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- AQP11 (1)
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- CDNA Quantification (1)
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- Frenchman Bay, Maine (1)
- HIF (1)
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- Hypoxia (1)
- Immunohistochemistry (1)
- Ion regulation (1)
- Longhorn sculpin (1)
- Marine longhorn sculpin gills (1)
- Molecular sequence (1)
- Myoxocephalus octodecemspinosus (1)
Articles 1 - 7 of 7
Full-Text Articles in Entire DC Network
Novel Quantification And Localization Of Water And Solute Transporters In The Tissues Of The Spiney Dogfish (Squalus Acanthias), Tolulope B. Ojo
Novel Quantification And Localization Of Water And Solute Transporters In The Tissues Of The Spiney Dogfish (Squalus Acanthias), Tolulope B. Ojo
College of Graduate Studies: Theses & Dissertations
The dogfish, Squalus acanthias is a marine cartilaginous elasmobranch found in the North Atlantic and Pacific oceans. Dogfish synthesize and excrete urea as a product of nitrogen metabolism. They also convert ammonia into urea and retain this urea, such that their plasma is isosmotic or slightly hyperosmotic to the surrounding seawater. To facilitate the regulation of body fluid and to maintain ionic concentrations and osmotic pressure, Dogfish use ion and solute transporters (e.g., NCC, UT-1) and aquaporin water channel proteins. Studies have identified some of the aquaporin genes in the elasmobranch genome, but their functions are mostly uncharacterized. Recent transcriptomic …
Quantification Of Aqp11 Paralog Expression In The Osmoregulatory Organs Of Anguilla Rostrata, Keith C. Kurt
Quantification Of Aqp11 Paralog Expression In The Osmoregulatory Organs Of Anguilla Rostrata, Keith C. Kurt
College of Graduate Studies: Theses & Dissertations
Osmoregulation is facilitated by using an assortment of ion and water channels to assist acclimation to changing conditions and to maintain cellular homeostasis. Euryhaline fish can be found in both seawater and freshwater (SW and FW) environments. Expression of ion and water channels have been experimentally demonstrated to change as a fish acclimates to different environmental conditions. Relatively recently, a new group of water channels has been discovered that are primarily intracellular and includes aquaporin 11 (AQP11). Typically, AQPs are located on the cell plasma membrane to allow water to flow in and out of the cell by osmosis to …
Cellular Coping Mechanisms To Hypoxia In The Longhorn Sculpin (Myoxocephalus Octodecemspinosus) Brain, Kelly Heino
Cellular Coping Mechanisms To Hypoxia In The Longhorn Sculpin (Myoxocephalus Octodecemspinosus) Brain, Kelly Heino
College of Graduate Studies: Theses & Dissertations
The loss of neurological function due to hypoxia remains to be a challenge in many species due to the lack of knowledge and understanding of cellular responses. To investigate cellular responses and survival strategies of the brain during hypoxia and post-hypoxia recovery in a moderate hypoxia-tolerant species, with the hopes of identifying possible therapeutic remedies, thirty Longhorn sculpin, Myoxocephalus octodecimspinosus, were challenged with acute hypoxia (40% O2 saturation for 1 h) and twelve additional sculpin were used as controls (~90% O2 saturation). Fish were sampled at each of the following time points: control/normoxic conditions (T=0 h); hypoxic conditions …
Molecular And Immunohistochemical Identification Of A Sodium Hydrogen Exchanger-2c (Nhe2c) Paralog In The Gills Of Marine Longhorn Sculpin (Myoxocephalus Octodecemspinosus), Demi Brett Rabeneck
Molecular And Immunohistochemical Identification Of A Sodium Hydrogen Exchanger-2c (Nhe2c) Paralog In The Gills Of Marine Longhorn Sculpin (Myoxocephalus Octodecemspinosus), Demi Brett Rabeneck
College of Graduate Studies: Theses & Dissertations
Sodium hydrogen exchanger proteins (NHEs) are members of the cation proton antiporter superfamily (CPA) and are thought to function in fish for maintaining physiological ion concentrations and acid-base balances by excreting excess H+ ions from the body in exchange for Na+ ions. There are many more types of these proteins in teleost fish than in mammals due to putative genome duplication. This study describes a new form of NHE2 in the gills of marine longhorn sculpin, Myoxocephalus octodecemspinosus, designated NHE2c. Sequencing revealed that the NHE2c nucleotide-coding region transcribes a peptide 795 amino acids in length with an estimated molecular weight …
Rh Glycoprotein As An Ammonia Transport Molecule, Matthew B. Phillips
Rh Glycoprotein As An Ammonia Transport Molecule, Matthew B. Phillips
College of Graduate Studies: Theses & Dissertations
Fish use their gills to excrete ammonia in order to eliminate nitrogenous waste. We hypothesize that this mechanism is accomplished by one or more transport proteins in the Rh glycoprotein (RhxG) family. Longhorn sculpin (Myoxocephalus octodecemspinosus) cDNA was amplified using polymerase chain reaction (PCR) and then the PCR products were visualized on an agarose gel. The cDNA from the gel bands was then sequenced and the gene sequence fragments were assembled and completed by rapid amplification of the cDNA ends (RACE). By this process we have obtained large portions of the gene sequences of the four known paralogues located in …
Aquaporin 3 Water Channel Protein Gene Expression In Squalis Acanthias Dogfish Shark, Debra Lee Murray
Aquaporin 3 Water Channel Protein Gene Expression In Squalis Acanthias Dogfish Shark, Debra Lee Murray
College of Graduate Studies: Theses & Dissertations
Aquaporins are a family of membrane proteins that provide channels for the rapid movement of water molecules and some small solutes across the cellular membrane. Since the discovery of the first water channel protein, hundreds of homologous proteins have been found in all forms of life and much has been learned about their complex role in body water homeostasis. This is the first study to use molecular and immunological techniques to demonstrate that an orthologue of mammalian AQP3 is present in the epithelial cells of Squalus acanthias dogfish shark kidney, gills, rectal gland, esophagus, stomach, eye and brain. The full-length …
Characterization Of Na+/H+Exchanger-3 (Nhe3) In The Gills Of Longhorn Sculpin (Myoxocephalus Octodecemspinosus), Curtis Eugene Lanier
Characterization Of Na+/H+Exchanger-3 (Nhe3) In The Gills Of Longhorn Sculpin (Myoxocephalus Octodecemspinosus), Curtis Eugene Lanier
College of Graduate Studies: Theses & Dissertations
Acid-base regulation is a vital mechanism in homeostasis. Fish must maintain optimal physiological acid-base parameters during changes in an aquatic habitat. Environmental factors such as changes in salinity affect fish ability to maintain optimal blood plasma ion concentrations. Ion regulation influences the fish ability to maintain optimal acid-base balance. The Na+/H+ Exchanger-3 (NHE-3) has been shown to be the primary membrane ion transporter responsible for acid-base balance in the mammalian kidney. The objective of this study is to characterize NHE-3 in the gills of longhorn sculpin (Myoxocephalus octodecimspinosus).