Digital Commons Network^™

Genomic Variation, Adaptive Evolution, And Invasive And Evolutionary History Of Microstegium Vimineum, Cameron William Corbett

Graduate Theses, Dissertations, and Problem Reports (ETD)

Invasive species can cause environmental and economic damage, but can offer unique opportunities to study rapid evolution and adaptation in novel environments. Microstegium vimineum (stiltgrass) is a prolific invasive grass in the eastern United States. Historical herbaria collections were used to identify patterns of invasion history and population dynamics over a spatial and temporal scale. Analyses of plastid genomes reveal a complex invasion history, including a single introduction in the southern United States linked to Japan and multiple repeated introductions in the northern United States from various source populations. Spread of introduced populations provide evidence of admixture among previously geographically …

The Effect Of Sars-Cov-2 On Bacterial Diversity And Abundance In Community Wastewater, Joshua Lambert

Graduate Theses, Dissertations, and Problem Reports (ETD)

Severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) enters human respiratory cells through binding of its spike (S) protein to the ACE-2 receptor, which is highly expressed in lung cells, but is also found in over 150 other cell types encompassing major organs including GI enterocytes. SARS-CoV-2 causes gut dysbiosis in infected individuals and patients with COVID-19 and also coincides with lower alpha diversity of the individual’s gut microbiome. Certain gut microbiome-associated genera are lower in abundance in COVID-19 patients including Lachnospira, Prevetolla, Roseburia, Bifidobacterium, and Faecalibacterium while genera such as Bacteroides, Enterococcus and Proteobacteria are increased during a SARS-CoV-2 …

Machine Learning And Uass For Managing Autumn Olive (Elaeagnus Umbellata) On Reclaimed Surface Mines, Sean Michael Keane

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research introduces an innovative approach for managing autumn olive (Elaeagnus umbellata) on reclaimed surface mines through the integration of machine learning algorithms and unmanned aerial systems. The proliferation of autumn olive presents significant ecological challenges on disturbed landscapes, necessitating more efficient detection and control methods. This study evaluated Random Forest classification and deep learning approaches using the U-Net architecture for identifying autumn olive across four phenological stages using high-resolution imagery. The RGB U-Net model achieved F1-scores of 0.927, 0.912, and 0.871 for autumn olive detection during peak, late, and senescence stages respectively, outperforming both multispectral and vegetation …

Neural And Computational Approach To Understanding Environmental Modulation Of Behavioral Identity In Zebrafish, John W. Hageter

Graduate Theses, Dissertations, and Problem Reports (ETD)

Organisms rely on behavior for survival. Animals engage in behaviors that allow for feeding, mating, exploring and navigating their environment among others. Necessary for these behaviors to develop are the environmental factors and underlying circuitry which make behavior possible. Specifically, how the environment guides underlying neural circuitry to develop unique facets or phenotypes of a larger behavior are key to understanding why unique behaviors exist. In this thesis, I build foundational evidence for determining these mechanisms through the use of the zebrafish local search behavior. This is a behavior that zebrafish employ following the loss of environmental illumination where they …

A Multiscale Ai Framework For Forest And Agriculture Health Monitoring: Drone-Based Object Recognition And Segmentation For Automated Ecological Assessment, Sruthi Keerthi Valicharla

Graduate Theses, Dissertations, and Problem Reports (ETD)

Forest and agricultural ecosystems are increasingly at risk due to invasive species, pests, and diseases, necessitating scalable, automated, and intelligent monitoring solutions. Traditional field based forest and agriculture health assessments are limited by cost, time, and spatial coverage. This dissertation presents a multiscale deep learning framework that automates forest and agriculture health monitoring using drone imagery and computer vision techniques. The system operates across three spatial levels: forest level, tree level, and leaf level, combining object detection, segmentation, and classification models to support large scale ecological assessment.

At the forest level, high-altitude drone imagery is processed using object detection and …

Microbial And Plant Biodiversity In Appalachian Agroecosystems And Their Implications For Soil Health, Kinsey Reed Close

Graduate Theses, Dissertations, and Problem Reports (ETD)

The sustainability of agricultural landscapes depends on understanding the complex interactions between plants, soil, and microbes. In Appalachia, a region shaped by complex terrain and long histories of human and ecological interaction, small family farms produce hay, livestock, and row crops. These agroecosystems, though often treated as “artificial” by many ecologists, have the potential to support both food production and biodiversity conservation if managed with an understanding of ecological complexity. This dissertation examines how plant and microbial diversity interact with soil and management to influence nutrient cycling, soil health, and long-term resilience in Appalachian agricultural systems. A literature review revealed …

Effects Of Environmental And Anthropogenic Gradients On Fish Beta Diversity In Central Appalachian Streams, Alanna Kaylee Lowther Smith

Graduate Theses, Dissertations, and Problem Reports (ETD)

Understanding the environmental and spatial drivers of community composition is essential for conserving freshwater biodiversity. We examined how environmental gradients, land cover, and spatial structure influence fish beta diversity in wadeable streams across West Virginia, USA. We quantified total beta diversity and partitioned it into species replacement and richness difference components and used local contributions to beta diversity (LCBD) to identify compositionally unique sites. Community–environment relationships were evaluated with distance-based redundancy analysis (dbRDA), and a beta regression GLMM was used to test how elevation, slope, land cover, and stream order (factor) predicted LCBD while accounting for spatial structure (dbMEMs) and …

Examining The Role Of Microbial Communities In Soil Carbon Cycling Across Levels Of Complexity, Chansotheary Dang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Microorganisms provide the foundation for many ecosystem processes and are important drivers of carbon and nutrient cycling. The advancement of next generation sequencing has allowed researchers to lift open the lid on the ‘black box’ in microbial ecology. While previously unculturable microbes can now be characterized with the 16S rRNA gene, answering ‘who’s there?’ is only part of the challenge. Hidden inside the ‘black box’ remains the question, ‘who’s doing what?’. Recent development in trait-based methods like quantitative stable isotope probing (qSIP) equipped microbial ecologists with the tool to connect taxon-specific traits to ecosystem processes. Despite the growing body of …

Measures Of Feed Efficiency In Beef Cattle: Biological Basis And Effect On Response To Dietary Supplementation, Modoluwamu Idowu

Graduate Theses, Dissertations, and Problem Reports (ETD)

During the feedlot receiving period, newly weaned beef cattle face various stressors, including weaning, transportation, vaccination, and exposure to pathogens. Additionally, changes in diet and low dry matter intake contribute to nutrient deficiencies, heightening susceptibility to disease and performance declines. Therefore, we examined the effects of dietary supplementation with a multicomponent blend of prebiotics and probiotics on the health, immune status, metabolism, and performance of newly weaned beef steers during a 35-day receiving period. Our results showed that compared to the control group, the supplemental additive (SYNB) increased average daily gain (ADG), dry matter intake, and meal events during the …

Development And Implementation Of Occupational Therapy Group Protocols For Individuals In Recovery For Substance Use Disorder: Considering Social Determinants Of Health And Occupational Areas Of Need, Samantha Jane Windisch

Graduate Theses, Dissertations, and Problem Reports (ETD)

Substance use disorders (SUDs) are becoming a more widespread issue globally. According to the National Survey on Drug Use and Health, 19.7 million adults in the U.S. suffered from an SUD in 2017. (SAMSHA, 2018). Recurrent alcohol and/or drug use that results in significant disability, including health issues, and/or a failure to prioritize obligations like employment, school, or family life are characteristics of SUD (SAMHSA, 2022). Research suggests that healthcare providers must integrate cutting-edge and successful treatments for this population to lower the risk of overdose and relapse rates, given the swift global spread of addictive disorders. In the past, …

Long-Term Prescription Opioid Use, Cancer Stage, And Economic Burden Among Older Cancer Survivors With Non-Cancer Chronic Pain Conditions, Rudi Safarudin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Opioids are commonly prescribed to treat pain conditions, which are highly prevalent among older adults (≥65 years). Recent evidence shows that opioids may have carcinogenic effects through immunosuppression and free-radical pathways, which could promote cancer growth. Two population-based studies have reported an association of opioid use with incident cancer. Many individuals with non-cancer chronic pain conditions (NCPCs) may use opioids for a long term. However, the association of long-term prescription opioid (LPO) use with cancer stage at diagnosis is still unknown. Furthermore, evidence suggests that individuals with cancer and pre-existing NCPCs may persistently use opioids, even in the absence of …

Exploring The Usage Of Acid Mine Drainage Sludge As A Soil Amendment For Reclaimed Mine Lands, Brady Watters

Graduate Theses, Dissertations, and Problem Reports (ETD)

Acid mine drainage (AMD) is a form of water pollution generated when water and oxygen come into contact with sulfide minerals, forming metal ions, sulfuric acid, and sulphates in solution. AMD is characterized by its toxic metals content and low pH, both of which are an environmental concern. The treatment of AMD separates the contaminants into a high-water content sludge. This sludge is a pure waste product under current evaluation for alternative uses as well as improvements to storage and treatment processes. This thesis focused on analyzing the potential for AMD sludge to be used in recently reclaimed lands as …

Using Extended Two-Step Floating Catchment Area To Map Children’S Level Of Access To Physical Books In West Virginia, Heather A. Maxey

Graduate Theses, Dissertations, and Problem Reports (ETD)

The state of West Virginia lacked a comprehensive, in-depth analysis of the availability of physical books across the state. Access to reading material is vital to a child’s educational development as well as lifelong learning beyond childhood. To guide policy decisions and planning for outreach which addresses reading levels and beyond, I mapped levels of access stemming from socioeconomic barriers, spatial barriers, and an integrated index of both types of barriers. To map spatial access, I utilized a modified Extended Two-Step Floating Catchment Area model, deriving techniques from Hong et al’s 2023 article addressing access for health care in WV. …

Driving After Marijuana Use Among Us College Students During The Covid-19 Pandemic, Yuni Tang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: Adolescents and college-aged adults account for a large proportion of the United States population and are susceptible to drugs, including marijuana. Since the late 1960s in the United States, numerous states have legalized marijuana for medical and recreational purposes. These recent policy changes may alter people’s attitudes and behaviors about marijuana use, particularly young adults. The expansion of state-level marijuana legalization may also increase accessibility in younger populations and increase reckless driving behavior associated with marijuana use, including driving after marijuana use. During the COVID-19 pandemic, adolescents and young adults experienced heightened stress and anxiety, which have significantly contributed …

Application Of Computational Biophysics Techniques To Characterize Cell Membrane-Associated Events, Kyle Billings

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cell membranes are crowded environments which can modulate protein structure-function relationships through interaction with lipids, other proteins, carbohydrate structures and so on. This work focuses the impact of the membrane environment on two varieties of peptides: Microbial rhodopsin proteins, and cyclic peptides.

Life on Earth is dependent on the ability of plants and microbes to harness sunlight for energy production. Their ability to transform light into carbohydrates requires tailor-made machinery, and for a wealth of microorganisms, microbial rhodopsin proteins (MR) are critical for maintaining the concentration gradients used to produce the energy molecule Adenosine triphosphate (ATP). The central retinal molecule …

Understanding Visitor Use At Florida Keys National Marine Sanctuary, Melissa Maria Hurtado Alvarez

Graduate Theses, Dissertations, and Problem Reports (ETD)

Effective management and monitoring of visitor recreational use is fundamental in marine protected areas. It is common to hear that tourism and outdoor recreation are often qualified as double-edged activities, making valuable socioeconomic contributions but also causing degradation of the environment. The convergence of social and natural resource research and practices can help managers to create better policies that will maximize human benefits from, and minimize human pressures on, ocean and coastal environments. For this study online surveys were sent via Qualtrics to email addresses obtained from the state of Florida during the summer of 2020. The first paper assessed …

Environmental Controls On The Terrestrial Water Cycle In Forested Mountain Ecosystems., Luis Andrés Guillén

Graduate Theses, Dissertations, and Problem Reports (ETD)

Water is a key resource to natural ecosystems and human societies alike, and the water cycle is fundamentally linked to the climate and the characteristics of catchments. However, the challenges posed by environmental change makes it imperative to understand how the water cycle is affected by biotic and abiotic factors, in particular, in areas that are crucial sources of water like forested headwater catchments. Therefore, this doctoral dissertation aims to advance the knowledge on the dynamics between climate, vegetation and landscape that determine the water balance of forested mountain ecosystems. This document presents five chapters, an introductory chapter, three standalone …

Compound-Specific Isotope Analysis Of Amino Acids In Biological Tissues: Applications In Forensic Entomology, Food Authentication And Soft-Biometrics In Humans, Mayara Patricia Viana De Matos

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this work we demonstrate the power of compound-specific isotope analysis (CSIA) to analyze proteinaceous biological materials in three distinct forensic applications, including: 1) linking necrophagous blow flies in different life stages to their primary carrion diet; 2) identifying the harvesting area of oysters for food authentication purposes; and 3) the ability to predict biometric traits about humans from their hair.

In the first application, we measured the amino-acid-level fractionation that occurs at each major life stage of Calliphora vicina (Robineau-Desvoidy) (Diptera: Calliphoridae) blow flies. Adult blow flies oviposited on raw pork muscle, beef muscle, or chicken liver. Larvae, pupae …

Historic Log Buildings As Archives Of Past Forest Ecology, Kristen K. De Graauw

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation is composed of three separate but related manuscripts with the common theme of using historic log buildings from the central Appalachian Mountain region of eastern North America as ecological archives. In Chapter 1, I explore the biases, limitations, and ecological applications of tree-ring data from historic log buildings. European immigrants selected trees from a forested stand based on species, log sizes, and construction locations. Despite this selection bias, ecological information can be gleaned from historic log buildings, which offer a complementary record of past forest ecology and represent a site type that is not often associated with old-growth …

Differentiating Human Populations Based On K-Mer Classification Of Hand Bacteria, Thrisha Doppala

Graduate Theses, Dissertations, and Problem Reports (ETD)

Bacterial communities found in and on the human body are not only used in studying human health conditions but are also effective in differentiating individuals due to their distinct profiles. Human palm regions harbor relatively more diverse bacterial communities and are indicative of population groups, life styles, geographic locations, age groups and health conditions. Sequences extracted from hypervariable region V3 of the 16S rRNA bacterial gene of hand bacterial samples from 9 different population groups were classified into Operational Taxonomic Units (OTU) with GreenGenes reference taxonomy using RDP (Ribosomal Database Project) classifier. Frequencies of identified OTUs were used to study …

Examination And Characterization Of Brook Trout Ecology Before And After A Broad Scale Disturbance Event In Headwater Streams Of West Virginia, Usa, Ross Gilbert Andrew

Graduate Theses, Dissertations, and Problem Reports (ETD)

No abstract provided.

Towards A Better Understanding Of Paleoindian Native American Settlement In Southern Ohio: A Multi-Scalar Approach, Matthew P Purtill

Graduate Theses, Dissertations, and Problem Reports (ETD)

No abstract provided.

Explanatory And Causality Analysis In Software Engineering, Yasser Ali Alshehri

Graduate Theses, Dissertations, and Problem Reports (ETD)

Software fault proneness and software development efforts are two key areas of software engineering. Improving them will significantly reduce the cost and promote good planning and practice in developing and managing software projects. Traditionally, studies of software fault proneness and software development efforts were focused on analysis and prediction, which can help to answer questions like `when’ and `where’. The focus of this dissertation is on explanatory and causality studies that address questions like `why’ and `how’.

First, we applied a case-control study to explain software fault proneness. We found that Bugfixes (Prerelease bugs), Developers, Code Churn, and Age of …

Behavior Of Stiff-Tailed Diving Ducks And West Indian Whistling Ducks In Laguna Cartagena National Wildlife Refuge, Puerto Rico, Nick S. Goodman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Masked ducks (Nomonyx dominicus), migratory northern ruddy ducks (Oxyura jamaicensis jamaicensis), non-migratory West Indian ruddy ducks (Oxyura j. jamaicensis Gmelin), and West Indian whistling-ducks (Dendrocygna arborea) are all duck taxa that have declining populations in Puerto Rico. Masked ducks are listed as endangered in Puerto Rico and are a species of concern in the Caribbean while West Indian whistling ducks are considered an "at-risk" species listed as vulnerable. All 4 taxa of duck have areas of their life history where information is lacking including behavioral information. I studied the 4 taxa of duck at Laguna Cartagena National Wildlife Refuge from …

The Dynamics Of Economic Status And Health Among Working-Age Adults In The United States, Abdulkarim Mohammad Meraya

Graduate Theses, Dissertations, and Problem Reports (ETD)

The relationship between economic status and health has been documented in the previous literature. Economic, sociological and epidemiological studies have indicated that higher economic status is associated with better physical and mental health. While these studies have made significant contributions in this area, a comprehensive evaluation of the relationships between economic indicators and health is still lacking. Most studies focused on a single measure of economic status or health. Further, most studies have focused on one-direction -- economic indicators affecting health; little is known about the effect of health improvement on gain in economic status. Furthermore, only a few studies …

Wildlife In An Anthropogenically-Driven World: How Humans Have Shaped The Distribution, Genetic Composition, And Gene Expression Of North American Forest Hawks (Genus: Accipiter), Meghan K. Jensen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Humans are causing drastic environmental change on a global scale and this trend strongly influences the evolution of species. It is also becoming clear that tolerances to anthropogenic disturbance varies widely among organisms. Therefore, understanding the mechanisms by which wildlife cope with humans is a pressing question in modern ecology. North America's forest raptors (Genus: Accipiter) are a useful model for investigating the effects of humans on wildlife species. All three Accipiter species experienced historic demographic declines as a result of anthropogenic activities, yet each species has rebounded differently since these declines. One species in particular is now exploiting urban …

The Utility Of Fine-Scale Remote Sensing Data For Modeling Habitat Characteristics And Breeding Bird Species Distributions In An Appalachian Mature Deciduous Forest., James Sheehan

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this study, I tested the potential for remote sensing data with a high spatial resolution to model breeding forest bird species and their habitat at a fine spatial scale. The research took place on ridgetops in a large, relatively contiguous Appalachian mature deciduous forest in northwestern WV, USA. The remote sensing data sources were a leaf-on QuickBird satellite image (0.6-m panchromatic and 2.4-m multispectral) and a 3-m digital elevation model (DEM). For the first part of the study, I extracted spectral and textural measures from the satellite image and terrain information from the DEM. I then used these data …

Using A Species Distribution Model And Site-Specific Microclimatic Variables To Model Presence Of Spodic Soil Properties And Relative Occurrence Rate Of Picea Rubens (Red Spruce) To Inform Red Spruce Management, Adrienne Nottingham

Graduate Theses, Dissertations, and Problem Reports (ETD)

Red spruce restoration efforts in the central Appalachians are of interest to land managers because of the reduced current extent and the numerous ecosystem services provided by red spruce forests. These land managers require information regarding the best places to focus restoration efforts. Studies attempting to locate optimal locations for red spruce restoration have been conducted to in the central Appalachians, most of which utilize modeling. In particular, podzolization, a soil formation pathway present under conifer vegetation in the central Appalachians has been used to help select areas for red spruce restoration. The effectiveness of using recent podzolization (as evidenced …

Space Use And Habitat Associations Of Long-Distance Migratory First-Year Golden Eagles (Aquila Chrysaetos) From Interior Alaska In A Changing Landscape, Mark D. Paulson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Understanding a species space use and habitat associations is integral to comprehensive wildlife management. Habitat associations change spatially and temporally and those changes may be especially dramatic for animals that cover long distances throughout their annual cycle. While many studies of habitat associations and space use concentrate on breeding season behavior, studies of migratory connectivity demonstrate how condition of habitats on non-breeding ranges potentially affect key demographic parameters, such as survival, reproduction, and movement in other seasons. This is also important because wildlife habitats, especially land cover, are changing rapidly from both anthropogenic and natural forces in direct and indirect …

Migratory Genomics Of Lake Sturgeon, Justine Whitaker

Graduate Theses, Dissertations, and Problem Reports (ETD)

Lake sturgeon are large, freshwater fish and the only species of sturgeon native to the Great Lakes. Their historic range extends from the Hudson Bay, down through the Great Lakes and into the Mississippi River basin. In the early 1900's, sturgeon became a valuable fishery and were soon overfished for both meat and caviar. This, in combination with damming, pollution, and habitat degradation, caused a drastic decline in their population. Some populations were extirpated, while others have been reduced to 1% of their historic population size (Auer, 1996). The St. Clair-Detroit River System (SCDRS) hosts one of the largest lake …