Digital Commons Network^™

Improving Diabetes Self-Management Through A Partnership Between Clinical Providers And Community Educators In Appalachian Communities, Soghra Jarvandi, Lydia Hoskins, Karen Carver, Andrew May

Journal of Appalachian Health

Introduction: The burden of diabetes is an increasing public health challenge, especially in the Appalachian Region.

Purpose: This study assessed the impact of a community-based diabetes education program on glycemic control.

Methods: Health Extension for Diabetes (HED) is a 4-month diabetes education program offered by Extension educators in collaboration with clinical providers. Participants’ data were collected via phone interviews at baseline and after completing the program. Assessments included self-reported HbA1c, socio-demographics, diabetes knowledge, self-efficacy, and self-management behaviors. Data were analyzed by SAS software using paired analyses.

Results: Of the 105 participants in the program, 87 (83%) completed the post-assessment. Among …

Major League Baseball's Major Labor Boondoggle: Rethinking Salary Arbitration, Matthew W. Givens

Kentucky Law Journal

No abstract provided.

The Separation Of Election Contest Powers, Casey Parker-Bell

Kentucky Law Journal

No abstract provided.

Immigration Detainers, Local Law Enforcement, And Constraints On Bureaucratic Discretion: Recent Trends Under The Second Trump Administration, Richard T. Middleton, Sheridan Wigginton

Kentucky Law Journal

No abstract provided.

From Militarization To Military Enforcement: The U.S. Armed Forces And Immigration Control In The Second Trump Administration, Kit Johnson

Kentucky Law Journal

No abstract provided.

The Duty To Develop Imperative In Immigration Proceedings, Sabrineh Ardalan, Tiffany J. Lieu

Kentucky Law Journal

No abstract provided.

Deportation After Death By Incarceration: A Right To Redemption, Jennifer Lee Koh, Tin Nguyen

Kentucky Law Journal

No abstract provided.

A Sherbert Colored Lens: Apache Stronghold And The Uphill Battle For The Recognition Of A Substantial Burden For Indigenous Plaintiffs, Emma P. Collins

Kentucky Law Journal

No abstract provided.

Hope In The Holler: How Eminent Domain Can Bring Economic Justice To Central Appalachia, Matthew B. Chaney

Kentucky Law Journal

No abstract provided.

Consumer Bankruptcy: A "Gem" Of The Legal Profession But A Diamond In The Rough, Tori Harris

Kentucky Law Journal

No abstract provided.

Arbitration Clause Expansion, Hossein Fazilatfar

Kentucky Law Journal

No abstract provided.

The New Parens Patriae, Meredith Johnson Harbach

Kentucky Law Journal

No abstract provided.

Cellular Senescence Links Muscle Atrophy And Posttraumatic Osteoarthritis After Acl Injury, Alexander R. Keeble, Allison Owen, Sara Gonzalez-Velez, Nicholas T. Thomas, Camille R. Brightwell, Peyton J. Balawender, Madeline G. O'Daniel, Helena C. Weiss, Haley Noehren, Heather Thompson, Karen Sofia Zuluaga-Osorio, Zane T. Bates, Lauren Gottlieb, Douglas E. Long, Douglas Harrison, Philip Kern, Caitlin E. Conley, Michael A. Samaan, Stephen Duncan, Austin Stone, Darren Johnson, Yuan Wen, Brian Noehren, Cory Dungan, Christopher Fry

Biology Faculty Publications

Traumatic knee injury leads to posttraumatic osteoarthritis (PTOA) and significant skeletal muscle weakness, resulting in chronic disability. The current standard of care frequently fails to prevent musculoskeletal dysfunction, underscoring the need to identify therapeutic mechanisms of PTOA. Using an established preclinical anterior cruciate ligament (ACL) transection model of PTOA and leveraging an innovative SPiDER-senescence-associated β-galactosidase stain to discern senescent cells, we investigated cellular senescence at single-cell resolution and identified anti-inflammatory macrophages as a predominant contributor to the senescent cell burden in both muscle and knee joint after injury. Clearance of senescent cells using the senolytic dasatinib and quercetin (D + …

Experimental Results With Various Herbicides In Established Alfalfa, Taib Saric, Refik Numic

IGC Proceedings (1977-2023)

5-year experiments with herbicides were run in the mixture of alfalfa, red clover, timothy grass, and Italian ryegrass. On untreated plots, in the 5-year average 72 weed plants were found per 1 m2 , belonging to 66 weed species. Among them most numerous were: Stellaria media, Veronica agrestis, Poa annua, Lamium purpureum,Taraxacum officinale, and Capsella bursa pastoris. The most efficient herbicide was Etazin, killing 88 % of weeds. None of the applied herbicides controlled perennial weeds. Etazin was tolerated by alfalfa, but was toxic to the other three crops. Kerb, Kerb-Mix, and Multi­tok were toxic to sown grasses, while …

Recommended Values For Dimensioning And Designing Sprinkler Irrigation In Industrialized Forage Plant Produc­Tion In The Gdr, Klaus Schwarz, Dieter Roth

IGC Proceedings (1977-2023)

With the transition to industrialized crop and animal production in the GDR, sprinkler irrigation of field forage plants and grassland, above all with sewage water and clean water +slurry mixtures, is becoming more and more important. It gets increasingly concentrated on field grass and grass/clover stands. The supple­mental water requirement is 210 to 270 mm/ year in case of clean-water sprinkling and up to 400 mm/year if sewage water is used. The hydro-modulus of the irrigation plants is be­tween 1.2 and 1.6 m3 /h/ha, depending on the simultaneity factor. In order to attain the required water quantity per unit of …

Comparison Of The 1c2 Reference Filtered Cigar To The 1r6f Reference Filtered Cigarette, Amrita Machwe, Samuel B. Clark, Halle Harned, Tanvi Sawardekar, Stacey A. Slone, Huihua Ji, David K. Orren

Markey Cancer Center Faculty Publications

Introduction: Filtered cigars are a class of tobacco products that can be consumed similarly as conventional cigarettes. Here we have compared tobacco smoke condensates prepared from the 1C2 reference filtered cigar with those from the 1R6F reference cigarette with respect to effects on cell proliferation and viability and AhR-mediated gene expression. Methods: Tobacco smoke condensates were prepared using ISO, Health Canada Intense (HCI) or Cigar Smoking (CSR) regimens and certain HPHCs were measured. Cell proliferation and viability assays were performed on immortalized human bronchial or oral epithelial cell lines. AHR-mediated gene expression was measured using a mouse hepatoma cell line …

Structural Characteristics Limiting Digestion Of Forage Fiber, D E. Akin

IGC Proceedings (1977-2023)

Cell walls of forage were studied for structural and chemical factors that limit digestion by rumen microorganisms. Tech­niques used to investigate digestion were histochemistry, electron microscopy, and anaerobic culturing of rumen populations on specific substrates. Certain tissues, such as the xylem cells, totally resisted degradation by rumen microorganisms; these tissues stained positive for lignin with acid phloroglucinol (Weisner test). Other supportive tissues, such as the sclerenchyma in blades, stained positive for lignin with chlorine-sulfite (and also the Maule test) and were also generally resistant to microbial digestion. Microscopic studies revealed that certain living tissues (i.e., those having cytoplasm and organelles …

Endoplasmic Reticulum-Resident Α-Glucosidase Ii Drives Non-Small Cell Lung Cancer Progression Via Regulation Of Secretory Glycoproteins, Shike Wang, Na Ding, Angelo Chen, Derrick Cardin, Yuting Xu, Kate Grimley, William K. Russell, Jun Xu, Jonathan M. Kurie, Guan Yu Xiao, Xiaochao Tan

Markey Cancer Center Faculty Publications

Non-small cell lung cancer (NSCLC) remains a leading cause of cancer-related mortality worldwide, yet its molecular drivers are not fully defined. Emerging evidence highlights the importance of tumor-stroma interactions mediated by secreted glycoproteins. However, the mechanisms by which cancer cells regulate the secretion of these protumorigenic proteins remain largely unknown. Endoplasmic reticulum-resident (ER-resident) N-glycan-processing enzymes regulate proper protein folding, a prerequisite for glycoproteins to exit the ER and undergo secretion. By evaluating their prognostic significance in lung tumors and conducting functional screening in lung cancer cells, we identify α-glucosidase II (α-Glc II) as a key regulator of NSCLC progression. α-Glc …

Antioxidant Therapy Reverses Hepatotoxicity Induced By Microcystin-Lr In A Cellular Model Of Metabolic Dysfunction-Associated Steatotic Liver Disease (Masld), Apurva Lad, Jason Kindle, Prajwal Hegde, Gabriel G. Kleer, Andrew L. Kleinhenz, Johnna A. Birbeck, Judy Westrick, Nicholas J. Peraino, Terry D. Hinds, Neeraja Purandare, Andrew M. Fribley, Steven T. Haller, David J. Kennedy

Markey Cancer Center Faculty Publications

Microcystin-LR (MC-LR) is a potent hepatotoxin that has been shown to cause liver damage even at doses lower than the established Low Observable Adverse Effect Level (LOAEL) of 200 μg/kg in animal models. We have previously observed that low-dose exposure to MC-LR in animals with diet-induced Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) and subsequent treatment with antioxidants like N-acetylcysteine (NAC) and the Na⁺/K⁺ ATPase-Src kinase inhibitor pNaKtide significantly alleviated hepatic infiltration of immune cells, downregulated markers of inflammation and hepatotoxicity, increased the breakdown of the toxin molecule, and restored phase I and II drug metabolism pathways, including …

GαQ Is A Heterotrimeric G-Protein Subunit That Directs The Selectivity Of Pparγ-Induced Gene Pathways Toward Energy-Related Processes Rather Than Adiposity, Evelyn A. Bates, Zachary A. Kipp, Wang Hsin Lee, Genesee J. Martinez, Sally N. Pauss, Philipp E. Scherer, Terry D. Hinds

Markey Cancer Center Faculty Publications

Background/Objectives: Signaling mediators of PPARγ influence pathways involved in adipogenesis, lipid storage, inflammation, energy-related processes, and glucose utilization. Recent research indicates that PPARγ coregulators, recruited or released during ligand binding, govern specific gene pathways. It was recently discovered that Gα_q, a heterotrimeric G protein subunit, also signals to PPARγ and may significantly affect adipogenesis and glucose sensitivity. Methods: To explore Gα_q’s role in adipocytes, we generated CRISPR-mediated Gα_q (Gnaq) knockout (Gnaq KO) and scramble control cells from 3T3-L1 preadipocytes. Results: The absence of Gα_q resulted in increased lipid accumulation and elevated serine 273 (but …

In Situ Molecular Organization And Heterogeneity Of The Legionella Dot/Icm T4ss, Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen

Markey Cancer Center Faculty Publications

The Dot/Icm type IV secretion system (T4SS) is essential for Legionella pneumophila infection, but its in situ architecture and mechanism remain incompletely understood. Using cryo-electron tomography, we performed subtomogram averaging and 3D classification to resolve structural heterogeneity within the complex. We identified multiple assembly states of the inner membrane complex, including a fully assembled form with a hexamer-of-dimers DotO ATPase and symmetry mismatches between subcomplexes. A composite in situ model revealed a central channel above the inner membrane, likely used for substrate secretion. Imaging of infected macrophages showed T4SSs tethered to host vacuoles and extracellular vesicle release, suggesting additional effector …

Selection Of Rhizobium Strains For Enhanced Dinitrogen Fixation In Forage Legume Production, C Hagedorn, W E. Knight

IGC Proceedings (1977-2023)

A set of procedures for the selection of Rhizobium strains for enhanced dinitrogen fixation in forage legumes is presented. Although forage legumes are among the most efficient members of the Leguminosae regarding biological nitrogen fixation (BNF), they are generally neither productive nor persistent without effective nodulation by appropriate rhizobia. The following methods define a selection and evaluation process to obtain appropriate Rhizobium strains. These include (1) regional survey of the production area, (2) strain collection and soil analyses, (3) strain characterization, (4) strain selection and manipulation, and (5) field evaluation of strains. The initial procedure is to survey the region …

Postoperative Stress Accelerates Atherosclerosis Through Inflammatory Remodeling Of The Hdl Proteome And Impaired Reverse Cholesterol Transport, Dominique M. Boucher, Victoria Lorant, Valerie Rochon, Abigail Carter, Cameron Stotts, My Anh Nguyen, Thomas Laval, Christina Emerton, Nathan Joyce, Nysa Vinayak, Marlena Scaffidi, David Cook, Katey J. Rayner, Rebecca C. Auer, Scott M. Gordon, Mireille I. Ouimet

Physiology Faculty Publications

Over 10 million patients undergoing non-cardiac surgery each year face major cardiovascular complications within 30 days, many due to destabilized atherosclerotic plaques. Reverse cholesterol transport (RCT), driven by HDL and Apoa1, protects against plaque progression, but the effects of surgical inflammation on this pathway remain unclear. Using an abdominal laparotomy model in ApoE^−/− mice on a Western diet, we isolated the impact of surgical inflammation without confounding blood loss. Surgery acutely impaired RCT and cholesterol efflux, with inflammatory remodeling of HDL marked by elevated SAA1/2 and reduced Apoa1. Plaques exhibited higher intracellular lipids, PLIN2 expression, and cleaved caspase-3, indicating …

Brine Solidification And Metals Sequestration Using Lignocellulosics, Frank Kvietok

World of Coal Ash Proceedings

Coal ash has historically been used as a convenient, on-site solidification material for brines generated from flue gas desulfurization at power plants. As alternate utilization scenarios are developed for coal ash, lignocellulosic (wood-based) sorbents can serve as a highly efficient substitute for the solidification process. Results will be shared for a variety of different brine types: thermally reduced, thermally reduced and neutralized, and reverse osmosis. In addition to solidification performance, which demonstrated a 3-4x mass of liquid vs mass of sorbent factor, studies were conducted to quantify the strength of binding to the wood-based sorbent for a broad range of …

Lessons Learned: Commissioning An Fgd Wastewater Treatment System, Bella Triolet, Mckale Grant, Louis Manigault

World of Coal Ash Proceedings

Stantec recently supported commissioning a flue gas desulfurization (FGD) Wastewater Treatment System in accordance with the 2020 Environmental Protection Agency (EPA) Effluent Limitations Guidelines (ELG) Rule, which requires that coal fired power plants meet their more stringent requirements by December 31, 2025. With the addition of the 2024 EPA ELG Rule, coal fired power plants will now be expected to move to zero liquid discharge (ZLD) systems or permanently cease coal combustion by 2034. Due to this change, it is likely that coal fired power plants will be looking to design and install ZLD systems within the next decade. While …

Building Better Partnerships: Design-Build Lessons From The Ash Pond Closure Front Lines, Brian Egan, Nicholas Golden, Mark Edlebeck

World of Coal Ash Proceedings

This presentation shares lessons learned on an ash pond dig-and-haul project delivered by a design-build team with CTI providing engineering and Fisher Contracting delivering construction services. CTI and Fisher Contracting will discuss how they formed their partnership, what makes a good teaming arrangement work, and the challenges that come with working closely together. This team has worked side by side from early design through construction, which has helped cut costs and keep the project on schedule. The talk will show how the partners divide tasks, then adjust roles as needed instead of locking into a fixed plan. In some cases, …

From Demolition To Digital: Using Virtual Reality To Interpret Hamilton House, Hannah R. Miller

Graduate Final Projects

This master’s project investigates the potential of digital reconstruction as a tool for documenting and interpreting demolished historic resources, using Hamilton House as a case study. Employing a mixed-methods approach, the research integrates archival analysis with digital modeling to recreate the spatial context of the house. By grounding the digital model in historical evidence, this study examines how virtual reconstruction can be used to interpret social relationships, patterns of use, and lived experiences that are not fully captured through traditional means of documentation. This project also considers the interpretive implications of reconstructing using fragmentary historical data, addressing the possibilities and …

Establishing A Roadmap For The Planning, Design, And Construction Of A Water Treatment System For A Ccr Unit Closure, Mariah Schroeder, Matthew Balance

World of Coal Ash Proceedings

The treatment of water from a CCR unit is an important step in the closure strategy for surface impoundments to comply with 40 CFR §257.102(d)(2)(i) of the CCR rule. The unwatering and dewatering of a surface impoundment is a necessary first step in ensuring that the eventual closure of the unit will meet the requirements for closure in place described in 40 CFR §257.102(d). Depending on the operational conditions and requirements of the plant, the NDPES discharge limits, and the impacted water quality, the water treatment system needs can vary greatly. This presentation will outline a roadmap for assessment, evaluation, …

Establishing Sustainable Vegetation On A Challenging Legacy Ccr Site, Derek Oosterhouse, Blake Arthur

World of Coal Ash Proceedings

American Electric Power (AEP) is closing and restoring a legacy Coal Combustion Residuals (CCR) site in Ohio that once held a power plant that pioneered innovative steam generation technology. The plant was closed in 1975. Today, the legacy CCR site is being transformed back to natural, sustainable vegetation. To comply with and close the Stormwater Pollution Prevention Plan (SWPPP) permit, permanent stabilization is required on the site. AEP, one of the largest electric companies in the country, holds a core principle to respect the environment through creative sustainable solutions. AEP connected with Profile Products, the world’s largest manufacturer and supplier …

Narrative Power: Strengthening Coal Ash Communications In A Shifting Policy And Media Environment, Tamara Hinton

World of Coal Ash Proceedings

As coal ash management and beneficial use practices continue to advance and regulatory frameworks evolve, the industry still faces a persistent challenge: sharing its story clearly, credibly, and proactively to audiences beyond engineers and regulators. Public perception, media narratives, and stakeholder understanding increasingly influence policy decisions, project timelines, and market opportunities. This session focuses on how strategic communications can serve as a critical tool to support responsible coal ash management and beneficial use. This presentation explores how utilities and industry partners can move from reactive communications to a more deliberate, strategic approach that strengthens trust, mitigates risk, and reinforces the …