Digital Commons Network^™

Avian Influenza And The Approach To Designing An H5n1 Vaccine, Lauren N. Hickey

Honors Program: Senior Projects (Public)

The Avian influenza virus (AIV) H5N1 has been responsible for the culling and deaths of millions of birds since 2022 with concern for wildlife, livestock, and human infections occurring as well. With eggs flying off the shelves in grocery stores, even individuals not involved in the agricultural industry have seen the drastic effects of this disease first-hand. For chickens, this virus works quickly and overpowers the innate immune system before the adaptive immune system has time to properly respond. Therefore, a safe and effective vaccine could reduce the number of poultry deaths and prevent the continuous spread of the infection. …

Historical Chaos Among Tetrabothriideans (Eucestoda) Of Odontocetes (Artiodactyla)—Revising Generic Taxonomy And Species Diversity For Cestodes Among Oceanic Dolphins, Eric P. Hoberg, Kaylen Marie Soudachanh, Robert L. Rausch

MANTER: Journal of Parasite Biodiversity

Explorations over the past 150 years that have defined the diversity and structure of the tetrabothriidean cestode fauna among Odontocete cetaceans (Artiodactyla) are summarized and reviewed. Persistent errors in interpretation of comparative morphological characters, inconsistent taxonomic decisions, and an understanding of generic-level and species diversity are addressed. We outline a revision of the Tetrabothriidea, which now encompasses 8 genera. Prosthecocotyle is resurrected from synonymy with Tetrabothrius, and Cetaceobothridius n. gen is proposed. A clear basis is outlined for differentiation of generic and species diversity among Cetaceobothridius, Priapocephalus, Prosthecocotyle, Strobilocephalus, Tetrabothrius, and Trigonocotyle that constitute the tetrabothriidean fauna of odontocetes; species …

First Course Portfolio For Plpt 803 Fundamentals Of Scientific Discovery, Hernan Garcia-Ruiz

UNL Faculty Course Portfolios

PLPT 803 Fundamentals of Scientific discovery is a new curse in the Department of Plant Pathology at the University of Nebraska-Lincoln. I designed and developed this course from the ground up. Initially, it was offered as a special topic (PLPT 892-014) for four years. PLPT 803 was approved by the College of Agricultural Sciences and Natural Resources curriculum committee in September of 2025. PLPT 803 is unique within the University of Nebraska system and will be offered for the first time in the fall of 2027. The course is designed for students to learn fundamental scientific skills such as scrutinize, …

Integrating Growth Performance And Pathogen Detection To Investigate Disease Resilience In Commercial Swine, Emmalise Hope Marie Meyer

Department of Animal Science: Dissertations, Theses, and Student Research

Disease resilience is an important trait in commercial swine production, influencing both animal health and growth performance under natural disease challenge. The primary objective of this study was to integrate growth performance data with longitudinal pathogen detection to better understand factors contributing to disease resilience in commercial pigs. A population of 1,014 pigs across four batches was monitored from weaning through the grow-finish phase, with body weights collected to calculate average daily gain (ADG). Genome-wide association analyses were conducted using BayesC and BayesIM approaches to identify genomic regions associated with growth performance. Longitudinal pathogen surveillance was performed using pen-level oral …

Exploring The Biocultural Profile Of The Undocumented Populations Of The Sonoran Corridor: A Skeletal Assessment Of The Prevention Through Deterrence Strategy, Mary A. Vaughn

Department of Anthropology: Theses and Student Research

Over the last thirty years the mortality rate of the undocumented population at the US-Mexico border has risen, requiring methods distinguishing US-born individuals from immigrants to improve the likelihood of identification and repatriation through foreign consulates. Forensic anthropologists with the Pima County Office of the Medical Examiner (PCOME) have advocated for the use of the biocultural profile, combining the biological profile with factors including oral health, osteological stress, and personal effects. The biocultural profile serves two purposes, functioning both as a tool for identification and with which to document structural inequality within communities of origin.

This study examines changes in …

Program And Proceedings, The Nebraska Academy Of Sciences 1880–2026: 146th Anniversary Year, One Hundred-Thirty-Sixth Annual Meeting

Nebraska Academy of Sciences: Programs and Proceedings

Program and abstracts of the proceedings for the Nebraska Academy of Sciences 136th annual meeting, 2025

Sessions

Aeronautics and Space Science

Anthropology

Biological and Medical Sciences

Earth Sciences

Ecology, Sustainability, and Environmental Science

Physics and Engineering

General Poster Session

2026 Maiben Lecture: Shifting Extremes: Understanding Nebraska’s Changing Climate and Preparing for What Lies Ahead, Deborah Bathke

2026 Friends of Science Awards: Julie Shaffer and Irina Filina

2026 C. Bertrand and Marian Othmer Schultz Colleaguate Schoalrship Award: Piper Ryschon and Bryce Reeson

In Memoriam: Paul Royster

Metabolic Responses To Freund’S Adjuvants And Oncolytic Viruses: Implications On Vaccines And Tumor Immunity, Kiruthiga Mone

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Freund’s adjuvants have been extensively used in experimental research, and they modulate immune responses through various mechanisms. While both complete Freund’s adjuvant (CFA) and incomplete Freund’s adjuvant (IFA) contain the same mineral oil base, CFA contains the killed extract of Mycobacterium tuberculosis (M. tb) as an additional ingredient. Because the CFA contains mycobacterial content similar to that of Bacillus Calmette-Guérin (BCG), which induces trained immunity (TI), we tested the hypothesis that CFA may elicit TI-like features. To test this hypothesis, we investigated the metabolic and epigenetic reprogramming induced by CFA, with IFA and BCG as controls, in inbred …

The Social And Biological Frontiers Of Polio: Comparing Global Eradication Strategies, Srisai A. Turangi

Honors Program: Senior Projects (Public)

This paper provides a comparative analysis of the divergent global strategies for poliomyelitis (polio) eradication, examining the historical and structural contexts of developed nations – the United States and United Kingdom – alongside the enduring challenges in endemic regions such as Nigeria and Pakistan. Biomedical innovations like the Inactivated Polio Vaccine (IPV) and Oral Polio Vaccine (OPV) have helped bring the world to a state of almost complete global eradication, but progress is still uneven due to variations in funding, healthcare infrastructure, and the legacy of colonialism. Studies reveal how a framework where global health inequalities remained, allowed for resource-rich …

Review Of Vampire Forensics: Uncovering The Origins Of An Enduring Legend, Elizabeth Brandt

Nebraska Anthropologist

Review of Vampire Forensics: Uncovering the Origins of an Enduring Legend (2010) by Mark Collins Jenkins.

Large-Scale Experimental Study On The Impact Of Air Cleaning & Ventilation On Indoor Air Quality And Student Illness-Related Absenteeism, Daud Nosham

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This large-scale randomized experimental field study investigated the effect of portable air purifiers (PAPs) on classroom’s air quality and student’s illness related absenteeism. PAPs were installed in 317 classrooms from various school districts located in Eastern Nebraska. The classrooms were randomly assigned into four conditions: three treatment conditions (T1, T2 & T3) and one control condition (device without any filter). T1 was equipped with HEPA filters, T2 added Activated Carbon (AC) layer to HEPA, and T3 further integrated Germicidal Ultraviolet (GUV) alongside HEPA and AC filters. The measured variables were carbon dioxide (CO2), fine particles (PN2.5) and coarse particles (PNcoarse), …

Recovery And Characterization Of Moraxella Species From Bovine Specimens, Laura G. Leger

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

The family Moraxellaceae is made up of a diverse group of 24 named species that fall into three phylogenetic groups. While most Moraxella spp. act as commensal respiratory and/or ocular flora of mammals, some are associated with disease. One of these diseases, infectious bovine keratoconjunctivitis (IBK), has significant welfare and economic impacts to both cattle and cattle producers. Moraxella bovis is the only experimentally determined cause of IBK, however, an association of Moraxella bovoculi with disease has been proposed. Currently, available vaccines do not have high levels of efficacy in the field. Culture based methods are the gold standard for …

Flea News 96 (November 2025), R. L. Bossard

Flea News

Flea News is a twice-yearly newsletter about fleas (Siphonaptera). Before including in publications any of the citations given here, please review for accuracy and correct formatting. Many of our sources are abstracting journals and current literature sources, and editors have not necessarily checked citations.

Announcements

Obituary: Lane Douglas Foil (June 16, 1949 - June 22, 2025)

Siphonaptera Literature (2025): Taxonomy, Conservation, Hosts and Distributions, Physiology and Morphology, Evolution, Ecology, Pathology and Control, Microbial Symbionts

Use Of Modified Z-Domain Peptides To Specifically Label The Constant Region Of Immunoglobulin G And Vegf, Madeline Allen

Department of Chemistry: Dissertations, Theses, and Student Research

Antibodies are increasingly being used in medicinal therapies due to their ability to specifically bind to a target antigen. This fundamental behavior of antibodies has been used as a key component in drug development to address common problems with traditional drug molecules such as off-targeting, fast drug clearance, and low efficacy. This thesis provides some background into how antibodies can be used as potential treatments for a few diseases. Chapter 1 begins with an overview of the human immune system, and the role antibodies play within it. Then, the variety of ways in which antibodies can be used as medicinal …

Evaluation Of Performance Characteristics And Groundwater Contamination Risks Associated With On-Farm Swine Carcass Disposal Via Composting And Shallow Burial With Carbon, Gustavo Castro Garcia

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation evaluates the performance of three on-farm swine carcass disposal methods: whole carcass composting (WCC), ground carcass composting (GCC), and shallow burial with carbon (SBC), focusing on biosecurity factors and groundwater contamination risks. Foreign animal diseases (FADs), such as African swine fever and classical swine fever, pose severe economic and animal well-being risks if introduced to the United States. With confirmation of an FAD, swine movement will be halted, creating an urgent need for practical, biosecure, and environmentally responsible on-farm disposal strategies for swine carcasses. Nebraska, as a leading swine-producing state, exemplifies the vulnerability of high-density livestock regions to …

Flooding: Risk Assessment And Its Interaction With Population & Environmental Health, Shivendra Srivastava

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Flood events have significant implications for both population and environmental health. This dissertation aims to explore flood risk and its interaction with humans and the environment. The key questions addressed in this dissertation include improving the existing flood risk framework to better assess population vulnerability and associated risk. Additionally, it explores how interactions among risk components vary across spatial scales and regions, and how these variations influence overall flood risk. The dissertation also investigates how to design a vulnerability component specific to flooding within flood risk estimation. Finally, it answers whether flood-specific vulnerability differs for a population compared to vulnerability …

A New Species Of Viscachataenia (Cestoda: Anoplocephalidae) From The Southern Mountain Viscacha, Lagidium Viscacia (Hystricomorpha: Chinchillidae), In Bolivia, Terry R. Haverkost, Scott L. Gardner

MANTER: Journal of Parasite Biodiversity

Anoplocephalid cestodes collected from the southern mountain viscacha, Lagidium viscacia, in Boliva in 1986 are here examined, and a new species of Viscachataenia is described. The new species has numerous body measurements that are larger than the existing species, V. quadrata, but the eggs and corresponding structures of the new species are all smaller than those of V. quadrata.

The Effect Of Individual Variation On Epidemiological Dynamics: From Super-Spreaders To Landscapes Of Risk, Alexis Shannon Beagle

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Individuals that make up a population always display some level of variation between members. At the smallest scale, individuals always maintain genetic variation, but variation continues to arise at other scales of biological organization from physiological or immunological variation within individuals to variation in behavior between individuals that can be both genetically and environmentally mediated. The role of individual variation is particularly important in the context of infectious diseases because there is strong evidence that infections are highly heterogenous, indicating that patterns of individual variation drive a significant portion of the epidemiological patterns we observe. Individual variation leads to heterogeneity …

Cazyme Gene Cluster Diversity In Human Gut Microbiome, Yi Xing

Department of Food Science and Technology: Dissertations, Theses, and Student Research

In gut microbiome research, carbohydrate-active enzyme gene clusters (CGCs) have emerged as key functional units for understanding microbial glycan degradation. Unlike taxonomic or broad pathway annotations, CGCs offer gene-cluster-level resolution and capture substrate-specific microbial functions. However, their diversity and distribution in relation to host metabolic phenotypes, such as obesity, remain poorly characterized. This study tests the hypothesis that the composition and abundance of fiber-targeting CGCs vary between obese and healthy human gut microbiomes, reflecting distinct microbial carbohydrate utilization strategies. To examine this, we constructed a high-quality reference CGC dataset comprising 94,019 clusters from the Unified Human Gastrointestinal Genome and profiled …

Overcoming Maternal Antibody Interference In Swine Influenza: Advancing Dna Vaccine Strateies For Enhanced Immune Protection In Piglets, Danh Cong Lai

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Maternally derived antibodies (MDAs) play a vital role in protecting neonates from infectious diseases, but their presence at the time of vaccination can interfere with vaccine-induced immune responses, thereby reducing vaccine effectiveness. MDA interference has been documented in pigs vaccinated with whole inactivated virus (WIV) vaccines against swine influenza A virus (IAV). In this thesis, we evaluated the efficacy of a lipid nanoparticle encapsulated DNA (LNP-DNA) vaccine against swine IAV in the presence and absence of MDAs, comparing it to a WIV vaccine. In MDA-negative piglets, both the LNP-DNA and WIV vaccines induced strong immune responses and effectively prevented the …

Creation Of An Open Access Textbook, Sue Ann Gardner

University of Nebraska-Lincoln Libraries: Presentations

Slide presented during the PRCA Research Celebration, June 3, 2025, in the University Libraries, University of Nebraska-Lincoln titled Creation of an Open Access Textbook, about the development and publication of Concepts in Animal Parasitology, co-edited by Scott L. Gardner and Sue Ann Gardner and published by Zea Books (Lincoln, Nebraska, USA) in 2024.

Overcoming Vaccine-Associated Enhanced Respiratory Disease In Swine Flu With Lipid Nanoparticle-Dna Technology, The Nguyen

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

The two primary challenges in developing vaccines against swine influenza A virus (IAV-S) are the continuous viral evolution and vaccine-associated enhanced respiratory disease (VAERD). Current vaccine strategies share a common limitation: the significant time required for licensing updated vaccines. Moreover, the strain selection for vaccine updates depends entirely on predictions, raising the risk that chosen strains may not match circulating viruses. Such mismatches not only reduce vaccine efficacy but also risk exacerbating disease severity through VAERD. Here, in this thesis, we developed and evaluated a novel DNA-based vaccine platform designed to address the need for rapid updates in response to …

Investigating Fatty Acid Influence On West Nile Virus In Mosquitoes And Birds, Hayley Moree

Honors Program: Senior Projects (Public)

Vector-borne diseases, including Zika virus (ZIKV), Dengue virus (DENV), and West Nile virus (WNV), pose considerable global health threats, accounting for over 400 million infections and more than 700,000 deaths annually. Many vector-borne diseases are within the Flaviviridae family. Despite this, effective treatments have not been found for some flaviviruses. Flaviviruses exploit host cellular machinery to facilitate their replication and survival, reprogramming metabolic pathways, including lipid metabolism. Lipids play essential roles in the viral life cycle, providing structural components for the viral envelope, enabling membrane rearrangements for replication complexes, and supplying energy resources necessary for particle assembly.

Targeting lipid metabolism …

Novel Chloroviruses With Unique Glycans On Their Major Capsid Proteins, Cayden J. Homolka

Honors Program: Senior Projects (Public)

Chloroviruses, of the family Phycodnaviridae, are large, dsDNA viruses with a genome of 290 to 410 kb. These virus genomes are so large that they are classified as “giant viruses”. Within this genome, up to 16 tRNAs and 330 to 450 proteins are encoded. Among the massive amounts of genes and proteins are unique carbohydrate synthesis/metabolism genes, which are responsible for the glycosylation of chloroviruses major capsid proteins. In most viruses, these proteins are glycosylated by the host enzymes located in the endoplasmic reticulum. Instead, Chloroviruses encode their own glycosylation machinery; essentially decorating themselves. Furthermore, these chlorovirus glycans are unlike …

Epidemiological Inferences Of The Impacts Of Winter Ticks On Moose Health, Krishna S. Woerheide

Department of Entomology: Dissertations, Theses, and Student Research

The North American Moose (mooz in Anishinaabemowin, Ojibwe language, Alces alces), a culturally significant subsistence species for the Anishinaabe people, faces population decline due to calf predation, pathogen transmission, and parasitism by the winter tick (Dermacentor albipictus), all exacerbated by climate change. This study explores the impact of winter tick infestations on moose health, with a focus on natural mineral seeps as potential transmission hotspots in moose habitats within the Grand Portage Indian Reservation (Gichi Onigaming), on Isle Royale National Park (Minong), and within the 1854 Ceded Territory (greater northeastern Minnesota) - ancestral and present homelands of …

Parasitic Effects Of Amoebidium Appalachense On Daphnia Magna Heart Rate, Hopping Frequency, And Metabolic Rate, Vanessa Wise

Honors Program: Senior Projects (Public)

Daphnia magna are a crustacean that inhabits multiple different types of freshwater ecosystems and thus is plays a part in many different types of interspecies interactions, one of which is parasitism. Amoebidium appalachense is a Mesomycetozoean parasite of aquatic arthropods that colonizes the antenna of D. magna. Previous research has shown that A. appalachense infection will reduce the fecundity and increase mortality rate of D. magna but how it causes these effects is unknown. To determine if A. appalachense causes these effects on D. magna by either stealing nutrients inside the D. magna like a tick or by compromising …

Host Genetics Role In Viral Disease Susceptibility, Christian Willis Eaton

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Viruses and their hosts exist in an evolutionary arms race, with evolutionary promotion of host genetic-based defense mechanisms and evolution of virus genetic-based mechanisms to overcome host defenses. Viruses are capable of much more rapid evolution and adaptation than their hosts, often leading to the use of host cellular mechanisms to promote viral infection and replication. In this study, we describe the generation of a detection assay for atypical porcine pestivirus (APPV), designed to target the viral 5’ UTR, a region of the viral genome which sees less mutations than the protein coding regions. This assay was generated to be …

Cardiometabolic Benefits Of A Non-Industrialized-Type Diet Are Linked To Gut Microbiome Modulation, Fuyong Li, Anissa M. Armet, Katri Korpela, Junhong Liu, Rodrigo Margain Quevedo, Francesco Asnicar, Benjamin Seethaler, Tianna B. S. Rusnak, Janis L. Cole, Zhihong Zhang, Shuang Zhao, Adele Gagnon, Edward C. Deehan, Joao F. Mota, Jeffrey A. Bakal, Russell Greiner, Dan Knights, Nicola Segata, Stephan C. Bischoff, Laurie Mereu, Andrea M. Haqq, Catherine J. Field, Liang Li, Carla M. Prado, Jens Walter

Department of Food Science and Technology: Faculty Publications

Industrialization adversely affects the gut microbiome and predisposes individuals to chronic non-communicable diseases. We tested a microbiome restoration strategy comprising a diet that recapitulated key characteristics of non-industrialized dietary patterns (restore diet) and a bacterium rarely found in industrialized microbiomes (Limosilactobacillus reuteri) in a randomized controlled feeding trial in healthy Canadian adults. The restore diet, despite reducing gut microbiome diversity, enhanced the persistence of L. reuteri strain from rural Papua New Guinea (PB-W1) and redressed several microbiome features altered by industrialization. The diet also beneficially altered microbiota-derived plasma metabolites implicated in the etiology of chronic non-communicable diseases. Considerable …

The Importance Of Public Health In College Populations: Why Vaccines Are Important, Kashish Poore

Honors Program: Senior Projects (Public)

This paper examines the importance of vaccines in college populations, where high-density living environments create unique vulnerabilities to infectious disease outbreaks. Despite vaccine mandates at most institutions, research indicates that many college students possess incomplete understanding of vaccine mechanisms and importance. The paper addresses three interconnected aspects: the biological foundations of vaccine-induced immunity, the significance of vaccination in collegiate settings, and the current state of vaccine literacy among students. Historical outbreaks on campuses, including meningitis at Princeton and mumps across several institutions, highlight the consequences of undervaccination. Social media misinformation has contributed significantly to vaccine hesitancy, particularly since COVID-19. The …

Exploring The Pathogen Profiles Of Ancient Feces, Drew Capone, David Holcomb, Amanda Lai, Karl Reinhard, Joe Brown

Karl Reinhard Publications

Analysis of ancient desiccated feces – termed paleofeces or coprolites – can unlock insights into the lives of ancient people. We collected desiccated feces from caves in the Rio Zape Valley in Mexico (725-920 CE). First, we extracted DNA with methods previously optimized for paleofeces. Then, we applied highly sensitive modern molecular tools (i.e., PCR pre-amplification followed by multi-parallel qPCR) to assess the presence of 30 enteric pathogens. We detected ≥1 pathogen associated gene in each of the ten samples and a mean of 3.9 pathogens per sample. The targets detected included Blastocystis spp. (n=7), atypical enteropathogenic E. coli (n=7), …

Course Portfolio: Bios 443/843 Immunology, Nicole Sexton

UNL Faculty Course Portfolios

This course portfolio relates to the Fall 2025 delivery of BIOS 443/843: Immunology, cross listed as MBIO 443 and VMBS 843 and was prepared as part of the Faculty-led Inquiry into Reflective and Scholarly Teaching (FIRST) project. BIOS 443/843 is an upper division course that introduces Immunological concepts but also connects them to up to date literature in the field of Immunology with the intent of preparing students for careers in medicine and biomedical research. In Fall of 2025 this course was restructured to incorporate additional peer-to-peer learning opportunities and more distributed assessment styles, therefore was peer-reviewed to assess the …