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Impact Of Male Age On The Outcome Of Assisted Reproductive Technology Cycles Using Donor Oocytes, Eyup Duran, D. Dowling-Lacey, S. Bocca, L. Stadtmauer, S. Oehninger

Eyup Hakan Duran

This study assessed the influence of the age of the male partner on the outcome of oocyte donation cycles. A total of 408 couples participating in 519 consecutive anonymous oocyte donation cycles were examined. Main outcome measures were fertilization rate, embryo quality, clinical pregnancy, implantation, miscarriage and live birth rates, as well as the total reproductive potential, which estimates the outcome from fresh and cryopreserved-thawed embryo transfers. A total of 241 cycles resulted in clinical pregnancy (48.5% of transfers). The mean embryo score for transferred embryos (ESTE) was higher in cycles resulting in pregnancy (P=0.003). Semen volume (P<0.001), sperm …

Sperm Dna Quality Predicts Intrauterine Insemination Outcome: A Prospective Cohort Study, Eyup Duran, M. Morshedi, S. Taylor, S. Oehninger

Eyup Hakan Duran

BACKGROUND: We aimed to investigate whether sperm DNA quality may predict intrauterine insemination (IUI) outcome. METHODS: The study was designed in a prospective cohort fashion, at a tertiary centre for reproductive medicine. A total of 119 patients underwent 154 cycles of IUI. Parameters related to demography, cycle management and semen sample used for IUI were evaluated. Conventional semen parameters, morphology (strict criteria), sperm DNA fragmentation and stability [evaluated by terminal deoxynucleotidyl transferase-mediated dUDP nick-end labelling (TUNEL) and acridine orange staining under both acid and acid + heat denaturing conditions respectively] were measured. The main outcome measure was clinical pregnancy, defined …

Efficacy And Pregnancy Outcome Of Two Methods Of Semen Preparation For Intrauterine Insemination: A Prospective Randomized Study., Mahmood Morshedi, E. Duran, Steven Taylor, Sergio Oehninger

Eyup Hakan Duran

OBJECTIVE: To examine pregnancy outcome with two methods of semen preparation for intrauterine insemination (IUI).

DESIGN: Prospective and randomized study.

SETTINGS: Academic tertiary center.

PATIENT(S): Three hundred eleven couples undergoing 676 consecutive cycles of assisted conception using IUI.

INTERVENTION(S): Semen samples collected for IUI were randomized to wash only or density gradient centrifugation (DGC) processing.

MAIN OUTCOME MEASURE(S): Various prepreparation and postpreparation semen parameters were used for IUI. The influence of the method of semen processing, and impact of various semen parameters and female factors on pregnancy were examined by receiver operating characteristics (ROC) curves, logistic regression, and life table …

Achievement Of Pregnancy In Globozoospermia With Y Chromosome Microdeletion After Icsi., Hulusi Zeyneloglu, Volkan Baltaci, E. Duran, Esra Erdemli, Sertac Batioglu

Eyup Hakan Duran

Pregnancy achieved with sperm from a patient with globozoospermia is rare, even after ICSI, since the activation of the oocyte may not occur in this disorder. Therefore, activation of the oocytes by piezoelectricity or calcium ionophores has been suggested, although spontaneous activation of the oocyte after ICSI has been reported in some cases. We report a successful pregnancy in a couple in which the male partner had globozoospermia with microdeletions in the Y chromosome with no further assisted activation after ICSI. During the diagnostic study of the husband, increased numerical chromosome abnormalities after fluorescent in-situ hybridization (FISH) and microdeletions in …

The Effect Of Age On The Expression Of Apoptosis Biomarkers In Human Spermatozoa, A. Colin, G. Barroso, N. Gomez-Lopez, Eyup Duran, S. Oehninger

Eyup Hakan Duran

OBJECTIVE: To evaluate the impact of age on the expression of apoptotic biomarkers in human spermatozoa. DESIGN: Cross sectional, prospective study. SETTING: Academic centers. PATIENT(S): Healthy volunteers with proven fertility, stratified by age (n = 25, range: 20-68 years). INTERVENTION(S): Examination of serum hormone levels and basic semen parameters, and assessment of early (plasma membrane translocation of phosphatidylserine) and late (DNA fragmentation) sperm apoptotic markers by flow cytometry (using Annexin-V binding and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling). MAIN OUTCOME MEASURE(S): Apoptosis markers. RESULT(S): Advancing male age was significantly and positively correlated with Annexin-V binding results. Although not significant, …

Zona Pellucida-Induced Acrosome Reaction In Human Sperm: Dependency On Activation Of Pertussis Toxin-Sensitive G(I) Protein And Extracellular Calcium, And Priming Effect Of Progesterone And Follicular Fluid., Alessandro Schuffner, Hadley Bastiaan, E. Duran, Zin-Yong Lin, Mahmood Morshedi, Daniel Franken, Sergio Oehninger

Eyup Hakan Duran

In these studies, we aimed to characterize the effects of the physiological, homologous agonists of the acrosome reaction, i.e. the zona pellucida (ZP) and progesterone/follicular fluid, on human sperm. The specific aims of our studies were: (i) to examine the dependency of the solubilized ZP-induced acrosome reaction on G(i) protein activation and presence of extracellular calcium; and (ii) to determine whether progesterone/follicular fluid exert a priming or synergist effect on the solubilized ZP-induced acrosome reaction. Highly motile sperm from fertile donors were exposed to the agonists in a microassay and the acrosomal status of live sperm was determined by indirect …

Predictive Value Of The Hemizona Assay For Pregnancy Outcome In Patients Undergoing Controlled Ovarian Hyperstimulation With Intrauterine Insemination., Murat Arslan, Mahmood Morshedi, Ebru Arslan, Steve Taylor, Arzu Kanik, E. Duran, Sergio Oehninger

Eyup Hakan Duran

OBJECTIVE: The hemizona assay (HZA) is an established functional test that examines in vitro sperm-zona pellucida binding capacity with high predictive power for fertilization outcome in IVF. The objective of this study was to evaluate the value of the HZA as a predictor of pregnancy in patients undergoing controlled ovarian hyperstimulation (COH) and intrauterine insemination (IUI). DESIGN: Prospective clinical study. SETTING: Academic center. PATIENT(S): Eighty-two couples with unexplained or male factor infertility that underwent 313 IUI cycles. INTERVENTION(S): Basic semen analysis and HZA were performed within three months of starting COH/IUI therapy. MAIN OUTCOME MEASURE(S): Hemizona index (HZI) and clinical …

Comparison Of Hyperbaric Oxygen And Medical Ozone Therapies In A Rat Model Of Experimental Distal Colitis, O. Altinel, S. Demirbas, E. Cakir, H. Yaman, I. Ozerhan, Eyup Duran, T. Cayci, E. Akgul, N. Ersoz, B. Uysal, B. Kurt, M. Yasar, S. Oter, Y. Peker

Eyup Hakan Duran

No abstract provided.

Intrauterine Insemination: A Systematic Review On Determinants Of Success., E. Duran, Mahmood Morshedi, Thinus Kruger, Sergio Oehninger

Eyup Hakan Duran

Intrauterine insemination (IUI) is a frequently indicated therapeutic modality in infertility. Here, a systematic review of the literature was performed to examine the current status of clinical and laboratory methodologies used in IUI and the impact of female and male factors on pregnancy success. Emphasis was centred in questioning the following: (i) the value of IUI against timed intercourse; (ii) IUI application with or without controlled ovarian hyperstimulation; (iii) timing and frequency of IUI; and (iv) impact of various parameters (male/female) on the prediction of pregnancy outcome. The odds of multiple pregnancy occurrence and its risk factors, as well as …

A Logistic Regression Model Including Dna Status And Morphology Of Spermatozoa For Prediction Of Fertilization In Vitro, Eyup Duran, T. Gurgan, S. Gunalp, M. Enginsu, H. Yarali, A. Ayhan

Eyup Hakan Duran

To determine predictive values of routine semen analysis, sperm morphology evaluation using strict criteria and DNA status for in-vitro fertilization (IVF), 66 consecutive couples undergoing IVF in a university hospital IVF programme were prospectively investigated. Semen samples from 66 men were evaluated by routine semen analysis, morphology evaluation using strict criteria and acridine orange staining for determination of DNA status. A new technique is described for acridine orange scoring which consisted of evaluation of two smears per case, with and without heat treatment. Resistance to heat-provoked denaturation was determined by the difference between two evaluations. A logistic regression model was …

Kernicterus By Glucose-6-Phosphate Dehydrogenase Deficiency: A Case Report And Review Of The Literature, G. Gurrola, J. Araúz, Eyup Durán, M. Auilar-Medina, R. Ramos-Payán, N. García-Magallanes, G. Pacheco, E. Meraz

Eyup Hakan Duran

No abstract provided.

Incidence Of Anti-Heparin/Platelet Factor 4 Antibodies And Heparin-Induced Thrombocytopenia In Medical Patients [Turkish], M. Demir, E. Tekgündüz, M. Edis, Eyup Duran, T. Kürüm

Eyup Hakan Duran

OBJECTIVE: Heparin-induced thrombocytopenia (HIT) is a life threatening complication of heparin therapy, causing thrombosis. The aim of our study was to find out the frequencies of HIT antibody seroconversion and clinical HIT in Turkish medical patients on different forms of heparins. METHODS: Our study included 61 patients who were on unfractionated heparin (UFH) (n: 37) and low molecular weight heparin (LMWH) (n: 24) therapies. The frequency of HIT antibody formation was determined by means of antigenic (ELISA), and functional assays (serotonin release assay-SRA). RESULTS: The seroconversion rates in UFH and LMWH groups were found to be 18.9% and 4.1% (ELISA), …

Presence And Significance Of Somatic Cell Apoptosis Markers In Human Ejaculated Spermatozoa., Sergio Oehninger, Mahmood Morshedi, S-L Weng, Steve Taylor, E. Duran, Stephen Beebe

Eyup Hakan Duran

Ejaculated spermatozoa, particularly in infertile men, have been shown to display morphological and biochemical features that are typical of an apoptotic phenotype in somatic cells. Deregulation of apoptosis is known to play roles in a number of disease processes, but roles for apoptosis in ejaculated spermatozoa and male infertility are poorly defined or have not been studied. Preliminary data demonstrated that populations of ejaculated spermatozoa express: (i) various degrees of plasma membrane translocation of phosphatidylserine and DNA fragmentation; and (ii) active caspase-3, the main executor of apoptosis in somatic cells, with an apparent exclusive cellular location to the mid-piece. Tests …

Somatic Cell Apoptosis Markers And Pathways In Human Ejaculated Sperm: Potential Utility As Indicators Of Sperm Quality, S. Taylor, S. Weng, P. Fox, Eyup Duran, M. Morshedi, S. Oehninger, S. Beebe

Eyup Hakan Duran

In this study we extended earlier work to determine whether sperm respond to somatic cell apoptotic stimuli and whether apoptotic phenotypes are significant indicators of human sperm quality. We evaluated ejaculated sperm from fertile donors and subfertile patients following purification of fractions of high and low motility. In unstimulated conditions, caspase enzymatic activity was higher in motile fractions from subfertile patients than in donors, and was higher in low motility fractions from both groups. Staurosporine, but not a Fas ligand or H2O2, significantly increased caspase activity, but only in high motility fractions. Procaspase-3, -7 and -9 and low levels of …