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The Neonatal Chinchilla Cochlea: Morphological And Functional Study, R. V. Harrison, J. R. Cullen, S. Takeno, R. J. Mount

Scanning Microscopy

The developmental time scale of the cochlea varies from species to species. We investigate here the condition of the neonatal cochlea in the chinchilla, a species increasingly used in auditory research. We have examined the morphology of cochlear hair cells using scanning microscopy, and the development of auditory function during the first postnatal month by monitoring auditory brainstem evoked responses (ABR). We find that although there were some outer hair cell kinocilia present in middle and apical areas, the hair cells otherwise were mature at 24 hours after birth. Furthermore, cochlear auditory thresholds are adult-like at birth. However, whilst there …

The Influence Of Fluorescent Light On The Development Of In Vitro Fertilized Bovine Oocytes, Jared Bunch

Undergraduate Honors Capstone Projects

Advances in in vitro fertilization and in vitro culture techniques have allowed considerable progress in identifying physiological requirements of mammalian embryos. Parrish et al. (11) reported a major breakthrough on in vitro fertilization when his group identified heparin as an important factor for the capacitation of spermatozoa. Capacitation is necessary for fertilization of matured oocytes. During the precoculture era of embryos (prior to 1980), the development of early preirnplantation embryos was vary limited regardless of medium, medium supplement, gas atmosphere, osmolarity or pH used (7). Coculture techniques of fertilized oocytes using somatic cells during in vitro production, particularly of bovine …

Touch-Plate And Statolith Formation In Graviceptors Of Ephyrae Which Developed While Weightless In Space, Dorothy B. Spangenberg, Elisa Coccaro, Russell Schwarte, Brian Lowe

Scanning Microscopy

Ultrastructural studies of the statocysts and touch-plates of graviceptors (rhopalia) of Aurelia ephyrae revealed that (1) touch-plate hair cells are present; and (2) cytoplasmic strands from the hair cell bases extend from the neurite plexus to touch similar strands from the lithocytes. This close association of hair cell neurites and statocysts may have important implications regarding the transmitting and processing of positional information with respect to the gravity vector.

Graviceptors of ephyrae which developed while weightless in microgravity were compared with controls at the ultrastructural level. We found that hair cells of ephyrae which developed in microgravity had fewer lipid …

Development Of An Ovine Genome Map With Emphasis On In Situ Hybridization, Melanie R. Heaton

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Livestock genome maps are used to identify economic trait loci and loci proximal to genes affecting economically important traits. This research contributes to the development of the ovine genome map by establishing techniques to physically map large DNA inserts to ovine chromosomes using fluorescent in situ hybridization. Fluorescent in situ hybridization techniques were established using yeast artificial chromosomes as DNA probes. Probes were as large as 1,000,000 kilobases and hybridized to fixed ovine metaphase chromosomes from a callipyge ewe. Three yeast artificial chromosome probes contained a genetic marker for the ovine callipyge gene. Probes were assigned to ovine chromosome pair …

Development Of An Enzyme Linked Immunosorbent Assay Capable Of Detecting Antibodies Specific To The Cytomegalovirus, Rachel Bird

Undergraduate Honors Capstone Projects

The cytomegalovirus (CMV) is capable of causing serious illness and death in immunocompromised individuals. The objective of this research is to develop a method of detecting antibodies against CMV. This has lead to the development of an Enzyme linked Immunosorbent Assay (ELISA) that is capable of detecting CMV or anti-CMV antibodies. The process in the development of such an assay involves; 1) the purification of Hyperimmune Rabbit Serum containing anti-CMV antibodies, 2) determination of the appropriate concentrations necessary to provide a clear and concise response to the test, and 3) testing of monoclonal antibodies to determine their specificity towards the …

Final Environmental Impact Statement Expanded Moxa Arch Area Natural Gas Development Project, Sweetwater, Lincoln, And Uinta Counties, Wyoming, United States Department Of The Interior Bureau Of Land Management

Final Environmental Impact Statements (WY)

The Expanded Moxa Arch Area Natural Gas Development Project EIS analyzes a proposal by the Moxa Arch operators (Amoco Production Company, Union Pacific Resources Company, Wexpro/Celsius Energy Company, Bannon Energy, Marathon Oil Company, Presidio Exploration, and other companies) to continue to infill drill additional development wells in their leased acreage within the Moxa Arch oil and gas development area. The project area encompasses approximately 476,261 acres of southwestern Wyoming. Lands associated with the additional drilling program include those previously analyzed in the Amoco Production Company Moxa Arch Natural Gas Production Environmental Assessment (EA) and Decision Record (DR) (USDI-BLM 1991) and …

Economic Indicator Maps For Rural Development In The East, United States Department Of Agriculture, Forest Service

Economic Development

Legislation and social policy require the U.S. Department of Agriculture, Forest Service to help diversify the economies and improve the well-being of rural America. This document provides county-level information on 12 economic indicators, including economic diversity and recreation dependency, from both national and regional perspectives for States in the East, including all States east of Texas, Oklahoma, Missouri, Iowa, and Minnesota.

Enron Burly Development Well No. 58-18d Finding Of No Significant Impact And Decision Record, United States Department Of The Interior Bureau Of Land Management

Record of Decisions (WY)

The proposed well, Burly 58-18D, is located on private surface on a steep slope above fogarty Creek and within view of identified crucial elk winter range. The Burly project is located west of Mobil's office on the Calpet Highway between Big Piney and LaBarge, Wyoming.

The Timing Of Land Development: An Invariance Result, Amitrajeet A. Batabyal

Economic Research Institute Study Papers

The Arrow-Fisher-Henry (AFH) analysis of land development under uncertainty has been conducted in a two-period model. Recently, Capozza and Helsley in 1990 and Batabyal in 1995 have addressed the question of land development under uncertainty in a many-period setting. In this paper, I extend aspects of this literature by analyzing the land development question in a Markov decision theoretic framework. Inter alia, I show that the timing of land development is invariant to the manner in which a landowner uses information about the consequences of development.