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The Generation Of Cytotoxic Tumour Infiltrating Lymphocyte Clones Against Head And Neck Squamous Cell Carcinoma, Henry J. Lapointe
The Generation Of Cytotoxic Tumour Infiltrating Lymphocyte Clones Against Head And Neck Squamous Cell Carcinoma, Henry J. Lapointe
Digitized Theses
73 samples of H&NSCCa were processed for these studies. An enzymatic disaggregation protocol for the flow cytometric analysis of DNA content of tumour whole cell preparations was developed. Analysis of the tumour cell component without the need for incorporation of reference controls provided a more accurate assessment of DNA content of tumour cell populations. Six lines of H&NSCCa were established in vitro from fresh tumour samples. Tumour cell effects on PHA- or IL-2 stimulated proliferation of PBMNCs ranged from 61% and 45% suppression to 4% and 121% enhancement of the proliferative responses respectively. Flow cytometric analysis revealed a down regulation …