Digital Commons Network^™

Neutrosophic Wald Distribution With Applications To Reliability Analysis, Afrah Al Bossly, Adnan Amin

Neutrosophic Sets and Systems

No abstract provided.

Engineered Nucleic-Acid Binding Intrinsically Disordered Proteins For Biomedical Applications, Telmo Diez Perez

Biomedical Engineering ETDs

This dissertation explores the use of intrinsically disorder proteins (IDPs) in biomedical applications related to the detection and biosensing of nucleic-acid species. We engineer several low critical solution temperature (LCST) nucleic-acid binding elastin-like polypeptides (ELPs) as model IDPs and study their potential in biomedical application via interactions with short single-stranded (ss) DNA, tRNA and viral RNAs. First, we engineer a simple polycationic ELP capable of recruiting DNA on demand in a DNA-protein-rich condensate via LLPS. Moreover, we develop a modified Flory-Huggins theory to quantify the partitioning of DNA and protein upon coacervate formation with and without salts. We then design …

Synthesis And Reactivity Of Heteroatom Embedded Cycloalkynes In Spaac, Nickel Catalyzed Alkylarylation Of Alkenes, And Carbofluorination Of Alkynes, Namrata Khanal

Chemistry and Chemical Biology ETDs

Part I covers Chapters 1, 2, and 3

cycloaddition reactions, that is utilized in biorthogonal chemistry. This metal free version The strain promoted azide-alkyne cycloaddition (SPAAC) reaction is one of the popular 1,3-dipolar of click reaction offers advantage over copper catalyzed click reaction. Since it eliminates the use of copper that is toxic to cells, it is considered favorable for biocompatible reaction. Due to the increased reactivity with dipoles without the use of metal catalyst, cycloalkynes, in particular cyclooctynes, have been employed as the biorthogonal reagent in biorthogonal chemical reaction for the study of a biological processes in vivo. The …

Heterogeneity Of Gene Trees, Jonathan Nenye Odumegwu Unm

Mathematics & Statistics ETDs

Multilocus phylogenetic studies often show a high degree of gene tree heterogeneity —gene trees that have different topologies from each other as well as from the species tree topology. In some cases, this can lead to studies with hundreds of loci having distinct gene tree topologies. The degree of heterogeneity is expected to increase when there is a high degree of incomplete lineage sorting due to short branches (as measured in coalescent units) in the species tree. Other potential sources of heterogeneity include other biological processes such as introgression, recombination within genes, ancestral population structure, gene duplication and loss, and …

Clinical Performance Of Methylation As A Biomarker For Cervical Carcinoma In Situ And Cancer Diagnosis: A Worldwide Study, Cristiana Banila, Attila T. Lorincz, Dorota Scibior-Bentkowska, Gary M. Clifford, Birhanu Kumbi, Dereje Beyene, Cosette M. Wheeler, Kate Cuschieri, Jack Cuzick, Belinda Nedjai

Pathology Research and Scholarship

The shift towards primary human papillomavirus (HPV)-based screening has necessitated the search for a secondary triage test that provides sufficient sensitivity to detect high-grade cervical intraepithelial neoplasia (CIN) and cancer, but also brings an improved specificity to avoid unnecessary clinical work and colposcopy referrals. We evaluated the performance of the previously described DNA-methylation test (S5) in detecting CIN3 and cancers from diverse geographic settings in high-, medium- and low-income countries, using the cut-off of 0.80 and exploratory cut-offs of 2.62 and 3.70. Assays were performed using exfoliated cervical specimens (n = 808) and formalin-fixed biopsies (n = 166) from women …

A Tale Of Itam Tails: New Insights Into The Molecular Mechanisms That Tune Fcɛri Signaling, William K. Kanagy

Biomedical Sciences ETDs

Immunoreceptor Tyrosine-based Activating Motif (ITAM) receptors are critical in sensing extracellular antigen and initiating an immune response. The ITAM receptor FcɛRI, termed the high affinity IgE receptor, is the primary promoter of inducing the Type 1 allergic response and is primarily expressed in mast cells and basophils. In this dissertation, I worked to determine how early signaling events initiated at the FcɛRI ITAM interface tune downstream signaling outcomes. I began by determining that differential phospho-tyrosine ITAM patterns produce unique recruitment profiles of the accessory kinases proteins Syk and Lyn. I next determined the differential signaling ITAM capabilities of Lyn and …

University Of New Mexico Health Sciences Center Discovery Research Annual Report 2021, Michael Haederle, Sara Mota, Jana Fothergill, Cindy Foster, Rebecca Roybal Jones, Michele Sequeira, Seth Fuller

Research Annual Reports

No abstract provided.

A Cartography Of Roots: An Exploration Of Plant Communication, Place, And Story, Mariko Oyama Thomas

Communication ETDs

This study uses oral history and auto-ethnography to collect thematic data on relationships and communication between plants and people in New Mexico, USA. Western and industrial cultures tend to be plant-blind, which is extremely dangerous in the wake of climate disruption and associated loss of plant biodiversity. This study works to collect and produce a generative landscape of narratives of non-binary relationships between humans and plants. These narratives show a range of hopeful, relational connections between human and more-than-human worlds. Overall results indicated the existence of many positive relationships between plants and people in the Western world, and that these …

Cellular Modification And Artificial Cell Construction, Jimin Guo

Chemical and Biological Engineering ETDs

With all its complexities and different biofunctionalities, a cell is the basic structural and functional unit of all living organisms with the exception of viruses. In this dissertation, I demonstrated gain of function strategies of modifying mammalian cells using silicification and modular nanoparticle (NP) exoskeletons. In addition, I used a modular design concept to build a multifunctional artificial RBC system which can mimic the unique characteristics (e.g. shape, flexibility, the ability to carry oxygen, and long circulation times) of the native RBCs. Together, these cell modification or artificial cell construction strategies are expected to generate new mammalian cell-like structures with …

Acoustofluidics And Soft Materials Interfaces For Biomedical Applications, Frank A. Fencl

Biomedical Engineering ETDs

This dissertation describes fabrication of devices and other tools for biomedical applications through the integration of acoustofluidic systems with bio separation assays, instrumentation components, and soft materials interfaces. For example, we engineer a new class of transparent acoustic flow chambers ideal for optical interrogation. We demonstrate efficacy of these devices by enhancing the signal for high throughput acoustic flow cytometry, capable of robust particle focusing across multiple parallel flowing streams. We also investigate an automated sampling system to determine the parameters of transient particle stream focusing in between sample boluses and air bubbles to model a high throughput, multi-sampling acoustic …

Development Of Functional Chemical Probes For The Study Of Viscosity, Fe(Ii), And Ferroptosis And Photo-Triggered Drug Delivery, Yongyi Wei

Chemistry and Chemical Biology ETDs

Small molecule probes are useful tools for the study of biology. In particular, the dye derived fluorescence probes enable to spatiotemporally monitor the events of analytes of interest. The noninvasive feature is particularly attractive for the biological studies in live cells. The challenge is to develop chemical probes capable of detection of the analyte of interest with high specificity.

Toward this end, my Ph. D. study centers on the development of novel chemical probes for the study and understanding of the alternation of important cellar contents and substances and their functions and relationship between normal and disease states. In the …

Sampling Strategies For Finite Population Using Auxiliary Information, Florentin Smarandache, Rajesh Singh

Branch Mathematics and Statistics Faculty and Staff Publications

The present book aims to present some improved estimators using auxiliary and attribute information in case of simple random sampling and stratified random sampling and in some cases when non-response is present. This volume is a collection of five papers, written by seven co-authors (listed in the order of the papers): Sachin Malik, Rajesh Singh, Florentin Smarandache, B. B. Khare, P. S. Jha, Usha Srivastava and Habib Ur. Rehman. The first and the second papers deal with the problem of estimating the finite population mean when some information on two auxiliary attributes are available. In the third paper, problems related …

Readup Buildup. Thync - Instant Α-Readings, Florentin Smarandache

Branch Mathematics and Statistics Faculty and Staff Publications

No abstract provided.

Evaluating The Utility Of Natural History Collections In Research And For The Public, Bethany L. Abrahamson

Biology ETDs

Natural history collections (NHCs) are useful in many fields of study, but knowledge about how they are used in the modern day is poor. The first part of this study, focused on the collections data held by the Museum of Southwestern Biology (MSB), analyzes specimen-based publications, specimen loan records, and guestbook information to determine how NHCs have been used in scientific study over time. Using a novel methodology, I objectively sorted the publications into ten research subjects via particular phrases found in publication titles, places of publication, and keywords. I observed from this dataset the proportions of particular subjects for …

Imaging Interferometric Microscopy To The Limits Of Available Frequency Space, Alexander Neumann

Optical Science and Engineering ETDs

Imaging interferometric microscopy (IIM) is a synthetic aperture approach offering the potential of optical resolution to the linear systems limit of optics (~lambda/4n). IIM allows one to resolve structures not accessible in a conventional illumination setup, while using a low NA microscope objective and thus keeping the large working distance, depth of focus and field of view associated with the lower NA. The goal of this dissertation is to reach ultimate resolution limits of non-fluorescent microscopy by using IIM in new optical configurations realizing a solid immersion technique with immersion materials employed in advanced regimes unsuitable in other systems. The …

Development Of Novel Reaction-Based Fluorescent Probes, Weimin Xuan

Chemistry and Chemical Biology ETDs

Fluorescent probes have been proved to be very valuable tools in chemical and biological applications, and extensive exploration has been made. Recently, reaction-based fluorescent probes became a new design trend and displayed great potential with remarkable implementation to the conventional design method. However, this field is still underdeveloped, and a lot of problems remain to be solved. As part of our groups research interest, my Ph.D. study centered on the development of new reaction-based fluorescent probes. Firstly, our previous research on Hg²⁺ was continued. In this work, we developed a novel ratiometric fluorescent probe for Hg²⁺ with impressive …

Quantification And Propagation Of Nuclear Data Uncertainties, Michael Rising

Nuclear Engineering ETDs

The use of several uncertainty quantification and propagation methodologies is investigated in the context of the prompt fission neutron spectrum (PFNS) uncertainties and its impact on critical reactor assemblies. First, the first-order, linear Kalman filter is used as a nuclear data evaluation and uncertainty quantification tool combining available PFNS experimental data and a modified version of the Los Alamos (LA) model. The experimental covariance matrices, not generally given in the EXFOR database, are computed using the GMA methodology used by the IAEA to establish more appropriate correlations within each experiment. Then, using systematics relating the LA model parameters across a …

Unm Health Sciences Center Annual Report Of Contracts And Grants, 2013, Luke Frank, Paul Akmajian, Sara Mota, Michael Haederly, Kara Mckinney, Heidi Rishel Brakey, Rebecca Gustaf

Research Annual Reports

No abstract provided.

Methods Of Cellular Integration And Techniques For Improving Encapsulated Cell Viability For The Purpose Of Developing Living Materials And Cell Based Bio-Sensing Materials, Travis J. Savage

Chemical and Biological Engineering ETDs

Living materials offer the potential for detection-reporter systems based on living cells that are genetically tailored to sense target analytes with high levels of specificity and accuracy. A biotic or living material would allow the development of simple, hand held devices that could be utilized for many applications including industrial process monitoring, environmental remediation, military and defense applications and drug delivery. Many living materials have been developed and characterized but they still lack heightened and prolonged encapsulated cell viability necessary to make the material a candidate for widespread use. One particular material offers several advantages to increase yeast cell viability …

The Evaluation Of Erk1 Dimerization Models Using Fluorescence Correlation Spectroscopy And The Development Of Analysis Algorithms For Single-Molecule Super Resolution, Fang Huang

Physics & Astronomy ETDs

In this dissertation, we describe three advancements in the area of fluorescence spectroscopy and microscopy. First, we have implemented Fluorescence Correlation Spectroscopy on a home-built confocal microscope. We proposed F/N analysis which stands for the ratio between average intensity and average number of moving objects in FCS as a method to detect dimerization of fluorescently tagged proteins diffusing in three dimensions. We used the technique to evaluate the dimerization hypothesis of ERK1 protein during nucleus translocation and it shows that ERK1 nucleus translocation is dimerization independent. Second, we have developed a multi-emitter analysis algorithm for single-molecule super resolution techniques that …

Effects Of Non-Planar Distortions In Porphyrin Compounds On Their Properties And Functionality, Raid Haddad

Chemical and Biological Engineering ETDs

Porphyrins and related compounds are ubiquitous in nature, performing diverse functions including solar energy transduction, electron transport, molecular transport and storage, and catalysis. Due to their unique photophysical and chemical properties, they are excellent candidates for application as photosensitizers, catalysts, photocatalysts, molecular electronics and opto-electronics. Several properties of porphyrins are sensitive to small variations in their structure, and this dissertation investigates the effects of out-of-plane distortions of the macrocycle on optical properties, reactivity to axial ligands, and substituent rotation. The structure-functions relationships are examined using molecular simulations, UV-visible absorption spectroscopy, resonance Raman spectroscopy, and normal-coordinate structural decomposition (NSD) analysis of …

The Role Of Bcl-2 Modifying Factor (Bmf) In Airway Epithelial Cell Death, Amelia H. T. Unione

Biomedical Sciences ETDs

One of the components that obstruct the airway in asthma is the sudden and increased secretion of mucus from metaplastic mucous cells in small airways. Because previous studies have shown that the resolution of mucous cell metaplasia (MCM) during prolonged exposure of mice to allergen is mediated by IFN gamma and the Bcl-2 family of proteins, we investigated the regulation and the role of a pro-apoptotic BH3 domain-only protein, Bmf, in airway epithelial cells (AECs) treated with IFN gamma. We hypothesize that Bmf is crucial for IFN gamma-induced cell death. Our findings show that IFN gamma suppressed Bmf expression in …

Contract And Grant Awards Fiscal Year 2008, Office Of The Vice President For Research

Office of the Vice President for Research

I invite you to read this report Contract & Grant Awards, fiscal year 2008, which lists contract and grant UC&GV awards received by the University of New Mexico (UNM) during the period from July 1, 2007 - June 30, 2008 (FY08). These awards represent new funds that were acquired during FY08 by the main campus, branch campuses, education centers, and the Health Sciences Center (HSC). The HSC includes the School of Medicine, the College of Nursing, and the College of Pharmacy. The awards received for FY08 total $303.4M, of which $169.3M is attributed to the main campus and $134.1M to …

Tendencies And Perspectives Of Social Sciences In Health In Latin America, Everardo D. Nunes

English

No abstract provided.

Historians And Archaeologists: Proposals For Connecting In A Common Past, Mark E. Harlan

New Mexico Historical Review

No abstract provided.

Annual Report Of The University, 2005-2006, Volumes 1-7, University Of New Mexico

UNM Annual Reports

PROPOSED POLICIES The Office of Government & Community Relations is in charge of advancing the University's interests at all levels of federal, state and local government. The following policy guidelines for working with University units will achieve a coordinated and effective institutional advancement program. • To inform the Office of Government & Community Relations of all planned contacts and correspondence with elected officials and policy-making employees of federal, state and local government, including those who are alumni or friends of the University. Those items which pertain to sponsored research should be coordinated with the Vice President for Research. • To …

Contract And Grant Awards Fiscal Year 2006, Office Of The Vice President For Research

Office of the Vice President for Research

I invite you to read this report Contract & Grant Awards, fiscal year 2006, which lists contract and grant (C&G) awards received by the University of New Mexico (UNM) during the period from July 1, 2005 - June 30, 2006 (FY06). These awards represent new funds that were acquired during FY06 by the main campus, branch campuses and education centers, and the Health Sciences Center (HSC). The HSC includes the School of Medicine, College of Nursing, and College of Pharmacy. The awards received for FY06 total $298.6M, of which $165.4M is attributed to the main campus and $133.2M to HSC. …

The Need For A Comprehensive Approach To Protecting Rare Plants: Florida As A Case Study, Kevin E. Regan

Natural Resources Journal

No abstract provided.

Section 7 Of The Endangered Species Act And The Art Of Compromise: The Evolution Of A Reasonable And Prudent Alternative For The Animas-La Plata Project, Hannah Gosnell

Natural Resources Journal

No abstract provided.

Rationale For A Focus On Biomphalaria Glabrata, Coen M. Adema, Eric S. Loker, Fred Lewis, Matty Knight

Biomphalaria Genome Initiative

No abstract provided.