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Understanding The Kinetics Of Laser-Induced Nanowelding Of Nanoparticles And The Motility Of Bacteria When Faced With Obstacles, Ariel Rogers
Graduate Theses and Dissertations
This dissertation has two focus areas: nanoparticle nanowelding and bacteria motility in the presence of micrometer sized structures. There are two main projects for the nanoparticle nanowelding studies: “Real-time imaging of laser-induced nanowelding in solution” and “Two-color laser-induced nanostructure shape modulation.” For the real-time imaging project, I used a fluorescence microscope, a 405 nm laser, and various python packages to quantify the average size of nanowelded nanostructures as a function of time and found that the average nanostructure growth over time fit the parameters of A¯(t) ∝ c0(1−e−t/τ), where c0 represents the initial concentration of nanoparticles in the solution and …
Toxicity Of Different Insecticides To Sitophilus Oryzae, Tribolium Castaeneum And Tribolium Confusum Infesting Corn, Amina Twaibu
Toxicity Of Different Insecticides To Sitophilus Oryzae, Tribolium Castaeneum And Tribolium Confusum Infesting Corn, Amina Twaibu
Graduate Theses and Dissertations
Corn is an important cereal crop cultivated across various countries, including the United States. Corn serves multiple purposes such as human and livestock food and raw material for industrial processes. Despite being an important crop, corn production faces challenges due to factors such as weather fluctuations and the presence of insect pests and diseases, both in the field and during storage. Among these challenges, insect infestation poses a major threat, as the insect damage not only reduces the quantity and quality of the grain but also renders it unfit for human and animal consumption, leading to a decrease in market …
Isolation Of Drug-Sensitive Eimeria Species From Wild Turkey Feces And Development Of A Model Bioshuttle Program For Eimeria Meleagrimitis For Domestic Turkeys, Maria Carolina Trujillo Peralta
Isolation Of Drug-Sensitive Eimeria Species From Wild Turkey Feces And Development Of A Model Bioshuttle Program For Eimeria Meleagrimitis For Domestic Turkeys, Maria Carolina Trujillo Peralta
Graduate Theses and Dissertations
The objective of this thesis was to determine the anticoccidial profile of Eimeria spp. derived from wild turkeys. These drug-sensitive Eimeria spp. have potential to be used as vaccine candidates to control coccidiosis in commercial turkeys. Chapter two consists of a brief literature review focused on coccidiosis in turkeys. Chapter three addresses the methodologies utilized to isolate, recover, and speciate Eimeria recovered from wild turkey feces in the eastern two-thirds of the United States. From this, we successfully obtained single oocyst-derived stocks for E. meleagrimitis, E. dispersa, E. meleagridis, E. gallopavonis, and E. adenoeides. Chapter four describes the experiment conducted …
Identification Of Genomic, Proteomic, And Metabolomic Signatures Associated With Pulmonary Hypertension Syndrome In Broilers, Duaa Almansaf
Identification Of Genomic, Proteomic, And Metabolomic Signatures Associated With Pulmonary Hypertension Syndrome In Broilers, Duaa Almansaf
Graduate Theses and Dissertations
The present dissertation contains a collection of studies that examine the genomic, proteomic, and metabolomic association to pulmonary hypertension or ascites phenotype in fast-growing broilers. Pulmonary hypertension is a multifactorial metabolic disease influenced by physiological, environmental, and nutritional factors. It is characterized by a number of structural changes including, thrombosis and adverse pulmonary vascular remodeling. Thus, the atrial pressure is increased, and the right ventricle becomes hypertrophied, resulting in heart failure and the death of the bird. Pulmonary hypertension or ascites is a global problem that has negatively impacted the economy. The increased mortality rate of broilers (25%) is estimated …
Systematics Of Eastern North American Podothrombium (Parasitengona: Podothrombiidae), Kelsey Lynn Cline
Systematics Of Eastern North American Podothrombium (Parasitengona: Podothrombiidae), Kelsey Lynn Cline
Graduate Theses and Dissertations
Velvet mites of the genus Podothrombium Berlese, 1910 are found worldwide with only two described species known from North America, both occurring on the west coast. The present study describes five new species occurring in the east, from both larval and post-larval stages. Species hypotheses are supported with morphology and analysis of mitochondrial (barcoding region of COI) and nuclear genes (D2-3 expansion regions of 28S rDNA). Specimens are incorporated from the Barcode of Life Data Systems (BOLD), allowing our dataset to span most of North America. Therefore, we were able to raise the number of Podothrombium in North America from …
Phenotypic Plasticity Of Japanese Medaka Gill In Response To Changing Salinities, Laura V. Ellis
Phenotypic Plasticity Of Japanese Medaka Gill In Response To Changing Salinities, Laura V. Ellis
Graduate Theses and Dissertations
Japanese medaka (Oryzais latipes) are euryhaline fish, meaning they are capable of surviving in a variety of salinities from fresh water to seawater. The ability to maintain an internal osmotic concentration stems from the phenotypic plasticity of the osmoregulatory organs, the gill, kidney, intestine, and integument. The gill is the main site of osmotic and ionic regulation in fish due to the three-dimensional structure, the direct contact with the outside environment, and the composition of the gill cells. Fish gills are multifunctional as they regulate water movement, acid/base exchange, nitrogenous waste excretion, and ion fluctuations. In freshwater environments, fish are …
Aglycon Modifications Of Ipomoeassin F: Synthetic Route Development And Analog Synthesis To Enable Further Sar Studies, Eric Barber
Graduate Theses and Dissertations
The ipomoeassin family of resin glycosides were discovered to have a high potency against numerous cancer cell line, with ipomoeassin F being the most potent among the family of natural products. Interestingly, one of the few differences between ipomoeassin F and the other compounds is the length of the fatty-acid derived aglycon. As the mechanism of action for this family of resin glycosides is unknown and didn’t have any significant COMPARE correlation with the recorded anticancer agents in the National Cancer Institute (NCI), further SAR studies are needed. Drawing on the differences between ipomoeassin F and the other ipomoeassins, it …
Species And Cultural Management Of Earthworms On Golf Course Turf In Arkansas And Oklahoma, Paige Elizabeth Boyle
Species And Cultural Management Of Earthworms On Golf Course Turf In Arkansas And Oklahoma, Paige Elizabeth Boyle
Graduate Theses and Dissertations
As earthworms feed, they can egest soil and nutrient-rich aggregates (casts) on the soil surface. In low-cut turfgrass systems, such as golf course greens, tees, and fairways, surface casting can result in a muddy playing surface, ball roll issues, weed and pest invasion, reduced aesthetics, surface softening, and reduced photosynthesis. Because the use of pesticides for earthworm control is illegal in the U.S., earthworm casting must be managed through cultural practices. Sand topdressing is one method of earthworm control studied for use on golf courses, with the supposition being that the abrasive sand particles will deter the soft-bodied earthworms from …
Immunostimulatory Effects Of Antigen-Conjugated Inp/Zns Quantum Dot Nanoparticles In An Avian Model, Christopher Lyle
Immunostimulatory Effects Of Antigen-Conjugated Inp/Zns Quantum Dot Nanoparticles In An Avian Model, Christopher Lyle
Graduate Theses and Dissertations
Due to their unique physicochemical and enhanced immunostimulatory properties, quantum dot (QD) nanoparticles have shown increasing promise in biomedical research applications including bioimaging, drug delivery, and as vaccine adjuvants. Toxicity, however, remains a concern for the use of QD in these applications and thus, there is an increased demand for effective in vitro and in vivo systems to measure the bioactivity of QD. In this study in vitro and in vivo chicken models were used to investigate the effects of QD on innate and adaptive immunity. Chicken macrophage cultures were treated in vitro with QD to measure macrophage activation and …
Developing Algorithms For Quantifying The Super Resolution Microscopic Data: Applications To The Quantification Of Protein-Reorganization In Bacteria Responding To Treatment By Silver Ions, Sai Divya Challapalli
Developing Algorithms For Quantifying The Super Resolution Microscopic Data: Applications To The Quantification Of Protein-Reorganization In Bacteria Responding To Treatment By Silver Ions, Sai Divya Challapalli
Graduate Theses and Dissertations
Histone-like nucleoid structuring proteins (HNS) play significant roles in shaping the chromosomal DNA, regulation of transcriptional networks in microbes, as well as bacterial responses to environmental changes such as temperature fluctuations. In this work, the intracellular organization of HNS proteins in E. coli bacteria was investigated utilizing super-resolution fluorescence microscopy, which surpasses conventional microscopy by 10–20 fold in spatial resolution. More importantly, the changes of the spatial distribution of HNS proteins in E. coli, by addition of silver ions into the growth medium were explored. To quantify the spatial distribution of HNS in bacteria and its changes, an automatic method …
Analysis Of Ac/Ds Activation Tagged Mutants In Tomato (Solanum Lycopersicum), Ipeleng Randome
Analysis Of Ac/Ds Activation Tagged Mutants In Tomato (Solanum Lycopersicum), Ipeleng Randome
Graduate Theses and Dissertations
Tomato (Solanum lycopersicum) is a crop of immense economic and nutritional importance worldwide and also a good model organism for genomic studies of other dicot species. The recent completion of the tomato genome sequence is a great milestone towards learning about the tomato genome. Elucidation of the function of the different genes using different functional genomic tools is therefore important in adding to this resource. To this end, we have developed an Ac-Ds transposon ‘activation tagging’ (ATag) system to be able to transpose transposon inserts, bearing a strong 35S-enhancer element, all around the genome. An Ac-Ds ATag construct was used …
Fret Study Of Ligand Binding And Exchange Kinetics On The Surface Of Cdse/Zns Quantum Dots, Randee Jean Mcbride
Fret Study Of Ligand Binding And Exchange Kinetics On The Surface Of Cdse/Zns Quantum Dots, Randee Jean Mcbride
Graduate Theses and Dissertations
Quantum dots are a valuable tool in many research applications and commercial development. Their applications are far reaching and as such have become a topic of great interest. One of their greatest assets is the ability to utilize them as water soluble fluorescent labels. Rendering the nanocrystals water soluble can be accomplished in several ways, but one very popular method is by capping them with water-soluble multi-functional organic ligands. However, these ligands, when attached to the quantum dot surface, are labile and can be exchanged which can be problematic when using the quantum dots in research applications. This projects investigates …
Understanding The Influence Of Interfacial Chemistry In Core, Core/Shell And Core/Shell/Shell Quantum Dots On Their Fluorescence Properties, Omondi Bernard Omogo
Understanding The Influence Of Interfacial Chemistry In Core, Core/Shell And Core/Shell/Shell Quantum Dots On Their Fluorescence Properties, Omondi Bernard Omogo
Graduate Theses and Dissertations
Colloidal semiconductor nanocrystals (quantum dots) have received a great deal of attention due to their superior size tunable properties and promising applications in many areas. Some of the most practical areas of their applications include light emitting diodes (LED), photovoltaic and biological studies. Synthetic methods of these crystals is becoming more established with new strategies being reported every now and then. However, quantitative studies connecting the processes at the interface, namely core-ligand, core-shell and shell-shells, to the overall quantum dots fluorescence properties are not well understood. Specifically for cores, relating surface-atoms interactions, solvents, ligands nature, density and functional groups on …
Proteomic Studies Of The Influenza Virus-Human Cell Interactions: The Responses Of Host Cell Protein Expression To Viral Infection And The Novel Host Proteins That Interact With Virus Protein Ns1, Yimeng Wang
Graduate Theses and Dissertations
Influenza A viruses (IAVs) continue to be a threat to human health. Despite extensive studies, the mechanisms underlying the IAVs-host interactions during IAV infection remain elusive. We employed quantitative proteomic methods to systematically explore the host cell protein expression responses to IAV infection and examine the function of a critical IAV protein called NS1 by identifying its host binding partners. Specifically, we used a 2-dimentional gel electrophoresis (2-DE) based proteomic method to screen host proteins whose expression was substantially altered by IAV. One critical protein named IκB kinase-gamma (IKKγ) was found to be significantly down-regulated during IAV infection. Functional studies …
Interactions Between Ions And Lysenin Nanochannels And Their Potential Applications As Biosensors, Radwan Awwad Al Faouri
Interactions Between Ions And Lysenin Nanochannels And Their Potential Applications As Biosensors, Radwan Awwad Al Faouri
Graduate Theses and Dissertations
Lysenin is classified as a pore-forming toxin protein that is isolated from the earthworm Eisenia fetida and consists of 297 amino acids [1]. Lysenin inserts large conducting pores (3.0-4.7 nm in diameter) into artificial membranes (BLM) which include sphingomyelin. These pores (channels) are open and oriented upon insertion into the bilayer lipid membrane. Lysenin channels gate at positive voltages (voltage-induced gating), but not at negative voltages. Lysenin pores also exhibit activity modulation in response to changes in ionic strength and pH, indicating that electrostatic interaction is responsible for Lysenin conductance activities. In this line of inquiries, and by modulating Lysenin …
Characterization Of Novel Histone H2b Mutants Associated With Chromosome Segregation Defects In Saccharomyces Cerevisiae, Thiruchelvam Rajagopal
Characterization Of Novel Histone H2b Mutants Associated With Chromosome Segregation Defects In Saccharomyces Cerevisiae, Thiruchelvam Rajagopal
Graduate Theses and Dissertations
Histones are small basic proteins that associate with DNA to form the basic unit of chromatin, the nucleosome. Histones H3 and H4 form a tetramer that is bound by two H2A-H2B dimers to form the histone octamer, to which approximately 146 bp of DNA wrap around to form the nucleosome. High resolution structural information and recent advances in the understanding of histone post-translational modifications have illuminated the many regulatory functions chromatin exerts in the cell, from the transcriptional control of gene expression to chromosome segregation. However, the specific role that histones play in these processes is not well understood. Previous …
Characterization And Functional Regulation Of Bioactive Peptides In Avian Macrophages And Heterophils, Lakshmi Kannan
Characterization And Functional Regulation Of Bioactive Peptides In Avian Macrophages And Heterophils, Lakshmi Kannan
Graduate Theses and Dissertations
Oligopeptides and low molecular weight polypeptides play central roles as effectors and signal transducers acting as hormones, neurotransmitters, growth factors, toxins, and antimicrobial factors that are important for the survival of the organism. Owing to the ubiquitous involvement of peptides in many key regulatory processes, we have been interested to identify native peptides in different cells and tissues and understand their functions. To conduct our studies, we used avian macrophages and heterophils as models of specialized cells which constitute central components of innate immunity. These studies involved (a) qualitative identification and characterization of the peptides associated with high intensity mass …
Neisseria Gonorrhoeae Prib: A Probe To The Mechanisms Of Bacterial Dna Replication Restart Pathways, Jinlan Dong
Neisseria Gonorrhoeae Prib: A Probe To The Mechanisms Of Bacterial Dna Replication Restart Pathways, Jinlan Dong
Graduate Theses and Dissertations
No abstract provided.
Cooperative Mobile Target Capture Using Artificial Potential And Sliding Mode Control, Jingyi Yao
Cooperative Mobile Target Capture Using Artificial Potential And Sliding Mode Control, Jingyi Yao
Graduate Theses and Dissertations
No abstract provided.
Cooperative Navigation Of Autonomous Vehicles In A Known Environment, Shreecharan Kanchanavally
Cooperative Navigation Of Autonomous Vehicles In A Known Environment, Shreecharan Kanchanavally
Graduate Theses and Dissertations
No abstract provided.
Uncloseting The Abused: A Historical Survey Of American Lesbian, Gay, Bisexual, And Transgendered Middle And Secondary Students' Struggle For Protection, Mary Lou Arons
Graduate Theses and Dissertations
No abstract provided.
The Plant And Mycorrhizal Fungal Community Dynamics Of A Desertified Region Of Semiarid Northern Chile, Sarah Healy Vigderson
The Plant And Mycorrhizal Fungal Community Dynamics Of A Desertified Region Of Semiarid Northern Chile, Sarah Healy Vigderson
Graduate Theses and Dissertations
No abstract provided.
Writing In The Biology Classroom, Kristen Ann Occulto
Writing In The Biology Classroom, Kristen Ann Occulto
Graduate Theses and Dissertations
No abstract provided.
Bacterial Activity And Biomass In Alkaline Fen Sediments: Seasonal Field And Laboratory Studies, Timothy C. Gsell
Bacterial Activity And Biomass In Alkaline Fen Sediments: Seasonal Field And Laboratory Studies, Timothy C. Gsell
Graduate Theses and Dissertations
No abstract provided.
Histos: The Development And Implementation Of A Biological Lab Program, Robert J. Guizzo
Histos: The Development And Implementation Of A Biological Lab Program, Robert J. Guizzo
Graduate Theses and Dissertations
This project addresses those heretofore limited experiments and laboratory activities associated with a secondary biology course having a core emphasis on human anatomy and physiology. Such a course has been offered at Oakwood High School for nearly two decades. It offers labs to students in two distinct, yet related areas. Anatomy labs provide students with a wide variety of dissectable materials. Whole specimens as well as individual organs are examined and analyzed. In association with lectures, demonstrations, and AV presentations, these labs present a series of unique and critical opportunities in the study of human macroscopic structure. Physiology labs center …
Natural And Dfmo-Induced Transformation Of Trypanosoma Brucei: A Comparison Of Several Biochemical Parameters, Scott Michael Hartman
Natural And Dfmo-Induced Transformation Of Trypanosoma Brucei: A Comparison Of Several Biochemical Parameters, Scott Michael Hartman
Graduate Theses and Dissertations
Morphological and biochemical transformation of bloodstream form trypanosomes from long slender (LS) to short stumpy (SS) forms is an important event for completion of the trypanosome life cycle because it pre-adapts the trypanosome for the change of environment upon transfer to the tsetse fly vector. The drug a-difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase, inhibits polyamine biosynthesis and has been found to induce the transformation from LS to SS forms. DFMO has been used routinely as an inducer of LS to SS transformation in molecular and biochemical experiments studying this transformation event. In this study we have compared natural …
A Design Of A Proposed Computer-Assisted General Biology Program, Diane M. Blose-Ivory
A Design Of A Proposed Computer-Assisted General Biology Program, Diane M. Blose-Ivory
Graduate Theses and Dissertations
No abstract provided.
Curriculum For An Extended Summer Field Studies Program, Charles E. Case
Curriculum For An Extended Summer Field Studies Program, Charles E. Case
Graduate Theses and Dissertations
No abstract provided.
Development Of A Handbook Of Demonstrations To Assist Instruction Of Biology In The High School Classroom, Kevin Craig Jones
Development Of A Handbook Of Demonstrations To Assist Instruction Of Biology In The High School Classroom, Kevin Craig Jones
Graduate Theses and Dissertations
No abstract provided.
A Right Hemispheric Approach To The Teaching Of Sixth Grade Life Science, Jacqueline Lee Fenstermaker Covert
A Right Hemispheric Approach To The Teaching Of Sixth Grade Life Science, Jacqueline Lee Fenstermaker Covert
Graduate Theses and Dissertations
No abstract provided.