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The Fate Of Iron Released From Heme By Hemeoxygenase-1, Jonathan Mark Gardner
The Fate Of Iron Released From Heme By Hemeoxygenase-1, Jonathan Mark Gardner
Undergraduate Honors Capstone Projects
A strain of Escherichia coli was genetically modified to co-express human heme oxygenase-1 and ferritin. The E. coli were then grown with varying amounts of hemin to see if the iron released upon degradation of the hemin by heme oxygenase-1 is loaded into ferritin. Following incubation, the ferritin was purified and the amount of iron loaded into ferritin determined. It was found that ferritin purifed from E. coli expressing human heme oxygenase-1 contained more iron than E. coli that did not contain human heme oxygenase-1. It was concluded that some of the iron released upon degradation of hemin by heme …
Oxidative Damage Caused By Iron Loading Into Ferritin, Talina Christensen Watts
Oxidative Damage Caused By Iron Loading Into Ferritin, Talina Christensen Watts
Undergraduate Honors Capstone Projects
Ferritin is the iron storage protein found in humans, animals, plants, fungi and bacteria. We are interested in how iron is loaded and stored in mammalian ferritin. Ferrous iron must be oxidized to ferric iron in order to be stored in ferritin. It is generally believed that ferritin does the loading itself, dependant upon a "ferroxidase activity." Oxidation of iron can result in the production of the hydroxyl radical which can cause oxidative damage to surrounding proteins and other biomolecules. An indicator of oxidative damage to proteins is the formation of carbonyl groups. Using only the H subunit of human …
Bioavailability/Toxicity Of Iron From Aerobically Processed Organic Fertilizer, Stacey Marie Wilson
Bioavailability/Toxicity Of Iron From Aerobically Processed Organic Fertilizer, Stacey Marie Wilson
Undergraduate Honors Capstone Projects
For an undergraduate Honor's project, I worked under the direction of Dr. Jeffery Hall to determine the bioavailability and toxicity of iron from Milorganite® fertilizer, an aerobically processed organic fertilizer. The Milorganite® Company is a subsidiary of the Milwaukee Municipal Sewage District and produces a fertilizer of high iron content (approximately 5-7%). The high iron content has resulted in concern about the toxic potential, which until now was unknown. This thesis paper will explain iron chemistry, bioavailability, requirements, absorption, toxicity, and treatments, followed by a description of fertilizer types and contents. This will be followed by a description and reasoning …
The Role Of Ferritin In Iron Regulation, Rick White Obray
The Role Of Ferritin In Iron Regulation, Rick White Obray
Undergraduate Honors Capstone Projects
Iron plays a critical role in biological systems. Its variable redox properties make it a tremendously versatile element. It is involved in numerous cellular processes including; oxygen transport, nucleotide synthesis, nitrogen fixation, electron transport and a host of metabolic reactions. Without it, nearly all life would cease to function. However, under certain circumstances iron can be extremely detrimental to biological systems and has been linked to numerous pathologies, including cancer, diabetes, Parkinson's disease, Alzheimer's and atherosclerosis. In the presence oxygen, Fe(II) is rapidly oxidized to Fe(III), and oxygen is reduced to potentially deleterious oxygen radicals (1). These radicals, especially the …
Iron-Dithiothreitol Dependent Production Of 8-Hydroxy-2'-Deoxyguanosine In Dna, David Allan Cook
Iron-Dithiothreitol Dependent Production Of 8-Hydroxy-2'-Deoxyguanosine In Dna, David Allan Cook
Undergraduate Honors Capstone Projects
Oxidative damage to DNA in a metal-thiol system was studied. Calf thymus DNA was incubated in an iron(IIl)-dithiothreitol system prepared in various buffers. Damage was measured by monitoring the production of 8-hydroxy-2'-deoxyguanosine. DNA oxidation was proportional to the concentration of dithiothreitol (DTT) in HEPES and tris(hydroxymethyl)aminomethane (Tris) buffers while it was only roughly related to DTT concentration in sodium chloride. Incubation in potassium phosphate buffer produced no damage. The results indicate that hydroxyl radicals may be generated by the iron-OTT system in HEPES, Tris, and sodium chloride, while in the phosphate buffer an iron phosphate complex may be formed which …