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Universal Snp Genotyping Assay With Fluorescence Polarization Detection, T. M. Hsu, X. Chen, S. Duan, R. D. Miller, P. Y. Kwok
Universal Snp Genotyping Assay With Fluorescence Polarization Detection, T. M. Hsu, X. Chen, S. Duan, R. D. Miller, P. Y. Kwok
Open Access Publications
The degree of fluorescence polarization (FP) of a fluorescent molecule is a reflection of its molecular weight (Mr). FP is therefore a useful detection methodfor homogeneous assays in which the starting reagents and products differ significantly in Mr. We have previously shown that FP is a good detection method for the single-base extension and the 5'-nuclease assays. In this report, we describe a universal, optimized single-base extension assay for genotyping single nucleotide polymorphisms (SNPs). This assay, which we named the template-directed dye-terminator incorporation assay with fluorescence polarization detection (FP-TDI), uses four spectrally distinct dye terminators to achieve universal assay conditions. …
Biological Methods For Cell-Cycle Synchronization Of Mammalian Cells, Penny Davis, Alan Ho, Steven Dowdy
Biological Methods For Cell-Cycle Synchronization Of Mammalian Cells, Penny Davis, Alan Ho, Steven Dowdy
Open Access Publications
Understanding the molecular and biochemical basis of cellular growth and division involves the investigation of regulatory events that most often occur in a cell-cycle phase-dependent fashion. Studies examining cell-cycle regulatory mechanisms and progression invariably require cell-cycle synchronization of cell populations. Thus, many methods have been established to synchronize cells at specific phases of the cell cycle. Several of the common methods involve pharmacological agents, which act at various points throughout the cell cycle. Because of adverse cellular perturbations resulting from many of the synchronizing drugs used, other synchrony methods that involve less perturbation of biological systems, such as serum deprivation, …