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The Actor, The Character, And The Audience: The Impact Of Racial Alignment On Theater Audiences, Conwell Worthington Iii Jan 2026

The Actor, The Character, And The Audience: The Impact Of Racial Alignment On Theater Audiences, Conwell Worthington Iii

Open Access Dissertations

Broadway represents the pinnacle of live theater in the USA, generating billions of dollars in revenue and serving as an international cultural icon. Despite Broadway’s prominence, it faces challenges stemming from its reliance on a relatively homogeneous audience base and a changing market. One determinant of both financial and critical success is casting. Casting also significantly impacts audience perceptions and acceptance. The recent success of Hamilton: An American Musical and Six: The Musical juxtaposed with the failure of 1776 have sparked discussion and interest into how to best utilize non-traditional casting as a strategy to broaden Broadway Audiences. This dissertation …


Generating Template Banks For Intermediate Duration Gravitational Waves, Michael St. Pierre Jan 2026

Generating Template Banks For Intermediate Duration Gravitational Waves, Michael St. Pierre

Open Access Dissertations

There are a multitude of astrophysical sources which carry the potential to produce perturbations in spacetime, known as gravitational waves, across a wide spectrum of frequencies. Over the brief history of gravitational wave astronomy, short-duration “chirp” signals emitted from black hole mergers, highly energetic “burst” signals emitted from supernovae and Gamma-Ray Bursts (GRBs), and long duration “continuous” signals emitted from galactic neutron stars and the stochastic gravitational wave background have traditionally been at the forefront of search development, leaving a class of intermediate duration  ($102 - 104 seconds) gravitational wave signals poorly suited for detection. The conventional way …


Submesoscale Dynamics Of Phytoplankton And Carbon Export Revealed By High-Resolution Airborne And Satellite Remote Sensing Of Currents And Ocean Color, Sarah E. Lang Jan 2026

Submesoscale Dynamics Of Phytoplankton And Carbon Export Revealed By High-Resolution Airborne And Satellite Remote Sensing Of Currents And Ocean Color, Sarah E. Lang

Open Access Dissertations

Satellites and airborne sensors reveal submesoscale (1 - 10 km) variability in ocean color in the form of filaments, eddies, and patches. The variability in ocean color is closely tied to the physical dynamics that restructure phytoplankton distributions and drive active biological responses like changes in primary productivity and community structure. As the base of the marine food web and a key component of the biological carbon pump, phytoplankton are crucial to the overall health of marine ecosystems and to the ocean's role in climate. This dissertation focuses on the use of airborne and satellite remote sensing to study the …


Coarse-Grained Models For Microbial Metabolisms And Biological Interactions, Meng Gao Jan 2026

Coarse-Grained Models For Microbial Metabolisms And Biological Interactions, Meng Gao

Open Access Dissertations

Microorganisms are microscopic, typically unicellular organisms widely distributed across the world’s environments.  In the global ocean, they account for the majority of primary production and decompose organic matter to drive nutrient cycles. Through their metabolic processes, including photosynthesis, dinitrogen (N2) fixation, sulfur oxidation, and methanogenesis, microbes contribute to global biogeochemical processes, and establish their ecological niches in suitable habitats. Their biological interactions, including competition, symbiosis, and grazing, are important links among different functional groups in marine communities. Consequently, quantifying microbial metabolisms and their biological interactions is important to investigate their ecological functions in marine ecosystems and the global …


Unified Bayesian And Machine Learning-Based State Of Charge Estimation In A Pv Battery System, Showrov Rahman Jan 2026

Unified Bayesian And Machine Learning-Based State Of Charge Estimation In A Pv Battery System, Showrov Rahman

Open Access Dissertations

The state of charge (SOC) of a battery indicates the remaining charge in the battery relative to its nominal maximum charge capacity. The accurate estimation of SOC is of utmost importance for efficient energy management as it indicates the level of energy stored in the battery.

Bayesian and machine learning (ML) approaches represent two distinct estimation strategies for battery state estimation. Bayesian filters, such as the Kalman filter (KF) and particle filter (PF), operate sequentially by incorporating physical models of battery dynamics. In contrast, ML-based methods are data-driven, and their performance largely depends on the quantity and quality of training …


Points Of Interest: Implementing Simulation-Based Storm Hazard Consequence Prediction, Samuel W. Adams Jan 2026

Points Of Interest: Implementing Simulation-Based Storm Hazard Consequence Prediction, Samuel W. Adams

Open Access Dissertations

During major ocean storms (hurricanes, tropical storms, nor’easters), coastal communities often experience negative outcomes that local emergency managers (EMs) did not anticipate or sufficiently prepare for. Local EMs often have difficulty obtaining specific, local forecast data and consequence predictions they can act on to prevent losses and minimize avoidable consequences. Despite tremendous advances in forecasting science, uncertainty remains a constant in storm decision making, requiring local EMs to make critical response choices under pressure of limited time and incomplete information. Rising sea levels, more frequent and intense storm events, growing coastal populations, and increasing societal dependence on complex infrastructure are …


Optimizing Poultry Production And Enhancing Egg Nutrient Composition Through Kelp Supplementation In Laying Hens, Meghan Clifford Jan 2026

Optimizing Poultry Production And Enhancing Egg Nutrient Composition Through Kelp Supplementation In Laying Hens, Meghan Clifford

Open Access Dissertations

The development of sustainable feed supplements is a crucial component in addressing the current global need for resilient and environmentally responsible food systems. This dissertation has explored the potential of brown algae (Saccharina latissima) as a sustainable feed supplement for poultry with the potential to benefit production efficiency, product quality, and the global food supply. With both controlled feeding trials and a producer-based survey, the work in this dissertation investigates the nutritional, biological and practical implications of adopting kelp into poultry diets.

Chapter one provides a comprehensive overview of the current literature surrounding the use of marine sources …


Ecce Leadership Development: Supports, Barriers, And Identity Formation, Andrea Weidele Jan 2026

Ecce Leadership Development: Supports, Barriers, And Identity Formation, Andrea Weidele

Open Access Dissertations

Early childhood care and education (ECCE) leadership development is not given the attention it deserves. Despite the proven link between high-quality ECCE programs and positive societal outcomes, leadership development within the sector is significantly under-resourced. Because program quality is inherently tied to leadership efficacy, there is an urgent need to investigate the supports and structures that foster effective ECCE leadership. This study addressed that gap by using a qualitative multiple case study to explore how six ECCE program leaders of licensed ECCE programs from a New England state describe their leadership development, including supports and barriers they encountered and how …


Protective Behavioral Strategies On Alcohol And Cannabis Co-Use Occasions, Sabrina Todaro Jan 2026

Protective Behavioral Strategies On Alcohol And Cannabis Co-Use Occasions, Sabrina Todaro

Open Access Dissertations

Simultaneous use of alcohol and cannabis, or co-use (i.e., the use of both substances so that effects overlap), is an increasingly significant public health concern among young adults between the ages of 18 and 25 in the U.S. (SAMHSA 2025; Terry-McElrath & Patrick, 2018). Protective behavioral strategies (PBS; i.e., tactics used to modify or limit substance use related harm; Pearson, 2013) have been robustly linked to fewer negative outcomes (e.g., lower use quantity, frequency, and consequences) for alcohol- and cannabis-only occasions (Bravo et al., 2016; Bravo et al., 2017; Bravo et al., 2019; Pearson, 2013; Pedersen et al., 2016; Pedersen …


Extending Low-Frequency Traveling-Wave Propagation In A Water-Filled Cylindrical Waveguide Using Active Impedance Control, William Slater Jan 2026

Extending Low-Frequency Traveling-Wave Propagation In A Water-Filled Cylindrical Waveguide Using Active Impedance Control, William Slater

Open Access Dissertations

This dissertation investigates extending the frequency range over which a one-dimensional, plane traveling wave can be generated in a water-filled, steel-walled waveguide under ocean temperature and hydrostatic pressure conditions. The wave guide considered is a thick-walled, steel cylinder filled with a water/glycol mixture with a transducer at each end and a hydrophone array along the length. One transducer excites a plane wave while the other cancels reflections, resulting in a traveling wave in the waveguide. The current (open-loop) measurement system is advertised to generate traveling waves for low frequencies where only 3.4% of a wavelength can be measured (kL …


Linking Phytoplankton Physiology And Ecology To Global Biogeochemical Cycles: A Modeling Approach, Margaret Bernish Jan 2026

Linking Phytoplankton Physiology And Ecology To Global Biogeochemical Cycles: A Modeling Approach, Margaret Bernish

Open Access Dissertations

Phytoplankton, or microscopic primary producers, contribute to carbon export through the biological transformation of carbon dioxide into organic molecules. Phytoplankton are responsible for approximately half of global net primary production, while only contributing minimally to total photosynthetic biomass. Despite their important role in the global cycling of nutrients, phytoplankton physiology and ecology are often highly simplified in global biogeochemical models. This dissertation elucidates the role of phytoplankton physiology and ecology within biogeochemical cycles, allowing for better characterization of marine microbial distribution and better representation of nutrient cycling for interpretive and predictive models. The development of formulations that link physiological responses …


Information Asymmetry: A Configurational Perspective In Buyer-Supplier Relationships, Soode Vaezinejad Jan 2026

Information Asymmetry: A Configurational Perspective In Buyer-Supplier Relationships, Soode Vaezinejad

Open Access Dissertations

Information asymmetry in buyer-supplier relationships has been linked to several influencing factors. However, these factors are often examined in isolation, limiting understanding of their synergistic effects. Grounded in agency theory, signaling theory, and transaction cost economics, this study adopts a configurational perspective to examine which combinations of factors lead to high information asymmetry. Using a three-part study design, we first conduct a systematic literature review to identify relevant factors, which are then refined and prioritized through a Delphi study. Building on this foundation, fuzzy-set Qualitative Comparative Analysis (fsQCA) is applied to survey data from 385 buyer managers to uncover equifinal …


Ultrafast Vibrational Dynamics In Important Wide-Bandgap Semiconductors, Dielectrics And Energetic Materials, Dinusha Madushanka Senarathna Jan 2026

Ultrafast Vibrational Dynamics In Important Wide-Bandgap Semiconductors, Dielectrics And Energetic Materials, Dinusha Madushanka Senarathna

Open Access Dissertations

This dissertation applies time-resolved coherent anti-Stokes Raman scattering (tr-CARS), to measure ultrafast vibrational dynamics in technologically important solid-state and van der Waals molecular systems. The central goal is to use time-domain Raman methods to resolve mode-specific phonon and vibrational dephasing processes with high temporal precision, high spectral selectivity, and sufficient experimental flexibility to probe materials ranging from wide-bandgap semiconductors and oxide crystals to energetic molecular crystals. Across the work, I show that carefully designed tr-CARS measurements can access vibrational lifetimes from hundreds of femtoseconds to several picoseconds, enabling direct observation of decay pathways, relaxation mechanisms, and dispersion effects that shape …


Volatiles In Mantle Sources At Mid-Ocean Ridge And Intraplate Settings, Rebecca Lippitt Jan 2026

Volatiles In Mantle Sources At Mid-Ocean Ridge And Intraplate Settings, Rebecca Lippitt

Open Access Dissertations

Volatiles play an integral role in upper mantle processes and the eruptive styles of volcanoes. Although we know the importance of volatiles to volcanic systems, processes like melting, crystallization, and degassing alter the volatile abundances of mantle melts, making it difficult to quantify mantle source compositions and the extent to which volatiles influence melting processes. Additionally, volatile content may be a key factor in setting the oxidation conditions of mantle source rocks and subsequently changing those conditions in a body of magma as it ascends through the crust and is erupted at the Earth’s surface. Recent work, however, has identified …


Challenges And Best Practices For The Development Of Coastal Hazard Decision Support Tools, Noah Hallisey Jan 2026

Challenges And Best Practices For The Development Of Coastal Hazard Decision Support Tools, Noah Hallisey

Open Access Dissertations

There is growing interest in developing Decision Support Tools (DSTs) supporting emergency managers and other decision-makers during coastal storm events. Yet many tools fail to inform decision-making due to challenges associated with their development and transition into operational tools for real-time decision-making. This dissertation investigates these challenges and offers best practices for their development and implementation for real-world decision-making through a literature review, interviews with subject matter experts in the United States, and a real-world case study.

Manuscript 1 reviews existing published literature to understand whether and how tool developers are incorporating emergency managers in the development of DSTs and …


Assessing Fisheries Dependence Of Coastal Communities In Managed Mangrove Forests In Latin America, Ariel Alexander Quintanilla Magana Jan 2026

Assessing Fisheries Dependence Of Coastal Communities In Managed Mangrove Forests In Latin America, Ariel Alexander Quintanilla Magana

Open Access Dissertations

Fisheries dependency refers to the action of a person, community, or society linking their social, cultural, and economic actions to different ecosystem services (ES) for covering their food system with essential marine species, developing a governance system to conserve these fishing areas, their local ecological knowledge, and guaranteeing food security in the coastal areas. In Latin America, most fishing activities are practiced within three nautical miles of the shoreline, estuaries, lagoons, and mangrove forest channels, using small vessels and traditional gear such as hooks, trammel nets, and gill nets. Some coastal communities have developed their subsistence and livelihood activities in …


Optimizing Markov Chain Monte Carlo Algorithms For Fairness, Scalability, And Interpretability In Electoral Redistricting, Madhukara Kekulandara Jan 2026

Optimizing Markov Chain Monte Carlo Algorithms For Fairness, Scalability, And Interpretability In Electoral Redistricting, Madhukara Kekulandara

Open Access Dissertations

Gerrymandering undermines democratic representation by manipulating electoral district boundaries to dilute the political influence of targeted communities. While Markov Chain Monte Carlo (MCMC) based redistricting algorithms have become a standard computational tool for detecting partisan gerrymandering, their effectiveness in addressing racial gerrymandering and their scalability on modern computing systems remain limited. This dissertation advances the theory and practice of algorithmic redistricting by optimizing MCMC-based approaches across three dimensions: racial fairness, computational efficiency, and interpretability.

First, this work introduces the Partial Map MCMC algorithm, a novel redistricting method designed specifically to address racial gerrymandering under the legal framework of Section 2 …


The Relationship Between Mental Health Disclosures, Employee Sentiments And Turnover Intention In The Workplace, Steve G. Winkel Jan 2025

The Relationship Between Mental Health Disclosures, Employee Sentiments And Turnover Intention In The Workplace, Steve G. Winkel

Open Access Dissertations

Purpose. The purpose of this study is to examine the relationship between employees’ disclosure of mental health conditions in the workplace and these employees’ sentiments, feelings, and associated outcomes. Utilizing constructs of job satisfaction, affective commitment, normative commitment, motivation, and turnover intentions, the research aims to understand quantifiable differences in the employment experience between employees who disclose and do not disclose (non-disclosure) mental health conditions, as well as the mediating roles of human resources management (HRM), supervisors support, and employee authenticity. Through self-reported data detailing employees’ disclosure experiences, the research also aims to understand the disclosure conversation's specific impact …


Monotonic Constant Normal Stiffness Tests For Improving The Skin Friction Resistance Prediction Of Drilled Piles In Sand, Amir Babaee Jan 2025

Monotonic Constant Normal Stiffness Tests For Improving The Skin Friction Resistance Prediction Of Drilled Piles In Sand, Amir Babaee

Open Access Dissertations

This body of work consists of three manuscripts. The first manuscript focuses on the evaluation of constant normal stiffness tests on sand using a newly developed active control system. The second manuscript is related to prediction of axial capacity of drilled and grouted micropiles for offshore foundations. The third manuscript corresponds to evaluation of quality control measures for cyclic resistance of sand using cyclic direct simple shear equipment.

Small diameter drilled piles (micropiles) are being considered for use as an economical foundation for floating offshore wind structures. On land, micropiles are typically load tested to confirm design capacity; this is …


Machine Learning For Optimized Ocean Exploration Data Analysis, Puja Banerjee Jan 2025

Machine Learning For Optimized Ocean Exploration Data Analysis, Puja Banerjee

Open Access Dissertations

The deep sea remains one of the most crucial frontiers in Earth sciences, with vast unknowns regarding its biodiversity, ecosystem dynamics, and geological features and processes, and impact and interaction with humans. Ocean exploration seeks to address this gap by focusing data collection in some of the least known places on the planet in order to significantly expand our knowledge of marine ecosystems, geological formations, and oceanographic processes. Ocean exploration datasets provide crucial insights into a wide range of scientific domains including: deep sea biodiversity, seafloor morphology, and deep-sea soundscapes. The advent of autonomous and remotely operated underwater vehicles (AUVs …


Numerical Modeling Study Of Coastal Impacts From Extreme Storms And Sea Level Rise In Southern New England, Deborah Crowley Jan 2025

Numerical Modeling Study Of Coastal Impacts From Extreme Storms And Sea Level Rise In Southern New England, Deborah Crowley

Open Access Dissertations

Coastal areas in southern New England are vulnerable to a range of impacts from extreme storms and sea level rise. The most frequent extreme storms are extratropical storms, commonly referred to as nor’easters, named for the direction of the strongest winds, though these storms also have strong winds from other directions.

Storm winds generate large waves and push water up along the coast in the form of storm surge. The combined impacts of increased water levels and waves include flooding and morphological change. Sea level rise (SLR) will exacerbate these processes, amplifying the associated impacts, which may pose increasing threats …


Dynamics Of Metal-Coordinated Coumarin Dyes For Photoswitching Applications, Danielle Jacoby Jan 2025

Dynamics Of Metal-Coordinated Coumarin Dyes For Photoswitching Applications, Danielle Jacoby

Open Access Dissertations

Optical transient absorption spectroscopy is a fundamental pump-probe technique that is used to analyze the photophysical and photochemical properties of molecules. Our research group specifically utilizes both a femtosecond and nanosecond set up to probe dynamics of molecules on time scales ranging from 100s of femtoseconds to approximately 50 microseconds. Chapter 2 is a standard operating procedure for the nanosecond transient absorption instrument.

Photoacids are molecules that undergo a change in the acidity of one of their protons when exposed to light. Utilizing this phenomenon, we have designed a series of dyes that, when bound to divalent first row transition …


“Swap What No Longer Serves”: An Exploration Of Zero Waste Fashion Communities Online And Irl, Rosemary Leger Jan 2025

“Swap What No Longer Serves”: An Exploration Of Zero Waste Fashion Communities Online And Irl, Rosemary Leger

Open Access Dissertations

As sustainability concerns grow within the fashion industry, consumer-based communities play a critical role in shaping more responsible consumption practices. This dissertation explores how individuals engage with zero-waste fashion both online and in physical practice, offering a deeper understanding of their motivations, behaviors, and visions for a more sustainable fashion future.

Through a two-study approach, this research investigates the communication and knowledge-sharing practices within digital zero-waste fashion communities and explores the experiences of individuals participating in offline zero-waste initiatives. Study 1 employs netnography to analyze online zero-waste fashion discourse, identifying how consumers exchange knowledge and promote sustainable practices in digital …


Development And Characterization Of Polysaccharide-Based Controlled Drug Delivery Platforms, Amanda Pepler Jan 2025

Development And Characterization Of Polysaccharide-Based Controlled Drug Delivery Platforms, Amanda Pepler

Open Access Dissertations

Drug delivery research focuses on overcoming challenges in patient treatment. Most therapeutics are delivered systemically through oral or intravenous routes. However, these administration methods are often inefficient due to therapeutic hydrophobicity, poor bioavailability, and liver metabolic clearance, necessitating frequent dosing. When treatment plans require frequent dosing, patients experience significant fluctuations in therapeutic concentration in the bloodstream, which can increase adverse side effects. These factors contribute to low patient compliance. Therefore, the core focus of drug delivery research is to develop new administration routes for therapeutics. Drug delivery systems (DDS) that can target therapeutics to a localized region offer advantages over …


Textual Sediments: Genre And The Planetary Novel, William Bowden Jan 2025

Textual Sediments: Genre And The Planetary Novel, William Bowden

Open Access Dissertations

This dissertation investigates how twenty-first-century prose fiction navigates the ecological and temporal dimensions of planetary crisis. In response to accelerating pressures - climate change, ecological collapse, and the intensification of capitalist modernity - literary studies has turned increasingly to the planetary as a conceptual frame. Yet this shift has often outpaced the development of adequate critical vocabularies and narrative methodologies. By drawing on ecocriticism, narrative theory, phenomenology, and related fields, Textual Sediments proposes a new framework for reading what it terms the planetary novel: a sedimentary genre characterized by temporal layering, formal hybridity, and a recursive engagement with scale. Through …


Quantifying Mixotrophy Potential As A Function Of Environmental And Biological Drivers In Coastal And Estuarine Plankton Communities, Erica Ewton Jan 2025

Quantifying Mixotrophy Potential As A Function Of Environmental And Biological Drivers In Coastal And Estuarine Plankton Communities, Erica Ewton

Open Access Dissertations

The discovery of mixotrophic plankton has bridged gaps in nutrient cycling budgets and altered the understanding of food webs and energy transfer. Mixotrophic plankton are capable of simultaneous or sequential photosynthesis and heterotrophy, providing multiple avenues of nutrition and thus reducing limitations that affect specialists. These energy dense cells provide increased biomass for higher trophic levels, which may increase organism sizes throughout the food web. Additionally, they may be physically denser, which alters biogeochemical cycling including carbon sequestration. The work presented here uses LysoTracker Green, an acidotropic stain that attaches to phagocytic vacuoles within potentially phagotrophic small (< 20 µm diameter) phytoplankton communities (‘mixoplankton’), to better understand their abundance, relative abundance, and growth rates in relation to the matrix of natural conditions they inhabit. Understanding the prevalence and drivers of mixoplankton is vital to better parameterize biogeochemical cycling and energy transfer in the ocean and for models that predict the fate of future oceans.In the first study, I compared the abundance of mixoplankton in the Northeast U.S. Shelf (NES) and Coastal California Current System (CCS) during two cruises in the NES in summer 2021 and one cruise in the CCS in summer 2022. These two contrasting coastal systems span the breadth of environmental conditions observed in temperate marine ecosystems and thus provided an opportunity to examine indicators of mixoplankton across temperate coastal regions. I discovered higher mixoplankton abundance in the NES than CCS yet similar population sizes of nanoeukaryotic photosynthesizers, resulting in higher relative abundance of mixoplankton in the NES than CCS. Across both systems, I found increased mixoplankton abundance associated with decreasing temperature and combined nitrate + nitrite concentrations (N+N), and increasing light intensity and Synechococcus abundance …


Investigating The Photophysical Dynamics Of Anthracene And Acridine Derivatives Using Time-Resolved Spectroscopy, Sophia Tiano Jan 2025

Investigating The Photophysical Dynamics Of Anthracene And Acridine Derivatives Using Time-Resolved Spectroscopy, Sophia Tiano

Open Access Dissertations

Anthracenes and acridines are among the simplest visible light absorbing heterocyclic and polycyclic aromatic hydrocarbons and as such are extensively used in a variety of applications including photosensitizers and fluorescent sensors. Although the photophysics of some derivatives have been studied in the past, there is still a lot of unknown fundamental information that is vital in order to optimize these molecules for particular applications. Transient absorption spectroscopy was used to study the photophysics of the 9-substituted methyl, hydroxymethyl and formyl derivatives of acridine and anthracene. The growth and decay of the triplet-triplet absorption peak were quantified for each dye in …


Stability And Global Dynamics Of Autonomous And Nonautonomous Discrete Population Models With Stocking, Harvesting, And Cooperation, Sam Habach Jan 2025

Stability And Global Dynamics Of Autonomous And Nonautonomous Discrete Population Models With Stocking, Harvesting, And Cooperation, Sam Habach

Open Access Dissertations

This dissertation investigates the local and global behavior of several classes of discrete population models involving stocking, harvesting, and cooperation. The study is divided into three major manuscripts, each offering new theoretical in sights, bifurcation results, and applications to real-world data.

In Manuscript 1, we study the Beverton-Holt population models with constant and proportional stocking and harvesting, as well as sigmoid Beverton-Holt models under constant stocking and harvesting, both in autonomous and non-autonomous settings. We analyze stability and explore global dynamics using established local and global dynamics theorems, bifurcation theory, and explicit solutions when available. Special attention is given to …


The Influence Of The Greatest 8tm Text Message Service On Parental Involvement In Children’S Mental Health, Rebecca Michel Jan 2025

The Influence Of The Greatest 8tm Text Message Service On Parental Involvement In Children’S Mental Health, Rebecca Michel

Open Access Dissertations

The present study examined the impact of The Greatest 8Ô text messaging service on parental involvement in promoting children’s mental health and wellness. A quasi-experimental design compared 63 subscribers and 115 non-subscribers of the service. Parents completed an online survey assessing confidence, engagement, beliefs about eight core mental health skills, and perceived child improvement, along with exploratory measures regarding information sources, preferred communication methods, and barriers to accessing resources. It was hypothesized that subscribers would report higher confidence, engagement, and beliefs than non-subscribers, that duration in the program would predict these outcomes, and that engagement would moderate the relationship between …


Ultra-Rapid Electrophilic Cysteine Arylation Using Pyridinium Salts, Bradley M. Lipka Jan 2025

Ultra-Rapid Electrophilic Cysteine Arylation Using Pyridinium Salts, Bradley M. Lipka

Open Access Dissertations

Small molecule-based bioconjugation enables in vitro and in vivo functionalization of proteins and peptides, significantly expanding the chemical space of biomacromolecules. Among these transformations, nucleophilic aromatic substitution (SNAr) of cysteine residues is a widely employed strategy for protein bioconjugation.

Although many reagents facilitate cysteine arylation, they are disadvantageous in two crucial aspects - limited synthetic modularity and undesired hydrophobicity. First, conventional cysteine arylating reagents are frequently activated by multiple electron-withdrawing groups (EWGs) on a single aromatic ring, leaving few synthetic handles to derivatize the reagents for advanced applications. Second, these labeling reagents are generally hydrophobic in nature and …