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Improvements In Techniques For Aging Mammals By Dental Cementum Annuli, Douglas S. Allen, Rudy C. Melfi
Improvements In Techniques For Aging Mammals By Dental Cementum Annuli, Douglas S. Allen, Rudy C. Melfi
Proceedings of the Iowa Academy of Science
Several modifications of commonly used histological techniques for the preparation of dental tissues for aging by dental cementum annuli are presented. Improvements in methods of tissue collection, preservation, decalcification, sectioning, and staining permit faster preparation of tissues and produce higher-quality, easily interpreted sections. Possible application of hard-tissue microtomes to sectioning of undecalcified or partially decalcified tissues is also discussed.