Digital Commons Network^™

Coral Holobiont Lipidome Response To Global Change, Lauren Zane

Open Access Dissertations

Climate change is fundamentally restructuring macro-scale marine ecosystem structure and function by altering microscale metabolic processes and patterns of energy allocation. Scleractinian corals, as major ecosystem engineers, play outsized roles in ecosystem structure and function. They also occupy diverse habitats that differ in temperature regimes, light environments, hydrodynamics, and other environmental conditions, exposing corals to substantial spatial variation in stress and resource availability. Their fragile nutritional symbiosis with algal endosymbionts helps them navigate these environmental variations, but are also highly sensitive to environmental stress, particularly the timing and magnitude of water temperature changes. As pressure from environmental changes increases, the …

Assessing Fisheries Dependence Of Coastal Communities In Managed Mangrove Forests In Latin America, Ariel Alexander Quintanilla Magana

Open Access Dissertations

Fisheries dependency refers to the action of a person, community, or society linking their social, cultural, and economic actions to different ecosystem services (ES) for covering their food system with essential marine species, developing a governance system to conserve these fishing areas, their local ecological knowledge, and guaranteeing food security in the coastal areas. In Latin America, most fishing activities are practiced within three nautical miles of the shoreline, estuaries, lagoons, and mangrove forest channels, using small vessels and traditional gear such as hooks, trammel nets, and gill nets. Some coastal communities have developed their subsistence and livelihood activities in …

Optimizing Poultry Production And Enhancing Egg Nutrient Composition Through Kelp Supplementation In Laying Hens, Meghan Clifford

Open Access Dissertations

The development of sustainable feed supplements is a crucial component in addressing the current global need for resilient and environmentally responsible food systems. This dissertation has explored the potential of brown algae (Saccharina latissima) as a sustainable feed supplement for poultry with the potential to benefit production efficiency, product quality, and the global food supply. With both controlled feeding trials and a producer-based survey, the work in this dissertation investigates the nutritional, biological and practical implications of adopting kelp into poultry diets.

Chapter one provides a comprehensive overview of the current literature surrounding the use of marine sources …

Characterization Of Methodological Variability In Compound Specific Isotope Analysis Of Amino Acids (Csia-Aa), Audreyana Brown-Nash

Open Access Dissertations

The use of isotopes in the natural sciences stems from the principle that chemical reactions predictably alter the isotopic composition of a given sample. Recent advances in compound specific isotope analysis of amino acids (CSIA-AA) have paved the way for studies of increasingly complex ecological relationships, including the identification and characterization of organismal physiology, consumer-resource linkages, biogeochemical cycling, and movement ecology. The analytical power of CSIA-AA has caused an exponential increase in the number of application-based studies since its inception in the 1990s. With the technique constantly evolving to accommodate new research goals, it is important to understand how variation …

Ultra-Processed Food Intake And Dietary Intake Behaviors In Young Adults, Alexandra Black Larcom

Open Access Dissertations

Ultra-processed food (UPF) intake has been positively associated with adverse health outcomes and poor diet quality (DQ). UPFs tend to be of lower nutritional quality (NQ) than less processed foods, though NQ varies widely within each category of food processing. Young adults are the highest consumers of UPF, but UPF intake and related behaviors have not been examined in college students, a key subset of young adults. UPF overconsumption at meals has been proposed as a mechanism for the association between UPF intake, poor DQ, and adverse health outcomes, though no existing studies have considered NQ. Further, consumer understanding of …

Molecular Interactions Between The Shellfish Pathogen Vibrio Coralliilyticus Re22 And The Probiont Phaeobacter Inhibens S4sm, Jessica Coppersmith

Open Access Dissertations

Oyster larvae aquaculture is an important industry, both economically and environmentally, helping to create sustainable food systems by supplying seed oysters to farms, and also to restoration projects aimed at building coastal resilience and improving water quality by rejuvenating wild populations. However, oyster larvae are highly susceptible to rapid mortality events, crashes, which can seriously impact the industry. One source of these crashes in hatchery systems is vibriosis, caused by pathogenic species of Vibrio bacteria that are ubiquitous in temperate coastal waters, and with rising surface water temperatures their prevalence and virulence are increasing. To prevent these larval crashes from …

Quantifying Links Between Phytoplankton Physiology And The Environment, Gabrielle M. Armin

Open Access Dissertations

Phytoplankton are microscopic, primary producers in aquatic ecosystems worldwide. They are key links in global biogeochemical cycles, converting inorganic compounds into bioavailable nutrients which fuel fisheries. Through photosynthesis, phytoplankton play an essential role in the carbon cycle and marine biological pump, drawing carbon from the atmosphere, which is eventually sequestered in the oceans, regulating our Earth’s climate. Due to their influential role in large scale processes, there have been many efforts to characterize and quantify phytoplankton impacts to ecosystem health and global nutrient cycles. Often, phytoplankton physiology has been highly simplified in global or ecosystem models, limiting their representation to …

More Than Just Food And Money: An Exploration Of College Students’ Challenges With Food Access And Insecurity, Orianna Duncan Carvalho

Open Access Dissertations

There has long been a popular prototypical image of the starving college student surviving on cheap ramen noodle meals, but research suggests there is more to this than meets the eye. Food insecurity, the condition in which individuals are unable to secure enough food to support a healthy lifestyle, has been investigated and reported with increasing frequency on college campuses, including the university at which the present study is conducted. Thus, the purpose of the present study was to delve further into college student food insecurity to gain a better understanding of how students experience and perceive it. To achieve …

The Effect Of Maternal Diet On Developmental Programming: A Cross Species Analysis, Lindsey Eileen Eaton

Open Access Dissertations

Maternal nutrition plays an important role in shaping offspring health through developmental programming, with lasting implications on metabolic function and disease susceptibility. This dissertation explores the molecular and physical consequences of maternal nutrition, particularly its effects on pancreatic development and function in offspring. Chapter one provides a comprehensive review of the literature, detailing the mechanisms of fetal development, epigenetic regulation, and metabolic programming. It highlights the thrifty phenotype hypothesis, the role of DNA methylation and histone modifications, and the impact of maternal nutrition, emphasizing how nutrient availability during pregnancy influences fetal organogenesis and long term metabolic health.

Chapter two examines …

Intersections Of Inattention: A Qualitative Inquiry Into The Experiences Of Multiply Marginalized Students In Community College Settings, Jana N. Knibb

Open Access Dissertations

Educational research has examined the impact of expanded access to higher education for historically marginalized groups. However, research on the experiences of students who are both racially minoritized and dis/abled is limited. Findings suggest that, due to their intersectional identities, multiply marginalized dis/abled students (MMDS) navigate policies and practices related to racist ableism, which further marginalize them and create additional barriers in their higher education experiences. Expanding this research requires theoretical frameworks that account for students' identities, experiences, and barriers in higher educational spaces and contexts. This qualitative study, grounded in Dis/ability Studies and Critical Race Theory in Education (DisCrit), …

Quantifying Mixotrophy Potential As A Function Of Environmental And Biological Drivers In Coastal And Estuarine Plankton Communities, Erica Ewton

Open Access Dissertations

The discovery of mixotrophic plankton has bridged gaps in nutrient cycling budgets and altered the understanding of food webs and energy transfer. Mixotrophic plankton are capable of simultaneous or sequential photosynthesis and heterotrophy, providing multiple avenues of nutrition and thus reducing limitations that affect specialists. These energy dense cells provide increased biomass for higher trophic levels, which may increase organism sizes throughout the food web. Additionally, they may be physically denser, which alters biogeochemical cycling including carbon sequestration. The work presented here uses LysoTracker Green, an acidotropic stain that attaches to phagocytic vacuoles within potentially phagotrophic small (< 20 µm diameter) phytoplankton communities (‘mixoplankton’), to better understand their abundance, relative abundance, and growth rates in relation to the matrix of natural conditions they inhabit. Understanding the prevalence and drivers of mixoplankton is vital to better parameterize biogeochemical cycling and energy transfer in the ocean and for models that predict the fate of future oceans.In the first study, I compared the abundance of mixoplankton in the Northeast U.S. Shelf (NES) and Coastal California Current System (CCS) during two cruises in the NES in summer 2021 and one cruise in the CCS in summer 2022. These two contrasting coastal systems span the breadth of environmental conditions observed in temperate marine ecosystems and thus provided an opportunity to examine indicators of mixoplankton across temperate coastal regions. I discovered higher mixoplankton abundance in the NES than CCS yet similar population sizes of nanoeukaryotic photosynthesizers, resulting in higher relative abundance of mixoplankton in the NES than CCS. Across both systems, I found increased mixoplankton abundance associated with decreasing temperature and combined nitrate + nitrite concentrations (N+N), and increasing light intensity and Synechococcus abundance …

Per- And Polyfluoroalkyl Substances (Pfas) Effects On Tissue Disposition, Proteome, And Health Effects, Juliana Agudelo Areiza

Open Access Dissertations

Per- and polyfluoroalkyl substances are a class of nonbiodegradable synthetic aliphatic environmental toxicants that are ubiquitous in the environment. A small subset, specifically perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA), have been designated as highly hazardous to human health by the Environmental Protection Agency (EPA) under the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA). PFOA and PFOS-associated adverse health effects include, but are not limited to, immune suppression, thyroid dysfunction, increased serum cholesterol, liver damage (i.e., alanine aminotransferase (ALT)), and developmental toxicity (i.e., low fetal birth weight and neurotoxicity). In this doctoral dissertation, it was hypothesized that environmental PFAS …

A Socio-Ecological Investigation Of Ulithian Spearfishing, Joshua Pi

Open Access Dissertations

Coral reef socio-ecological systems (SESs) characterize the interconnected relationships between coral reef ecosystems and reef-dependent communities. One such SES exists in Ulithi Atoll, Federated States of Micronesia, where Ulithians fish, steward, and govern their reefs through customary marine tenure, traditional ecological knowledge, and cultural taboos. In recent decades, rapid modernization in Ulithi has introduced new fishing technologies, transformed fishing practices, and ultimately reshaped the functioning of Ulithi’s traditional SES. One significant change was the introduction of spearfishing during the mid-20^th century, which is more selective and individualistic compared to other community-centric fishing methods that have existed for generations. This …

“What’S Supp” Developing An In Vitro Model For Healthy Oral Biofilms, Alex Labossiere

Open Access Dissertations

The study of oral plaque has largely been influenced by the study of pathogens. Here in Chapter 1, I will discuss how this pathogenic focus has been dominant until the onset of microbiome research and begin to explain microbiomes in the context of supragingival plaque in homeostatic communities. From there we will then discuss the possible roots where dysbiosis may arise from plaque to cause disease and ways in which we can study healthy supragingival plaque by in vitro models.

In chapter 2, I will discuss molecular commensalism and how it can be viewed as a tool to further understand …

"Understanding Obesity Experiences And Treatment Decision-Making In Hispanic-Latino Communities”, Viviane Fornasaro-Donahue

Open Access Dissertations

Obesity disproportionately affects Hispanic-Latino adults in the United States, yet rates of metabolic and bariatric surgery (MBS) remain lower in this population compared with non-Hispanic White adults. This dissertation comprises three interconnected phases aimed to (I) explore perceptions of obesity, obesity treatment, and patient-provider communication, (II) inform the development of a culturally responsive bilingual educational material about obesity and MBS, and (III) evaluate its acceptability among Hispanic-Latinos adults with obesity.

Phase I involved a qualitative exploration using semi-structured interviews with Hispanic-Latino adults (n=26) recruited from a community and a clinical MBS setting. These interviews explored participants’ perceptions of …

Improving The Diet Quality Of Wic-Participating Women Using Digital Food Environments, Yarisbel Melo Herrera

Open Access Dissertations

Maternal diet plays a critical role during the perinatal period, impacting both short- and long-term health outcomes in women and their children. Lower-income individuals are at higher risk of having a low diet quality. Therefore, the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) aims to reduce the negative impacts of low socioeconomic status on the diet of its participants (low-income pregnant and postpartum women and children up to age 5) to promote optimal short- and long-term health outcomes. Numerous studies show that WIC improves children’s diet quality, but associations in women have been insufficiently evaluated. While there …

Racial And Ethnic Disparities In Hospital Practices That Support Breastfeeding In The United States, Jane Lazar Tucker

Open Access Dissertations

Breastfeeding is the optimal form of infant nutrition and has many health benefits for mothers as well as infants (CDC, 2022a; Victora et al., 2016). Though rates of breastfeeding have improved in recent years, disparities in breastfeeding persist among Black and Hispanic women compared to White women (Beauregard et al., 2019; Chiang et al., 2021), despite similar intentions to breastfeed across all racial/ethnic groups (Hamner et al., 2021). Hospital practices that support breastfeeding, such as the baby being placed skin-to-skin immediately after delivery or staying in the room with the mother throughout the hospital stay, are known to predict breastfeeding …

Effects Of Western Diet Or Exercise Exposure In A Novel Rat Model Of Late-Onset Alzheimer’S Disease, Jennifer M. Mattar

Open Access Dissertations

Sporadic, late-onset Alzheimer’s disease (LOAD) accounts for a staggering 98% of cases yet does not arise from a single genetic mutation. Rather, an extensive body of research has revealed that LOAD is likely the result of complex interactions between genetics and several lifestyle factors. There exists a substantial gap in understanding the mechanisms behind LOAD development and the impacts of lifestyle on disease progression. Enhanced rodent models may help elucidate mechanisms by which LOAD develops. This longitudinal study introduces a novel rat model of LOAD (CrAβ), created using CRISPR-Cas9 gene editing technology to express humanized Aβ. The effects of a …

Implementation And Evaluation Of A Nutriton-Focused Steam Summer Program For Urban Youth, Jacquelyn Potvin

Open Access Dissertations

Background: Youth from low-income, racially and ethnically diverse families are at increased risk for academic challenges and nutritionally inadequate diets. Summer programs offer an opportunity to foster interest and engage students in science, technology, engineering, art, and math (STEAM) education while addressing summer learning loss. Nutrition education early in life can help youth develop healthy dietary behaviors that they carry into adulthood. Project stRIde: Science and Technology Reinforced by Innovative Dietary Education was a summer program for 4-6th grade youth from racially and ethnically diverse and low-income families. The goal of the program was to engage youth in nutrition-centered …

Mechanistic And Toxicokinetic Analysis Of Perfluoroalkyl Substances: Proteomics Approach And The Role Of Fatty Acid Binding (Fabp), Seyed Mohamad Sadegh Modaresi

Open Access Dissertations

Per- and polyfluoroalkyl substances (PFAS) are persistent chemicals used in many industries, known for their resilience and resistance to degradation. The extended half-lives of PFAS in the human body result in bioaccumulation, with serum half-lives for perfluorooctanoic acid (PFOA), perfluorooctane sulfonic acid (PFOS), and perfluorohexane sulfonic acid (PFHxS) estimated at 3.4, 4.6, and 7.1 years, respectively. PFAS primarily accumulate in the liver, hypothesized due to their resemblance to fatty acids and their binding with liver fatty acid-binding proteins (LFABP), affecting lipid metabolism and leading to liver toxicity. Although extensive research has been performed on long-chain PFAS over the past decade, …

Advancing Clinical-Community Partnerships To Address Pediatric Food Insecurity, Nader Hamdi

Open Access Dissertations

Background: Children and families experiencing low income in the United States are at a greater risk of food insecurity (FI) and suboptimal diet quality than the general population. Childhood FI, which is associated with numerous poor health and developmental outcomes, has become so pervasive that the American Academy of Pediatrics recommends universal FI screenings. Routine FI screening in clinical settings presents opportunities for clinic-community partnerships to connect families experiencing FI with food access and nutrition education (FANE) programs. A variety of FANE programs, including the Supplemental Nutrition Assistance Program - Education (SNAP-Ed), are administered at the federal level to …

Learning From The Past: Environmental Impacts On Coral Performance, Reproduction, And Epigenetic Inheritance, Danielle M. Becker

Open Access Dissertations

Coral reef ecosystems face extreme challenges due to increasing ocean temperatures caused by anthropogenically induced global climate change and require reproductive replenishment for reef persistence. Corals and other marine invertebrates have exhibited the capacity for environmental memory, or the retention of information from prior environmental experiences, which can be inherited by subsequent generations (e.g., via non-genetic mechanisms). However, less is known about how molecular modifications in the coral epigenome influence acclimatization and adaptive responses to environmental variation within and across generations. Here, I present three manuscripts testing different mechanisms involved in coral’s physiological, reproductive, and epigenetic response to environmental perturbation …

Evaluation Of The Toxicokinetic Mechanisms Of Perfluoroalkyl Substances: Accumulation, Distribution, And Elimination, Sangwoo Ryu

Open Access Dissertations

Per- and polyfluoroalkyl substances (PFAS) are a diverse class of anthropogenic chemicals associated with several adverse health outcomes in humans. The accumulation of these PFAS have been examined over several decades, however, the elucidation of the toxicokinetics of these chemicals are still in its infancy. The distribution and elimination mechanisms of specific PFAS, notably perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA), have undergone comprehensive investigation, however, there are more than 10,000 currently detected PFAS in the environment that have not been examined. It is, therefore, imperative to not only address individual PFAS but also to encompass overarching trends and correlations …

13c Labeling For Cho Cell Metabolism Tracing And Ms Based Analysis For Advanced Upstream Culture Monitoring To Support Cqa Understanding, Xin Bush

Open Access Dissertations

Monoclonal antibodies (mAbs) make up a large part of biopharmaceutical sales due to their great success in treating cancer, autoimmune, and cardiovascular diseases. Due to an increasing number of mAbs using innovative approaches in drug development, combined with changes in the drug market, there is an increased need for optimizing process intensification and quality control. Thus, a profound understanding of cellular metabolism and how this impacts the protein product’s critical quality attributes (CQA) would be of great value. Among the quality attributes of mAbs, post-translational modifications such as N-linked glycosylation are of particular importance as they affect the activity, efficacy, …

Cell Stress, Protein Quality Control, And Activation Of Toxin Antitoxin Systems In Escherichia Coli, Benjamin Piraino

Open Access Dissertations

Responding to environmental stress is critical for all organismal survival, including in bacteria. One of the key bacterial responses to stress is protection of proteins within the cell, which helps to maintain proteins in a functional state. Protection of the proteome is done through a collaborative network of proteins and systems, all functioning to protect the cell. Within Escherichia coli (E. coli), three main proteomic protection systems are chaperones, proteases, and toxin-antitoxin (TA) systems. They work together, with overlapping functions, to regulate stress responses. Chaperones, such as DnaK(Hsp70) and SecB, are major regulators of protein folding within the cell, …

Associations Between Stress, Intuitive Eating, And Adiposity In A Cohort Of Midlife Women, Bridget A. Owens

Open Access Dissertations

Stress and eating behaviors can influence the health of midlife women, a population at risk for weight gain and redistribution of fat to the abdominal area related to shifts in reproductive hormones and aging. Higher stress is associated with negative health outcomes such as increased abdominal adiposity, depression, and chronic diseases such as cardiovascular disease. Women report higher stress and more emotional and physical side effects from stress than men and are more likely than men to engage in emotional eating in response to stress. Further, the desire to lose weight and the prevalence of body dissatisfaction is high among …

Hypoglycemia Screening In Term Normal Birth Weight Newborns: A Retrospective Electronic Health Record Review, Michelle G. Palmer

Open Access Dissertations

A gap in the evidence exists regarding screening of term normal birth weight newborns without identified risk factors for the presence of hypoglycemia. The purpose of this retrospective electronic health record review was to examine factors leading to and results associated with blood glucose screening of term normal birth weight newborns without previously identified risk factors for hypoglycemia.

The specific aims of this study were to: (a) determine if there were statistically significant differences between screened and unscreened full term, normal birth weight newborns without pre-existing identified risk factors for hypoglycemia by: gender, race, delayed cord clamping, skin-to-skin initiation and …

Best Management And Egg Washing Practices For Small-Scale Poultry Producers To Improve Food Safety, Julie Bosland

Open Access Dissertations

The goal of this dissertation research was to determine the best management and egg washing practices for small-scale egg producers and backyard chicken owners to help improve food safety. The main goals were to establish the most effective management and egg washing procedures to reduce total coliform bacteria load on eggshells and increase egg cleanliness. To determine the methods used for experiments in chapters 3-5, a survey was conducted on egg handling, washing and management techniques in backyard poultry and small-scale producers within the Northeastern U.S. The effect of management practices and egg washing methods on the eggshell cuticle was …

Associations Between Oyster Consumption And Iron, Zinc, And Cadmium Concentrations In Ghanaian Women, Alyssa Abreu

Open Access Dissertations

Background: Oysters are an excellent source of iron and zinc but may be contaminated by heavy metals such as cadmium. The micronutrient and metal content of smoked, dried oysters as well as the impact of oyster consumption on iron, zinc, and cadmium concentrations in Ghana is unknown.

Objectives: Our objectives were to 1) determine the micronutrient and metal content and health risk of dried, smoked oysters, 2) determine the associations between dietary iron intake with hemoglobin or ferritin concentrations and anemia, 3) determine the associations between oyster consumption and iron, zinc and cadmium concentrations in Ghanaian women living near the …

Cranberry Vine And Its Effects On The Periparturient Ovine And Her Offspring, Delaney Smith

Open Access Dissertations

Gastrointestinal nematodes (GIN) are a major health concern globally, particularly for small ruminant producers. The most deadly of these GIN is an anemia-inducing parasite called Haemonchus contortus. Infections of H. contortus are most severe during what's known as the periparturient period (PPP), which is the last 6 weeks of gestation and first 8 weeks of lactation in maternal animals. Widespread genetic resistance of H. contortus to the traditionally used chemical dewormers have led producers and researchers alike to turn to the plant kingdom for answers. Plant based compounds have been used as bioactive compounds for centuries to cure a …