Digital Commons Network^™

Physical Modeling To Represent Molten-Slag Entrapment Behavior Into Molten-Steel Under Simultaneous Similitude Condition Of Multiple Dimensionless Numbers, Yuichi Tsukaguchi, Sho Nakano

Progress in Scale Modeling, an International Journal

Physical model construction and experiment are essential for scientific investigations of flow phenomena such as high temperature two phase flow of molten metal and inert gas. It has been a common method to construct a physical model with a convenient scale then proceed experiments under similitude condition of one or two dimensionless number(s) of dominant forces to represent. It is an ideal experimental method to satisfy the simultaneous similitude condition of multiple dimensionless numbers concerned with the objective flow phenomena but has been considered difficult or impossible to realize for many years. Contrarily, the concept of the simultaneous similitude of …

2026 Safety Belt Usage Survey In Kentucky, Erin Lammers-Staats, Derek S. Young, Kenneth R. Agent

Kentucky Transportation Center Research Report

No abstract provided.

Kentucky Transportation Cabinet Secretary’S Office Of Safety ‘Safety Academy’, Gabriel Dadi, Farshid Taherpour, Amanda Casolare

Kentucky Transportation Center Research Report

The Kentucky Transportation Cabinet (KYTC) Safety Academy was developed to provide a structured and practical safety training program that strengthens safety leadership, planning, communication, employee engagement, hazard recognition, and continuous improvement across KYTC operations. The program was refined over the course of multiple discussions with Cabinet representatives to ensure that the content reflected the needs and responsibilities of KYTC personnel. The final Safety Academy consists of six modules: Safety Academy Welcome, Safety Leadership, Safety Planning, Safety Engagement, Hazard Recognition, and Safety Academy Closeout and Evaluation. The modules combine instructional content with discussions, practical examples, group exercises, and KYTC-related scenarios to …

Karst Hydrogeologic Framework And Factors Contributing To Flooding In Burgin, Kentucky, Charles Taylor, Junfeng Zhu, Matthew Carmichael, Hudson J. Koch

KGS Publications and Maps

This report examines the karst hydrogeologic setting and drainage characteristics that are thought to contribute to frequent severe flooding in the city of Burgin in Mercer County, Kentucky. Burgin is located at the eastern end of an approximately 4.5 mi² mapped karst groundwater basin in the Ordovician limestone aquifer system in the Inner Bluegrass karst region. Flooding occurs within a streamless 1.4 mi² dry karst valley where almost all channelized surface runoff has been captured by sinkholes and sinking stream segments and diverted underground by con­duit piracy. The only natural outlet for water exiting from the karst valley and larger …

Development Of Reduced Conflict U-Turn (Rcut) Guidance In Kentucky, Travis Thompson, Duane Thomas, Jill Asher, Jeff Jasper

Kentucky Transportation Center Research Report

A reduced conflict U-turn (RCUT) intersection eliminates left-turn and through movements from the minor road. Instead, drivers must turn right onto the main road and complete a U-turn at a downstream median opening. Recognized by the Federal Highway Administration (FHWA) as a proven safety countermeasure, RCUT intersections have reduced fatal and injury crashes 22 – 63%. Despite more than 35 RCUT intersections in operation across Kentucky, the Kentucky Transportation Cabinet (KYTC) lacked specific guidance for their planning, design, construction, and operation.

This project, conducted by the Kentucky Transportation Center (KTC) in cooperation with KYTC, addressed this gap through a comprehensive …

Cellular Senescence Links Muscle Atrophy And Posttraumatic Osteoarthritis After Acl Injury, Alexander R. Keeble, Allison Owen, Sara Gonzalez-Velez, Nicholas T. Thomas, Camille R. Brightwell, Peyton J. Balawender, Madeline G. O'Daniel, Helena C. Weiss, Haley Noehren, Heather Thompson, Karen Sofia Zuluaga-Osorio, Zane T. Bates, Lauren Gottlieb, Douglas E. Long, Douglas Harrison, Philip Kern, Caitlin E. Conley, Michael A. Samaan, Stephen Duncan, Austin Stone, Darren Johnson, Yuan Wen, Brian Noehren, Cory Dungan, Christopher Fry

Biology Faculty Publications

Traumatic knee injury leads to posttraumatic osteoarthritis (PTOA) and significant skeletal muscle weakness, resulting in chronic disability. The current standard of care frequently fails to prevent musculoskeletal dysfunction, underscoring the need to identify therapeutic mechanisms of PTOA. Using an established preclinical anterior cruciate ligament (ACL) transection model of PTOA and leveraging an innovative SPiDER-senescence-associated β-galactosidase stain to discern senescent cells, we investigated cellular senescence at single-cell resolution and identified anti-inflammatory macrophages as a predominant contributor to the senescent cell burden in both muscle and knee joint after injury. Clearance of senescent cells using the senolytic dasatinib and quercetin (D + …

Chameleon Carriers In Trucking: Detection, Enforcement, And Financial And Safety Impacts, Brian Howell, Drew Law, Andrew Martin

Kentucky Transportation Center Research Report

Chameleon carriers are motor carriers that evade regulatory enforcement by dissolving and reregistering under new corporate identities. This study, commissioned by the Kentucky Transportation Cabinet (KYTC), examined how states characterize, detect, and respond to chameleon carrier activity and assessed its financial and safety impacts in Kentucky from 2020 through 2025. The research team conducted a literature review of existing agency practices and relevant stakeholder perspectives, reviewed administrative codes and statutes across 13 pilot states, and administered two structured surveys to state motor carrier credentialing officials examining carrier characterization and enforcement practices. An analysis of KYTC's carrier data flagged by CVIEW …

Evaluation Of Kentucky’S Work Zone Speed Camera Pilot Program: Speed And Crash Analyses, Xu Zhang, William Staats, Eric Green

Kentucky Transportation Center Research Report

Kentucky introduced automated speed enforcement in work zones to mitigate hazards and improve the safety of motorists and workers. This study describes the impacts of automated speed enforcement at five pilot sites. Researchers analyzed speed data and crash data from pilot sites and paired control sites. The control sites and pilot sites had similar traffic volumes, lane configurations, and functional classifications. Analysis drew on over 15 million individual speed records captured using automated enforcement cameras as well as segment-level speed data from HERE.  Active enforcement at pilot sites was associated with lower mean, 85th-percentile, and 99th-percentile vehicle speeds. Average vehicle …

Kytc’S Digital Project Delivery Phase 2 Summary, Catherine Keathley

Kentucky Transportation Center Research Report

Begun in 2020, the Kentucky Transportation Cabinet’s (KYTC) Digital Project Delivery (DPD) Initiative is working to expand the use of digitally formatted plan and project information throughout transportation asset life cycles. A primary goal of the initiative is to provide users with access to accurate data on design intent, as-built records, and maintenance operations in a connected data environment. This report reviews work completed during Phase 2 of the initiative. Thirteen pilot projects are ongoing or have been completed. In response to the initiative’s growth, the Division of Professional Services no longer issues formal requests for proposals to solicit pilot …

New Procedures Of Preserving Beet Tops, Hedvika Maskova, Jiri Havelik

IGC Proceedings (1977-2023)

The possibility was tested of preserving chopped (homogenized) beet tops in experimental tower silos. The treatments without preservatives were compared with treatments using pro­pionic acid (0.5 %) and allylisothiocyanate (1: 10,000). Silage quality, dry matter losses, and secondary fermentation 24, 72, 168, and 240 hours after unloading were determined. Chopping of beet tops was found to have a positive effect on the utilization of the silo volume and on the mechanization of silage handling, and to avoid the need of supple­mental compaction. Allylisothiocyanate and propionic acid had a favourable effect on the preservation process and helped to cut losses caused …

A Complex System Of Grass Seed Drying And Cleaning, Hermann Pohler

IGC Proceedings (1977-2023)

Close socialist team-work between science and practice has allowed to develop a complex technology for grass seed handling by which raw seeds of up to 45 % moisture content, as received from the combine harvester, are dried and cleaned. The raw seeds are stored in a fully mechanized, ventilated section for pre-drying. Then, the seed crop is pre-cleaned, dried, and finally cleaned to be ready for dispatch, these operations being done in a continuous process. Apart from grass seeds, other small-seeded crops and coarse-seeded legumes can be handled by this plant. For coarse-seeded legumes it is especially the short distance …

[5,5]-Fused Anhydride, Thioanhydride, And Imide Derivatives Of Cyclopentadienyl Complexes, Uttam R. Pokharel, Sean Parkin, John P. Selegue

Chemistry Faculty Publications

A new approach to 5,5-fused heterocyclic derivatives of cyclopentadienylmanganese tricarbonyl and pentamethylruthenocene is presented. 1,2-Dicarbophenoxycyclopentadienyl complexes of manganese and ruthenium were hydrolyzed to 1,2-dicarboxylic acids. Oxalyl chloride converted the acids to chlorocarbonyls, which reacted with bis(trimethylsilyl)sulfide to give the cyclopentadienyl-fused thioanhydrides. Alternatively, dehydration of the diacids with trifluoroacetic anhydride closed the diacids to cyclopentadienyl-fused anhydrides. Treatment of the anhydrides with p-toluidine followed by oxalyl chloride led to cyclopentadienyl-fused carboxylic imides. This approach enables direct comparison of electron-deficient Mn(CO)₃ and electron-rich Ru(Cp*) coordination environments on the 5,5-fused heterocycles. Spectroscopic data reveal systematic downfield NMR shifts and higher infrared carbonyl stretching …

Elemental Analysis Of Upper Devonian To Lower Mississippian Organic-Rich Mudstone Cores From Kentucky By Portable X-Ray Fluorescence (Pxrf), Alex M. Washburn

KGS Research Data Set

This dataset contains high-density portable X-ray fluorescence (pXRF) elemental analyses of 1,867 m (6,125 ft) of Upper Devonian to Lower Mississippian organic-rich mudstone cores from Kentucky. Analyses were collected from cores across the Illinois Basin, Cincinnati Arch, and Appalachian Basin to support regional evaluation of elemental variability, chemostratigraphy, and redox-sensitive trace-metal enrichment in Devonian–Mississippian mudstone successions. All measurements were conducted using a Bruker Tracer 5 pXRF analyzer with the factory “Mudrock Air” calibration, using analytical settings optimized for both low-atomic-number elements and transition metals/redox-sensitive trace elements. Reference materials SARM-41 and SGR-1 were analyzed throughout the analytical sequence to monitor instrument …

Comparison Of The 1c2 Reference Filtered Cigar To The 1r6f Reference Filtered Cigarette, Amrita Machwe, Samuel B. Clark, Halle Harned, Tanvi Sawardekar, Stacey A. Slone, Huihua Ji, David K. Orren

Markey Cancer Center Faculty Publications

Introduction: Filtered cigars are a class of tobacco products that can be consumed similarly as conventional cigarettes. Here we have compared tobacco smoke condensates prepared from the 1C2 reference filtered cigar with those from the 1R6F reference cigarette with respect to effects on cell proliferation and viability and AhR-mediated gene expression. Methods: Tobacco smoke condensates were prepared using ISO, Health Canada Intense (HCI) or Cigar Smoking (CSR) regimens and certain HPHCs were measured. Cell proliferation and viability assays were performed on immortalized human bronchial or oral epithelial cell lines. AHR-mediated gene expression was measured using a mouse hepatoma cell line …

Three Centuries Of Technological Innovation, Jordan G. Okie, James H. Brown, Astrid Kodric-Brown, Joseph R. Burger, Tatiana P. Flanagan, Trevor S. Fristoe, Sean T. Hammond, Norman Mercado-Silva, Jeffrey C. Nekola, Jennifer Richter

Biology Faculty Publications

What socioecological conditions nurture the ingenuity and collaborative interactions underlying transformative technological innovations? We compiled a dataset on more than 400 major technological inventions from 1690 to 1990 spanning seven categories (agriculture, armaments, information and communication, household, industry, medical, and transportation) and performed inductive macroecological analyses to address how attributes of inventors, teams, and their social and geographic environments contributed to the innovation of new technologies that have changed the way people live. The vast majority of inventions were attributed to single inventors of various ages. The frequency, size, and cultural diversity of teams increased in the 20th Century, as …

Soft Korsi: An Embodied Spatial Interior System For Communal Warmth, Faezeh Panahandeh

Graduate Final Projects

ABSTRACT

Soft Korsi: An Embodied Spatial Interior System for Communal Warmth reconceptualizes the historic Iranian Korsi as an innovative textile-based interior system. Traditionally, the Korsi functioned not only as a heating device but also as a spatial, material, cultural, and embodied system, which brought people together around a shared warm focal point. The integration of a heat source, textile coverings, floor seating arrangements, closeness of bodies, and domestic traditions related to warmth made possible a collective experience, rather than an individual or environmental one.

This design project seeks to address the disappearance of the Korsi from contemporary domestic environments as …

Alternative Treatment Systems To Increase The Drying Rates Of Green Forage Crops, W E. Klinner, O D. Hale

IGC Proceedings (1977-2023)

Forms of conditioning treatment are being developed that aim to minimize the barrier effect of the crop cuticle without sacrificing too much of the structural strength of the herbage. Effective surface abrasion and favorable windrow structure can be achieved with steel-spoked rotors, which are now beginning to be offered commercially. More recently, plastic brushes have been developed because the higher population density and yielding ability of the crop-engaging elements, plus the absence of metal components, appeared to offer additional advantages. Four different brush conditioner systems have been evaluated in the laboratory and in replicated field experiments. Tufted brushes using trilobar …

Maximizing Room-Temperature Red Phosphorescence In Contorted Hexabenzocoronene Derivatives, Marko R. Ivancevic, Moses D. Ogbaje, Jesse A. Wisch, Daniel G. Oblinsky, Alice S. Fergerson, Emily C. Davidson, Gregory D. Scholes, Barry P. Rand, Chad Risko, Quinn C. Burlingame, Yueh Lin Loo

Chemistry Faculty Publications

Organic molecules exhibiting second-scale room-temperature phosphorescence (RTP) in the red/near-infrared are particularly rare because low-energy excited states that are characteristic of these chromophores are susceptible to nonradiative deactivation. Here, we observe second-scale red RTP from contorted hexabenzocoronene (cHBC) embedded in a rigid polymer. This RTP is uniquely efficient as approximately 23% of the steady-state photoluminescence originates from triplets. We propose that this efficient triplet generation stems from intersystem crossing (ISC) that outcompetes symmetry-forbidden fluorescence. Density functional theory and time-dependent density functional theory calculations suggest that ISC occurs from the lowest energy singlet state of cHBC into a nearly resonant triplet …

Endoplasmic Reticulum-Resident Α-Glucosidase Ii Drives Non-Small Cell Lung Cancer Progression Via Regulation Of Secretory Glycoproteins, Shike Wang, Na Ding, Angelo Chen, Derrick Cardin, Yuting Xu, Kate Grimley, William K. Russell, Jun Xu, Jonathan M. Kurie, Guan Yu Xiao, Xiaochao Tan

Markey Cancer Center Faculty Publications

Non-small cell lung cancer (NSCLC) remains a leading cause of cancer-related mortality worldwide, yet its molecular drivers are not fully defined. Emerging evidence highlights the importance of tumor-stroma interactions mediated by secreted glycoproteins. However, the mechanisms by which cancer cells regulate the secretion of these protumorigenic proteins remain largely unknown. Endoplasmic reticulum-resident (ER-resident) N-glycan-processing enzymes regulate proper protein folding, a prerequisite for glycoproteins to exit the ER and undergo secretion. By evaluating their prognostic significance in lung tumors and conducting functional screening in lung cancer cells, we identify α-glucosidase II (α-Glc II) as a key regulator of NSCLC progression. α-Glc …

Ultracold Neutron Energy Spectrum And Storage Properties From Magnetically Induced Spin Depolarization, N. J. Ayres, G. Ban, G. Bison, K. Bodek, V. Bondar, T. Bouillaud, D. Bowles, G. L. Caratsch, E. Chanel, W. Chen, P. J. Chiu, C. B. Crawford, V. Czamler, M. Daum, C. B. Doorenbos, M. Ferry, M. Fertl, A. Fratangelo, D. Galbinski, W. C. Griffith, Z. D. Grujic, K. Kirch, V. Kletzl, B. Lauss, T. Lefort, A. Lejuez, R. Li, K. Michielsen, J. Micko, P. Mullan, A. Mullins

Physics and Astronomy Faculty Publications

We present a novel method for extracting the energy spectrum of ultracold neutrons from magnetically induced spin depolarization measurements using the n2EDM apparatus. This method is also used to exploit sensitivity to the storage properties of the materials used to trap ultracold neutrons, specifically, whether collisions are specular or diffuse. To do so, we utilize tomat simulation to map storage properties to correlation times of the ultracold neutrons to induced magnetic nonuniformities. We highlight the sensitivity of this new technique by comparing the two different storage chambers of the n2EDM experiment, which host different ultracold neutron energy spectra. This method …

Tailoring Crystallization Kinetics For Scalable And Efficient Large-Area Perovskite Light-Emitting Diodes, Sung Doo Baek, Yuanhao Tang, Hyuntae Choi, Shang Jiang, Qixuan Hu, Yu Ting Yang, Hanjun Yang, Wenzhan Xu, Kyu Yoon, Devansh Drolia, Limei Wang, Syed Joy, Su Hye Jeong, Gangsan Lee, Vivek Narsimhan, Kenneth R. Graham, Jeffrey T. Miller, Jianguo Mei, Yoon Ho Lee, Letian Dou

Chemistry Faculty Publications

Scalable fabrication of uniform perovskite films is a critical bottleneck for large-area perovskite light-emitting diodes (PeLEDs), hindered by coffee-ring formation and heterogeneous crystallization upon scaling. Here, we report a multimodal solvent-engineering strategy enabling highly uniform films via ambient blade coating combined with vacuum-assisted solvent evaporation. Incorporating N-methyl-2-pyrrolidone (NMP) and acetonitrile (ACN) into a dimethylformamide (DMF)-based system synergistically modulates evaporation dynamics: It suppresses macroscopic solute segregation through Marangoni flow-induced redistribution, while tuning precursor coordination to regulate nucleation/phase conversion and promote radiatively efficient film formation. Consequently, the ternary formulation yields films with improved uniformity, reduced trap densities, and enhanced radiative efficiency. Near-infrared …

Research And Assessment Of Needs For Sign And Signal Maintenance, Megan White, Eric Green

Kentucky Transportation Center Research Report

Kentucky Transportation Cabinet (KYTC) sign and signal crews have expressed multiple concerns related to compensation, expertise, and having the financial and personnel resources needed to help the agency comply with Manual on Uniform Traffic Control Devices (MUTCD) guidelines while continuing to perform routine job duties. Internal reorganizations and personnel changes have reduced staffing and budgets available to underwrite the activities of sign and signal crews even as workloads have remained steady or increased. To document the challenges facing sign and signal crews and associated engineering staff, Kentucky Transportation Center (KTC) researchers administered a statewide survey. Respondents gave their perspectives on …

Building And Facility Asset Inventory Assessment And Maintenance, Catherine Keathley, Bethany Paris, Jon Wilcoxson

Kentucky Transportation Center Research Report

The Kentucky Transportation Cabinet (KYTC) manages approximately 1,500 buildings statewide, including facilities such as office buildings, maintenance and equipment garages, and storage sheds. Because managing buildings and properties requires considerable effort, agency decision makers need reliable monitoring and management tools that generate and organize data which can be used to determine the best course of action for facility maintenance and replacement. In addition to assessing the Cabinet’s building inventory, this report advances recommendations to improve the collection, management, and reporting of facility-related data. Assessment of the inventory managed by KYTC included geolocation of all properties and the buildings therein. Once …

Kytc Crash Modification Factor Recommendation List Online Tool, William Staats, Reginald Souleyrette, Teng Alex Wang, Mohammad Zana Majidi

Kentucky Transportation Center Research Report

Transportation practitioners use crash modification factors/functions (CMFs) to calculate the number of crashes expected once a countermeasure has been implemented. When evaluating design alternatives, CMFs can be used in conjunction with safety performance functions (SPFs) to derive crash predictions. The Federal Highway Administration (FHWA) maintains the CMF Clearinghouse as a repository for CMFs. Contributions are submitted by researchers across the U.S., Canada, and throughout the world. Typically, individual countermeasures are associated with multiple CMFs. Some CMFs only apply to a specific facility or crash types. CMF quality varies, and some only apply to a specific facility and/or crash types, regions, …

Development Of Kytc’S Context Classification Network, Jill Asher, Megan White, Eric Green, Christopher Van Dyke

Kentucky Transportation Center Research Report

Transportation agencies have adopted context-based classification systems that classify roadways based on the surrounding environment and user types. This offers a more comprehensive and multimodal perspective than traditional functional classification. While the Kentucky Transportation Cabinet (KYTC) has begun using project-level context classification, the agency will benefit from introducing network-level context classification. This will improve guidance, enhance project prioritization, and support the development of projects that better address the needs of all users. Responding to this need, Kentucky Transportation Center (KTC) researchers, in conjunction with KYTC staff, created a GIS-based model to build a context classification network that covers all state-maintained …

Agn Storm 2. Xii. Ground-Based Optical Photometry And Lag Measurements Of Mrk 817, John W. Montano, Aaron J. Barth, Keith Horne, Edward M. Cackett, Gisella De Rosa, Y. Homayouni, Erin A. Kara, Gerard A. Kriss, Hermine Landt, Gilvan G. Apolonio, Nahum Arav, Benjamin D. Boizelle, Elena Dalla Bontà, Doron Chelouche, Maryam Dehghanian, Rick Edelson, Gary J. Ferland, Carina Fian, Catalina Sobrino Figaredo, Michael R. Goad, Diego H. Gonzalez-Buitrago, Wei Jian Guo, Chen Hu, Dragana Ilić, Michael D. Joner, Shai Kaspi, Christopher S. Kochanek, Andjelka B. Kovačević, Collin Lewin, Sha Sha Li, Yan Rong Li, Jun Rong Liu

Physics and Astronomy Faculty Publications

We present the ground-based imaging campaign and light curves of Markarian 817 as part of the multiwavelength monitoring program AGN STORM 2. Observations were carried out over 1.4 yr in the uBgVriz filters, with a median cadence of 0.4 day in the g band. Reverberation lags are measured using three methods (interpolated cross-correlation function (ICCF), Just Another Vehicle for Estimating Lags In Nuclei, and PyROA) with the Swift UVW2 band (1928 Å) as the reference light curve. The ICCF centroid lags range from 3.0 ± 0.8 days for the u band up to 7.9 ± 1.5 days for z, and …

Thermodynamics Of Binding Between Adeno-Associated Viruses And Heparin In Bulk And At Interfaces Via Isothermal Titration Calorimetry, Elizabeth Adeogun, Jude C. Obijiaku, Ronny Horax, Kayla E. Daugherty, Joshua Sakon, Xianghong Qian, Barbara Knutson, Stephen E. Rankin, Karthik Nayani

Chemical and Materials Engineering Faculty Publications

Adeno-associated viruses (AAVs) have emerged as promising vectors for gene therapy due to their non-pathogenic nature and ability to transduce various cell types efficiently. In recent years, there has been an increasing effort to optimize the production and purification of AAV to support clinical applications; however, challenges exist in affinity ligand design, synthesis, and characterization. Understanding the binding interactions of these viruses with functional molecules is pivotal for the development of affinity-based separation methods of AAVs. Classical methods to measure thermodynamic parameters such as Isothermal Titration Calorimetry (ITC) are challenging to employ in these scenarios, as the concentrations of the …

Chemical Structure Representation Standardization Is Needed To Generalize Metabolite-Pathway Involvement Prediction Across Kegg, Reactome, And Metacyc Knowledgebases, Erik D. Huckvale, Hunter N.B. Moseley

Markey Cancer Center Faculty Publications

Background/Objectives: Due to the utility of knowing the pathway involvement of metabolites detected in biological experiments, knowledgebases such as the Kyoto Encyclopedia of Genes and Genomes (KEGG), Reactome, and MetaCyc have annotated compound entries to specific pathways defined by the knowledgebase. However, these compound-pathway annotations are largely incomplete and are costly to obtain experimentally or curate from published scientific literature. This metabolite-pathway annotation incompleteness problem is amenable to machine learning (ML)-based solutions. But to date, no machine learning model has been trained on all three knowledgebases to maximize its performance and robustness. This may be due to inconsistencies in chemical …

In Situ Molecular Organization And Heterogeneity Of The Legionella Dot/Icm T4ss, Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen

Markey Cancer Center Faculty Publications

The Dot/Icm type IV secretion system (T4SS) is essential for Legionella pneumophila infection, but its in situ architecture and mechanism remain incompletely understood. Using cryo-electron tomography, we performed subtomogram averaging and 3D classification to resolve structural heterogeneity within the complex. We identified multiple assembly states of the inner membrane complex, including a fully assembled form with a hexamer-of-dimers DotO ATPase and symmetry mismatches between subcomplexes. A composite in situ model revealed a central channel above the inner membrane, likely used for substrate secretion. Imaging of infected macrophages showed T4SSs tethered to host vacuoles and extracellular vesicle release, suggesting additional effector …

Brine Solidification And Metals Sequestration Using Lignocellulosics, Frank Kvietok

World of Coal Ash Proceedings

Coal ash has historically been used as a convenient, on-site solidification material for brines generated from flue gas desulfurization at power plants. As alternate utilization scenarios are developed for coal ash, lignocellulosic (wood-based) sorbents can serve as a highly efficient substitute for the solidification process. Results will be shared for a variety of different brine types: thermally reduced, thermally reduced and neutralized, and reverse osmosis. In addition to solidification performance, which demonstrated a 3-4x mass of liquid vs mass of sorbent factor, studies were conducted to quantify the strength of binding to the wood-based sorbent for a broad range of …