Digital Commons Network^™

Oxpat/Pat-1 Is A Ppar-Induced Lipid Droplet Protein That Promotes Fatty Acid Utilization, Nathan E. Wolins, Benjamin K. Quaynor, James R. Skinner, Anatoly Tzekov, Michelle A. Croce, Matthew C. Gropler, Vijayalakshmi Varma, Aiwei Yao-Borengasser, Neda Rasouli, Philip A. Kern, Brian N. Finck, Perry E. Bickel

Clinical and Translational Science Faculty Publications

Lipid droplet proteins of the PAT (perilipin, adipophilin, and TIP47) family regulate cellular neutral lipid stores. We have studied a new member of this family, PAT-1, and found that it is expressed in highly oxidative tissues. We refer to this protein as "OXPAT." Physiologic lipid loading of mouse liver by fasting enriches OXPAT in the lipid droplet tissue fraction. OXPAT resides on lipid droplets with the PAT protein adipophilin in primary cardiomyocytes. Ectopic expression of OXPAT promotes fatty acid-induced triacylglycerol accumulation, long-chain fatty acid oxidation, and mRNAs associated with oxidative metabolism. Consistent with these observations, OXPAT is induced in mouse …

Long-Term Mortality For Older Diabetics Hospitalized With Acute Myocardial Infarction, Marwah Abdalla

Yale Medicine Thesis Digital Library

Diabetics have higher mortality after myocardial infarction (MI), yet little is known regarding the impact of quality of care on long-term survival in older post-MI diabetics. Using data from the Cooperative Cardiovascular Project (CCP), a national cohort of 234,769 Medicare patients aged 65 or older hospitalized with confirmed AMI between 1994-1995, we assessed differences in 10-year mortality outcomes between diabetics and non-diabetics using Cox proportional regression. To account for quality of care, a composite measure among ideal candidates was constructed and entered into the final model adjusting for use of aspirin & beta-blocker on admission/discharge, angiotensin-converting enzyme inhibitors at discharge, …

Diabetes Alters Vascular Mechanotransduction: Pressure-Induced Regulation Of Mitogen Activated Protein Kinases In The Rat Inferior Vena Cava, Kevin M. Rice, Devashish H. Desai, Sunil K. Kakarla, Anjaiah Katta, Deborah L. Preston, Paulette Wehner, Eric R. Blough

MIIR Faculty Research

Background

Diabetes mellitus is an important risk factor for increased vein graft failure after bypass surgery. However, the cellular and molecular mechanism(s) underlying vessel attrition in this population remain largely unexplored. Recent reports have suggested that the pathological remodeling of vein grafts may be mediated by mechanically-induced activation of the mitogen activated protein kinase (MAPK) signaling pathways and the MAPK-related induction of caspase-3 activity. On the basis of these findings, we hypothesized that diabetes may be associated with alterations in how veins "sense" and "respond" to altered mechanical loading.

Methods

Inferior venae cavae (IVC) from the non-diabetic lean (LNZ) and …

The Role Of Body Mass Index And Diabetes In The Development Of Acute Organ Failure And Subsequent Mortality In An Observational Cohort, Katarina Slynkova, David M. Mannino, Greg S. Martin, Richard S. Morehead, Dennis E. Doherty

David M. Mannino

Introduction

Several studies have shown a correlation between body mass index (BMI) and both the development of critical illness and adverse outcomes in critically ill patients. The goal of our study was to examine this relationship prospectively with particular attention to the influence of concomitant diabetes mellitus (DM).

Methods

We analyzed data from 15,408 participants in the Atherosclerosis Risk in Communities (ARIC) study for this analysis. BMI and the presence of DM were defined at baseline. We defined 'acute organ failure' as those subjects who met a standard definition with diagnostic codes abstracted from hospitalization records. Outcomes assessed included the …

Long-Term Trends In Indigenous Deaths From Chronic Diseases In The Northern Territory: A Foot On The Brake, A Foot On The Accelerator, David P. Thomas, John R. Condon, Ian P. Anderson, Shu Q. Li, Stephen Halpin, Joan Cunningham, Steven L. Guthridge

Aboriginal Policy Research Consortium International (APRCi)

Objective: To examine trends in Northern Territory Indigenous mortality from chronic diseases other than cancer. Design: A comparison of trends in rates of mortality from six chronic diseases (ischaemic heart disease [IHD], chronic obstructive pulmonary disease [COPD], cerebrovascular disease [CVD], diabetes mellitus [DM], renal failure [RF] and rheumatic heart disease [RHD]) in the NT Indigenous population with those of the total Australian population.

Participants: NT Indigenous and total Australian populations, 1977–2001. Main outcome measures: Estimated average annual change in chronic disease mortality rates and in mortality rate ratios. Results: DeathratesfromIHDandDMamongNTIndigenouspeoplesincreased between 1977 and 2001, but this increase slowed after 1990. …

Aboriginal Health Workers And Diabetes Care In Remote Community Health Centres: A Mixed Method Analysis, Damin Si, Ross S. Bailie, Samantha J. Togni, Peter H N D'Abbs, Gary W. Robinson

Aboriginal Policy Research Consortium International (APRCi)

Objective: To assess the effect of employing Aboriginal health workers (AHWs) on delivery of diabetes care in remote community health centres, and to identify barriers related to AHWs’ involvement in diabetes and other chronic illness care.

Design, setting and participants: Three-year follow-up study of 137 Aboriginal people with type 2 diabetes in seven remote community health centres in the Northern Territory.

Main outcome measures: Delivery of guideline-scheduled diabetes services; intermediate outcomes (glycated haemoglobin [HbA1c] and blood pressure levels); number and sex of AHWs at health centres over time; barriers to AHWs’ involvement in chronic illness care.

Results: There was a …

Renal Dysfunction, Cardiovascular Risk, And The Response To Ace Inhibition In Patients After Myocardial Infarction, Powell Oliapuram Jose

Yale Medicine Thesis Digital Library

Background: Proteinuria is a known cardiovascular risk factor in hypertensive or diabetic patients as well as the general population. Worsening renal function (WRF) has been shown to influence outcomes in the heart failure population. The prognostic value of these markers for renal dysfunction in patients after myocardial infarction is unclear. Methods: The Survival and Ventricular Enlargement trial (SAVE) randomized 2231 patients with left ventricular dysfunction between 3-16 days (average 11 days) post-MI to receive captopril or placebo; those with a serum creatinine above 2.5mg/dl were excluded from SAVE. WRF was defined as an increase in creatinine greater-than 0.3mg/dl measured from …

The Etiological Role Of Susto In Diabetes Among Hispanics In Southern Nevada, Carin K Wallace

UNLV Retrospective Theses & Dissertations

This thesis explores the association between susto, a Hispanic folk explanation of disease, and type 2 diabetes. This association is explored through a focus group and semi-structured interviews, with participants recruited from a population of 300 diabetes patients under treatment by a local non-profit group. Results show that not only is susto thought to be an important cause of diabetes among Hispanic diabetics and their family members, but that the meaning of susto may be changing; no longer thought of as solely a frightful event, it is a description of the resulting emotions as well. The role of emotions such …

Predictors Of Autonomy Support At Diabetes Summer Camp: A Self-Determination Theory Approach, Ron Ramsing, Jim Sibthorp

Research in Outdoor Education

Diabetes is considered to be one of the most psychologically and behaviorally demanding chronic illnesses facing adolescents (Cox & Gonder-Fredrick, 1992). To control the disease more effectively, adolescents must learn to undertake the management of their diabetes themselves (Mensing, et al., 2000; Ruggiero, et al., 1997). Self-determination theory (Ryan & Deci, 2000) was used as a foundation to explore the mechanisms, within a summer camp, that led to increased perceptions of autonomy support. Research shows autonomy support to be a critical prerequisite for increased autonomous behavior.

With over 350 specialty camps serving youth with diabetes (Diabetes Education and Camping Association, …

Development Of Food Groupings To Guide Dietary Advice In People With Diabetes, L. J. Gillen, Linda C. Tapsell

Faculty of Health and Behavioural Sciences - Papers (Archive)

Foods commonly consumed by 16 adults with diabetes were grouped according to macrinutrient value and type of fat to form 13 categories of which 10 would form the focus of dietary advice. Dietary modeling demonstrated that the food group pattern provided adequate nutrition and low variation in dietary targets. Idealised proportions of fat types were achieved only when daily servings of foods such as oils, nuts, oily fish and soy were included. The food groupings proved appropriate for dietary advice for diabetes.

Pioglitazone Induces Apoptosis Of Macrophages In Human Adipose Tissue, Angela M. Bodles, Vijayalakshmi Varma, Aiwei Yao-Borengasser, Bounleut Phanavanh, Charlotte A. Peterson, Robert E. Mcgehee Jr., Neda Rasouli, Martin Wabitsch, Philip A. Kern

Clinical and Translational Science Faculty Publications

Metabolic syndrome and type 2 diabetes mellitus are associated with an increased number of macrophage cells that infiltrate white adipose tissue (WAT). Previously, we demonstrated that the treatment of subjects with impaired glucose tolerance (IGT) with the peroxisome proliferator-activated receptor γ (PPARγ) agonist pioglitazone resulted in a decrease in macrophage number in adipose tissue. Here, adipose tissue samples from IGT subjects treated with pioglitazone were examined for apoptosis with terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining. TUNEL-positive cells were identified, and there was a significant 42% increase in TUNEL-positive cells following pioglitazone treatment. Overlay experiments with anti-CD68 antibody …

Does Hero Always Help? Differences In Nutrient Intake Among Obese Subjects With And Without Type 2 Diabetes Mellitus Prior To Dietary Intervention, Sze Yen Tan, Lynda J. Gillen, Marijka Batterham, Xu-Feng Huang, Cassandra J. Quick, Yasmine Probst, Serina Faraji, Linda C. Tapsell

Faculty of Health and Behavioural Sciences - Papers (Archive)

Abstract presented at The 2006 Annual Scientific Meeting of the Nutrition Society of Australia, 29 November - 2 December, Sydney, Australia

Digito-Palmar Complex In Non-Insulin Dependent Diabetes Mellitus, Vadgaonkar Rajanigandha, Pai Mangala, Prabhu Latha, Saralaya Vasudha

Turkish Journal of Medical Sciences

Background: It is only recently that the knowledge of dermatoglyphics has been applied to the field of medical and genetic diagnosis. Aim: The aim of the present study was to compare and evaluate the dermatoglyphic pattern in individuals with non-insulin dependent diabetes mellitus (NIDDM) with a normal adult population. Materials and Methods: Both quantitative as well as qualitative parameters were analyzed in 112 (63 male and 49 female) subjects and compared with the data from 142 (65 male and 77 female) healthy controls. Modified Purvis-Smith ink method was used for obtaining the prints, and statistical analyses were done using Student's …

Diabetes Self-Management: Patient Cognition And The Development Of Expertise, Katherine D. Lippa

Browse all Theses and Dissertations

Expert decision making has been widely researched among professionals, but non-professionals make many equally complex decisions. This study uses the case of type II diabetes to explore complex decision making among non-professionals. It was hypothesized that three cognitive aspects of expertise, problem detection, functional relationships, and problem solving, would be linked with higher levels of self-management (higher adherence and lower glucose). Twenty participants with diabetes were interviewed concerning their knowledge and experiences with diabetes. Participants also completed a questionnaire concerning their self-management practices. Interviews were transcribed and thematically coded. Participants who displayed characteristics of expert cognition reported higher levels of …

Effect Of A Single Bout Of Prior Moderate Exercise On Cutaneous Perfusion In Type 2 Diabetes, Sheri R. Colberg, Henri K. Parson, Tanja Nunnold, D. Robb Holton, Aaron I. Vinik

Human Movement Studies & Special Education Faculty Publications

In diabetic individuals, increased shunting of circulation away from the skin may exist, contributing to their greater risk for ulcerations and poor cutaneous healing. In a prospective study (1), we previously found a lower skin perfusion during local heating in the foot dorsum of sedentary type 2 diabetic individuals compared with active people without diabetes. This defect was present despite normal increases in skin interstitial nitric oxide (NO), suggesting that NO is either ineffective or not involved (2). A prior bout of maximal exercise also lessened the impaired responsiveness to local heating of the dorsal foot in active type 2 …

Imaging Islets Labeled Wth Magnetic Nanoparticules At 1.5 Tesla, J.H. Tai, Paula Foster, Alma Rosales, Biao Feng, Craig Ha, Violetta Martinez, Soha Ramadan, Jonatan Snir, C.W. Melling, Savita Dhanvantari, Brian Rutt, David White

Jamie Melling

We have developed a magnetic resonance imaging (MRI) technique for imaging Feridex (superparamagnetic iron oxide [SPIO])-labeled islets of Langerhans using a standard clinical 1.5-Tesla (T) scanner and employing steady-state acquisition imaging sequence (3DFIESTA). Both porcine and rat islets were labeled with SPIO by a transfection technique using a combination of poly-l-lysine and electroporation. Electron microscopy demonstrated presence of SPIO particles within the individual islet cells, including beta-cells and particles trapped between cell membranes. Our labeling method produced a transfection rate of 860 pg to 3.4 ng iron per islet, dependent on the size of the islet. The labeling procedure did …