Digital Commons Network^™

Investigating The Effects Of Nucleopore Defects On The Cellular Response To Stress In Als/Ftd Ipsc-Derived Neurons, Alicia Christine Collins

Open Access Dissertations

Amyotrophic lateral sclerosis/frontotemporal dementia (ALS/FTD) is a progressive neurodegenerative disease spectrum characterized by motor dysfunction, cognitive decline, and death within a few years of clinical onset. Hallmark features of ALS/FTD include defects in nucleocytoplasmic transport (NCT) and abnormal cytoplasmic mislocalization and aggregation of the RNA-binding protein TDP-43, both of which have been shown to be exasperated by oxidative stress (OS). It remains unclear how these processes are connected and how they collectively contribute to neurodegeneration. To fill this gap of knowledge, we used human embryonic kidney cells, neuroblastoma cells, and induced pluripotent stem cell-derived cortical neurons harboring the ALS/FTD C9ORF72 …

Using An Ecological And Cultural Lens To Examine The 1st Year Persistence And Science Identity Development Of At-Risk Biology Students Experiencing Multiple Equity-Minded High Impact Practices, Aura Fajardo Grandidge

Open Access Dissertations

In this study, I used an ecological and a cultural perspective to examine the first-year experiences of two cohorts of biology students exposed to multiple equity-minded High Impact Practices (HIPs) designed to promote their persistence and science identity development.

In chapter 1, I applied Graham et al.’s Persistence Framework (2013) and Bronfenbrenner’s Ecological Systems Theory (1977) to investigate the bridges and barriers to persistence that biology students face while experiencing either an equity minded learning community, a first-year seminar, a course-based research experience, and/or an active learning introductory biology course. Using a mixed methods approach that focused on phenomenology, I …

An Investigation Of Alkb-Family Dna Repair Enzymes And Their Interaction With Synthetic, Modified Oligonucleotides, Samuel Dean Howarth

Open Access Dissertations

Chapter One will detail the synthesis, purification, and characterization of DNA oligonucleotides containing one of fifteen modified nucleic acid bases. For each modification, relevant background information is provided to highlight its biological significance. This chapter primarily focuses on the development of oligonucleotide stocks for use in future biochemical and cellular assays. Several of these modifications are recognized substrates of AlkB-family enzymes, while others remain uncharacterized as potential substrates.^1-4 The discovery of novel substrates for AlkB-family enzymes can be facilitated through comprehensive in vitro biochemical assays, contingent upon the availability of well-characterized substrate stocks. The methods and findings presented here …

The Impact Of A Curricular Change On Undergraduate Human Anatomy And Physiology Students’ Academic Behavior And Performance, Jessica A. Adams

Open Access Dissertations

This dissertation explores learning in context. Undergraduate introductory human anatomy and human physiology courses are either taught as discipline-specific or integrated anatomy and physiology (A&P) sequences. These courses are critically important to future health care professionals yet a consensus is lacking on the optimal course approach. Our institution underwent a curricular revision to change pedagogical methodologies from discipline-specific human anatomy and human physiology to an integrated A&P I and II sequence. Thus, providing a unique opportunity to study the potential impact of contextual learning. Specifically, over three years the effects integrating physiological concepts had on anatomy education were explored as …

Early Turn-Taking Prediction For Human Robot Collaboration, Tian Zhou

Open Access Dissertations

To enable natural and fluent human robot collaboration, it is critical for a robot to comprehend their human partners’ on-going actions, predict their behaviors in the near future, and plan its actions accordingly. Specifically, the capability of making early predictions can allow robots to determine the precise timing of turn-taking events and start planning and executing preparative tasks to take the turn. Such proactive behavior would save waiting time and increase efficiency and naturalness in collaborative tasks.

To that end, this dissertation presents the design and implementation of an early turn-taking prediction framework, centered around physical human robot collaboration tasks. …

Quantifying Human Heat Stress In Working Environments, And Their Relationship To Atmospheric Dynamics, Due To Global Climate Change, Jonathan R. Buzan

Open Access Dissertations

Heat stress is a global issue that crosses socioeconomic status. Heat stress leads to reduced worker capacity on seasonal scales, and weekly to sub-daily timescales, incapacitation, morbidity, and mortality. This dissertation focuses on 2 distinct parts: quantification methods of heat stress, and heat stress applications. Quantification methods of heat stress Chapters 1–3 focus on historical analysis of heat stress. Chapter 1 is a detailed assessment of previous work in heat stress—methods, history, and future research outlook. Chapter 2 focuses on the implementation and quantification of a battery of heat stress metrics within the global circulation model framework. The ultimate outcome …

Functional Organization Of The Brain At Rest And During Complex Tasks Using Fmri, Lauren Kelly Lynch

Open Access Dissertations

How and why functional connectivity (FC), which captures the correlations among brain regions and/or networks, differs in various brain states has been incompletely understood. I review high-level background on this problem and how it relates to 1) the contributions of task-evoked activity, 2) white-matter fMRI, and 3) disease states in Chapter 1. In Chapter 2, based on the notion that brain activity during a task reflects an unknown mixture of spontaneous activity and task-evoked responses, we uncovered that the difference in FC between a task state (a naturalistic movie) and resting state only marginally (3-15%) reflects task-evoked connectivity. Instead, these …

Virtual Environments For The Induction Of Action, Brian Adrian Flowers

Open Access Dissertations

This dissertation is a study on the ways in which Virtual Reality can actively and reactively affect human physiology. The first manuscript describes the design of a highly generalized Virtual Reality biofeedback system. This system was developed such that, given proper design, it would be able to provide users with a highly responsive Virtual Environment to meet the criteria of embodiment, which will increase the user's responsiveness to stimuli. The system also functions as a biofeedback system that can be used for biomechanical as well as physiological feedback by using a generalized design paradigm.

The second manuscript describes a biofeedback …

Lin28/Let-7 Axis As A Regulator Of Myocardial Ischemic Injury, Shaurya Joshi

Open Access Dissertations

One of the prominent causes of heart failure is atherosclerosis of the coronary arteries. Obstruction of blood flow within the coronary arteries results in inadequate perfusion, or ischemia, of the heart muscle. The let-7 family of microRNAs (miRs) regulates critical cell functions, including survival signaling, metabolic control and glucose utilization, which may be important during myocardial ischemia. MiR-let-7 expression is under tight temporal and spatial control through multiple redundant mechanisms. The mechanisms and functional consequences of miR-let-7 regulation in ischemic myocardium largely remain unknown. Here we show that miR-let-7c, -7a and -7g are downregulated in the adult mouse heart early …

Modeling And Fundamental Design Considerations For Portable, Wearable And Implantable Electronic Biosensors, Piyush Dak

Open Access Dissertations

Chronic diseases such as cancer, diabetes, acquired immune deficiency syndrome (AIDS), etc. are leading causes of mortality all over the world. Portable, wearable and implantable biosensors can go a long way in preventing these premature deaths by frequent or continuous self-monitoring of vital health parameters.

Nanoparticle Depot For Intraperitoneal Chemotherapy Of Ovarian Cancer, Bo Sun

Open Access Dissertations

Intraperitoneal (IP) chemotherapy is a promising post-surgical therapy of ovarian cancer, with the full potential yet to be proven. To facilitate IP chemotherapy of ovarian cancer, we have developed a nanoparticle depot for IP chemotherapy consisted of paclitaxel (PTX) nanocrystals (PNC) and hyaluronic acid-based hydrogel (HA gel). PNC with a size of ~310 nm was produced by nonsolvent and temperature-induced crystallization. Dissolution kinetics of PNC could be determined by the light scattering method rather than the dialysis method due to drug reprecipitation caused by diffusion barrier. PTX release profiles from PNC-gel and PTX precipitate-gel (PPT-gel) were estimated in both sink- …

Model-Based 3-D Reconstruction Of Transparent Objects From A Single Image, Wiehrs Lewis Collier

Open Access Dissertations

This dissertation describes an interdisciplinary research project that spans computer-vision, imaging of biological nanoparticles via cryogenic transmission electron microscopy (cryo-tem), and bio-informatics / proteomics. This research introduces a new method for reconstructing individual biological particles imaged via cryo-tem with typical accuracies of 88% to 98%. This individual particle reconstruction (IPR) technique is based on a hidden Markov model derived from the underlying biological structure. The processing presented in this dissertation reconstructs each individual particle from a single image, which is in contrast to current approaches that can require tens of thousands of images to create an ensemble representation …

Development Of Isotags For Nmr Based Metabolite Profiling And Applications, Fariba Tayyari

Open Access Dissertations

NMR spectroscopy is a powerful analytical tool for both qualitative and quantitative metabolite profiling analysis. However, accurate quantitative analysis of biological systems especially using one dimensional NMR has been challenging due to signal overlap. In contrast, the enhanced resolution and sensitivity offered by chemoselective isotope tags have enabled new and enhanced methods for detecting hundreds of quantifiable metabolites in biofluids using NMR spectroscopy or mass spectrometry. In this thesis we show improved sensitivity and resolution of NMR experiments imparted by 15N and 13C isotope tagging which enables the accurate analysis of plasma metabolites. To date, isotope tagging has been used …

A Study Of Microtubule Dipole Lattices, Shubhendu Nandi

Open Access Dissertations

Microtubules are cytoskeletal protein polymers orchestrating a host of important cellular functions including, but not limited to, cell support, cell division, cell motility and cell transport. In this thesis, we construct a toy-model of the microtubule lattice composed of vector Ising spins representing tubulin molecules, the building block of microtubules. Nearest-neighbor and next-to-nearest neighbor interactions are considered within an anisotropic dielectric medium. As a consequence of the helical topology, we observe that certain spin orientations render the lattice frustrated with nearest neighbor ferroelectric and next-to-nearest neighbor antiferroelectric bonds. Under these conditions, the lattice displays the remarkable property of stabilizing certain …

On Design And Implementation Of An Embedded System For Neural-Machine Interface For Artificial Legs, Xiaorong Zhang

Open Access Dissertations

According to limb loss statistics, there are over one million leg amputees in the US whose lives are severely impacted by their conditions. In order to improve the quality of life of patients with leg amputations, neural activities have been studied by many researchers for intuitive prosthesis control. The neural signals collected from muscles are electromyographic (EMG) signals, which represent neuromuscular activities and are effective bioelectrical signals for expressing movement intent. EMG pattern recognition (PR) is a widely used method for characterizing EMG signals and classifying movement intent. The key to the success of neural-controlled artificial limbs is the neural-machine …

Energetic Salts: Degradation And Transformation, Sravanthi Vadlamannati

Open Access Dissertations

The first part of this work mainly focuses on distinguishing and characterizing two white solids namely urea nitrate (UN) and nitrourea (NU) with similar melting point (~160oC). Urea Nitrate is an inorganic salt. It has found use as an improvised explosive and was used in the first world trade center bombings. Nitrourea, a dehydrated product of UN, is an organic salt. In this study we reported and compared two routes to NU synthesis. We also proposed a decomposition route for UN.

The second part of the work investigates perchlorate contamination in soils following fireworks displays. A total of two hundred …

The Effects Of N-Butonol On The Model And Reconstituted Membrane Of Clostridium Pasteurianum, Yogi Kurniawan

Open Access Dissertations

During n-butanol fermentation, n-butanol partitions into microbial membranes result in destabilizing cellular lipid membranes by altering their lipid composition (generally the ratio of saturated to unsaturated lipids) whereas adapted microorganisms respond by altering the ratio of unsaturated to saturated lipids. The mechanism of how microbes achieve a high adaptation in response to n-butanol is barely known. This dissertation describes the role of unsaturated lipids and charged lipid composition in modulating n-butanol partition into membrane using model bacteria (i.e. lipid bilayer vesicles or liposomes and Langmuir monolayers) and those studies were compared with reconstituted membranes (Clostridium pasteurianum) that represented …

Model-Based Approaches To Characterize Clinical Pharmacokinetics Of Atorvastatin And Rosuvastatin In Disease State, Joyce Macwan

Open Access Dissertations

Cardiovascular disorders are the leading cause of death in the United States. Members of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitors (statins) class of lipid-lowering drugs are used worldwide for the prevention and treatment of cardiovascular disorders. Cardiovascular disorders are the primary cause of morbidity and mortality in patients with diabetes mellitus and obesity as well as renal and liver transplantation. The rising global burden of these chronic disorders has resulted in long-term use of statins to prevent and treat cardiovascular disorders in diverse patient populations. Several statins are commercially available; however, atorvastatin calcium (Lipitor^®, Pfizer Pharmaceuticals, NY) is the …

Asleep Behind The Wheel: Experiences Of Night Shift Nurses On The Commute Home, Shirley A. Sargent

Open Access Dissertations

Aim: The aim of this study was to obtain descriptions of nurses’ perceptions and experiences of falling asleep behind the wheel on the drive home after a worked night shift.

Background: While the evidence suggests that the problem of drowsy driving and fall asleep driving is a strong contributor to vehicular accidents, little is known about nursing perceptions of the issue.

Methods: The design of the study is qualitative and descriptive. Face-to-face interviews were conducted with 12 registered nurses who had experienced falling asleep behind the wheel on the drive home from work after a worked night shift. The interviews …

Micrornas As Effectors For Acetyltransferase P300 In Cardiac Hypertrophy, Salil Sharma

Open Access Dissertations

Myocardial transcriptional response to stress is critically dependent on availability of the histone acetyltransferase p300. p300 is a transcription coactivator which is rapidly induced by hemodynamic stress in the myocardium. This induction is both necessary and sufficient to drive myocardial hypertrophy in vivo. Although short-term elevation of p300 may be adaptive, sustained elevation of p300 increases the risk of heart failure. The mechanism of p300 induction during stress is unknown. The downstream effectors of p300 have been only incompletely elucidated. In this context, the role of microRNAs in regulating p300-driven cardiac hypertrophy can be of potential interest. MicroRNAs are short …

Psychophysiological Responses To Disgust: Cardiovascular And Facial Muscle Patterns Associated With Different Functional Domains, Robert Oum

Open Access Dissertations

This study examined the distinguishing physiological characteristics of the disgust reaction across different domains. According to an evolutionary analysis, disgust is a heterogeneous emotion with features that are specific to three distinct domains: pathogens, sex, and morality. Each domain is predicted to take as input information specific to the adaptive problem it evolved to solve and regulate behavior accordingly. The goal of the present study was to investigate whether there are any adaptive physiological differences associated with the disgust response across domains. Participants were asked to imagine acts that elicit pathogen, sexual, and moral disgust. It was hypothesized that there …

Characterizing The Advanced Glycation Endproducts Formation Of Proteins And Nucleotides By Methylglyoxal And Glyoxal, Praveen Kumar Pampati

Open Access Dissertations

Advanced glycation endproducts (AGEs) are a heterogeneous group of molecules formed from the nonenzymatic reaction of reducing sugars with free amino groups of proteins, lipids, and nucleic acids. The initial product of this reaction is called a Schiff base, which rearranges itself into an Amadori or glycation product which can further rearrange to AGEs. The reaction is called Maillard reaction and is the basis for all protein glycation studies. AGEs accumulate in tissues and associate with the pathology of various diseases like Diabetes, Alzheimer's disease, renal failure, aging, etc. Methylglyoxal, 3-Deoxyglucosone and glyoxal (oxoaldehydes or α-dicarbonyls) have recently been proposed …

Body Burden Of Pahs And Cardiovascular Disease In The United States, John D. Clark Iii

Open Access Dissertations

Polycyclic aromatic hydrocarbons (PAHs) are environmental and occupational carcinogens that are produced by the incomplete combustion of organic material, such as from the burning of tobacco, coal, and petroleum products. In addition to causing cancer, exposure to PAHs is hypothesized to contribute to atherosclerosis and to lead to increased incidence rates of cardiovascular disease in populations. Considering the number of deaths attributable to tobacco smoke exposure, ambient air pollution, and occupational hazards, PAHs may be a significant contributor to the prevalence of cardiovascular disease in human populations. However, a clear exposure-response relationship between PAHs and measures of cardiovascular disease has …

A Sequential Algorithm For Biological Event Detection Using Statistical Nonstationarity, John Dicecco

Open Access Dissertations

High dimension complex dynamical systems, such as those found in physiological processes, produce time series which are often accompanied by nonstationarity. In many cases, the nonstationarity is caused by a physiologically significant event such as the prelude to ventricular fibrillation in cardiac arrest or the change of stasis by introducing pharmaceuticals. A need exists to be able to detect and monitor this change. Most conventional attempts at addressing this problem involve segmenting the time series and evaluating the statistics of the segments. The difficulty with this approach is that the nature of the nonstationarity can be transient, such that it …

Study On The Nonenzymatic Glycation Of Nucleosides/Nucleotides And Proteins With Sugars: An In Vitro Investigation Of Advanced Glycation Endproducts (Ages) Formation, Yuyuan Li

Open Access Dissertations

Advanced glycation endproducts (AGEs) are a complex and heterogeneous group 1 of compounds formed by the nonenzymatic reactions of the carbonyl groups of reducing sugars with the free amino groups of biomolecules. Nonenzymatic reactions can occur in vitro and in vivo and affect the structure, function and conformation of many biomolecules, and AGEs has been suggested to associate with the pathogenesis of various disease such as diabetes and Alzheimer disease. The increased number of reports on the nonenzymatic glycation of proteins and nucleic acids has generated great interest in the methods for characterization of AGEs. The lack of universally applicable …

A Framework For The Creation Of A Unified Electronic Medical Record Using Biometrics, Data Fusion And Belief Theory, Dwayne C. Leonard

Open Access Dissertations

The technology exists for the migration of healthcare data from its archaic paper-based system to an electronic one and once in digital form, to be transported anywhere in the world in a matter of seconds. The advent of universally accessible healthcare data benefits all participants, but one of the outstanding problems that must be addressed is how to uniquely identify and link a patient to his or her specific medical data. To date, a few solutions to this problem have been proposed that are limited in their effectiveness. We propose the use of biometric technology within our FIRD framework in …

Analyses Of Parent And Teacher Responses On The Stress Survey Schedule For Children With Autism Spectrum Disorders, Lisa A. Femia

Open Access Dissertations

To date, the Stress Survey Schedule (SSS) is the only survey that comprises items that are highly relevant to problems often experienced by people with Autism Spectrum Disorders (ASDs). Information regarding the validity of this instrument, however, is limited. The current study collected information from parents and teachers of children between the ages of 3-18 with a primary diagnosis of ASD from multiple sites using the SSS. Convergent and divergent validity estimates were examined using Pearson product-moment correlations coefficients framed in a multi-trait--multi-method matrix. In addition, reference samples were developed for teacher and parent ratings, providing means and standard deviations …

Requirement Of Integrin α5β1 And Tyrosine Phosphorylation Of Shc For Prohb-Egf Release By Gpr30, A Seven Transmembrane Receptor For Estrogen, Jeffrey Alan Quinn

Open Access Dissertations

Estrogen acts via a seven transmembrane receptor (7TMR), GPR30, to stimulate an HB-EGF autocrine loop. Here, evidence is provided that integrin α5β1 is a necessary transmembrane signaling intermediary required for matrix metalloproteinase (MMP)-mediated release of proHB-EGF by GPR30. Estrogen stimulation of human SKBR3 breast cancer cells, which lack ERα and ERβ, resulted in recruitment of integrin α5β1 to focal adhesions, formation of actin stress fibers, and assembly of fibronectin (FN) fibrils. Expression of a GPR30 mutant in SKBR3 cells selectively abrogated FN matrix assembly and EGFR tyrosine phosphorylation by estrogen. Disruption of cytoskeletal integrity or inhibition of integrin α5β1 with …

Advanced Glycation Endproducts Analysis Of Glucosamine With Reducing Sugars, Dna Nucleosides And Serum Proteins, Udayan Dutta

Open Access Dissertations

Advanced glycation endproducts (AGE) are a complex and heterogeneous group of compounds formed by the nonenzymatic reactions of reducing sugars with amino acids and proteins. The reaction is called the Maillard reaction and is central to all protein glycation studies. Accumulation of AGE in human tissue has been associated with the pathology of various diseases like diabetes, Alzheimer's disease, renal failure and ageing and has generated great interest in methods for characterizing the glycated products. Glucosamine GlcN is an unregulated high profile dietary supplement used for the treatment of osteoarthritis with few side effects. There is very little understanding on …

Forensic And Biological Applications Of Vibrational Spectroscopy, Edita Botonjic

Open Access Dissertations

Determination of the age of a bloodstain can be used to establish the time when a crime was committed. As blood ages, deoxyhemoglobin (HbO 2) is converted into methemoglobin (MetHb), which is evident by the color change from red to brown. Fluctuations in temperature will cause blood to age at a non-constant rate. It has been shown that higher temperatures cause deoxyhemoglobin to oxidize more rapidly to methemoglobin. Human blood from vein and capillary vessels was used in this investigation. The samples were prepared by placing blood on various substrates such as gauze and watch glasses. Mid-IR and near-IR spectra …