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Neurophysiology Of Spared Motor Tracts In Spinal Cord Injury, Dalton Louis Wolfe
Neurophysiology Of Spared Motor Tracts In Spinal Cord Injury, Dalton Louis Wolfe
Digitized Theses
Recent experimental and therapeutic initiatives have been directed towards enhancing the survival and function of preserved central axons following spinal cord injury (SCI). The continued development of these initiatives depends largely on the sensitivity of techniques to detect the presence of residual innervation in descending motor tracts. Detection of preserved innervation in SCI patients provided the focus of the present thesis.;Preserved motor innervation was investigated in patients with established SCI using transcranial magnetic stimulation of the motor cortex to elicit motor evoked potentials (MEPs) in muscles innervated below the level of the lesion. In particular, a set of experiments was …
Role Of Nitric Oxide In Interleukin-2 Therapy-Induced Capillary Leak Syndrome, Amila Orucevic
Role Of Nitric Oxide In Interleukin-2 Therapy-Induced Capillary Leak Syndrome, Amila Orucevic
Digitized Theses
Systemic interleukin-2 (IL-2) therapy has been limited by a major side effect known as "capillary leak syndrome", characterized by retention of extravascular fluid, severe hypotension, and multiple organ system dysfunction. The present study tested the hypothesis that an overproduction of nitric oxide (NO) induced by IL-2 therapy was, at least in part, responsible for this syndrome. Studies were undertaken in healthy or C3-L5 mammary adenocarcinoma bearing C3H/HeJ mice to evaluate whether treatment with inhibitors of NO synthesis (N{dollar}\rm\sp{lcub}G{rcub}{dollar}-Methyl-L-Arginine, NMMA and N{dollar}\rm\sp{lcub}G{rcub}{dollar}-Nitro-L-Arginine methyl ester, L-NAME) can prevent IL-2 induced capillary leakage without compromising the therapeutic benefit of IL-2. Intraperitoneal IL-2 therapy …
P53-Dependent Tumorigenesis And Gene Expression In Mammalian Brain, Meyel Donald Van
P53-Dependent Tumorigenesis And Gene Expression In Mammalian Brain, Meyel Donald Van
Digitized Theses
Astrocytic gliomas are the most common brain cancers of adults. The goal of this research was to examine the molecular genetic determinants of gliomas, with particular emphasis on the tumor suppressor gene P53. First, I studied the frequency, nature and timing of P53 mutations in human gliomas. I observed that glioma-prone families do not have inherited mutations of P53, nor of the Nf1-GRD, Nf2, MTS1, MTS2, or CDK4 genes. However, a high frequency of loss of heterozygosity at chromosome 9p was noted in gliomas that cluster in families. In a second study of low-grade gliomas that progressed to higher grade …
Central Cardiorespiratory And Peripheral Metabolic Influences On Kinetics Of Oxygen Uptake In Older Humans, Philip David Chilibeck
Central Cardiorespiratory And Peripheral Metabolic Influences On Kinetics Of Oxygen Uptake In Older Humans, Philip David Chilibeck
Digitized Theses
The purpose of this thesis was to determine which factors influence oxygen uptake (VO{dollar}\sb2){dollar} kinetics during exercise in old individuals. These factors may include central {dollar}\rm O\sb2{dollar} delivery (i.e. cardiac output kinetics), the rate of O{dollar}\sb2{dollar} delivery at the exercising muscle, and the rate at which mitochondria can use O{dollar}\sb2.{dollar} Fitness level may also influence VO{dollar}\sb2{dollar} kinetics, by affecting the above factors. These factors were estimated by measuring heart rate (HR) kinetics, muscle capillarization, and citrate synthase (CS) activity, respectively, in groups of old ({dollar}\sim{dollar}65y) and young ({dollar}\sim{dollar}26y) individuals. Capillarization and CS activity of the lateral gastrocnemius were compared to …
Examination Of Nucleoside Transport In The Mammalian Nervous System, Kenneth William Jones
Examination Of Nucleoside Transport In The Mammalian Nervous System, Kenneth William Jones
Digitized Theses
In rabbit and guinea pig cortical synaptosomes, the nucleoside transport inhibitors nitrobenzylthioinosine and dipyridamole were used to test the hypotheses that the nitrobenzylthioinosine-sensitive and -resistant ({dollar}\sp3{dollar}H) dipyridamole binding sites are associated with the es and ei nucleoside transporters, respectively. In addition, the hypothesis that the es and ei nucleoside transporters in rabbit cortical synaptosomes differ in their selectivity for compounds besides nitrobenzylthioinosine was tested, Nitrobenzylthioinosine-sensitive ({dollar}\sp3{dollar}H) dipyridamole binding and ({dollar}\sp3{dollar}H) nitrobenzylthioinosine binding involved the same site on the es transporter. The relative proportions and inhibitor sensitivities of nitrobenzylthioinosine-resistant ({dollar}\sp3{dollar}H) dipyridamole binding could not be correlated with nitrobenzylthioinosine-resistant nucleoside transport. This, …
Structural Basis For Human Anti-Dna Antibody Specificity, Mahmoud Mahmoudi
Structural Basis For Human Anti-Dna Antibody Specificity, Mahmoud Mahmoudi
Digitized Theses
Anti-DNA antibodies are hallmarks of SLE both in human and inbred lupus prone mice. The role of these antibodies in disease pathogenesis, particularly in glomerulonephritis has been documented. It is now well accepted that the genetic potential to generate anti-DNA antibodies also exists in the normal B cell immune repertoire. This genetic potential has been revealed by detection of anti-DNA antibodies in serum of normal individuals and by immortalizing DNA reactive B cells as hybridomas and as transformed cell lines. There is growing evidence that anti-DNA antibodies from the normal B cell repertoire and disease-associated anti-DNA antibodies share V region …
Prenatal Risk Factors For Neurological Abnormalities In The Term And Near Term Neonate, Kathleen Owen O'Connor
Prenatal Risk Factors For Neurological Abnormalities In The Term And Near Term Neonate, Kathleen Owen O'Connor
Digitized Theses
An abnormal neurological examination in the neonatal hospital stay may indicate a transient or permanent neurological disturbance. Abnormal neurological symptoms therefore place an infant at increased risk for subsequent neurological problems.;This case-control study was designed to investigate prenatal and perinatal risk factors for an abnormal neurological examination in the term infant. Infants were born between 1 January, 1988 and 30 June, 1990. The sample consisted of 222 neonates with neurological abnormalities and 666 controls who were free of such problems. Data on the prenatal histories of their mothers, course of the pregnancy, intrapartum events and status of the infant after …
Evaluation Of Doppler Ultrasound Velocity And Flow Measurements, Daniel William Rickey
Evaluation Of Doppler Ultrasound Velocity And Flow Measurements, Daniel William Rickey
Digitized Theses
Doppler ultrasound instruments have been used clinically for about 30 years and have proved to be highly reliable for the detection of arterial disease. In addition, a number of different Doppler instrument designs and techniques have been proposed to provide quantitative measurements such as volume blood flow, percent stenosis, and waveform indices. Before any measurement technique can be used, it must be validated.;We chose a two-pronged approach to the problem of evaluating Doppler ultrasound measurements of blood flow. We first developed a flow system that mimics the vasculature. The flow system comprises a computer-controlled pump, blood mimic, and a wall-less …
Dietary Intake And Health Status Of Immigrants And Non-Immigrants In Ontario And Quebec, Joceline Pomerleau
Dietary Intake And Health Status Of Immigrants And Non-Immigrants In Ontario And Quebec, Joceline Pomerleau
Digitized Theses
Introduction: Dietary intake and health status of immigrants to various countries are often different from those of non-immigrants living in these countries. Because the diversity of the immigrants and ethnic minorities in Canada is increasing, the association of place of birth with nutrition and health among Canadians needs to be investigated.;Methods: Cross-sectional data from the 1990 Ontario Health Survey (OHS) and the 1990 Quebec Heart Health and Nutrition Survey (QHHNS) were used. A selection of nutritional (dietary, anthropometric, knowledge variables) and health (health problems, lipidemia, consultations with health professionals, cut-down/bed-days, self-perceived health) outcomes were examined among adult respondents (OHS: n …
Design Of A Collimated Line Source For Transmission Ct Imaging Of The Head With A Spect System, Brad James Kemp
Design Of A Collimated Line Source For Transmission Ct Imaging Of The Head With A Spect System, Brad James Kemp
Digitized Theses
Single photon emission computed tomography (SPECT) has the potential to provide absolute quantitative information about the function of an organ. However, two factors which limit accurate quantification are the attenuation of the gamma rays emitted from the radiopharmaceutical concentration and the inability to define the anatomic region being quantified. Presented in this thesis is an attenuation correction that compensates SPECT brain images for attenuation and a transmission imaging system that (a) provides the distribution of attenuation coefficients required for the attenuation compensation and (b) simplifies regional identification by registering the functional SPECT images onto the anatomic transmission images. The attenuation …
Postsynaptic Cholinergic Control Of Cardiac Function In Aging, Na Su
Postsynaptic Cholinergic Control Of Cardiac Function In Aging, Na Su
Digitized Theses
The present study using isolated constant flow-perfused heart from adult (8 month-old) and aged (26 month-old) Fischer 344 rats for the first time demonstrated a striking enhancement of the negative chronotropic, inotropic and coronary vasoconstriction responses of the aging heart to postsynaptic cholinergic stimulation.;The approaches used and the results demonstrated were the following. (i) Acetylcholinesterase activity measured by the method of Ellman et al (1961) declined significantly in the atria and ventricles of the aged compared to adult rats. (ii) No age-related difference was seen in muscarinic receptor number in the atria and ventricles as assessed by {dollar}\lbrack\sp3\rm H\rbrack QNB{dollar} …
Role Of Protein Kinase C In Mediating The Effects Of Physiological And Pathophysiological Interventions In The Heart, Christopher Andrew Ward
Role Of Protein Kinase C In Mediating The Effects Of Physiological And Pathophysiological Interventions In The Heart, Christopher Andrew Ward
Digitized Theses
Protein kinase C has been implicated as a mediator of myocardial function following both physiological and pathophysiological stimuli. The purpose of the present study was to examine the functional effects of PKC in mediating phorbol esters as well as receptor-induced effects in myocardial tissues. Additionally, the possible role of PKC in mediating cardiac pathophysiology was assessed under conditions which mimicked aspects of myocardial ischemia/reperfusion. The effects of phorbol esters on cardiac function were assessed in isolated perfused guinea pig hearts. The effects of phorbol esters, acidosis/realkalinization and hydrogen peroxide were assessed in isolated guinea pig ventricular myocytes to determine PKC-dependent …
Automated Analysis Of Nuclear Medicine Images: Towards Artificial Intelligence Systems, Piotr Jan Slomka
Automated Analysis Of Nuclear Medicine Images: Towards Artificial Intelligence Systems, Piotr Jan Slomka
Digitized Theses
Automated methods for the analysis of nuclear medicine images could provide an objective diagnosis, and means to transfer sophisticated expertise to less experienced centres. The goal of this study was to develop software methods for the automated analysis of (a) Quality Control (QC) images, and (b) myocardial perfusion tomography images.;The system for the automated analysis of QC images was based on feature extraction algorithms, which provided input to a higher level diagnostic expert system. Several features characterizing QC images were defined. Rule-based and object-oriented expert systems were created to guide personnel in QC procedures, detect gamma camera faults, and suggest …
Modulation Of Experimental Allergic Encephalomyelitis In The Sjl/J Mice By Cytokines And Anti-Cytokine Antibodies, Trang Thi Duong
Modulation Of Experimental Allergic Encephalomyelitis In The Sjl/J Mice By Cytokines And Anti-Cytokine Antibodies, Trang Thi Duong
Digitized Theses
Immunization with myelin basic protein in complete Freund's adjuvant resulted in only mild chronic-relapsing experimental allergic encephalomyelitis (EAE) with very low incidence in SJL/J mice, and no disease at all in A/J, BALB/c, C3H/HeJ, DBA/2, AKR and NZW mice. However, treatment of mice with anti-IFN{dollar}\gamma{dollar} (aIFN) monoclonal antibody (mAb) at the time of immunization determined severe disease in all those strains except AKR. Similarly, the passive induction of EAE in the SJL/J mice and in two of three resistant strains examined was significantly enhanced by aIFN mAb administration. In the SJL/J mice, administration of anti-interleukin 2 (aIL2) mAb had only …
Using Model Systems To Study Rat Lens Membrane Damage During Cortical Cataract Formation, Fusun Kilic
Using Model Systems To Study Rat Lens Membrane Damage During Cortical Cataract Formation, Fusun Kilic
Digitized Theses
Cataract formation is a complex, multifactorial and multistep process: it is associated with cellular and biochemical changes in the lens. In the present work, steps in the pathway to cataract development were followed in vitro using two different reagents which induce cataract formation by two different mechanisms: elevated glucose (55.6 mM), as a model for in vivo diabetic cataractogenesis and an actin monomer-stabilizer, cytochalasin D (10{dollar}\sp{lcub}-5{rcub}{dollar} M, CD). Findings were utilized to classify and arrange the events in a common pathway and to focus on the mechanism of cataract production.;The development of cortical subcapsular opacity in cultured lenses was elucidated …
Characterization Of Intestinal Epithelial Cells In Acute Intestinal Allograft Rejection, Xian-Chang Li
Characterization Of Intestinal Epithelial Cells In Acute Intestinal Allograft Rejection, Xian-Chang Li
Digitized Theses
Intestinal transplantation (IT) remains highly experimental compared to other solid organ transplants because of poorly defined mechanisms of graft rejection. In this project, the role of luminal bacteria-derived endotoxin and inflammatory cytokines (IL-1, IL-6, TNF-{dollar}\alpha){dollar} in intestinal epithelial damage during acute graft rejection was examined. In a novel mouse model of IT, the acute intestinal rejection was associated with elevated levels of endotoxin, IL-6, and TNF-{dollar}\alpha{dollar} in the peripheral blood. The progressive increase in endotoxin and TNF-{dollar}\alpha{dollar} levels correlated well with the histologic severity of epithelial damage. To further define the role of endotoxin and inflammatory cytokines on intestinal epithelial …
Regulation Of Antibody Production And Its Dysregulation In Autoimmunity, Colin Charles Anderson
Regulation Of Antibody Production And Its Dysregulation In Autoimmunity, Colin Charles Anderson
Digitized Theses
B cells can be stimulated to produce antibody by antigens or by polyclonal activators such as bacterial lipopolysaccharide (LPS). Mechanisms of regulation of LPS driven antibody responses are often interpreted in terms of antigen driven responses even though there is much evidence to suggest that LPS and antigen trigger distinct activation pathways. LPS stimulated antibody responses can be prevented by ligation of the B cell antigen receptor (BCR), while antigen driven responses are shut down by the crosslinking of the BCR to the receptor for the Fc portion of IgG (Fc{dollar}\gamma{dollar}R). The present studies on murine splenic B cells in …
Cesarean Delivery And Socioeconomic Status, Linda Ann Turner
Cesarean Delivery And Socioeconomic Status, Linda Ann Turner
Digitized Theses
Women of higher socioeconomic status have been observed to have higher rates of cesarean delivery, said to be evidence that many cesarean sections are unnecessary. Previous investigations have been conducted in settings where access to obstetrical services may be dependent on ability to pay, and investigators have often not adjusted differences for maternal age, parity and previous cesarean delivery, factors known to confound the association. Whether women of upper or lower socioeconomic status are at increased risk of cesarean delivery for biological reasons has not been determined.;The purpose of this investigation was to examine the association between cesarean delivery and …
Estimation Of Haemodynamic Parameters Using Magnetic Resonance Imaging, Richard Frayne
Estimation Of Haemodynamic Parameters Using Magnetic Resonance Imaging, Richard Frayne
Digitized Theses
Techniques have been both analyzed and developed for the estimation of a number of important haemodynamic parameters (including velocity, volume flow rate and shear rate) using a magnetic resonance (MR) scanner. Accurate estimation of these parameters is important because information about the haemodynamic state may (1) aid our understanding of vascular disease processes; and (2) provide a clinically-useful test for the presence of vascular disease.;The objectives of this thesis are: (1) the development of techniques that are necessary for the accurate and precise simulation of physiological flow waveforms in in vitro MR research; (2) the furthering of our understanding of …
Computing The Modulation Transfer Function Of Magnetic Resonance Imagers, Michael C. Steckner
Computing The Modulation Transfer Function Of Magnetic Resonance Imagers, Michael C. Steckner
Digitized Theses
The quality of images produced by imaging devices of all types can be analyzed and quantified by a variety of metrics. Resolution is an important metric for all imaging devices because if the resolution characteristics do not meet the minimum requirements of an application, the resultant image may be unsuitable and possibly misleading. One resolution metric, called the Modulation Transfer Function (MTF), describes the ratio of input to output signal magnitude as a function of spatial frequency. Application of the MTF is limited to systems whose output scales linearly with input (linearity) and produces the same output image regardless of …
N-Aralkylated Derivatives Of 1-Aminobenzotriazole: Isozyme-Selective Mechanism-Based Inhibitors Of Guinea Pig Cytochrome P-450, Kimberley Janine Woodcroft
N-Aralkylated Derivatives Of 1-Aminobenzotriazole: Isozyme-Selective Mechanism-Based Inhibitors Of Guinea Pig Cytochrome P-450, Kimberley Janine Woodcroft
Digitized Theses
1-Aminobenzotriazole (ABT) and its N-benzyl (BBT), N-{dollar}\alpha{dollar}-methylbenzyl ({dollar}\alpha{dollar}MB), and N-{dollar}\alpha{dollar}-ethylbenzyl ({dollar}\alpha{dollar}EB) derivatives were compared for their potency and isozyme-selectivity for mechanism-based inactivation of guinea pig hepatic and pulmonary cytochrome P450 (P450) in vitro, through the use of isozyme selective substrates for the guinea pig orthologs of rabbit P450 2B4, 1A1, and 4B1 (P450 2Bx, 1A1, 4Bx, respectively). {dollar}\alpha{dollar}MB, {dollar}\alpha{dollar}EB, and to a lesser extent, BBT, selectively inactivated P450 2Bx in pulmonary microsomes from untreated or {dollar}\beta{dollar}-naphthoflavone ({dollar}\beta{dollar}NF)-induced, and hepatic microsomes from phenobarbital (PB)-induced, guinea pigs. P450 loss caused by ABT paralleled the inhibition of enzyme activity in hepatic and pulmonary …
The Molecular Characterization Of Human Monoclonal Antiplatelet Autoantibodies, Gregory Andrew Denomme
The Molecular Characterization Of Human Monoclonal Antiplatelet Autoantibodies, Gregory Andrew Denomme
Digitized Theses
Autoimmune thrombocytopenia occurs in a number of clinical settings including idiopathic or autoimmune thrombocytopenic purpura (ITP), systemic lupus erythematosus (SLE), and the lupus anticoagulant (LAC) syndrome. The etiology of the disease, the scope of the autoantigens targeted, and the epitopes involved in autoimmune thrombocytopenia are largely unknown. ITP has the hallmark of the presence of 'pathogenic' autoantibodies targeting platelet glycoproteins (GP) IIb/IIIa and GP Ib/IX. Associated with the thrombocytopenia in the LAC syndrome is the presence of anti-phospholipid antibodies (approximately 95% of cases). The detection of autoantibodies to a variety of self antigens in non-autoimmune individuals prompted this study to …
Suppression Of Eae In The Lewis Rat By Treatment With Neuroantigen-Containing Liposomes, Colleen Sheila Stein
Suppression Of Eae In The Lewis Rat By Treatment With Neuroantigen-Containing Liposomes, Colleen Sheila Stein
Digitized Theses
Treatment of Lewis rats with intracardiac injections of neuroantigen-containing liposomes significantly reduced the clinical severity of spinal cord-induced experimental allergic/autoimmune encephalomyelitis (EAE). Liposomes containing myelin basic protein (MBP), galactocerebroside (GC), or MBP + GC were effective in this respect, while liposomes containing proteolipid protein were not. The best protection was afforded by liposomes composed of whole myelin, as these ameliorated both the acute and relapsing phases of the disease, with a concomitant reduction in CNS pathology.;Neuroantigen-specific proliferative responses of lymphnode cells (LNC) taken from liposome-treated rats were markedly attenuated in comparison to control LNC, while delayed type hypersensitivity (DTH) reactions …
Occupational Exposure To Styrene: Contribution To Hearing Loss, Andrea M. Sass-Kortsak
Occupational Exposure To Styrene: Contribution To Hearing Loss, Andrea M. Sass-Kortsak
Digitized Theses
Prolonged significant noise exposures are well known to result in permanent hearing loss. However, little is known of the contribution of industrial chemical exposures to hearing loss. Information available, both from animal and human studies, raises the possibility that certain aromatic hydrocarbons are ototoxic. The purpose of this study was to assess whether occupational styrene exposure causes hearing loss in a group of workers in the fibre reinforced plastics manufacturing industry. The hearing acuity of 299 subjects was determined, using pure tone screening audiometry, at the beginning of a single workshift and again at the end of the shift. On …
Isozyme-Selective, Lung-Selective Mechanism-Based Inhibitors Of Cytochrome P-450 And Determination Of P450 Isozyme Specificity For Arachidonic Acid Metabolism, Leah Christine Knickle
Isozyme-Selective, Lung-Selective Mechanism-Based Inhibitors Of Cytochrome P-450 And Determination Of P450 Isozyme Specificity For Arachidonic Acid Metabolism, Leah Christine Knickle
Digitized Theses
1-Aminobenzotriazole (ABT) and its N-benzyl (BBT) and N-{dollar}\alpha{dollar}-methylbenzyl ({dollar}\alpha{dollar}MB) derivatives were compared as isozyme-selective, lung-selective (vs liver) mechanism-based inhibitors of P450 in guinea pigs 4 hr following i.v. administration. Monooxygenase activities selective for guinea pig orthologues of rabbit P450 1A1, 2B4 and 4B1 (1A1, 2Bx and 4Bx, respectively) were determined in pulmonary and hepatic microsomes. BBT and {dollar}\alpha{dollar}MB inactivated pulmonary P450 in an isozyme-selective manner. In non-induced and phenobarbital-induced animals the order of inactivation was 2Bx {dollar}>{dollar} 1A1 {dollar}>>>>{dollar} 4Bx whereas in {dollar}\beta{dollar}-naphthoflavone-induced animals {dollar}\alpha{dollar}MB specifically inhibited 2Bx. BBT and {dollar}\alpha{dollar}MB were also highly selective for the inactivation …
Cardiorespiratory And Peripheral Adaptations After Cardiac Transplantation, Lizanne Marie Bussieres
Cardiorespiratory And Peripheral Adaptations After Cardiac Transplantation, Lizanne Marie Bussieres
Digitized Theses
The cardiovascular, pulmonary and musculoskeletal systems were investigated in seven studies of patients with end-stage heart failure before and/or after receiving a heart transplant. The purposes of the studies were to determine the time course and magnitude of the changes in peak oxygen uptake, cardiopulmonary response to exercise and peripheral skeletal muscle histology and biochemistry after cardiac transplantation. In addition, the physiological basis for the limited exercise capacity and the effect of exercise posture on cardiac performance and secretion of atrial natriuretic peptide was also evaluated in cardiac transplant patients.;In the first study, the peak oxygen uptake almost doubled from …
Characterization Of A Schistosoma Mansoni C(3) Receptor As A Potential Vaccine Candidate, Erika Eliana Silva
Characterization Of A Schistosoma Mansoni C(3) Receptor As A Potential Vaccine Candidate, Erika Eliana Silva
Digitized Theses
Schistosomiasis, a chronic and debilitating parasitic disease, infects an estimated 200 million people and causes 750,000 deaths a year. Therefore, the development of an effective vaccine is of high priority for the control of this disease.;The surface of the syncytial epithelium of Schistosoma mansoni consists of an apical plasma membrane and an overlying envelope. The rapid turnover of these membranes in response to the host's attack is considered to be one of the mechanisms by which this parasite escapes the effects of the host's immune system. Our approach has been to identify surface components which play a pivotal role in …
Contrast-Enhanced Ct Measurement Of Blood-Brain Permeability In Human Brain Tumors, Wai Tong Yeung
Contrast-Enhanced Ct Measurement Of Blood-Brain Permeability In Human Brain Tumors, Wai Tong Yeung
Digitized Theses
A contrast enhanced CT method was developed for measuring the blood-brain permeability (K) and cerebral plasma volume (V{dollar}\sb{lcub}\rm p{rcub}){dollar} in human brain tumors in-vivo. In patient studies, Isovue 300 (iopamidol) was injected intravenously at a dosage of 1 ml/kg patient body weight. It was followed by serial head scanning of the tumor and arterial blood sampling to measure the contrast concentration in tumor and arterial plasma respectively. The leakage of iopamidol into the brain through the blood-brain barrier was modelled as an exchange process between two compartments, namely, the intravascular plasma space and the tissue interstitial space. Using this compartmental …
A Genetic Analysis Of Folate Transport In Chinese Hamster Ovary Cells, T Michael Underhill
A Genetic Analysis Of Folate Transport In Chinese Hamster Ovary Cells, T Michael Underhill
Digitized Theses
Chinese Hamster Ovary cells that are about 50x more resistant to the cytotoxic action of methotrexate than wild-type cells were deficient in the ability to take up methotrexate. In the absence of any exogeneous folates these cells require 100-250x the level of folinic acid as do wild-type cells to support growth at a similar level. Two classes of mutants were distinguishable by their revertability for growth on folinic acid and ability to take-up folic acid. Revertants derived from one class were similar to wild-type cells in their ability to grow in medium containing low levels of folinic acid and in …
Determinants Of Critical Power And Anaerobic Work Capacity In Young And Elderly Men, Thomas James Overend
Determinants Of Critical Power And Anaerobic Work Capacity In Young And Elderly Men, Thomas James Overend
Digitized Theses
Determinants of critical power (CP, the theoretical maximal rate of non-fatiguing work) and anaerobic work capacity (AWC, a finite energetic reserve from intra-muscular sources) were determined in young (n = 13, 24.5 y) and elderly men (n = 12, 70.7 y). The purposes of the four studies were to: (1a) determine and compare CP and AWC in young and elderly men; (1b) determine if CP does represent a true maximal rate of non-fatiguing work; (2) determine thigh component cross-sectional areas (CSA) and volumes; (3) determine the strength and strength/CSA ratios of the knee extensors and knee flexors; and (4) identify …