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Molecular And Functional Characterization Of Bacterial Communities In Organic Farm And Pasture Soils, Sherie L. Edenborn
Molecular And Functional Characterization Of Bacterial Communities In Organic Farm And Pasture Soils, Sherie L. Edenborn
Graduate Theses, Dissertations, and Problem Reports (ETD)
Culture-dependent denaturing gradient gel electrophoresis (CD DGGE) fingerprinting of the 16S rRNA gene was developed to characterize mixed soil bacterial communities recovered on agar plates. CD DGGE analyses resulted in clear banding patterns of sufficient complexity (16--32 major bands) and reproducibility to investigate differences in bacterial communities in silt loam soils. Replicate CD DGGE profiles from plates inoculated with less-dilute samples (10 -3) had a higher band count and were more similar (72--77%) than profiles from more-dilute samples (51--61%). When CD DGGE fingerprints were compared to profiles constructed from 16S rRNA genes obtained from culture-independent clone libraries (CB DGGE profiles) …
An Interpolation Method For Stream Habitat Assessments With Reference To The Crystal Darter, Kenneth Richard Sheehan
An Interpolation Method For Stream Habitat Assessments With Reference To The Crystal Darter, Kenneth Richard Sheehan
Graduate Theses, Dissertations, and Problem Reports (ETD)
Crystallaria asprella spp. (Elk River crystal darter) is documented only in Elk River, West Virginia. Little life history detail is known about this lotic, benthic-dependent species. Previous studies have led to a very basic understanding of the use of depth, flow and substrate of the crystal darter (Crystallaria asprella), and less is known about the Elk River crystal darter. Due to substrate-specificity in crystal darters (which reportedly bury in sand), a study concerning benthic stream habitat modeling was undertaken. Substrate and depth modeling using >5% of an area sampled at a resolution of 0.093 m2 square spatial data in a …