Digital Commons Network^™

Avian Influenza And The Approach To Designing An H5n1 Vaccine, Lauren N. Hickey

Honors Program: Senior Projects (Public)

The Avian influenza virus (AIV) H5N1 has been responsible for the culling and deaths of millions of birds since 2022 with concern for wildlife, livestock, and human infections occurring as well. With eggs flying off the shelves in grocery stores, even individuals not involved in the agricultural industry have seen the drastic effects of this disease first-hand. For chickens, this virus works quickly and overpowers the innate immune system before the adaptive immune system has time to properly respond. Therefore, a safe and effective vaccine could reduce the number of poultry deaths and prevent the continuous spread of the infection. …

Evaluating The Impact Of Research Equipment Availability On Scientific Rigor, Reproducibility, And Innovation Across Stem Disciplines, Lyndi G. Hraban

Honors Program: Senior Projects (Public)

Research infrastructure plays a central role in shaping the quality and innovative capacity of scientific research. This study evaluates how the availability of research equipment influences scientific rigor, reproducibility, and innovation across STEM disciplines. Drawing on literature from science-to-science research, policy studies, and discipline-specific analyses, this project synthesizes existing scholarship to explore the relationship between access to infrastructure and key research outcomes. Findings across the literature indicate that access to advanced and well-maintained equipment can strengthen scientific rigor by enabling more precise measurements, improved experimental control, and the implementation of standardized research protocols. Shared and centralized facilities are also associated …

Historical Chaos Among Tetrabothriideans (Eucestoda) Of Odontocetes (Artiodactyla)—Revising Generic Taxonomy And Species Diversity For Cestodes Among Oceanic Dolphins, Eric P. Hoberg, Kaylen Marie Soudachanh, Robert L. Rausch

MANTER: Journal of Parasite Biodiversity

Explorations over the past 150 years that have defined the diversity and structure of the tetrabothriidean cestode fauna among Odontocete cetaceans (Artiodactyla) are summarized and reviewed. Persistent errors in interpretation of comparative morphological characters, inconsistent taxonomic decisions, and an understanding of generic-level and species diversity are addressed. We outline a revision of the Tetrabothriidea, which now encompasses 8 genera. Prosthecocotyle is resurrected from synonymy with Tetrabothrius, and Cetaceobothridius n. gen is proposed. A clear basis is outlined for differentiation of generic and species diversity among Cetaceobothridius, Priapocephalus, Prosthecocotyle, Strobilocephalus, Tetrabothrius, and Trigonocotyle that constitute the tetrabothriidean fauna of odontocetes; species …

Genomic Analysis Reveals New Subspecies Of Cercyonis Scudder, 1875 (Nymphalidae: Satyrinae: Satyrini), Jing Zhang, Qian Cong, Jinhui Shen, Leina Song, Todd L. Stout, Michael S. Fisher, Robb Hannawacker, Nick V. Grishin

The Taxonomic Report of the International Lepidoptera Survey

Genomic analysis of Cercyonis (Cercyonis) Scudder, 1875 (Nymphalidae Rafinesque, 1815) partitions the subgenus into three species groups and reveals five new subspecies from the USA (type localities in parentheses): Cercyonis (Cercyonis) pegala koosharem Stout and Grishin, ssp. n. (Utah, Sevier Co.), Cercyonis (Cercyonis) pegala lasala Grishin, M. Fisher, and Hannawacker ssp. n. (Utah, Grand Co.), Cercyonis (Cercyonis) pegala hortoni Grishin, ssp. n. (Arizona, Gila Co.), Cercyonis (Cercyonis) meadii maculana Grishin, ssp. n. (Colorado, Montezuma Co.), and Cercyonis (Cercyonis) meadii rimana Grishin, ssp. n. (Arizona, Yavapai Co.). A neotype is designated for Satyrus boopis Behr, 1864 (USA, California, Contra Costa Co.). …

From Prairie To Practice: Environmental Controls And A Forensic Taphonomic Framework In Southeastern Nebraska, Jessica F. L. Gammage

Nebraska Academy of Sciences: Programs and Proceedings

Forensic taphonomy examines the postmortem processes that affect human remains, but much of the existing research has been developed in environments that differ significantly from those encountered in the Central Great Plains. This creates limitations when applying established models to regions like southeastern Nebraska, where decomposition is shaped by tallgrass prairie ecosystems, loess-derived soils, and strong seasonal variability. Addressing this gap is important for improving the accuracy and reliability of medicolegal interpretations.

This study evaluates how environmental conditions influence decomposition in southeastern Nebraska and proposes a regionally grounded framework for forensic taphonomic research. Drawing on existing literature, regional ecological data, …

The Use Of Chitosan-G-Poly (Acrylic Acid-Co-Acrylamide) In The Delivery Of Imatinib In The Small Intestine, Dawson Ohrt, Clint Everard

Nebraska Academy of Sciences: Programs and Proceedings

The use of hydrogels as drug delivery systems is the future of biotechnology, hydrogels can offer a new way of controlled delivery to specific areas of the body. Hydrogel also create new possibilities for treatments of gastrointestinal stromal tumors (GISTs). There are many different types of hydrogels that can be used in drug delivery. Hydrogels offer customizability in regard to the release of drugs in specific environments. The chemical properties of hydrogels allow for them to be manipulated for specific functions such as release at a specific pH . The hydrogel being used in the study is Chitosan-G-Poly (Acrylic Acid …

Comparing Non-Invasive Survey Methods For Southern Flying Squirrels (Glaucomys Volans) At The Western Edge Of Their Range, Jay E. Winfield, Jeremy A. White

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The southern flying squirrel (Glaucomys volans) is a threatened species in Nebraska that occurs along the Missouri River in the southeastern corner of the state. Recently, a population was discovered in the city of Lincoln, but it is unknown whether this population represents a natural range expansion from the Missouri River population or whether it is isolated and results from a human-assisted movement. A survey of southern flying squirrels in Nebraska is needed, but surveys of this elusive species are challenging. This study investigated and compared two noninvasive survey techniques for rapid detection of flying squirrels: acoustic detectors …

High Biochar Rate Improves Water Retention In Some Environmentally Sensitive Soils, Samundra Sigdel

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Improving water retention and other hydraulic properties is key to managing soil water and sustaining crop production under fluctuating and extreme weather events. We assessed how wood biochar (836 g C kg^-1) applied at 0, 6.25, 12.5, 25, and 50 Mg ha^-1 affected: i) soil hydraulic properties after 5 years and ii) corn and soybean yield, and biomass production of cereal rye cover crop (CC) and CC mix (cereal rye, radish, and winter pea) in sandy, sloping, and semi-arid soils in the central U.S. Great Plains over 6 years. Biochar affected field saturated hydraulic conductivity (K_{sat …}

Beyond The Cycle: How Menstruation Influences Gender Identity And Well-Being In Binary And Non-Binary Adults, Rin Nguyen

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Approximately 1.8 billion people worldwide menstruate, including transgender and gender-diverse (TGD) people, yet their experiences remain underrepresented in research. Given that menstruation can affect gender alignment and psychological well-being, understanding its impact on TGD people is critical. Gender congruence, or alignment between one’s gender identity and expression, is a key factor influencing well-being, with research linking it to reduced depression and anxiety. Furthermore, emerging research on gender euphoria, the joy experienced when gender congruence is experienced, suggests it may serve as a protective factor for mental health and well-being. Gender euphoria has been associated with reduced psychological distress and increased …

Stephen Baenziger, Unl Agronomy & Horticulture, Breeding New Wheat Varieties, Mark Griep, P. Stephen Baenziger

UNL ORCA Interview Series

Stephen Baenziger awarded the ORCA in 2011. He was born in 1951 in Toronto, Canada. When he was an infant, his family immigrated to the U.S., where he grew up in Des Plaines, Illinois. He earned a bachelor's in biological chemistry magna cum laude from Harvard University in Cambridge, Massachusetts. Next, he earned a master's and doctorate in plant breeding and genetics at Purdue University in West Lafayette, Indiana. Over the next decade, he worked for the USDA at Beltsville Agricultural Research Center in Maryland and then at the Monsanto Corporation in St. Louis, Missouri. In 1986, he joined the …

“I Never Really Thought That Would Be Something To Talk About With My Doctor”: Developing A Theory-Led Decision Aid For College Students’ Substance Use Disclosure In Healthcare, Hayley Stahl

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

College students who use drugs face compounding barriers to disclosing substance use to healthcare providers, including uncertainty about health consequences and stigma-driven identity concerns. Existing disclosure tools address neither barrier in an integrated fashion. This explanatory sequential mixed-methods study examined how constructs from the Theory of Motivated Information Management (TMIM; Afifi & Weiner, 2004) and Stigma Management Communication (SMC; Meisenbach, 2010) theory suggest factors influencing substance use disclosure among college students and shape their lived disclosure experiences. In Study 1, simultaneous-entry multiple regression analyses revealed that communication efficacy was associated with information seeking, perceived benefits of disclosure were linked to …

Nebraska And The Midwest Agtech Ecosystem: Benchmarking Innovation Investment And Translation Gaps, Ankit Chandra, Ishani Lal, Brennan Costello

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Agricultural innovation is central to the sustainable transformation of food systems, yet the capital that finances it is unevenly distributed relative to where food is produced. This paper develops a diagnostic framework for measuring this mismatch and applies it across the U.S. Midwest from 2021 to 2025, with particular emphasis on Nebraska's structural position within this landscape. Drawing on venture capital deal-flow data, 17 stakeholder interviews, and a novel strength-weakness-opportunity-gap (SWOG) ecosystem mapping methodology, we document $736 million in tracked Midwest AgTech funding across eight states (Nebraska, Iowa, Missouri, Illinois, Indiana, Kansas, North Dakota, and South Dakota). We introduce three …

Personalizable Metabolic Models To Enable Immune Characterization And Drug Discovery, Joshua Loecker

Department of Biochemistry: Dissertations, Theses, and Student Research

Cellular metabolism is reshaped by disease and physiological state, and genome-scale metabolic networks (GSMNs) offer a systematic way to interrogate this biology and identify therapeutic targets. Building a GSMN from expression data, however, requires a sequence of stages including read processing, normalization and gene-activity classification, multi-omic integration, model reconstruction, simulation, and biological interpretation. These are typically handled by disconnected tools spanning multiple programming languages, file formats, and databases. The resulting reliance on heterogeneous interfaces and manual steps threatens reproducibility. This work develops an ecosystem of computational tools that carry an analysis from raw sequencing reads to mechanistic biological interpretation. First, …

Evaluating Variety- And Market-Class-Specific Effects Of Dietary Pulses On Human Health, Jiayu Tang

Department of Food Science and Technology: Dissertations, Theses, and Student Research

While pulses are globally recognized for their high levels of dietary fibers, protein, and polyphenols, research often focuses on characterizing the physiological effects of a single variety. This approach overlooks the substantial genetic and compositional diversity that exists across pulse varieties and individual market classes, which can differ extensively in nutrient content and structure. These compositional differences have the potential to produce distinct physiological and microbial responses in humans, yet variety- and market-class-specific effects remain poorly characterized.

This thesis uses two complementary research methodologies to investigate the health-relevant effects of different pulse varieties and market classes, providing foundational knowledge to …

Evaluation Of A Vortex Flow Device On A Center Pivot Irrigation System, Ankit Chandra, Derek M. Heeren, Tyler Smith

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

This study presents the first independent field evaluation of a passive vortex flow device designed to reduce hydraulic friction in pressurized irrigation systems. Experiments were conducted on a full-scale, groundwater-fed center pivot irrigation system at the Eastern Nebraska Research, Extension, and Education Center (ENREEC). A paired comparison approach was used to quantify differences in flow rate, energy consumption, and pressure distribution along the pivot lateral under baseline (no device) and treatment (device installed) conditions. Results showed negligible change in system flow rate (621 vs. 618 gpm) and energy consumption (~1.2% difference), consistent with expectations for pressure-regulated irrigation systems. However, the …

Exploring Gene Regulatory Neural Network Biocomputing Of Bacteria, Adrian Merle Ratwatte

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Artificial Intelligence (AI) has evolved from brain-inspired algorithms into a discipline that increasingly integrates with biological systems. While silicon-based platforms have advanced machine learning, they remain limited in energy efficiency and operation in environments beyond silicon. This motivates biological computing as an alternative, enabling efficient, resource-aware, and reconfigurable computing within living systems. This dissertation addresses these limitations by introducing a bacterial computing framework that models Gene Regulatory Networks (GRNs) as Gene Regulatory Neural Networks (GRNNs). The GRNN mirrors the structure and function of Artificial Neural Networks (ANNs) through gene-gene interactions across trans-omic layers, enabling natural, self-regulating information processing within living …

Lost In The Weeds No More! Revision Of The Robust Herb Gall Wasps (Hymenoptera, Cynipidae, Aulacideini) In North America, Louis F. Nastasi, Charles K. Davis, José Luis Nieves-Aldrey, Cecil N. Smith, Simon Van Noort, Ebony G. Murrell, Timothy S. Frey, Chris Friesen, M. J. Hatfield, Heather M. Hines, John F. Tooker, Andrew R. Deans

Department of Entomology: Faculty Publications

We revise the North American fauna of Aulacideini (Hymenoptera: Cynipidae) using morphological, ecological, and genetic (DNA barcode) data, incorporating observations from over 5,000 adult specimens and galls. Prior to revision, the fauna included 24 species in four genera. Six new genera and 25 new species are described, all authored by Nastasi: Argyrix gen. nov., Barathria gen. nov., Cosmita gen. nov., and Ketsia gen. nov., for species previously placed in Antistrophus Walsh; Prixima gen. nov., for a new species inducing galls on Pyrrhopappus DC.; and Tookeria gen. nov., for a new species inducing galls on Silphium L.; Antistrophus frieseni sp. nov., …

Butterfly Taxa Discovery Via Genomic Analysis, Jing Zhang, Qian Cong, Jinhui Shen, Leina Song, Nick V. Grishin

The Taxonomic Report of the International Lepidoptera Survey

Expanding a genomics-guided assessment of butterfly taxonomy, we integrate phylogenetic evidence from all protein-coding genes with morphological considerations to refine our understanding of butterfly systematics and discover new taxa. As a result, 2 subgenera, 39 species, and 5 subspecies are proposed as new (type species in original combinations or type localities are listed in parentheses): Erebia (Magda) magdalena uintana Grishin, ssp. n. (USA: Utah, Uintah Co.) in Nymphalidae Rafinesque, 1815; Myselasia rondea Grishin, sp. n. (Brazil: Rondônia), Myselasia pseudocrinon Grishin, sp. n. (Ecuador: Pastaza), Myselasia paramatuta Grishin, sp. n. (Ecuador: Morona-Santiago), Euselasia anapellos Grishin, sp. n. (Peru: Loreto), …

Interview With Orca Winner John Hibbing, Political Science; How Political Attitudes Correlate With Physiological Traits, Mark A. Griep, John Hibbing

UNL ORCA Interview Series

Dr. John Hibbing was awarded the ORCA in 1993. John was born in 1953 in Paullina, Iowa. He earned a bachelor's in secondary education from Dana College in Blair, Nebraska, but then realized that teaching high school wasn't for him. Instead, he attended the University of Iowa, where he earned a master's and doctorate in political science. In 1981, he joined the University of Nebraska-Lincoln as an assistant professor of political science and rose quickly through the ranks. Initially, he studied the reasons that members of the U.S. Congress retire. This led to a longer-term collaboration with Kevin Smith and …

Diagnosis Of Covid-19 Impact On The Construction Employment: Decline And Recovery, Anil Baral, Abdullah Alnuaimi, Yunping Liang, Baabak Ashuri, Mohsen S. Shahandashti

Durham School of Architectural Engineering and Construction: Faculty Publications

The Coronavirus Disease 2019 (COVID-19) had an unprecedented impact on U.S. construction employment. The assessment of employment declines and recoveries across various construction sectors, workforce demographics, and geographic regions helps develop inclusive long-term plans to overcome the setbacks of natural hazards, such as the COVID-19 pandemic. Existing methods for quantifying the employment decline from sudden shocks do not consider trends and seasonality of construction employment under normal conditions. Hence, it is not clear whether the employment declines are due to the impact of a disaster or associated with trends and seasonal patterns of employment. The objective of this research is …

Statistical Methods In Research, Horahenage Dixon Vimalajeewa

UNL Faculty Course Portfolios

This course portfolio documents the design, delivery, assessment, student-learning evidence, and reflective evaluation of STAT 801A-700: Statistical Methods in Research, an online asynchronous service course designed to introduce students from diverse disciplinary backgrounds to foundational statistical reasoning and applied data analysis. The course is a non-calculus-based introduction to statistical methods used to answer research questions, with emphasis on collecting, organizing, describing, analyzing, and drawing conclusions from data. The course also emphasizes applications relevant to biology, agriculture, and other research-oriented fields. This is an online distance course aimed to develop students’ understanding of basic probability and statistical concepts, recognize the importance …

First Course Portfolio For Plpt 803 Fundamentals Of Scientific Discovery, Hernan Garcia-Ruiz

UNL Faculty Course Portfolios

PLPT 803 Fundamentals of Scientific discovery is a new curse in the Department of Plant Pathology at the University of Nebraska-Lincoln. I designed and developed this course from the ground up. Initially, it was offered as a special topic (PLPT 892-014) for four years. PLPT 803 was approved by the College of Agricultural Sciences and Natural Resources curriculum committee in September of 2025. PLPT 803 is unique within the University of Nebraska system and will be offered for the first time in the fall of 2027. The course is designed for students to learn fundamental scientific skills such as scrutinize, …

Shared Language For Responsible Ai Integration, Asa B. Stone, Mark C. Stone, Alisha Bevins, Jean Claude Niyomugabo, Irene Magara, Jacob Abaare, Derek M. Heeren, Mubarak Abu Zouriq

PRAIRIE: Pioneering Responsible AI for Research, Innovation, and Education

As AI rapidly reshapes how we work and learn, employers increasingly seek graduates who can think before they prompt, exercising judgment under pressure rather than merely producing output. Yet students are praised for AI use in one course and penalized for it in the next, and faculty are left to lead responsibly on shifting ground, with no shared language to guide them.

This paper introduces the PRAIRIE Framework for AI Integration, a shift from reactive gatekeeping toward proactive stewardship. It emerged from a qualitative sentiment analysis of three communities (students, faculty, and industry partners) whose concerns converged on one need: …

Thermodynamic Modeling Of Ph Impacts On Subsurface Microbial Consortia, Jackfin K C

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Underground hydrogen storage is affected by microbial consumption, and the different minerals present in the subsurface. However, most studies consider either biotic or abiotic factors in isolation and hence, the conditions that drive a self-sustaining storage remain poorly understood. This thesis uses a genome-informed thermodynamic model developed by Kharel et al. (2026) in which pH is the central variable coupling hydrogenotrophic microbial metabolism, inter-species competition, and carbonate mineral buffering. The model includes four hydrogen-consuming species: methanogen (Methanobacterium), acetogen (Acetoanaerobium), aerobic acetate oxidizer (Pseudomonas stutzeri), and sulfate reducer (Nitratidesulfovibrio) by conditioning each organism's …

Investigation Of Warfarin–Human Serum Albumin Binding By Ultrafast Affinity Extraction Using Single- And Dual-Column Affinity Microcolumn Systems, Samiul Alim

Department of Chemistry: Dissertations, Theses, and Student Research

This dissertation examined the interaction of warfarin with human serum albumin (HSA) by using affinity chromatography, with particular emphasis on ultrafast affinity extraction (UAE) and affinity microcolumns. HSA affinity supports were prepared by immobilizing HSA onto diol-bonded silica via Schiff base chemistry and used to prepare affinity microcolumns for chromatographic studies under physiological-like conditions at pH 7.4 and 37.0 °C. UAE studies were performed to investigate the effects of residence time, flow rate, and column configuration on the measured apparent free fraction of warfarin and on the calculated apparent binding parameters. The results showed that the measured apparent free fraction …

Can Generative Ai Make Farming Decisions? Current Status And Future Pathways: A Case Study In Row Crop Production With Chatgpt, Nipuna Chamara, Yufeng Ge, Joe Luck, Yu Pan, Saleh Taghvaeian, Cory Walters, Christopher Proctor, Daran Rudnick, Daren Redfearn

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The agricultural decision-making process is experience-based, knowledge-dependent, time-sensitive, complex, and driven by historical data. Planting, fertilization, irrigation, and chemigation are key categories in farm decision-making, and currently there is no one-shot decision-support tool that covers all these activities. Generative Artificial Intelligence (AI) models are more advanced than traditional machine learning and deep learning models. These models have been trained on vast amounts of data from the internet, allowing them to accept unstructured data in various forms and generate human-like text, solutions to problems, and scenario predictions. Given this capability, we became interested in exploring the potential of generative AI in …

Gobbling Activity And Use Of Structural Habitat At Nest Sites By Wild Turkeys (Meleagris Gallopavo) In Western Nebraska, Robyn M. Dausener

School of Natural Resources: Dissertations, Theses, and Student Research

Wild turkey (Meleagris gallopavo) populations in Nebraska have declined substantially in recent years. Understanding gobbling chronology and its environmental drivers may inform spring hunting season structure and management actions. I used autonomous recording units (ARUs) in two ecologically distinct regions of Nebraska, the northwestern (NW) Pine Ridge landscape, and the southwestern (SW) agricultural landscape, to quantify gobbling activity from 2023-2025. I paired gobbling data with nesting chronology, cumulative daily hunting pressure, weather conditions, and moon phase using Bayesian generalized additive mixed models. Gobbling peaked during mid-to-late April in both regions, preceding peak nest initiation and incubation, with no …

Increasing Students’ Self-Efficacy In Consuming, Conducting, And Disseminating Neuroimaging Research: Experiences From Teaching Edps 991: Social And Affective Neuroscience, Isabella Starling Alves

UNL Faculty Course Portfolios

Social and emotional factors are critical for learning, being core aspects of the educational  psychology curricula. Recently, neuroimaging has emerged as a powerful tool to  comprehend the neurobiological factors underlying these factors. Yet, many educational  psychologists have low familiarity with neuroscience research. The course EDPS 991:  Social and Affective Neuroscience, from the Department of Educational Psychology at the  University of Nebraska-Lincoln, emerges in this context, training educational psychology  graduate students to consume, conduct, and disseminate findings about how the brain  relates to socioemotional processes. One challenge related to the course is that many  students feel anxious about learning the neuroimaging …

Robert, Powers; Unl Chemistry; Nmr-Assisted Drug Discovery, Mark Griep, Robert Powers

Department of Chemistry: Faculty Interviews

Dr. Robert Powers became a chemistry professor at the University of Nebraska-Lincoln in 2003 and is about to retire after 23 years. Prior to UNL, Bob was a drug discovery researcher for 11 years at American Cyanamid, which eventually became Wyeth and is now part of Pfizer. Bob was born in Jersey City, New Jersey. Something in his youth must have sparked an interest in chemistry because he earned a bachelor's in that subject from Rutgers University in New Brunswick. Then he traveled 1200 miles west to Purdue University in Indiana where he earned his doctorate. Next, he did postdoctoral …

Recognition Of The Spring Flight Of Celastrina (Tutt) (Lycaenidae: Polyommatinae) In Southern New England As An Introgressed Hybrid Species, Harry Pavulaan

The Taxonomic Report of the International Lepidoptera Survey

Populations of Celastrina (“Azure”) butterflies in southern New England have historically been referred to as either C. ladon (Cramer) or C. lucia (W. Kirby) by various authors. Following the discovery of elongated dorsal wing scales in males of C. ladon (D. Wright & Pavulaan, 1999; Ômura et al., 2015)—structures that replace the androconia that are typically present in most Celastrina species—it was believed that this trait was restricted to C. ladon, C. nigra (W. Forbes), and, more recently, C. iryna (Pavulaan, 2025). Microscopic examination of male “Spring Azure” specimens from across southern New …