations, and Problem Reports (ETD)
Culture-dependent denaturing gradient gel electrophoresis (CD DGGE) fingerprinting of the 16S rRNA gene was developed to characterize mixed soil bacterial communities recovered on agar plates. CD DGGE analyses resulted in clear banding patterns of sufficient complexity (16--32 major bands) and reproducibility to investigate differences in bacterial communities in silt loam soils. Replicate CD DGGE profiles from plates inoculated with less-dilute samples (10 -3) had a higher band count and were more similar (72--77%) than profiles from more-dilute samples (51--61%). When CD DGGE fingerprints were compared to profiles constructed from 16S rRNA genes obtained from culture-independent clone libraries (CB DGGE profiles) …
Monitor Newsletter March 26, 2007, Bowling Green State University
Monitor Newsletter March 26, 2007, Bowling Green State University
Monitor
Official Publication of Bowling Green State University for Faculty and Staff