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Glycine N-Acyltransferase-Like 3 Is Responsible For Long-Chain N-Acylglycine Formation In N18Tg2 Cells, Kristen A Jeffries, Daniel R Dempsey, Emma K Farrell, Ryan L Anderson, Gabrielle J Garbade, Tatyana S Gurina, Imran Gruhonjic, Carly A Gunderson, David J Merkler
Glycine N-Acyltransferase-Like 3 Is Responsible For Long-Chain N-Acylglycine Formation In N18Tg2 Cells, Kristen A Jeffries, Daniel R Dempsey, Emma K Farrell, Ryan L Anderson, Gabrielle J Garbade, Tatyana S Gurina, Imran Gruhonjic, Carly A Gunderson, David J Merkler
Chemistry Faculty Publications
Long-chain fatty acid amides are signaling lipids found in mammals and other organisms; however, details of the metabolic pathways for the N-acylglycines and primary fatty acid amides (PFAMs) have remained elusive. Heavy-labeled precursor and subtraction lipidomic experiments in mouse neuroblastoma N18TG2 cells, a model cell line for the study of fatty acid amide metabolism, establish the biosynthetic pathways for the N-acylglycines and the PFAMs. We provide evidence that the N-acylglycines are formed by a long-chain specific glycine-conjugating enzyme, glycine N-acyltransferase-like 3 (GLYATL3). siRNA knockdown of GLYATL3 in the N18TG2 cells resulted in a decrease in the levels of the N-acylglycines …
Analysis Of Differential Secondary Effects Of Novel Rexinoids: Select Rexinoid X Receptor Ligands Demonstrate Differentiated Side Effect Profiles, Pamela A. Marshall, Peter W. Jurutka, Carl E. Wagner, Arjan Van Der Vaart, Ichiro Kaneko, Pedro I. Chavez, Ning Ma, Jaskaran S. Bhogal, Pritika Shahani, Johnathon C. Swierski, Mairi Macneill
Analysis Of Differential Secondary Effects Of Novel Rexinoids: Select Rexinoid X Receptor Ligands Demonstrate Differentiated Side Effect Profiles, Pamela A. Marshall, Peter W. Jurutka, Carl E. Wagner, Arjan Van Der Vaart, Ichiro Kaneko, Pedro I. Chavez, Ning Ma, Jaskaran S. Bhogal, Pritika Shahani, Johnathon C. Swierski, Mairi Macneill
Chemistry Faculty Publications
In order to determine the feasibility of utilizing novel rexinoids for chemotherapeutics and as potential treatments for neurological conditions, we undertook an assessment of the side effect profile of select rexinoid X receptor (RXR) analogs that we reported previously. We assessed pharmacokinetic profiles, lipid and thyroid-stimulating hormone (TSH) levels in rats, and cell culture activity of rexinoids in sterol regulatory element-binding protein (SREBP) induction and thyroid hormone inhibition assays. We also performed RNA sequencing of the brain tissues of rats that had been dosed with the compounds. We show here for the first time that potent rexinoid activity can be …