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Mineralization Of 2-Chlorophenol Using A Hollow Fiber Ultrafiltration System In Combination With A Reactor Containing An Immobilized White Rot Fungus, Sheau-Ming Tung

Theses

Phanerochaete chrysosporium, a white rot fungus was immobilized on a packed-bed reactor, and was used to mineralize an aqueous stream contaminated with 50 ppm of 2-chlorophenol. Hollow fiber ultrafiltration membranes (for 5,000 daltons and 10,000 daltons molecular weight cut-oft) were utilized to retain the extracellular enzyme resulting from the immobilized fungus in a recirculation loop outside of the reactor.

It was found here that the concentration of proteins (roughly proportional to the extracellular enzyme releasd by the fungus) does not correlate with the rate of degradation of 2-chlorophenol in the system. The protein was concentrated 1.5 fold in the recycle …

Inability Of Phanerochaete Chrysosporium To Degrade Polychlorinated Biphenyls In Sand, Pankaj Pankaj

Theses

The white rot fungus Phanerochaete chrysosporium has been previously found to successfully degrade a wide spectrum of chlorinated aromatic hydrocarbons (some of them in soil or sand matrices). In this research, it was tested if the fungus could also degrade a very recalcitrant class of pollutant, i.e. Polychlorinated Biphenyls (PCBs) in sand at a concentration of 100 ppm (w/w PCB to sand). For this purpose, a number of experiments were performed, in which several operating parameters, such as nutrient concentrations (nitrogen and glucose), amount of initial biomass, aeration, and degree of chlorination of the PCBs were varied. The results obtained …

Recalcitrance Of Polychlorinated Biphenyls To Biodegradation By Phanerochaete Chrysosporium In Soil, Thierry B. Poncet

Theses

The white rot fungus Phanerochaete chrysosporium has been shown in the past to effectively degrade a number of chlorinated aromatic compounds. Previous investigators (Eaton, D. C., "Mineralization of Polychlorinated Biphenyls by Phanerochaete chrysosporium, a Lignolytic Fungus", Enz. Microb. Technol., 7, 194-196 (1985); Bumpus, J. A., Tien, M., Wright, D., and Aust, S. D., "Oxidation of Persistent Environmental Pollutants by White Rot Fungus", Science, 228, 1434-1436 (1985)) have also claimed that this fungus is capable of mineralizing polychlorinated biphenyls (PCBs) in submerged cultures under stationary conditions. Therefore, the objective of this work was to study the biodegradation activity of the fungus …