Digital Commons Network^™

Object Enhancement, Noise Reduction, Conversion And Collection Of Spatiotemporal Image Data, Michael Izaguirre Jr.

Theses and Dissertations

In this report, a variety of cellular dynamics are enhanced and analyzed utilizing various algorithms and filter for contrast enhancement. This report will also illustrate the underlying complexities of processing compressed data received from certain type of sensors, their default applications, various methods in converting compressed data to compatible universal uncompressed formats allowed in scientific applications, various methods of image and video capture, guidelines in ethical image manipulation, various methods of frame extraction, and analyzing/processing video images. These methods and processes purposely utilize freeware and public domain software to lower the cost of reproducibility for all.

Investigating Limitations To Nitrogen Fixation By Leguminous Cover Crops In South Texas, Stephanie L. Kasper

Theses and Dissertations

Many farms use leguminous cover crops as a nutrient management strategy to reduce their need for nitrogen fertilizer. Biological nitrogen fixation (BNF) depends on a symbiotic relationship between legumes and nitrogen-fixing rhizobia. Under certain conditions despite inoculation with appropriate rhizobial strains, this symbiosis fails to form. Such failure was observed in a 14-acre winter cover crop trial in the Rio Grande Valley of Texas when three legume species (forage pea, crimson clover, and hairy vetch) produced no signs of nodulation and nitrogen fixation. This study examined nitrogen, phosphorus, moisture, micronutrients and biotic interference as potential causes for the failure and …

Effects Of Elevated Temperature On Gonadal Functions, Cellular Apoptosis, And Oxidative Stress In Atlantic Sea Urchin (Arbacia Punctulata), Jackson B. Johnstone

Theses and Dissertations

Increasing surface sea water temperatures effect on growth, reproduction and development in marine organisms. Sea urchins are excellent indicator species and ideal model organisms to focus on. In this study, I tested the effect of higher temperatures on reproductive functions, heat shock protein (HSP, a chaperone protein produced by cells in response to heat stress) and nitrotyrosine protein (NTP, an indicator of reactive nitrogen species, RNS) expressions, cellular apoptosis, and ceolomic fluid (CF, a body fluid which regulates important physiological processes) conditions in Atlantic sea urchin at three different temperatures. Ten sea urchins were placed in each of six aquariums …

The Snail Communities Of The Lower Rio Grande Valley, Briante Shevon Lewis Najev

Theses and Dissertations

The lower Rio Grande Valley are three lowest latitudinal counties of south Texas. This unique, subtropical, semi-arid environment has the last remaining Tamaulipan Thornforest in the USA. The Tamaulipan Thornforest of Texas occupies less than 2% of their former range. Before this habitat possibly dwindles even more there has been a comprehensive survey of terrestrial snails in intact, altered, and urban areas of the valley.

We have found a 16397 of snails in intact sites with a high species richness at 17; followed by altered sites with an abundance of 12143 snails and a species richness of 15. There were …

A Snapshot Of The Age, Growth, And Reproductive Status Of Gray Triggerfish (Balistes Capriscus, Gmelin 1789) On Three Artificial Reefs In The Northwest Gulf Of Mexico, Adam M. Lee

Theses and Dissertations

Age, growth, and reproductive status of gray triggerfish (Balistes capriscus) were identified from 2015-2016 on artificial reefs in the northwest Gulf of Mexico. Individuals ranged from 232-432 mm fork length with and a mean fork length of 319 mm. Individuals from age 0.2 to 5.2 yrs were observed with a weight to length relationship of Wg = 1.1 x -104 x FL2.7 (r2 = 0.94, n = 112), where FL = fork length (mm) and Wg = weight (g). A von Bertalanffy growth equation of Lt = 326(1 - e - 0.9 (t + 1.71)) was calculated irrespective …

Investigating The Efficacy Of Jak2/Stat3 And Pi3k/Mtor Inhibitory Therapy On Glioblastoma And Basal Breast Cancer Cell Lines, Neftali Vazquez

Theses and Dissertations

The JAK2/STAT3 and PI3K/mTOR pathways are upregulated in cancer cells. STAT3 promotes a positive feedback loop for the stem program whereas PI3K inhibits FOXOs tumor suppressor role and mTOR promotes tumorigenic advancement. Particularly GBM and BBC are aggressive cancers that have a stem cell population that makes them difficult to treat and metastatic. We investigate the effects of a combination therapy that inhibits JAK2/STAT3 and PI3K/mTOR output aiming to lower stem cell expression and promote apoptosis. Our collected data indicates that the dual inhibition induced apoptosis, but it also increased the stem cell signature. The findings pave way for the …

Comparative, Lithology-Based Biogeography Of Phreatophytic And Non-Phreatophytic Matrices Along A North-South Gradient Of The South Texas Sand Sheet, Christopher Joaquin Munoz

Theses and Dissertations

A 70km-long, N-S stratigraphic cross-section spanning the entirety of the South Texas Sand Sheet (STSS) has been rendered along Texas Interstate 69C. The cross-section indicates localized zones of substantial STSS thickness (>4m) as well as zones where the underlying Goliad Formation is either exposed or just below the surface (<0.5m). Spatial patterns of both phreatophytic and non-phreatophytic vegetation assemblages were analyzed by means of remote sensing and in situ herbaceous species evaluations. Biogeographic patterns were comprised of three vegetation matrices: P. glandulosa-dominated, Q. virginiana-dominated and a matrix of herbaceous plants. The geographic distribution of vegetation assemblages corresponded to specific lithological parameters and features. Namely, Q. virginiana concentration exhibited a positive linear relationship with the lithological parameter of STSS thickness (P =2.122x10-11) (α = 0.05). P. glandulosa/V. farnesiana …

High-Powered Led Assembly As Replacement For Conventional Hbo Lamps In High Resolution, High Magnification Microscopy System, Christian Y. Miranda Solis

Theses and Dissertations

Through decades, fluorescence microscopy has relied on mercury vapor lamps as its main light source due to their high power, broad spectrum, and bright bands generated. The use of mercury vapor lamps has remained fundamentally associated with the required lighting equipment of research laboratories. However, as time progresses, mercury vapor lamps have been slowly replaced with technologies that offered longer lifetimes, less hazardous waste, and output light at similar intensities. LED technology has been actively used in biological research, but was limited by price, lower intensities, and stability. The recent development of LED technology has allowed for further understanding and …

Effects Of Global Warming On Gonadal Functions, Cellular Apoptosis, And Oxidative Stress In The American Oyster, Sarah B. Nash

Theses and Dissertations

Global warming due to climate change is predicted to intensify the heat stress in marine and coastal organisms, affecting their development, growth and reproductive functions. In this study. I analyzed gonadal development, heat shock protein 70 (HSP70), nitrotyrosine protein (NTP), dinitrophenyl (DNP) expressions, cellular apoptosis, and coelomic fluid (CF) conditions in American oyster. Oysters were placed in six aquariums and exposed to control (24°C), medium (28°C), and high (32°C) temperatures for one week. Higher temperature significantly decreased the number of eggs and sperm. CF protein concentrations also declined compared to control. In contrast, CF pH and HSP70 expression in gonad …

Studies On Regulation Of Dihydrotestosterone Binding To And Releasing From The Androgen Receptor, Tahyra M. Resto Santos

Theses and Dissertations

The androgen receptor (AR) is a nuclear receptor that responds to testosterone and 5α-dihydrotestosterone (DHT) by modulating gene expression in a variety of cells, including prostate and muscle tissues. Studies of small molecule interaction with AR have shown diphenyl compounds inhibiting through a binding site labeled Binding Function 3 (BF3). The goal of this research is to analyze the interaction of the BF3 site with DDT and related compounds. Amino acids that are exposed on the surface of the BF3 that might interact with different compounds were mutated. Mutant proteins were tested experimentally for their impact on the inhibition the …

Social Influences On Vocal Development In A Wild Parrot, Caleb Michael Mand Arellano

Theses and Dissertations

The Social Intelligence Hypothesis (SIH) proposes that navigating complex societies requires increased neural processing to monitor shifting relationships. SIH evolutionary focus of brain size variation in adults originally ignored the developmental frameworks leading to increased sociality. I studied the ontogeny of early sibling interactions in wild Green‐rumped Parrotlets (Forpus passerinus) in a Venezuelan population that exhibited large variation in brood size and engaged in elaborate social interactions and complex social learning during nestling development. To test if nests of varying brood sizes exhibit differences in social complexity, I quantified the number and strength of sibling play partnerships, as a proxy …