Digital Commons Network^™

Necropsy Findings Reveal Potential Threats To Native Florida Chelonian Species, Paige A. Spurlock

OneUSF Summer Undergraduate Research Symposium

Necropsies are vital for providing researchers with more information on the underlying conditions that cause disease and death in wildlife. A standard herpetological necropsy, like any necropsy, requires one to look objectively at each specimen, without preconceived expectations as to the cause, beginning with thorough internal and external examination of organs and tissues. This summer, I conducted 15 Chelonian (turtle and tortoise) necropsies. Species included gopher tortoise (n=11), and seral freshwater turtle species (n=4). Beyond gross examination, I collected cloacal/oral swabs, liver, lung, kidney, spleen, and brain for potential testing. Most were in good condition. We found a range of …

Efficacy Of Monoclonal Antibody Treatment On Lowering Infectious Disease Burden In U.S. Elderly Patients, Lyndsey M. White, Daniel Johnson, A'Niya Jackson, Laiba Majid

OneUSF Undergraduate Research Conference

Older adults in the United States experience a high burden of infectious diseases due to age related immune decline, reduced vaccine responsiveness, and increased comorbidities. Monoclonal antibody therapies are an important strategy for lowering this burden because they provide immediate and highly specific immune protection. This review evaluates the effectiveness of monoclonal antibodies in reducing infectious disease severity in elderly populations and examines how neutrophils and the formation of neutrophil extracellular traps interact with antibody based treatments. Throughout the review, these therapies have been shown to be successful against multiple infectious diseases, and clinical findings suggest that hospitalized elderly or …

Investigating The Role Of The Gut Microbiome In Modulating Immune Responses To Infections, Abhinav Muthiraparambath, Matthew Guzie, Matthew Lim, Aryan Sharma, Julia Mendez, Irelis Orlando, Archi Patel

OneUSF Undergraduate Research Conference

Increasing evidence links the gut microbiome to immune regulation, homeostasis, and infectious outcomes. Although gut dysbiosis is associated with impaired immune responses, the mechanisms linking microbial imbalance to immune dysfunction and diseases remain under-investigated. This study aims to investigate how changes in the gut microbiome contribute to immune dysfunction and disease progression, and whether biomarkers can improve the prediction of disease outcomes and therapeutic responses. A systematic literature review was conducted to identify peer-reviewed studies explaining the relationship between gut microbiome dysbiosis and immune dysfunction. This was conducted by searching select words such as ‘Gut microbiome’, ‘Gut dysbiosis’, and ‘microbial …

Engineering Fsh-Cer T Cells To Enhance Response To Epithelial Ovarian Cancer, Nolan J. Beatty

USF Tampa Graduate Theses and Dissertations

Ovarian cancer remains a significant cause of cancer-related mortality, with epithelial ovarian cancer (EOC) being the most common subtype. Despite advances in treatment, the 5-year survival rate for late-stage EOC remains low due to factors such as tumor heterogeneity and an immunosuppressive tumor microenvironment (TME). This study investigates the therapeutic potential of chimeric endocrine receptor (CER) T cells engineered to express follicle stimulating hormone (FSH) in a syngeneic mouse model of EOC expressing follicle stimulating hormone receptor (ID8-FSHR). We compared two different co-stimulatory domains—CD28ζ and 4-1BBζ—in FSH-CER T cells and found that FSH-CD28ζ CER T cells exhibited enhanced cytotoxicity, proliferation, …

The Isotopic Profile Of Plague Victims Buried On The Quarantine Islands Of Venice, Italy, Katherine L. Dunning

USF Tampa Graduate Theses and Dissertations

This thesis combined carbon, nitrogen, and oxygen stable isotope analysis and historical documentation to explore diet and mobility for 45 individuals whose remains were excavated from the Venetian island Lazzaretto Vecchio, where plague victims were quarantined, cared for, and buried between 1423 and 1700 CE. δ13C from bone collagen, bone apatite, and tooth enamel apatite was evaluated to determine terrestrial, aquatic, C3, and C4 contributors to diet. δ15N from bone collagen was evaluated to understand the individuals’ trophic levels. δ18O from tooth enamel apatite and bone apatite was evaluated to detect mobility over the life course. Adult diet was primarily …

House Sparrow Invasion Success Across Ecological, Immunological, And Molecular Scales, Kailey Marie Mccain

USF Tampa Graduate Theses and Dissertations

Biological invasions offer natural experiments for studying how organisms cope with ecological novelty. Although invasion success is often attributed to enemy release, introduced organisms do not enter enemy-free environments. Instead, they enter heterogeneous landscapes in which some familiar enemies are absent, others remain, and entirely novel enemies may be encountered. The question, then, is how invaders (hereafter non-natives) are poised to respond to these threats? This dissertation examines whether successful invasions in house sparrows (Passer domesticus) is associated with shifts in pathogen exposure, immune dynamics in blood, gut physiology, and gene co-expression network structure.

Chapter 1 is at the population/ecological …

Association Of Social Isolation, Loneliness, Social Strain And Support With Cancer Onset, And Frailty And All-Cause Mortality In People With Cancer, Joanne Elayoubi

USF Tampa Graduate Theses and Dissertations

Cancer is the second leading cause of death both in the United States and globally and for individuals over sixty-five. In 2026, approximately 2,114,850 new cancer cases are projected to emerge according to the National Center for Health Statistics (Siegel et al., 2026). Cancer incidence is increasing, making older adults sixty-five and older at risk of frailty and mortality following cancer. Although social isolation, loneliness, lack of social support, and social strain have been found to be risk factors for poor health and mortality, it is not clear the extent to which social relationships are associated with incident cancer, and …

Photoluminescent Studies Of Metal-Organic Frameworks For Small Molecule Sensing, Julia T. Dematteo

USF Tampa Graduate Theses and Dissertations

Metal–organic frameworks (MOFs) are highly tunable nanoporous materials with broad applications in gas separation, drug delivery, light harvesting, and chemical sensing. The incorporation of photoactive components into MOFs enables the development of optical sensors capable of detecting a wide range of analytes. Porphyrins and metalloporphyrins are particularly attractive for such applications due to their high molar absorptivity, strong emission quantum yields, and versatile interactions with chemical species.

In this work, two porphyrinic MOFs, PCN-222 and MOF1(Cd), are investigated. Both frameworks utilize tetrakis(4-carboxyphenyl)porphyrin (TCPP) as the organic linker, imparting optical functionality to the materials. Integration of TCPP into the framework induces …

Help Needed For A Neotropical Migratory Bird Tick Collection Project, Danielle Kaschube

North American Bird Bander

No abstract provided.

Combating Β-Lactamase Resistance: From Inhibitor Design To Substrate Evolution, Lian M.C. Jacobs

USF Tampa Graduate Theses and Dissertations

β-lactamase production by bacteria is a major mechanism of antibiotic resistance. These enzymes hydrolyze β-lactam antibiotics, preventing them from binding to and inhibiting PBPs. Although new β-lactam scaffolds and inhibitors are continually introduced into the clinic, resistance often emerges within a few years. Therefore, the development of cross-class inhibitors with novel scaffolds is critical to mitigate this resistance. In parallel, a deeper understanding of the structural and mechanistic basis of β-lactamase resistance is essential. Together, these approaches provide an effective strategy for combating antibiotic resistance.

The first project addressed the need of effective cross-class β-lactamase inhibitors capable of targeting SBL …

Field Notes, Bruce G. Peterjohn, Dwight Chasar

Cleveland Bird Calendar

No abstract provided.

Policy Brief For Integrated Perspectives: Florida Ems Workforce And Agency Synthesis 2025, Florida Center For Emergency Medical Services

Florida Center for Emergency Medical Services Research Reports

Executive Summary

Workforce shortages persist. Paramedic vacancies remain widespread across Florida, with a median of two open positions per agency. Clinicians cite dissatisfaction with pay as the most significant retention concern, with compensation receiving the lowest satisfaction rating among all employment domains.

Recruitment pipelines are growing. Agencies report active investment in cadet programs (42 agencies), explorer programs (31 agencies), and high school EMS academy partnerships (24 agencies). However, clinicians emphasize that systemic barriers including low entry-level pay and limited advancement opportunities constrain the long-term effectiveness of these efforts.

Wellness programs are underutilized. A significant gap exists between …

Iron Deficiency And Depressive Symptoms: Effects Of Racial And Ethnic Disparity In Two Reproductive-Aged Us Women, Alexis Monkhouse

USF Tampa Graduate Theses and Dissertations

Problem: Reproductive- aged women are one of the populations most at risk for iron deficiency and depression in the United States. While many studies have looked at the relationship between these two illnesses, none have explored it from a biocultural framework, examining how race/ethnicity affects this relationship. Using data from NHANES, this thesis examined the relationship between iron status (total body iron) and depressive symptoms (PHQ-9) among reproductive-aged women in the U.S. and how race/ethnicity affects it. Methods. NHANES survey data from 2005-2010 were pulled. Demographic and laboratory data were used to determine eligibility, total body iron, and depressive scores. …

Clinical Features, Laboratory Characteristics, And Outcomes Of Covid-19 Patients In A Tertiary Care Reference Hospital In Panama City, Panama. A Retrospective Observational Study, Santiago E. Hernandez Bojorge

USF Tampa Graduate Theses and Dissertations

Latin America experienced some of the world’s highest COVID-19 mortality rates, yet data on in-hospital outcomes and their social and geographic determinants remain limited. This dissertation investigated multilevel baseline factors associated with in-hospital mortality among adults with COVID-19 at Panama’s largest public tertiary hospital (Hospital Santo Tomás), integrating survival, mediation, and spatial-temporal modeling within a causal-inference framework.

A retrospective cohort of 1,526 RT-PCR–confirmed COVID-19 hospitalizations (May 2020–November 2021) was analyzed using multivariable Cox proportional-hazards, Fine–Gray competing-risk, mediation, and Bayesian spatial models. Directed acyclic graphs (DAGs) pre-specified causal relationships and minimally sufficient adjustment sets. DAG-guided models quantified total effects without conditioning …

Targeting Cancer With T Cells: Preclinical And In Silico Insights To Improve Immunotherapies, Nina Obertopp

USF Tampa Graduate Theses and Dissertations

T-cell–based immunotherapies are often constrained by limited trafficking and persistence in tumors and by functional exhaustion under chronic antigen exposure. This dissertation addresses these barriers through two distinct strategies: (i) preclinical studies testing how radiation therapy modulates tumor-infiltrating lymphocytes (TIL) therapy, and (ii) agent based modeling (ABM) to optimize treatment day treatment-free intervals (TFI) in T-cell engager (TCE) therapy. (i) In a human papillomavirus–positive (HPV⁺) head-and-neck cancer (HNC) model, a single local-dose of RT (8 Gy) delivered five days before tumor harvest increased the success and functional quality of ex vivo TIL outgrowth from tumor fragments, enriching polyfunctional CD8⁺ subsets. …

Navigating (M)Otherhood: Perception, Practice, & Policy Of Adolescent Pregnancy In The North Coast Of Perú, Mahir Rahman

USF Tampa Graduate Theses and Dissertations

“It’s a vicious circle”, the obstetricians of Huanchaco repeated time and time again after discussing a recently pregnant 13-year-old girl who had just moved to the North Coast of Perú. Adolescent pregnancy is an extremely stigmatized topic in global maternal and child health discourse. Pregnant adolescents are subject to educational policy, clinical protocol, and community practice. While global scholarship emphasizes adolescent pregnancy prevention, limited scholarship focuses on the conditions in which adolescent pregnancy is perceived, discussed, and managed. More importantly, these perspectives are even less documented amongst the North Coast of Perú.

This thesis serves as an ethnographic exploration of …

Antimicrobial Resistance Of Cultivable Bacteria Isolated From Sorcerer’S Cave In Texas, Jennifer R. Huddleston, David E. Sanderson, Braden J. Smith, Joshua M. Brokaw, Evan M. Babb, Jeffrey T. Wooliscroft

International Journal of Speleology

Studying caves with minimal human contact offers insights into antimicrobial resistance before human influence. In this study, we isolated 315 bacterial strains from Sorcerer’s Cave in Texas and evaluated their resistance to seven common antimicrobials. Under half (41.4%) of the Gram-positive isolates and 68.4% of the Gram-negative isolates were resistant to three or more antimicrobials. The two most common genera identified were Pseudomonas and Bacillus. Compared to bacteria isolated in previous cave studies, Sorcerer’s Cave bacteria exhibited more extensive antimicrobial resistance. This study underscores the importance of minimally disturbed cave environments in advancing our knowledge of antimicrobial resistance present …

Ifnγ-Driven Skewing Towards Th1 Over Th17 Differentiation Underlies Crs And Neutropenia In Car-T Therapy, Payal Goala

USF Tampa Graduate Theses and Dissertations

CAR-T therapy has improved overall and complete response rates, andprolonged progression free survival of patients with relapsed/refractory hematological malignancies. The short-term and long-term impacts of anti-CD19 CAR-T products on the immune landscape of B cell malignancies has garnered considerable attention in recent years. Most notably, a subset of patients experience high-grade cytokine toxicities, including cytokine release syndrome and immune cell-associated hematologic toxicity that manifest as prolonged cytopenia. Whereas etiology of CRS is more well established, the mechanisms leading to CAR-T associated cytopenia remains elusive. Rejeski et. al. showed that severe cytopenia in patients post CAR-T treatment led to frequent infections …

Integrated Perspectives: Florida Ems Workforce And Agency Synthesis 2025, Michael Lozano

Florida Center for Emergency Medical Services Research Reports

The Florida Center for Emergency Medical Services (FL-CEMS) integrated findings from the 2024 EMT and Paramedic Renewal Survey (49,200 clinicians), the 2025 EMS Provider Survey (260 agencies), and 2024 NREMT Cognitive & Certification exam outcomes to provide a comprehensive picture of Florida’s EMS workforce and training environment.

First Report Of A Bat Guano Fire From A Cave In Southeast Asia, Sopark Jantarit, Martin Ellis, Pruet Kalasuwan, Neeranuch Phusunti, Pimonsri Mittraparp-Arthorn, Jiranan Pattano, Arnon Chukamnerd, Turdtun Tong-In, Natthawut Khrueaaunruean

International Journal of Speleology

Fires occurring in cave guano are a rare and unusual phenomenon and the occurrence of such events in Southeast Asia is particularly novel. In this study, we document the first recorded instance of a bat guano fire in a tropical cave at Tham Chao Ram in Sukhothai province, northern Thailand. A bacteriological analysis was made of the guano at the fire site and from a control site within the cave. We give a comprehensive review of global reports on bat guano fires in caves, conduct an initial investigation of the physical factors at the fire site and discuss the potential …

Molecular Screening Of Avian Viruses And Parasites In Wedge-Tailed Shearwaters Ardenna Pacifica And Barau’S Petrels Pterodroma Baraui On Réunion Island, Camille Lebarbenchon, Celine Toty, Solenn Boucher, Audrey Jaeger, Matthieu Le Corre

Marine Ornithology

We investigated Wedge-tailed Shearwater Ardenna pacifica and Barau's Petrel Pterodroma baraui exposure to infectious agents circulating in the western Indian Ocean. Using polymerase chain reaction and serological analyses, we screened for influenza viruses, flaviviruses, coronaviruses, and blood parasites (Haemoproteus, Leucocytozoon, and Plasmodium). No active infections were detected and serological results suggested limited past exposure, with only two Wedge-tailed Shearwaters testing positive for antibodies to the influenza A virus. These findings indicate the limited role of these species as epidemiological reservoirs. However, environmental factors such as tick infestations and isolated breeding habitats highlight the need for continued epidemiological monitoring to support …

Updating The Status Of The Peruvian Pelican Pelecanus Thagus Following Avian Influenza And El Niño Events In Peru, Liliana Ayala, Gabriela Sandoval-Lazaro

Marine Ornithology

The Peruvian Pelican Pelecanus thagus is an endemic species of the Humboldt Current System. Between November 2022 and March 2024, the avian influenza epidemic and El Niño conditions significantly affected the pelican population. This study provides an update on the population size and breeding status of the species in Peru. Pelican abundance decreased by 31.5% to 37.6% within marine protected areas. The number of live pelicans ranged from 1,652 to 46,046 in 2023 and from 14,505 to 52,540 in 2024, based on counts conducted in natural protected areas by Peruvian government officials. Breeding primarily occurred on six islands between October …

Statistical Challenges And Simulation Results For Pilot Clinical Trials, Weiliang Cen

USF Tampa Graduate Theses and Dissertations

Background: The effect size estimated from a pilot trial is often an inaccurate reflection of the true effect size observed in a large trial, leading to either underestimation or overestimation. Published data suggest that effect sizes from large trials are typically smaller than those reported in their corresponding pilot trials. To address this discrepancy, conservative or discount adjustment methods are widely recommended to modify pilot trial effect sizes when calculating sample sizes, thereby maintaining adequate statistical power. This study aims to assess effect sizes from both pilot and large trials and to evaluate the performance of existing adjustment methods.

Methods: …

A Multi-Faceted Study Of Antibiotic-Resistant, Opportunistic Pathogens In Wastewater And Environmental Water, Jeanette Calarco

USF Tampa Graduate Theses and Dissertations

More than 2.8 million infections due to antibiotic-resistant bacteria occur annually in the United States, and more than 35,000 people die as a result. Domestic wastewater (sewage), which contains antibiotic-resistant bacteria and antibiotic resistance genes, is collected at treatment plants for removal of contaminants, e.g. pathogenic microorganisms and nutrients. While wastewater treatment is designed to remove antibiotic-resistant bacteria and other microorganisms through treatment and disinfection, some bacteria may escape treatment. Treated wastewater effluent is generally discharged into surface waters; however, some fraction of the effluent may be used as recycled water. Compromised wastewater collection infrastructure and sewage spills can disseminate …

Exploring The Behaviors Of Women Who Are Mentally Thriving With Autoimmune Disease: A Mixed Methods Approach, Emilie Ann Ellenberg

USF Tampa Graduate Theses and Dissertations

Autoimmune diseases encompass a variety of conditions that impact millions of Americans, with a higher prevalence among women than men. These diseases involve immune system dysregulation, leading to tissue damage and dysfunction. Despite progress in understanding their underlying mechanisms, important aspects remain inadequately understood, such as the relationships among immune dysregulation, medical outcomes, and psychiatric comorbidities. Preliminary qualitative research indicates that perceived diagnostic invalidation leads to the mental health needs among women with autoimmune diseases often being unmet, and behavioral health help-seeking behaviors being reduced. Although perceived diagnostic invalidation can lead to increased mental health symptomology and decreased help-seeking behaviors, …

Strategic Leadership In Security Media: Enhancing Community Safety Against Drug Abuse In Oman, Raed Awashreh, Saif Alshuhoomi,, Viola Gjylbegaj

Journal of Strategic Security

Rapid global development has led to increased security challenges, particularly regarding drug-related crimes. This study examines the role of strategic leadership in security media to enhance community security, focusing on drug abuse in Oman. Despite ongoing efforts to combat drug-related offenses, these crimes remain a significant threat to societal stability. The qualitative research involved in-depth interviews with 14 decision-makers, including 7 experts from Oman’s security media, police, and related institutions. It explores the effectiveness of security media campaigns in raising awareness and reducing drug use. Thematic analysis of the data revealed key challenges, including limited resources, outdated technologies, and insufficient …

Synthetic Plasmodium Antigens, Compositions, And Uses Thereof, John H. Adams, Christopher L. King, Niraj H. Tolia, Edwin Chen, Nichole Diane Salinas, Miriam Thankam George, Francis B. Ntumngia, Samantha Jones Barnes

USF Patents

Provided herein are synthetic P. vivax antigens, antibodies and pharmaceutical formulations and vaccines thereof. Also provided herein are methods of treating and/or preventing Plasmodium infection and/or disease by administering the synthetic P. vivax antigens, antibodies and pharmaceutical formulations and vaccines thereof.

Florida Ems Voices: What Agency Providers Told Us In 2025, Michael Lozano

Florida Center for Emergency Medical Services Research Reports

The 2025 Annual Florida EMS Provider Survey provides a comprehensive overview of Florida’s EMS system, highlighting critical insights into workforce dynamics, resource allocation, medical direction, and service integration across EMS agencies statewide. Conducted from April to May 2025, the survey received robust participation from 249 agencies, covering 74.5% of licensed EMS providers. Key findings underscore regional variability in workforce recruitment, salary scales, mental health support, mobile integrated healthcare (MIH), and preparedness for mass casualty incidents.

Key areas of strategic emphasis include EMT and paramedic mental health and wellness, mobile integrated healthcare (MIH), and local quality improvement (QI) efforts. Notably, recruitment …

Crisis On The Frontlines: Recruitment And Retention Challenges In Ems, Bruce J. Moeller, Michael Lozano

Florida Center for Emergency Medical Services Research Reports

Florida’s emergency medical services (EMS) and fire service agencies face an unprecedented workforce crisis. Compounded by demographic shifts, rising call volumes, and mounting mental health challenges, many EMS agencies are struggling to recruit and retain qualified personnel. Existing models of recruitment—centered on short-term bonuses or messaging emphasizing sacrifice—are failing to resonate with new generations of workers, who prioritize mental wellness, career progression, and work-life balance. Additionally, outdated training pipelines and a decline in volunteerism, especially in rural areas, have weakened the traditional workforce structure, creating gaps that threaten the stability of emergency response systems statewide.

To address these challenges, the …

Some Corrections To The Record On Lemkin’S Early Years: The Evidence From Poland, Zachary Mazur

Genocide Studies and Prevention: An International Journal

This piece addresses some misunderstandings regarding Raphael Lemkin's early years in Poland, his influences and the development of his ideas. While discussing some of the extent published information, I point out what other archival documents from Poland and the League of Nations have to say about Lemkin's past. One of the main points is that Lemkin was an unreliable narrator on his own life, and this has caused confusion.