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University of South Florida

USF St. Petersburg campus Faculty Publications

2020

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Crystal Structure Of Thermus Thermophilus Methylenetetrahydrofolate Dehydrogenase And Determinants Of Thermostability, Fernando Maiello, Gloria Gallo, Camila Coelho, Fernanda Sucharski, Leon Hardy, Martin Würtele Jan 2020

Crystal Structure Of Thermus Thermophilus Methylenetetrahydrofolate Dehydrogenase And Determinants Of Thermostability, Fernando Maiello, Gloria Gallo, Camila Coelho, Fernanda Sucharski, Leon Hardy, Martin Würtele

USF St. Petersburg campus Faculty Publications

The elucidation of mechanisms behind the thermostability of proteins is extremely important both from the theoretical and applied perspective. Here we report the crystal structure of methylenetetrahydrofolate dehydrogenase (MTHFD) from Thermus thermophilus HB8, a thermophilic model organism. Molecular dynamics trajectory analysis of this protein at different temperatures (303 K, 333 K and 363 K) was compared with homologous proteins from the less temperature resistant organism Thermoplasma acidophilum and the mesophilic organism Acinetobacter baumannii using several data reduction techniques like principal component analysis (PCA), residue interaction network (RIN) analysis and rotamer analysis. These methods enabled the determination of important residues for …


Using Law School Faculty Author Profiles To Promote Impact: The U.S. News & World Report Saga Continues, Allison N. Symulevich Jan 2020

Using Law School Faculty Author Profiles To Promote Impact: The U.S. News & World Report Saga Continues, Allison N. Symulevich

USF St. Petersburg campus Faculty Publications

INTRODUCTION: When U.S. News & World Report announced that it would rank law schools’ scholarly impact, U.S. News asked law schools to work with HeinOnline, a legal database, to ensure the accuracy of the database-created faculty author profiles because they would be using Hein’s database to gather citation metrics to measure scholarly impact. DESCRIPTION OF PROGRAM: This practice article describes a project at UNC Chapel Hill in the law library to ensure that their faculty publications were included in HeinOnline’s database and that HeinOnline Author Profiles were accurate. This case study helps librarians tackling either similar law library projects or …


Leapfrogging At Work: Influencing Higher Levels In The Chain Of Command, Sharon Segrest, Martha C. Andrews, Scott W. Geiger, Dan Marlin, Patricia G. Martinez, Pamela L. Perrewe, Gerald R. Ferris Jan 2020

Leapfrogging At Work: Influencing Higher Levels In The Chain Of Command, Sharon Segrest, Martha C. Andrews, Scott W. Geiger, Dan Marlin, Patricia G. Martinez, Pamela L. Perrewe, Gerald R. Ferris

USF St. Petersburg campus Faculty Publications

Purpose – Acts of interpersonal influence are observed throughout organizations, and most typically, in direct supervisor–subordinate relationships. However, researchers have focused less on subordinates bypassing the chain of command and targeting their supervisor’s supervisor with influence attempts. We conceptualize a new term, “leapfrogging,” as subordinates’ attempts to influence and manage the impressions of their supervisor’s supervisor. Here we focus on influencing the target’s perception of likability (the focus of ingratiation) and competence (the focus of self-promotion). This study focuses on its personal and situational antecedents. Design/methodology/approach – Given the central role of social exchange and psychological processes within this phenomenon, …


Literacy Studies At Usf Statement On Anti-Racism, Jenifer Jasinski Schneider, Elizabeth Hadley, Alexandra Panos, Patriann Smith, Janet Richards, Susan V. Bennett, Annmarie Alberton Gunn, Lindsay Persohn, Aimee Frier, Margaret Krause Jan 2020

Literacy Studies At Usf Statement On Anti-Racism, Jenifer Jasinski Schneider, Elizabeth Hadley, Alexandra Panos, Patriann Smith, Janet Richards, Susan V. Bennett, Annmarie Alberton Gunn, Lindsay Persohn, Aimee Frier, Margaret Krause

USF St. Petersburg campus Faculty Publications

No abstract provided.


The First Global Deep-Sea Stable Isotope Assessment Reveals The Unique Trophic Ecology Of Vampire Squid Vampyroteuthis Infernalis (Cephalopoda), A. V. Golikov, F. R. Ceia, R. M. Sabirov, J. D. Ablett, Ian G. Gleadall, Gudmundur Gudmundsson, Henk-Jan T. Hoving, Heather L. Judkins Jan 2020

The First Global Deep-Sea Stable Isotope Assessment Reveals The Unique Trophic Ecology Of Vampire Squid Vampyroteuthis Infernalis (Cephalopoda), A. V. Golikov, F. R. Ceia, R. M. Sabirov, J. D. Ablett, Ian G. Gleadall, Gudmundur Gudmundsson, Henk-Jan T. Hoving, Heather L. Judkins

USF St. Petersburg campus Faculty Publications

Vampyroteuthis infernalis Chun, 1903, is a widely distributed deepwater cephalopod with unique morphology and phylogenetic position. We assessed its habitat and trophic ecology on a global scale via stable isotope analyses of a unique collection of beaks from 104 specimens from the Atlantic, Pacific and Indian Oceans. Cephalopods typically are active predators occupying a high trophic level (TL) and exhibit an ontogenetic increase in δ15N and TL. Our results, presenting the first global comparison for a deep-sea invertebrate, demonstrate that V. infernalis has an ontogenetic decrease in δ15N and TL, coupled with niche broadening. Juveniles are mobile zooplanktivores, while larger …


A Trace Bound For Integer-Diagonal Positive Semidefinite Matrices, Lon Mitchell Jan 2020

A Trace Bound For Integer-Diagonal Positive Semidefinite Matrices, Lon Mitchell

USF St. Petersburg campus Faculty Publications

We prove that an n-by-n complex positive semidefinite matrix of rank r whose graph is connected, whose diagonal entries are integers, and whose non-zero off-diagonal entries have modulus at least one, has trace at least n + r-1.


Antimicrobial And Antibiofilm Activities Of Probiotic Lactobacilli On Antibiotic-Resistant Proteus Mirabilis, Mona Shaaban, Ola A. Abd El-Rahman, Bashair Al-Qaidi, Hossam M. Ashour Jan 2020

Antimicrobial And Antibiofilm Activities Of Probiotic Lactobacilli On Antibiotic-Resistant Proteus Mirabilis, Mona Shaaban, Ola A. Abd El-Rahman, Bashair Al-Qaidi, Hossam M. Ashour

USF St. Petersburg campus Faculty Publications

The emergence of biofilm-forming, multi-drug-resistant (MDR) Proteus mirabilis infections is a serious threat that necessitates non-antibiotic therapies. Antibiotic susceptibility and biofilm-forming activity of P. mirabilis isolates from urine samples were assessed by disc diffusion and crystal violet assays, respectively. Antimicrobial activities of probiotic Lactobacilli were evaluated by agar diffusion. Antibiofilm and anti-adherence activities were evaluated by crystal violet assays. While most P. mirabilis isolates were antibiotic-resistant to varying degrees, isolate P14 was MDR (resistant to ceftazidime, cefotaxime, amoxicillin-clavulanic acid, imipenem, ciprofloxacin, and amikacin) and formed strong biofilms. Cultures and cell-free supernatants of Lactobacillus casei and Lactobacillus reuteri exhibited antimicrobial and …


The Impact Of Covid-19 On Hiv Treatment And Research: A Call To Action, Tiffany Chenneville, Kemesha Gabbidon, Patricia Hanson, Cashea Holyfield Jan 2020

The Impact Of Covid-19 On Hiv Treatment And Research: A Call To Action, Tiffany Chenneville, Kemesha Gabbidon, Patricia Hanson, Cashea Holyfield

USF St. Petersburg campus Faculty Publications

The impact of the COVID-19 pandemic is far reaching, with devastating effects on individuals, communities, and societies across the world. People with chronic health conditions may be at greater risk of contracting or experiencing complications from COVID-19. In addition to illness or death for those who contract the virus, the physical distancing required to flatten the curve of new cases is having a negative impact on the economy, the effects of which intersect with mental health and other existing health concerns, thus affecting marginalized communities. Given that HIV also has a disproportionate impact on marginalized communities, COVID-19 is affecting people …


The Impact Of Covid-19 On Hiv Treatment And Research: A Call To Action, Tiffany Chenneville, Kemesha Gabbidon, Patricia Hanson, Cashea Holyfield Jan 2020

The Impact Of Covid-19 On Hiv Treatment And Research: A Call To Action, Tiffany Chenneville, Kemesha Gabbidon, Patricia Hanson, Cashea Holyfield

USF St. Petersburg campus Faculty Publications

The impact of the COVID-19 pandemic is far reaching, with devastating effects on individuals, communities, and societies across the world. People with chronic health conditions may be at greater risk of contracting or experiencing complications from COVID-19. In addition to illness or death for those who contract the virus, the physical distancing required to flatten the curve of new cases is having a negative impact on the economy, the effects of which intersect with mental health and other existing health concerns, thus affecting marginalized communities. Given that HIV also has a disproportionate impact on marginalized communities, COVID-19 is affecting people …


Influence Of Size, Sex, And Reproductive Status On The Thermal Biology Of Endemic Florida Scrub Lizards, Alison M. Gainsbury Jan 2020

Influence Of Size, Sex, And Reproductive Status On The Thermal Biology Of Endemic Florida Scrub Lizards, Alison M. Gainsbury

USF St. Petersburg campus Faculty Publications

Climate change is impacting species globally, with many populations declining at an accelerated rate toward extinction. Ectothermic species are particularly vulnerable given their reproductive success is linked to environmental temperatures. Studies of the effect of temperature on reproductive success in oviparous squamates have focused mostly on nest temperatures, after eggs are deposited. However, in some species gravid females are known to thermoregulate differently than other adults to increase reproductive success. It is essential to understand what influences the thermal biology of breeding adults in a population to implement targeted conservation strategies. The Florida scrub lizard Sceloporus woodi is an endemic …


Bioaccumulation And Biomagnification Of Potential Toxic Elements (Ptes): An Avicennia Germinans–Uca Rapax Trophic Transfer Story From Jobos Bay, Puerto Rico, Michael Martinez-Colon, Henry Alegria, Hatice Kubra-Gul, Ashley Huber, Perihan Kurt-Karakus Jan 2020

Bioaccumulation And Biomagnification Of Potential Toxic Elements (Ptes): An Avicennia Germinans–Uca Rapax Trophic Transfer Story From Jobos Bay, Puerto Rico, Michael Martinez-Colon, Henry Alegria, Hatice Kubra-Gul, Ashley Huber, Perihan Kurt-Karakus

USF St. Petersburg campus Faculty Publications

In southern Puerto Rico along the coastline bordering the Jobos Bay National Estuarine Research Reserve, environmental encroachment has exposed mangrove forest to different sources of pollution. Potentially toxic element concentrations from the F1Tess (exchangeable), F4Tess (oxidizable), mangrove leaf litter (MLL), and fiddler crab whole body soft tissue were analyzed to assess the fate and transport of pollutants from the environment and its transition into flora-fauna via trophic transfer. Geo-accumulation factor values suggest the bay has experienced limited to no pollution when combining the concentrations of potentially toxic elements extracted from the F1Tess and F4Tess sediment fractions. These geochemical sedimentary compartments …


Ethics As Obligation: Reconciling Diverging Research Practices With Marginalized Communities, Jill Mccracken Jan 2020

Ethics As Obligation: Reconciling Diverging Research Practices With Marginalized Communities, Jill Mccracken

USF St. Petersburg campus Faculty Publications

This article argues that research design is impacted by ideological frameworks, and when conducting community-based participatory research (CBPR), can create challenges and conflicts throughout the Institutional Review Board (IRB) and other institution’s approval processes. I explore the ideological frameworks that underpin conventional and CBPR methodologies to show how collaboration can influence the questions asked and answered, the roles of researchers in the project, and how research findings can better impact the community at the center of the research. I offer a snapshot of our CBPR project with women who were currently and formerly incarcerated and document the challenges we encountered …


Academic Librarian Publishing Productivity: An Analysis Of Skills And Behaviors Leading To Success, Camielle Crampsie, Tina Neville, Deborah B. Henry Jan 2020

Academic Librarian Publishing Productivity: An Analysis Of Skills And Behaviors Leading To Success, Camielle Crampsie, Tina Neville, Deborah B. Henry

USF St. Petersburg campus Faculty Publications

Scholarly publishing continues to be a prominent expectation for many academic librarians. This survey explores characteristics, behaviors, motivations, institutional supports, and educational opportunities that help library practitioners become successful authors. It also looks at perceived confidence in research skills of both novice and experienced librarians. Many librarians show confidence in research activities related to their overall job assignments (literature searching, writing, and such) but find more sophisticated research skills, such as statistical analysis, more challenging. Findings indicate that having additional graduate research experience beyond the library degree, time-management skills, and collaboration with other researchers may provide useful benefits.


Anchoring Change: Using The Kotter Change Management Framework To Analyze & Facilitate Change In Academic Libraries, Colleen T. Boff, Catherine A. Cardwell Jan 2020

Anchoring Change: Using The Kotter Change Management Framework To Analyze & Facilitate Change In Academic Libraries, Colleen T. Boff, Catherine A. Cardwell

USF St. Petersburg campus Faculty Publications

Changes in the higher education landscape are happening more rapidly than ever and require academic libraries to engage with users in new and different ways. Libraries participate in digital scholarship, lead textbook affordability and OER initiatives, create makerspaces, and more. These new and different expectations require library leaders, managers and employees at every level to facilitate change in a variety of situations that range in complexity and are almost always messy. Learn about trends across a collection of twenty change stories in academic library settings, including two- and four-year institutions in the United States and Canada. At the same time, …


Temporal Variability Of Polycyclic Aromatic Hydrocarbons In Deep-Sea Cephalopods Of The Northern Gulf Of Mexico, Isabel C. Romero, Heather L. Judkins, Michael Vecchione Jan 2020

Temporal Variability Of Polycyclic Aromatic Hydrocarbons In Deep-Sea Cephalopods Of The Northern Gulf Of Mexico, Isabel C. Romero, Heather L. Judkins, Michael Vecchione

USF St. Petersburg campus Faculty Publications

As part of the effort to understand the effects of the Deepwater Horizon Oil Spill (DWHOS), we analyzed tissue from five species of midwater oceanic cephalopods in the northern Gulf of Mexico (GoM) during three time periods, including one period sampled fortuitously just before the spill (2010), and two periods sampled after the spill (2011 and 2015–2016). The species, Japetella diaphana, Abralia redfieldi, Histioteuthis corona, Leachia atlantica, and Onychoteuthis banksii were collected in three geographic areas in the GoM (east, south and southeast of the Macondo wellhead). Results indicate a shift in the composition of polycyclic aromatic hydrocarbons (PAHs) in …


Heat, Health And Hatchlings: Associations Of In Situ Nest Temperatures With Morphological And Physiological Characteristics Of Loggerhead Sea Turtle Hatchlings From Florida, Kelsey Alexandra Fleming, Justin R. Perrault, Nicole I. Stacy, Christina M. Coppenrath, Alison M. Gainsbury Jan 2020

Heat, Health And Hatchlings: Associations Of In Situ Nest Temperatures With Morphological And Physiological Characteristics Of Loggerhead Sea Turtle Hatchlings From Florida, Kelsey Alexandra Fleming, Justin R. Perrault, Nicole I. Stacy, Christina M. Coppenrath, Alison M. Gainsbury

USF St. Petersburg campus Faculty Publications

Incubation temperatures, in addition to an embryo’s genetic makeup, are critical in many aspects of adequate sea turtle embryonic development. The effects of high and low incubation temperatures on hatchling quality have been previously examined; however, many of these studies were conducted on relocated or laboratory-reared nests, which do not accurately reflect natural nest temperature fluctuations. To observe the impacts of varying in situ incubation temperatures on loggerhead sea turtle (Caretta caretta) hatchling morphology, various health variables and locomotor performance, temperature data loggers were deployed in 15 loggerhead nests on Juno Beach, Florida, between May and July 2018. Over the …


First-Time Characterization Of Viable But Non-Culturable Proteus Mirabilis: Induction And Resuscitation, Reham Wasfi, G. R. Abdellatif, H. M. Elshishtawy, Hossam M. Ashour Jan 2020

First-Time Characterization Of Viable But Non-Culturable Proteus Mirabilis: Induction And Resuscitation, Reham Wasfi, G. R. Abdellatif, H. M. Elshishtawy, Hossam M. Ashour

USF St. Petersburg campus Faculty Publications

Pathogenic bacteria can enter into a viable but non-culturable (VBNC) state under unfavourable conditions. Proteus mirabilis is responsible for dire clinical consequences including septicaemia, urinary tract infections and pneumonia, but is not a species previously known to enter VBNC state. We suggested that stress-induced P. mirabilis can enter a VBNC state in which it retains virulence. P. mirabilis isolates were incubated in extreme osmotic pressure, starvation, low temperature and low pH to induce a VBNC state. Resuscitation was induced by temperature upshift and inoculation in tryptone soy broth with Tween 20 and brain heart infusion broth. Cellular ultrastructure and gene …